• 제목/요약/키워드: Eurema

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Current taxonomic status of Eurema mandarina (Pieridae: Lepidoptera) in Korea

  • Kim, Sung-Soo;Choi, Sei-Woong;Park, Jeong Sun;Kim, Iksoo
    • International Journal of Industrial Entomology and Biomaterials
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    • 제43권1호
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    • pp.6-15
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    • 2021
  • We investigated the taxonomic status of the Korean populations of Eurema hecabe (Linnaeus, 1758) based on morphology and nuclear triose phosphate isomerase (Tpi). Up to now, the Korean population of E. hecabe was classified into E. mandarina (de l'Orza, 1869) based on morphological characteristics. A previous study raised the possibility of the Jeju-do population to be E. hecabe based on the color of forewing's fringe. However, morphological examination showed that the Korean population found in southern areas (Gyeonsang and Jeolla provinces), including Jeju-do is E. mandarina. In particular, the external morphology of autumn seasonal type of the Korean population was almost same as the Japanese mainland population, which is known as E. mandarina. Furthermore, sequence analysis of Tpi gene from nine individuals of E. mandarina collected in South Korean localities including Jeju-do clearly showed that all Korean specimens truly belong to E. mandarina.

Effect of temperature on the development of the Common Grass Yellow, Eurema hecabe

  • Kim, Seonghyun;Park, Haechul;Park, Ingyun
    • International Journal of Industrial Entomology and Biomaterials
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    • 제31권2호
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    • pp.35-39
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    • 2015
  • The developmental responses of insects to temperature are important considerations in gaining a better understanding of their ecology and life histories. Temperature-dependent phenology models permit examination of the effects of temperature on the geographical distributions, population dynamics, and management of insects. Measurements of insect developmental and survival responses to temperature pose practical challenges that depend on the chosen modality, variability among individuals, and high mortality rates near the lower and upper threshold temperatures. Different temperature levels can significantly affect larval development of Eurema hecabe. The development of E. hecabe reared on leaves of Lespedeza cuneata was investigated at three temperature regimes (20, 25, and 30℃), a relative humidity of 60%, and a light:dark photoperiod of 14:10 h. The developmental time from larva to adult was 34.3, 20.6, and 17.9 d at temperatures of 20, 25, and 30℃, respectively. Pupal rate was 47.6%, 47.6%, and 61.9% at temperatures of 20, 25, and 30℃, respectively. The developmental threshold temperature estimated from larva to pupae was 8.1℃ with 381.7 degree-days. There is an increasing need for a standardized manual for rearing this butterfly species based on adequate knowledge of its ecology.

Studies on Ecological Environments and Indoor-rearing Conditions of the Common Grass Yellow Butterfly, Eurema hecabe (남방노랑나비(Eurema hecabe)의 생태환경 및 실내사육 조건에 관한 연구)

  • Lee, Sang-Hyun;Kim, Se-Gwon;Nam, Gyoung-Pil;Son, Jai-Duk;Lee, Jin-Gu;Park, Young-Kyu;Choi, Young-Cheol;Lee, Young-Bo
    • Journal of Sericultural and Entomological Science
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    • 제50권2호
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    • pp.133-139
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    • 2012
  • The Aims of this study are to investigate ecological environments and indoor-rearing conditions of Eurema hecabe for artificial rearing. Two different nesting site, a open area nearby small river(A area; Daejin-ri, Gonyang-myeon, Sachun-si, Gyoungsangnam-do) and a foot of a hill area(B area; Noryang-ri, Seolcheon-myeon, Namhae-gun, Kyoungsangnam-do), selected for investigation of ecological habitat environment, and investigated two times per month, during six month, from June to November in 2010. In result, the number of butterflies, Eurema hecabe, at the hill area(B area) was 6 times more than the open area(A area). We observed the number of eggs, larvae and puapae in the B area more times compared to those in the A area. In the B area, Lespedeza cuneata was higher preference than the other host plant, Lespedeza pilosa and Albizia julibrissin. For development of indoor-rearing conditions, common grass yellow butterfly, Eurema hecabe, was reared in a room condition. As a result of oviposition preference experiment, 100-mated-female laid significantly higher number of eggs $104.9{\pm}19.6$ on Lespedeza cuneata plant, whereas the number of eggs was $12.7{\pm}4.5$ on Cassia obtusifolia plant. Moreover, the eggs hatched within $5.1{\pm}0.9$ days from the day of oviposition and procent of egg hatchability was 53.7% on host plant. The headwidth of each developmental larval stage were $0.36{\pm}0.02$(1st), $0.61{\pm}0.02$(2nd), $0.93{\pm}0.05$(3rd), $1.46{\pm}0.08$(4th), $2.25{\pm}0.11$(5th). The larval period was $12.1{\pm}0.9$ days under high temperature, long day condition($25^{\circ}C$, 16L : 8D), showing 81.0% pupal ratio. The pupal period was $6.9{\pm}0.7$ days, and the emergence rate was 79.6%. Based on above experiment, artificial rearing system of common grass yellow butterfly, Eurema hecabe was completed in indoor condition.

Wolbachia Sequence Typing in Butterflies Using Pyrosequencing

  • Choi, Sungmi;Shin, Su-Kyoung;Jeong, Gilsang;Yi, Hana
    • Journal of Microbiology and Biotechnology
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    • 제25권9호
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    • pp.1410-1416
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    • 2015
  • Wolbachia is an obligate symbiotic bacteria that is ubiquitous in arthropods, with 25-70% of insect species estimated to be infected. Wolbachia species can interact with their insect hosts in a mutualistic or parasitic manner. Sequence types (ST) of Wolbachia are determined by multilocus sequence typing (MLST) of housekeeping genes. However, there are some limitations to MLST with respect to the generation of clone libraries and the Sanger sequencing method when a host is infected with multiple STs of Wolbachia. To assess the feasibility of massive parallel sequencing, also known as next-generation sequencing, we used pyrosequencing for sequence typing of Wolbachia in butterflies. We collected three species of butterflies (Eurema hecabe, Eurema laeta, and Tongeia fischeri) common to Korea and screened them for Wolbachia STs. We found that T. fischeri was infected with a single ST of Wolbachia, ST41. In contrast, E. hecabe and E. laeta were each infected with two STs of Wolbachia, ST41 and ST40. Our results clearly demonstrate that pyrosequencing-based MLST has a higher sensitivity than cloning and Sanger sequencing methods for the detection of minor alleles. Considering the high prevalence of infection with multiple Wolbachia STs, next-generation sequencing with improved analysis would assist with scaling up approaches to Wolbachia MLST.

Comparison of Anti-Oxidative and Cox-2 Promoter Activities of Lepidoptera Extracts (Lepidoptera 추출물에 의한 항산화 및 Cox-2 프로모터 활성 비교)

  • Son, Hyeong-U;Heo, Jin-Chul;Lee, Sang-Han
    • Food Science and Preservation
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    • 제17권5호
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    • pp.752-756
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    • 2010
  • Lepidoptera (butterflies) extracts, traditionally employed as medicines, have various biological activities. Five species of Lepidoptera (Papilio maackii, Papilio xuthus, Pieris rapae, Eurema hecabe, and Sasakia charonda) were extracted with distilled water (DW), dimethyl sulfoxide (DMSO), ethanol (EtOH), and methanol (MeOH). Each extract was analyzed for anti-oxidant and anti-inflammatory activities using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay method, the ferric reducing ability of plasma (FRAP) test, and a cyclooxygenase-2 (COX-2) promoter assay. The results suggest that Lepidoptera extracts have valuable anti-oxidant and anti-inflammatory properties, supporting the idea that the extracts may serve as a food biomaterial(s) preventing oxidative processes and inflammatory damage.

A study on selection of Butterfly and Plant Species for Butterfly Gardening (나비정원 조성을 위한 나비 및 식물 선정 연구)

  • SON, Jinkwan;KONG, Minjae;KANG, Donghyeon;LEE, Siyoung;HAN, Songhee;KANG, Banghun;KIM, Namchoon
    • Journal of Korean Society of Rural Planning
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    • 제21권4호
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    • pp.1-12
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    • 2015
  • Various types of parks such as ecological park, dragonfly park, etc. are tried to design in order to resolve different environmental issues in urban areas. Parks are considered organism habitats that not only improve the biodiversity but also give people a chance to observe living organisms in urban area. This study was conducted to select basic materials for planning a butterfly garden through reference reviews. The following 21 species of butterflies belonged to 1 order, 4 families, and 16 genera were selected: Phengaris teleius, Pseudozizeeria maha, Thecla betulae, Argynnis hyperbius, Dichorragia nesimachus, Hestina assimilis, Polygonia caureum, Sasakia charonda, Vanessa cardui, Vanessa indica, Boloria selene, Byasa alcinous, Papilio machaon, Papilio macilentus, Papilio protenor, Papilio xuthus, Papilio bianor, Sericinus montela, Colias erate, Eurema hecabe, and Pieris rapae. 85 species belonged to 22 families, 59 genera, 74 species, 10 varieties, and 1 subspecies were selected for host and nectar plants for butterflies selected above. These results would be expected to useful in butterfly gardening. After that, we think it is necessary to be applied these study results in field. This study requires a test in the butterfly garden. The test results will be make the butterfly-plant matrix.

Studies on the Insect Fauna of Mt. Gyeryong(I) (계룡산(鷄龍山)의 곤충상(昆蟲相)에 관(關)한 조사연구(調査硏究)(제(第)I보(報)))

  • Chang, Young Duck;Choe, Kwang Ryul
    • Korean Journal of Agricultural Science
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    • 제9권2호
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    • pp.519-539
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    • 1982
  • An preliminary study was made with special references on the insect fauna of Mt. Gyeryong during the period from mid-May to mid-September 1981. The results were obtained as follows; 1. 673 species of insects within 156 families of 20 orders were identified and listed herewith. 2. The 16 species are first records from Korea : Phaneraptera nigro-antennata (Tettigoniidae : Orthop.), Phraraortes kumamotoensis(Phasmidae : Phasm.), Nesogaster lewisi (Nesogasteridae : Dermap), Polymerus palustris(Miridae : Hemip.), Agrosteomela indica (Chrysomelidae:Col.), Monochimus sparsutus (Cermbicidae : Col.), Oberthiiria ialcigera (Bombycidae: Lep.), Alcis albiiera (Geometridae : Lep.), Proplepsis diazama (G eometridae : Lep.) Pandemis cinnamomeana (Tortricidae : Lep.), Hypenodes squalida (Noctuidae : Lep.), Hypolimnas bolina (Nymphalidae : Lep.), Ctenophora nohirae (Tipulidae: Dip.), Ortalotrypeta isshiki (Trypetidaedae : Dip.), Trypeta artemisicola (Trypetidae : Dip.), Ichneumom 8-guttatus ( Ichneumonidae : Hymenop.). 3. We could find a considerable number of Oriental fauna species, Lepismachilis nipponica, Nezara antennata, Parapolybia varia, Anthophora zonata, Parnara guttata, Byasa alcinous, Eurema laeta, Pieris canidia, Eizera maha, Kaniska canace, Danaus sita including two newly recorded species, Monochimus sparstus and Hypolimnas bolina.

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Comparison of butterfly monitoring methods in agricultural landscapes in Korea (우리나라 농촌경관에 서식하는 나비 모니터링 조사 방법 비교 연구)

  • Choi, Sei-Woong
    • Korean Journal of Environmental Biology
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    • 제37권1호
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    • pp.82-87
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    • 2019
  • Global warming has a significant impact on diverse ecosystems including agroecosystem through; changing of phenology, physiology and distribution. Monitoring of biological responses emanating from global warming is required to understand the challenges of biological diversity conservation posed by climate change. The Korean government selected four butterfly species as indicators of climate change in agroecosystem: Papilio xuthus, Pieris rapae, Colias erate, and Eurema mandarina. The aim of this study was to investigate the different monitoring methods of the butterflies in Korea and suggest a suitable monitoring method to track the population trends of butterflies in the agroecosystem. Butterfly monitoring was conducted in eight sites throughout Korea from April to October, 2018 using three survey methods: point census at rice paddy area, point census at the border between rice paddy and hill and line transect along the rice paddy and hill. Each method took approximately 30 min. to count the butterflies. A total of 4,691 butterflies and 92 species were counted: The most dominant species was Pieris rapae with a total count of 1,205 individuals followed by Polygonia c-aureum, Zizeeria maha, Colias erate, Cupido argiades and Papilio xuthus. Among the three census methods, the total number of species and individuals when using line transect method was statistically higher than in the other methods. However, the numbers of the four butterflies indicators showed no difference throughout three census methods. Based on the number of species and the total individuals butterflies in agroecosystem, we advocate for the application of line transect method as it can find more butterflies in agroecosystem. In addition, we advised for the implementation of education programs on the line transect method in butterfly identification to participants of the national monitoring program.