• Title/Summary/Keyword: Essential amino acids

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Development of transgenic potato with high content of sulphur-containing essential amino acids (황 함유 필수아미노산이 증대된 기능성 형질전환 감자 개발 현황)

  • Goo, Young-Min;Kim, Tae-Won;Lee, Min-Kyung;Lee, Shin-Woo
    • Journal of Plant Biotechnology
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    • v.40 no.1
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    • pp.1-8
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    • 2013
  • Potato is the 4th important crop along with rice, wheat and maize. It contains high quality of starch with relatively high content of vitamin C and protein. However, there is a nutritionally limiting factor due to a low level of sulphur-containing essential amino acid including methionine and cysteine. Recently, recombinant DNA technology and metabolic engineering with genes involved in the bio-synthetic pathway have been applied to enhance the level of these essential amino acids. In this report, it has been discussed about the current status and bottleneck on the development of transgenic potato containing high level of sulphur-containing essential amino acids.

Effect of Feeding Yeast (Saceharomyces eerevisiae) on Growth Performance and Changes of Intestinal E. coli in Broiler Chicks (효모의 급여가 육계의 성장 및 장내 대장균의 변화에 미치는 영향)

  • 이현우;김인호;김춘수;손중천
    • Korean Journal of Poultry Science
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    • v.24 no.2
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    • pp.67-72
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    • 1997
  • In order to investigate the effect of feeding live yeast (Sacckaromyces cerevisiae) on the growth performance and changes of intestinal microorganism (E. coli), a growth assay was conducted with 144 broiler chicks. Treatments were consisted of corn-soybean meal control, 0.05% live yeast, and 0.05% dead yeast. Most of the chick protein of the live yeast was in the pure protein form, and had a high amino acid composition with 47% of essential amino acids and 53% of non-essential amino acids. No differences in growth performance were shown among dietany treatments. Total number of E. coli in the small intestine of chicks fed either live or dead yeast was significantly reduced compared to chicks fed the control diet. Although the changes of E. coli in the cecum were not identical to differences in the small intestine, the changes of E. coli in the cecurn had a similar trend.

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Quality Characteristics of Fish, Crab and Red-Crab Stock Prepared by High Pressure Extract Method (고압 가열 추출 방식을 이용한 생선 육수, 붉은 대게 육수, 대게 육수의 품질 특성)

  • Bae, Gum-Kwang;Byun, Gwang-In;Cho, Soo-Keun
    • Culinary science and hospitality research
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    • v.13 no.4
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    • pp.293-304
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    • 2007
  • Using the high-pressure heating extraction process suitable for mass-production, crab stock(CS), red crab stock(RCS) and fish stock(FS) were manufactured and their quality and organic features were compared. The results are as follows. In mineral content, the content of essential amino acids, the content of delicious amino acids and preference, CS and RCS were rated higher than FS, and especially RCS had the most abundant essential amino acid content and had the highest rating in organic evaluation. Also, in terms of cost, RCS was more economical than regular CS, which is relatively expensive. Therefore, it is believed that replacing fish bones with RCS in making stock will be more cost-saving and have higher preference.

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On The Amino Acid Compositions of The Korean Cereal Proteins (한국산곡류단백질(韓國産穀類蛋白質)의 아미노산(酸) 조성비율(組成比率)에 관(關)한 연구(硏究))

  • Sung, Nak-Eung;Kang, Hi-Ryun
    • Journal of Nutrition and Health
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    • v.3 no.2
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    • pp.113-117
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    • 1970
  • 1) It was found that the polished rice is short of Iysine, methionine, cystine, and tryptophan among the essential amino acids, and that it shows no significant variations with regard to its pattern of geographical region. 2) Comparing in the contents of essential amino acids in the cereals, such as polished barley, wheat, great millet, and soy bean, the soy bean is found to be better than the polished rice, while the others to be either poorer or similar than the polished rice. 3) The aminal experiments observed with the mixed diet revealed that the most ideal composition of the mixed diet is the one composed of 70% polished rice, 20% polished barley, and 10% soy bean.

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Food Components of Coho Salmon and Rainbowtrout (은연어와 무지개 송어의 식품성분)

  • 김경삼;최영준
    • The Korean Journal of Food And Nutrition
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    • v.6 no.2
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    • pp.73-80
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    • 1993
  • In order to examine food components of coho salmon and rainbow-trout, We analyzed the composition of protein, amino acids and total lipids. The coho salmon muscle contained about 19.3% of protein with the composition of 29.9% in sarcoplasmic protein, 56.3oA in myofibrillar protein 12.5% alkali soluble protein and 2.6% in stroma. Those of rainbow-trout contained 34.1%, 56.4%, 8.3% and 2.9%, respectively. The sarcoplasmic and myofibrillar protein were composed of 13 subunits in coho salmon, and 16 and 15 subunits in rainbow-trout. Judging from the contents of essential amino acids, both muscle proteins were complete proteins. The most remarkable feature of free amino acids was that a large amount of dipeptide anserine was present with fairly lower levels of 1 methyl histidine, taurine, histidine, alanine and glycine in both muscle extracts. The total fatty acids of coho salmon was composed of 31.49% polyenes, 43.79% monoenes and 24.73% saturates. The composition of total fatty acid of coho salmon muscle was not different from that of rainbow-trout muscle.

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Changes in Amino Acids and Fatty Acids Composition during Germination of Rapeseed (유채(油菜) 발아중(發芽中) 아미노산과 지방산 조성(組成)의 변화(變化))

  • Cho, Byoung-Mi;Yoon, Suk-Kwon;Kim, Woo-Jung
    • Korean Journal of Food Science and Technology
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    • v.17 no.5
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    • pp.371-376
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    • 1985
  • Rapeseeds (Brassica napus L.)were germinated at $25^{\circ}C$ for 60 hours under dark condition in order to investigate the compositional changes in amino acids and fatty acids. The weight loss was most significant after 30 hours of germination and root growth was observed from that time. Analysis showed that Glu, Lys and Asp were the major amino acids and Ile was the limiting one. Germination caused an increase in Lys, Thr, Val and Leu while Met and Phe decreased among essential amino acids, resulting Met to be the limiting amino acid. The major fatty acids were oleic (49.3%), linoleic (22.0%) and eicosenoic (10.5%) acids. The decrease in oleic and stearic acids was measured while linoleic, linolenic, eicosenoic and erucic acids showed initial decrease followed by gradual increase.

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Characterizing Sections of Elk Velvet Antler by pH and Mineral, Fatty Acid, and Amino Acid Composition

  • Dong-Kyo Kim;Sang-Hoon Lee;Eun-Do Lee;Hye-Jin Kim;Jinwook Lee;Sung-Soo Lee;Aera Jang;Kwan-Woo Kim
    • Journal of Food and Nutrition Research
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    • v.9 no.3
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    • pp.170-176
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    • 2021
  • This study was conducted to determine the composition change in different sections of elk velvet antler at 90 days. The following parameters were analyzed: moisture, crude protein, crude fat, crude ash, crude fiber, pH, minerals (Ca, P, K, Mg, Fe, Mn, Zn, Cu, and Pb), amino acids, and fatty acids. Dry matter, crude fiber, and crude ash contents were higher in the base of the antlers and lower in the tip. In contrast, crude protein and crude fat contents were highest in the tip. Moisture content was high in the upper and medium sections of the antlers, but the difference was not significant. Calcium (Ca), phosphorus (P), and magnesium (Mg) contents were high in the base of the antlers, potassium (K) content was high in the tip of the antlers, and zinc (Zn) content showed no difference between antler sections. Saturated fatty acid content was highest in the base of the antlers, whereas unsaturated fatty acid content was highest in the tip. Among unsaturated fatty acids, monounsaturated fatty acid content was high in the tip of the antler, whereas polyunsaturated fatty acid content was high in the upper section of the antler. Essential amino acid content was highest in the upper section of the antler, whereas non-essential amino acid content was lowest in the base. Crude protein, crude fat, K, and unsaturated fatty acid content tended to be high in the tip of the antler, and essential amino acid and polyunsaturated fatty acid contents tended to be high in the upper section of the antler. The tip of the antler had the highest nutrient content. In order to prevent cardiovascular disease, consumption of a certain amount of polyunsaturated fatty acids and amino acids from the upper section of the antler could be beneficial in terms of pharmacological efficacy.

Changes of Nitrogen Compouds and Free Amino Acid of Mung-bean Sprout (녹두나물 성장과정중의 실소화합물과 유리아미노산의 변화에 관한 연구)

  • 김경자
    • Journal of the Korean Home Economics Association
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    • v.19 no.1
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    • pp.25-31
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    • 1981
  • The changes of various nitrogenfractions and free amino acid composition were investigated at various growing stages. The results are summarized as follows 1) Total nitrogen, water soluble protein nitrogen, and true protein nitrogen were decreased with growth. 2) Free amino acid increased with sprout-growth. the content of free amino acid were about 74.4%(combined Ser, Asp, Arg, Val) after 4 day sprout. It is belived that serine, aspartic acid, arginine and valine play an important role as taste compounds in mungbean sprout. 3) 16kinds of amino acid, including essential amino acids in human nutrition except tryptophan and cystin were quantified.

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Existence of Amino Acids in Defined Culture Medium Influences In Vitro Development of Parthenogenetic and Nuclear Transfer Porcine Embryos

  • Won, Cheol-Hee;Park, Sang-Kyu;Kim, Ki-Young;Roh, Sang-Ho
    • Journal of Embryo Transfer
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    • v.23 no.4
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    • pp.245-250
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    • 2008
  • This study was designed to investigate the effect of essential amino acids (EAA) and/or non-essential amino acids (NEAA) on the development of parthenogenetic and somatic cell nuclear transfer (SCNT) porcine embryos in vitro. To evaluate the timing of amino acids supplementation, activated oocytes were cultured in NCSU23-PVA with EAA, NEAA or NEAA+EAA (AAs) during specific periods as below: EAA, NEAA or AAs were supplemented during Day 0 to 6 (whole culture period: ALL), Day 2 to Day 6 (post-maternal embryonic transition period: POST-MET), Day 5 to Day 6 (post-compaction period: POST-CMP), Day 0 to Day 2 (pre-maternal embryonic transition period: PRE-MET), or Day 0 to Day 4 (post-compaction period: PRE-CMP). Supplementation of NEAA decreased cleavage rates in PRE-MET and PRE-CMP and also decreased blastocyst rates in POST-CMP. On the other hand, EAA significantly enhanced blastocyst formation rate in POST-MET and no detrimental effect on embryonic development in other groups. Interestingly, NEAA and EAA had synergistic effect when they were supplemented to the medium during whole culture period. Supplementation of AAs also enhanced SCNT porcine embryo development whereas BSA-free medium without AAs could not supported blastocyst formation of SCNT embryos. In conclusion, existence of EAA and NEAA in defined culture medium variously influences the development of parthenogenetic and SCNT porcine embryos, and their positive effect are only occurred when both EAA and NEAA are supplemented to the medium during whole culture period. Additionally, AAs supplementation enhances the blastocyst formation of SCNT porcine embryos when they are cultured in the defined condition.

Effect of fermented biogas residue on growth performance, serum biochemical parameters, and meat quality in pigs

  • Xu, Xiang;Li, Lv-mu;Li, Bin;Guo, Wen-jie;Ding, Xiao-ling;Xu, Fa-zhi
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.10
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    • pp.1464-1470
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    • 2017
  • Objective: This study investigated the effect of fermented biogas residue (FBR) of wheat on the performance, serum biochemical parameters, and meat quality in pigs. Methods: We selected 128 pigs (the mean initial body weight was $40.24{\pm}3.08kg$) and randomly allocated them to 4 groups (1 control group and 3 treatment groups) with 4 replicates per group and 8 pigs per pen in a randomized complete block design based on initial body weight and sex. The control group received a corn-soybean meal-based diet, the treatment group fed diets containing 5%, 10%, and 15% FBR, respectively (abbreviated as FBR5, FBR10, and FBR15, respectively). Every group received equivalent-energy and nitrogen diets. The test lasted 60 days and was divided into early and late stages. Blood and carcass samples were obtained on 60 d. Meat quality was collected from two pigs per pen. Results: During the late stage, the average daily feed intake and average daily gain of the treatment groups was greater than that of the control group (p<0.05). During the entire experiment, the average daily gain of the treatment groups was higher than that of the control group (p<0.05). Fermented biomass residue did not significantly affect serum biochemical parameters or meat quality, but did affect amino acid profiles in pork. The contents of Asp, Arg, Tyr, Phe, Leu, Thr, Ser, Lys, Pro, Ala, essential amino acids, non-essential amino acids, and total amino acids in pork of FBR5 and FBR10 were greater than those of the control group (p<0.05). Conclusion: These combined results suggest that feeding FBR could increase the average daily gain and average daily feed intake in pigs and the content of several flavor-promoting amino acids.