• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
• /
• 2009.10a
• /
• pp.93-97
• /
• 2009

Focusing on major event halls in Chung-cheong province, data of events including actual condition of management and state of facilities, accomplishment of performance help us to figure out the problems of local culture events and make concrete plans for development of local culture. Through the facilities and actual result of the performance, records of accidents, including dancing, which could happened in stage allow us to protect performer from an unpleasant matter in advance. Because it is considered to be helpful to make some developments of the art of stage and local culture, we take decisive action with mutual comparison. The purpose of this treatise is to suggest alternative method to develop culture and art events in Chung-cheong province by analyzing the president condition of management.

• Proceedings of the KSAR Conference
• /
• 2003.06a
• /
• pp.61-61
• /
• 2003

We have investigated the novel function of tissue transglutaminase (tTG) in the germinal vesicle breakdown (GVBD) of mouse oocyte. tTG was identified in ooplasm and germinal vesicle by immunostaining assay. Spontaneous maturation of the oocytes elevated in situ activity of tTG by over 2.5 fold at 3 hr, which was determined by a confocal microscopic assay. However, incubation with monodansylcadaverine (MDC), a tTG inhibitor, blocked the activation of tTG. The possible role of tTG in GVBD was investigated by the use of two tTG inhibitors, MDC and cystamine. MDC largely inhibited the GVBD by a concentration dependent manner. GV-stage oocytes were matured to the GVBD stage by 78% at 3 hr in BWW culture medium. However, in the oocytes incubated with MDC for 3 hr, the GVBD rates were 43 and 11% by 50 and 100 mM, respectively. MDC also blocked the entry of 70 kDa TRITC-dextran from the ooplasm to the compartment of germinal vesicle, indicating a possible inhibition of nuclear pore disassembly by MDC. The role of tTG in GVBD was further investigated by microinjection with cystamine. The control oocytes, injected with DPBS, showed about 80 % of GVBD at 3 hr. But the oocytes injected with cystamine showed 15% of GVBD at 3 hr and a little higher rate at 6 hr. In addition, the inhibition of GVBD maturation by MDC was reversible by washing. These results suggested that tTG was involved in the early event of mouse oocyte maturation

• Proceedings of the Korean Society for Cognitive Science Conference
• /
• 2002.05a
• /
• pp.203-207
• /
• 2002

본 연구에서는 한국사람이 모국어인 한국어 단어를 산출할 때와 외국어인 영어 단어를 산출할 때 관여하는 대뇌 영역을 fMRI 를 통해 조사하였다. 또한, 단일 언어를 산출할 때와 두 언어를 수시로 바꾸어서 인출할 때 관련되는 뇌 영역이 어디인지를 조사하였다. 실험에 참가한 피험자는 외국어를 공식적인 교육을 통해 12 세 근처에서 배우기 시작한 대학생이었다. 흔히 분류하는 방식으로 late learner로 구분되는 학생들이었다. 한 피험자가 세 종류의 실험 모두에 참여하였다. 피험자의 실험과제는 그림을 보고 그림에 해당되는 이름을 인출하여 말하는 과제였다. 실험 1, 2, 3 모두에서 사건관련 fMRI(event-related fMRI) 기법을 사용하였다. 실험 1에서는 그림을 보고 그림 이름에 해당되는 한국어 어휘와 외래어 어휘를 산출하게 하였다. 언어관련 뇌영역인 Wernicke 영역, Broca 영역, SMA 영역, SMG 영역 등에서 유의미한 활성화가 있었다. 실험 2 에서는 실험 1 에서 사용하지 않았던 그림을 사용하여 그림의 영어 이름과 외래어 이름을 인출하게 하였다. 외국어인 영어 단어를 산출할 때에도 모국어 단어를 산출할 때와 유사한 영역이 활성화되었다. 특히 외래어 산출 시에는 뇌 활성화 영역이 모국어와 영어 단어 산출할 때와 모국어 산출할 때 활성화되는 공통 영역이 활성화되었다. 모국어 산출과 영어 단어 산출의 차이점은 외국어 산출 시에 활성화 영역이 전반적으로 더 컸다는 것과 외국어 단어 산출 시에 Broca 영역보다 조금 밑쪽에서 그리고 모국어 단어 산출시에는 전전두엽 영역에서 더 많은 활성화가 있었다. 실험 3 에서도 실험 1 과 실험 2 에 사용하지 않았던 그림을 사용하였다. 실험 3 의 특이한 결과는 언어 switching 이 있는 경우에 전통적인 언어 영역 활성화 외에 전전두엽의 활성화가 컸다는 것이다. 아마도 언어를 바꾸어 가면서 단어를 산출하는 것이 전전두엽의 정보선택과정에 많은 영향을 주었던 것으로 해석된다. 전체적으로 어휘 산출시에 모국어 어휘, 외국어 어휘, 외래어 등을 산출할 때 공통되는 언어 영역과 언어 특성적 영역이 활성화된다고 결론지을 수 있을 것 같다.

• Journal of Information Processing Systems
• /
• v.6 no.3
• /
• pp.413-434
• /
• 2010

Wireless sensor networks are usually characterized by dense deployment of energy constrained nodes. Due to the usage of a large number of sensor nodes in uncontrolled hostile or harsh environments, node failure is a common event in these systems. Another common reason for node failure is the exhaustion of their energy resources and node inactivation. Such failures can have adverse effects on the quality of the real-time services in Wireless Sensor Networks (WSNs). To avoid such degradations, it is necessary that the failures be recovered in a proper manner to sustain network operation. In this paper we present a dynamic Energy efficient Real-Time Job Allocation (ERTJA) algorithm for handling node failures in a cluster of sensor nodes with the consideration of communication energy and time overheads besides the nodes' characteristics. ERTJA relies on the computation power of cluster members for handling a node failure. It also tries to minimize the energy consumption of the cluster by minimum activation of the sleeping nodes. The resulting system can then guarantee the Quality of Service (QoS) of the cluster application. Further, when the number of sleeping nodes is limited, the proposed algorithm uses the idle times of the active nodes to engage a graceful QoS degradation in the cluster. Simulation results show significant performance improvements of ERTJA in terms of the energy conservation and the probability of meeting deadlines compared with the other studied algorithms.

• Journal of Korean Neurosurgical Society
• /
• v.50 no.4
• /
• pp.288-292
• /
• 2011

Objective : Matrix metalloproteinases (MMPs), especially MMP-2 and MMP-9 have been known to play an important role in secondary inflammatory reaction after spinal cord injury (SCI). The aim of this study was to investigate the expression and activity of MMP-2 and MMP-9 and to determine their relationship with disruption of endothelial blood-barrier after photochemically induced SCI in rats. Methods : Female Sprague-Dawley rats, weighing between 250 and 300 g (aged 8 weeks) received focal spinal cord ischemia by photothrombosis using Rose Bengal. Expressions and activities of MMP-2 and MMP-9 were assessed by Western blot and gelatin zymography at various times from 6 h to 7 days. Endothelial blood-barrier integrity was assessed indirectly using spinal cord water content. Results : Zymography and Western blot analysis demonstrated rapid up-regulation of MMP-9 protein levels in spinal cord after ischemic onset. Expressions and activities of MMP-9 showed a significant increased at 6 h after the photothrombotic ischemic event, and reached a maximum level at 24 h after the insult. By contrast, activated MMP-2 was not detected at any time point in either the experimental or the control groups. When compared with the control group, a significant increase in spinal cord water content was detected in rats at 24 h after photothrombotic SCI. Conclusion : Early up-regulation of MMP-9 might be correlated with increased water content in the spinal cord at 24 h after SCI in rats. Results of this study suggest that MMP-9 is the key factor involved in disruption of the endothelial blood-barrier of the spinal cord and subsequent secondary damage after photothrombotic SCI in rats.

• Biomedical Science Letters
• /
• v.20 no.4
• /
• pp.237-243
• /
• 2014

Triglyceride (TG) can cause death of macrophages and formation of foam cells thereby increasing inflammation in atherosclerotic plaques. Accumulation of cholesterol in macrophages is another critical event that promotes development of inflammatory cardiovascular diseases. Several proteins are known to transport intracellular cholesterol outside of the cell and these proteins are thought to be protective against atherosclerosis pathogenesis. It is unknown whether TG can affect cholesterol efflux in macrophages. In the current study, we examined mRNA expression levels of genes that promote efflux of cholesterol (ABCA1, ABCG1 and SR-B1). We found that TG treated THP-1 macrophages exhibited an increase in ABCG1 expression in a dose- and time-dependent manner. In contrast, the expression of ABCA1 and SR-B1 remained unchanged. To identify cell signaling pathways that participate in up-regulation of ABCG1, THP-1 macrophages were treated with various cell signaling inhibitors. We found that inhibition of the JNK and p38 MAPK pathway completely abrogated up-regulation of ABCG1 whereas inhibition of MEK1 further enhanced ABCG1 expression in TG treated THP-1 macrophages. Also, TG induced phosphorylation of JNK and p38 MAPK in THP-1 macrophages. These results suggest that TG may potentially influence cholesterol efflux in macrophages.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.23 no.5
• /
• pp.1132-1138
• /
• 2009

Vascular inflammation is an important event in the development of vascular diseases such as tumor progression and atherosclerosis. This study was to investigate the inhibitory effects of WK-38, a new herbal prescription for the treatment of atherosclerosis, on vascular inflammation in human umbilical vein endothelial cells (HUVEC). WK-38 is composed of Rhei Rhizoma, Magonoliae Cortex, Moutan Cortez Radicis. Pretreatment with WK-38 was significantly blocked TNF-$\alpha$-induced expression level of cell adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1), intracellular adhesion molecule-1 (ICAM-1), and endothelial cell selectin (E-selectin) in a dose-dependent manner. TNF-$\alpha$-induced cell adhesion in co-cultured U937 and HUVEC was also blocked by pretreatment with WK-38. Moreover, WK-38 significantly suppressed p65 NF-${\kappa}B$ translocation into the nucleus by TNF-$\alpha$ as well as the phosphorylation and degradation of $I{\kappa}B-{\alpha}$. In conclusion, the present data suggested that WK-38 could suppress TNF-$\alpha$-induced vascular inflammatory process, though inhibition of NF-${\kappa}B$ activation in HUVEC.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.8
• /
• pp.3307-3312
• /
• 2015

The papillary patterned lesion of thyroid may be challenging with many diagnostic pitfalls. Tumor stroma plays an important part in the determination of the tumor phenotype. CD34 is thought to be involved in the modulation of cell adhesion and signal transduction as CD34(+) fibrocytes are potent antigen-presenting cells. Smooth muscle actin (SMA) positivity could be diagnostic for fibroblast activation during tumorigenesis. We aimed to examine the expression of CD34 and alphaSMA in the stroma of papillary thyroid hyperplasia, papillary thyroid carcinoma and papillary tumors of uncertain malignant potential in order to elucidate their possible differential distribution and roles. A total number of 54 cases with papillary thyroid lesions were studied by routine H&E staining, CD34 and ASMA immunostaining. ASMA was not expressed in benign papillary hyperplastic lesions while it was expressed in papillary carcinoma, indicating that tumors have modulated stroma. Although the stroma was not well developed in papillary lesions with equivocal features of uncertain potentiality, CD34 was notable in such cases with higher incidence in malignant cases. So ASMA as well as CD34 could predict neoplastic behavior, pointing to the importance of the stromal role. Differences between groups suggest that the presence of CD34 + stromal cells is an early event in carcinogensis and is associated with neoplasia, however ASMA+ cells are more likely to be associated with malignant behavior and metastatic potential adding additional tools to the light microscopic picture helping in diagnosis of problematic cases with H&E.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.11
• /
• pp.1665-1672
• /
• 2008

Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studies of iSCNT using murine cells and bovine oocytes have been reported, the development of murine-bovine iSCNT embryos beyond the 8-cell stage has not been successful. In this paper, we examined the developmental potential of embryos reconstructed with a murine embryonic fibroblast as the nuclear donor and a bovine oocyte as the cytoplasm recipient. The reconstructed embryos were cultured in CZB (murine medium) or CR1aa (bovine medium). In addition, for the development of a murine-bovine iSCNT blastocyst, the antioxidant ${\beta}$-mercaptoethanol (${\beta}ME$) was supplemented to CR1aa medium. Furthermore, to verify the mouse genome activation in murine-bovine iSCNT embryos, RT-PCR analysis of murine Xist was performed. The development of the murine-bovine iSCNT embryos cultured in CR1aa was significantly higher than that in CZB (p<0.05). With respect to the effect of BME on the development of the murine-bovine iSCNT blastocyst, addition of BME produced a significant increase in blastocyst development (p<0.05). Karyotype analysis confirmed that the reconstructed embryos were derived from murine cells (40XX). The Xist gene was gradually increased from the 8-cell stage to the blastocyst stage. This is the first report of blastocyst development of iSCNT embryos derived from murine somatic cells and bovine oocytes. These results demonstrate that bovine cytoplasm can support the development of later stages of a preimplantation embryo from murine-bovine iSCNT.

• BMB Reports
• /
• v.50 no.1
• /
• pp.1-2
• /
• 2017

Acquiring a selective growth advantage by breaking the proliferation barrier established by gatekeeper genes is a centrally important event in tumor formation. Removal of the mammalian Hippo kinase Mst1 and Mst2 in hepatocytes leads to rapid hepatocellular carcinoma (HCC) formation, indicating that the Hippo signaling pathway is a critical gatekeeper that restrains abnormal growth in hepatocytes. By rigorous genetic approaches, we identified an interacting network of the Hippo, Wnt/${\beta}$-catenin and Notch signaling pathways that control organ size and HCC development. We found that in hepatocytes, the loss of Mst1/2 leads to the activation of Notch signaling, which forms a positive feedback loop with Yap/Taz (transcription factors controlled by Mst1/2). This positive feedback loop results in severe liver enlargement and rapid HCC formation. Blocking the Yap/Taz-Notch positive feedback loop by Notch inhibition in vivo significantly reduced the Yap/Taz activities, hepatocyte proliferation and tumor formation. Furthermore, we uncovered a surprising inhibitory role of Wnt/${\beta}$-catenin signaling to Yap/Taz activities, which are important in tumor initiation. Genetic removal of ${\beta}$-catenin in the liver of the Mst1/2 mutants significantly accelerates tumoriogenesis. Therefore, Wnt/${\beta}$-catenin signaling, known for its oncogenic property, exerts an unexpected function in restricting Yap/Taz and Notch activities in HCC initiation. The molecular interplay between the three signaling pathways identified in our study provides new insights in developing novel therapeutic strategies to treat liver tumors.