• Journal of Applied Biological Chemistry
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• v.48 no.2
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• pp.84-88
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• 2005

To develop functional food and anti-obesity drug through inhibition of dietary lipid absorption, inhibitory effects of herb extracts on pancreatic lipase were investigated. Due to high yield and simplicity of isolation, lipase inhibitor (ELI) was isolated from ethyl acetate extract of Eugenia aromaticum, which showed highest inhibitory activity, and characterized for development of novel functional material. Stability of ELI at high temperature and low pH was investigated. Results showed ELI is relatively stable under thermal and acidic conditions, reversible, and noncompetitive inhibitor of pancreatic lipase.

• Preventive Nutrition and Food Science
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• v.10 no.1
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• pp.81-87
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• 2005

The objective of this study was to determine the radical scavenging activity, total phenolic content, antimicrobial activity, minimum inhibitory concentration (MIC) of ethanol extracts of 32 medical plant species that have been commonly used in medicinal plants. Total phenolic index of T. chebula exhibited the highest value (498.01㎎/g), followed by R. coreanus miquel (400.33㎎/g), Sanguisorba officinalis (368.25㎎/g), P. thumbergiana (259.74㎎/g) and Eugenia aromaticum (229.38㎎/g). Radical scavenging activity for the DPPH radical was highest in T. chebula (40.91%, p<0.01), followed by C. sappan (36.50%), S. officinalis (32.92%), R. coreanus miquel (26.54%) and P. thumbergiana (24.50%). The extracts from T. chebula, R. coreanus muquel, C. sappan, E. aromaticum, S. officinalis and C. japonica possessed outstanding antimicrobial activity against Escherichia coli, Salmonella Typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis and Lactobacillus plantarum. MIC was determined on those extracts that showed high efficacy against the test organisms. The most potent MIC values were seen for T. chebula extract against P. aeruginosa, S. aurusa, E. coli, B. subtilis, L. plantarum and S. Typhimurium at 7.8, 7.8, 15.6, 7.8, 125 and 31.2㎍/mL, respectivley. Furthermore, the total phenolic content and radical scavenging activity were very closely correlated for all samples (r=0.78). The coefficient correlations between total phenolic index and antimicrobial activity were 0.91 (E. coli), 0.91 (B. subtillis), 0.79 (P. aeruginosa), 0.79 (S. Typhimurium) and 0.70 (L. plantarum).

• The Plant Pathology Journal
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• v.24 no.2
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• pp.178-182
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• 2008

Five essential oils and 15 herbal extracts were evaluated to control Bursaphelenchus xylophillus in laboratory. The essential oils from clove plant (Syzygium aromaticum), mustard (Brassica integrefolia), thyme (Thymus vulgaris), and Pelargonium inquinans were found to be highly promising and gave excellent control of the nematodes at all the time of exposure. Among them, the least one gave 91.3% mean mortality rate at 24 hours of exposure time, which is highly significant from the control. While in the second study, most of the methanol (Desmodium caudatum, Paulownia coreana, Auckulandia lappa, Sophota flavescens, Aloe sp., Rheum palmatum, Zingiber officinale, Magnolia officinalis, and Eugenia caryophyllata), hexane (Torreya nucifera, Pharbitis nil, Prunus mume, Melia azedarach, and Xanthium strumarium), and hot water (Cinnamomum cassia) herbal extracts killed the nematodes, but in varying degrees compared to the control. Only one extract was found to be promising viz Magnolia officinalis which found to be statistically different from the control and gave mean mortality of 72, 82.3, and 85.3 % for 24, 48, and 72 hours exposure, respectively. Further screening was conducted for M. officinalis with concentrations of 1,000, 100, and 10 ppm against the same species of nematode with the same time of exposure. However, it gave an excellent result for 1,000 ppm for all time of exposure, whereas for the 100 and 10 ppm it gave mean mortality of 39.5 and 25.8% for the time 72hrs, respectively that were statistically different from the control.

• Journal of Life Science
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• v.13 no.5
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• pp.604-617
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• 2003

Candida albicans is one of the most common etiological agents in fungi-associated skin infections. There is an increase of candidiosis especially in the patient of acquired or induced immunodeficiency syndromes or in the event of long-term antibiotics and immuno-suppressor or cytotoxic therapies. To screen out reliable and effective anti-candidiosis agent, in this study, we have evaluated antifungal activity of 298 plant extracts against C. albicans. Based on the results of disc-paper method and determination of minimal inhibitory concentration, fifteen extracts were finally selected as possible sources of anti-candidiosis agent. Especially, six different plant extracts, such as Rubus parvifolius, Euphorbia pekinensis, Coptis chinensis, Eugenia aromaticum, Paeonia lactiflora var. hortensis and Paeonia suffruticosa showed strong antifungal activity against C. albicans, not to S. cerevisiae. These results suggested that medicinal and wild plants could be the potential source of antifungal agent.

• Journal of Life Science
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• v.18 no.7
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• pp.974-979
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• 2008

The powdery mildew, a fungal plant disease found in varieties of plant cultures, is occurred by attack with Fusarium sp., Sphaerotheca sp., Leveilluna sp., and Eryshipe sp.. In this study we investigated the control of Fusarium graminearum, a barley powdery mildew fungus, by natural plant extracts. Among the 900 plant extracts tested, Zanthoxylum schinifolium, Ligusticum acutilobum, Bidens frondosa L., Dictamnus dasycarpus, Evodia officinalis, Disporum sessile, Scopolia japonica Max., Styrax japonica S. et Z., Dictamnus dasycarpus Turcz., Sinomenium acutum Rehder et Wils., Eugenia aromaticum, Rubus parvifolius L., Reynoutria elliptica, Coptis chinensis, Paeonia lactiflora Pall., Rheum undalatum, Paeonia suffruticosa, Oenothera odorata Jacq., Euphorbia pekinensis Rupr., and Nepeta cataria were selected based on spore germination inhibition assay. Further mycelial growth inhibition assay with economical and safety considerations led us to finally select Z. schinifolium (sancho) for control of F. graminearum. To produce antifungal sancho extract, methanol was suitable for extraction and subsequent fractionations of the extract showed that the water residue mainly had antifungal activity. The sancho extract and its fractions showed minor antibacterial activity against different pathogenic or food spoilage bacteria, but they did not show any harmful effects against young tomato plant by treatment of $1,000\;{\mu}g/ml$ in green chamber test. These results suggested that the extract of sancho has high potentials on control of a powdery mildew fungus, F. graminearum.