• 한국식품과학회지
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• 제32권4호
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• pp.772-787
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• 2000

In this study, evaluation on the loaches from the various sources was carried out in respect of their taste compounds. The samples were classified by local, season and growing condition and evaluated in terms of composition, the refuse, contents of nucleotides and their related compounds, total and free amino acid, organic acid and fatty acid. Evaluation on the compositions revealed the fact that, in terms of the local, the imported loach contain a low level of fat and high level of ash, in comparison with Korean loach; in terms of growing condition, cultured loach contains a high level of fat and low level of moisture, in comparison with wild loach; and in terms of the season, the summer loach contains less moisture than the fall loath; but as for the contents of other component, the summer loach was superior. As for the refuse, the imported loach had a greater refuse than the Korean loach. In the growing condition, the wild loach had lower value than the cultured loach. There was no substantial difference in each season. Also nucleotide and these related compounds were detected in all of the samples, while ATP was not found. The content levels of such detected compound did not show a substantial difference in each condition. However, generally, the imported loach was poor in such compound and all of the samples were high in IMP. The samples were high in total amino acids. The local and growing conditions showed no substantial difference, while in the season, the summer loach had a higher content of total and essential amino acid than the fall loach. As for the contents of free amino acids, similar distribution of the composition was shown in each sample, even though there was little difference in their constituents. Also, total content of free amino acid varied with the conditions of the samples. As for total content of organic acid and the distribution of the composition, there was a little difference between each condition. In the season and growing condition, the summer loach was high in organic acid. As for the distribution of the fatty acid compositions, there was no substantial difference between each condition and each sample. In the case of the wild loach, the summer loach was high in ${\omega}-3$ polyunsaturated fatty acid and the fall loach was high in essential fatty acid. Similarly, in the case of the imported loach, the summer loach was high in ${\omega}-3$ polyunsaturated fatty acid and the fall loach was high in essential fatty acid.

• 동아시아식생활학회지
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• 제4권3호
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• pp.103-109
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• 1994

Changes in taste components of Backilju, an traditional alcoholic beverage of Korea, were investigated. Ethanol(17%) was the most abundant, and then isoamylalcohol(23mg%) and methanol(8mg%) were also detected in a small amount in Backilju. Major non-volatile organic acids were lactic and malic acid, followed by citric, fumaric and succinic acid. Arginine, phenylalanine and glutamic acid were major free amino acids and essential amino acid content was 230∼560 mg%, which was 45∼48% of total free amino acids. The major fatty acid of total lipid was palmitic acid(37∼43%). It has been found that the Backilju contained more free amino acids and alcohols than other Korean Yokjus.

• 생명과학회지
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• 제19권2호
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• pp.192-197
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• 2009

브로콜리 싹의 생리활성 기능과 이용 가능성에 관한 연구의 일환으로 브로콜리 싹의 일반성분 및 영양성분을 측정한 결과는 다음과 같다. 일반성분은 건물(dry basis)을 기준으로 수분 함량은 2.04%, 조단백질 22.04%, 조지방 12.80%, 조회분 6.25% 및 탄수화물 56.87%를 함유하였다. 구성당은 ribose가 가장 많았고 다음으로 fructose, glucose 순으로 총 3종이 검출 되었다. 아미노산은 lysine 함량이 691.09 mg%로 가장 많이 함유되었고, 다음으로 leucine, aspartic acid, glutamic acid, arginine, phenylalanine, valine 순으로 검출 되었다. 브로콜리 싹의 구성 지방산 중 포화지방산 함량은 palmitic acid가 가장 높았으며 arachidic acid, lignoceric acid, behenic acid 순으로 나타났다. 불포화지방산은 cis-11,14-eicosatrienoic acid가 가장 높았으며 linolenic acid, linoleic acid, oleic acid 순으로 나타났다. 유기산은 총 3종의 유기산이 검출되었으며, 이 중 lactic acid가 가장 많았고, 다음으로 malic acid, citric acid 순으로 검출되었다. 비타민 A와 E의 함량은 각각 0.06 mg%와 0.82 mg%이었다. 무기질은 총 8종의 무기질 성분이 검출되었으며, 이 중 K 함량이 가장 많았으며, 다음으로 Mg, Na, Fe 순이었고 Zn, Mn, Cu의 함량은 미량이 었다. 이상의 결과 브로콜리 싹은 필수아미노산 및 필수지방산을 비롯한 항산화 비타민과 무기질을 다량 함유하고 있어 브로콜리 싹의 식품 재료로의 이용화가치가 한층 더 높아질 것으로 기대되어지며, 브로콜리 싹 분말을 이용한 다양한 조리법의 개발과 더불어 가공식품 및 기능성 식품으로의 제품 개발이 필요하다.

• 한국축산식품학회지
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• 제28권5호
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• pp.529-534
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• 2008

본 연구는 아연을 주로 하는 이온수와 규소 성분이 주인 복합광물질을 급여시 돈육의 일반성분, 식육 콜레스테롤, 지방산 및 아미노산 조성에 미치는 영향에 대해 조사하였다. CON(0%), T1(이온수)및 T2(이온수 + 복합광물질)로 처리하였으며, 출하 전 66일간 사육하였다. 일반성분 및 콜레스테롤 함량은 유의적인 차이는 나타나지 않았다. 지방산조성의 경우 다른 처리구에 비해 T2의 palmitic acid, linoleic acid, arachidonic acid가 유의적으로 높게 나타난 반면 oleic acid는 유의적으로 낮게 나타났다. 포화지방산(STA)의 경우 T2(28.54%)가 유의적으로 높은 함량을 나타내었고, 불포화지방산(UFA) 및 UFA/SFA의 경우 유의적으로 낮게 나타났다. 하지만, 필수지방산(EFA) 및 EFA/UFA은 T2가 유의적으로 높게 나타났다. 아미노산 조성 중 다른 처리구에 비하여 glutamic, alanine, leucine, lysine은 T1이 유의적으로 높게 나타났으며, serine, valine, isoleucine은 T2가 유의적으로 높은 함량을 나타내었다. 또한, 총 아미노산 함량 및 필수아미노산(EAA)는 T1처리구가 유의적으로 높게 나타났다. 이상의 결과 이온수 및 복합광물질의 급여가 돼지의 지방산중 SFA를 증가시키고, UFA를 감소시켰으며, 필수아미노산의 함량을 증가시킴을 증가시켜 알 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제21권7호
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• pp.697-702
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• 2011

A strain of the cyanobacterium Arthrospira was isolated from Lake Chahannaoer in northern China and was characterized according to microscopic morphology, photosynthetic oxygen-evolving activity, growth rate, and nutritional profile. Compared with thermophilic Arthrospira species occurring naturally in tropical and subtropical lakes, this isolate is mesophilic and grows optimally at ${\sim}20^{\circ}C$. The total protein, fatty acid, phycocyanin, carotenoid, and chlorophyll a contents were 67.6, 6.1, 4.32, 0.29, and 0.76 grams per 100 grams of dry weight, respectively. The strain is rich in polyunsaturated fatty acids (PUFAs). An essential omega-3 fatty acid, docosahexaenoic acid (DHA), was detected, and ${\gamma}$-linolenic acid (GLA) and DHA accounted for 28.3% of the total fatty acid content. These features of this newly isolated strain make it potentially useful in commercial mass culture in local areas or as a biofuel feedstock. It is also an alternative resource for studying the metabolic PUFA pathways and mechanisms of cold stress tolerance in cyanobacteria.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제16권3호
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• pp.153-161
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• 2013

Polyunsaturated fatty acids (PUFAs) are the major components of brain and retina, and are the essential fatty acids with important physiologically active functions. Thus, PUFAs should be provided to children, and are very important in the brain growth and development for fetuses, newborn infants, and children. Omega-3 fatty acids decrease coronary artery disease and improve blood flow. PUFAs have been known to have anti-inflammatory action and improved the chronic inflammation such as auto-immune diseases or degenerative neurologic diseases. PUFAs are used for metabolic syndrome related with obesity or diabetes. However, there are several considerations related with intake of PUFAs. Obsession with the intake of unsaturated fatty acids could bring about the shortage of essential fatty acids that are crucial for our body, weaken the immune system, and increase the risk of heart disease, arrhythmia, and stroke. In this review, we discuss types, physiologic mechanism of action of PUFAs, intake of PUFAs for children, recommended intake of PUFAs, and considerations for the intake of PUFAs.

• 한국응용과학기술학회지
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• 제5권2호
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• pp.9-15
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• 1988

In order to investigate the amino acid and fatty acid content in Thuja biotae water extract treated with alkaline, it was performed. There are 16 kinds of different amino acid and 20 kinds of different fatty acid in Thuja biotae water extract. An aspartic acid was contained 52％ and proline was contained 10％, particulary, r-aminobutyric acid was analysed. Essential fatty acids; linoleic acid, linolenic acid and arachidonic acid were cotained a lot amount. There are 11 different unknown materials which were identified by GC-MS spectrum, such as N-[($4{\alpha},5{\alpha}$)-cholestan-4-yl]-acetamide; 22,26-Epithio-furost-5-en-3-ol; 2-Methyl-6-(4-methyl-3-cyclohexen-1-yl)-4-heptanone; 3,12,14-Tris(acetyloxy)-pregnane-15,20-dione;22-Methyl-26-thio-furost-5-en-3-ol; 7-Ethenyl-1,2,3,4,4a,4b,5,6,7,9,10,10a-dodecahydro-1,4a,7-trimethyl-1-phenanthrene carboxyaldehyde; Methoxyiminopro-panedioic acid; 13-Methyl-13-${\beta}$-Methyl-13-vinyl-dodecaarp-7-en-3-3-ol; 22-Methyl-26-thio-furost-6-methyl-3-ol; $5{\alpha}-Androstane-2$,11-dione; 9-Methyl-heptadecanoic acid.

• Clinical and Experimental Pediatrics
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• 제45권9호
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• pp.1083-1089
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• 2002

목 적 : 지속적으로 증가하는 소아 비만은 성인 비만으로 이행되기 쉽고 합병증으로 고혈압, 지방간, 동맥경화증이 동반될 수 있다. Carnitine은 장쇄 지방산이 미토콘드리아로 이동할 때 필요한 조효소로 지방산 대사에서 중요한 역할을 한다. 지방간을 가진 비만 소아에서 혈중 지방산과 carnitine 농도를 측정함으로써 L-carnitine을 임상적으로 비만 치료에 적용할 수 있는지를 알아보고자 본 연구를 실시하였다. 방 법 : 7-18세의 지방간으로 진단받은 비만아 9명과 정상 대조군 소아 10명을 대상으로 하였다. 혈장을 sodium borate를 섞어 원심분리 후 하층을 methylene chloride를 이용하여 계층 분리하였고, MSTFA와 acetonitrile을 넣고 유도체화 반응을 시켰다. GC-MS 자동 분석기로 혈청 지방산 fraction을 정량 분석하였고, carnitine(free, acyl, total)은 cycling technique을 이용하여 415 nm에서 정량 분석하였다. 결 과 : 혈중 총 지방산은 지방간이 동반된 비만아 군에서 대조군에 비해 유의하게 증가하였고 특히 장쇄 지방산(myristic acid, palmitoleic acid, palmitic acid, linoleic acid, oleic acid, stearic acid)이 의미 있게 증가되어 있었다. 총 carnitine과 유리 carnitine 농도가 비만아에서 정상아에 비해 유의하게 증가하였으나 acyl carnitine은 유의한 차이가 없었다. 결 론 : 지방간이 동반된 비만아에서 장쇄 지방산이 뚜렷이 증가되었으며, 비만아군에서 정상아에 비해 혈청 carnitine이 증가되어 있음을 알 수 있었다. 이것을 기초로 하여 비만아에서 L-carnitine 투여 후 지방산 대사의 변화에 대해 추후에 연구할 예정이다.

• Journal of Animal Science and Technology
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• 제50권2호
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• pp.247-254
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• 2008

경남 함양지방의 양돈장에서 사육중인 아메리칸 버크셔종을 대상으로 산머루주 부산물을 배합사료와 혼합한 시험사료 5%를 20두에 대해 생시체중 70 kg에서 88일간 급여한 후 등심부위를 채취하여 품질평가를 실시하였다. 일반성분 분석결과 수분과 조지방은 대조구와 처리구 간에 유의적인 차이를 나타내지 않았으나(p>0.05), 조단백질과 조회분은 처리구가 낮았다(p<0.05). pH와 WHC는 대조구와 처리구 간에 차이가 없었고(p>0.05), 콜레스테롤과 지방의 전단가는 처리구가 낮게 나타났다(p<0.05). 육색은 차이가 없었으나(p>0.05), 지방색에서의 명도와 백색도는 처리구가 높았다(p<0.05). Oleic acid(C18:1), linoleic acid(C18:2, n-6), arachidonic acid(C20:4, n-6), 필수아미노산 및 EFA/UFA에서 유의적인 차이가 나타났다(p<0.05). 총 아미노산 함량은 대조구와 처리구 간에 유의적인 차이가 없었으나(p>0.05), 필수아미노산은 처리구가 대조구 보다 높게 나타났다(p<0.05). 이상에서 산머루주 박 급여 시 등지방색, 전단가, 콜레스테롤 및 필수지방산의 함량이 개선되는 것으로 나타나 산업적 응용 가능성이 있을 것으로 판단되었다.

• KSBB Journal
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• 제26권6호
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• pp.557-560
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• 2011

It has been known that Δ-desaturase (TAD5) in the biosynthetic pathway of long chain polyunsaturated fatty acids of Thraustochytrium aureumis responsible for the conversion of di-homo-${\gamma}$-linolenic acid (C20：4) into arachidonic acid (C20：4). The genetic sequence analysis on TAD5 of Thraustochytrium aureum ATCC34304 used in this study showed that it has two amino acid changes when compared to that of Thraustochytrium aureum TAD5 first reported in 2003. Accordingly, Thraustochytrium aureum ATCC34304 TAD5 was named TAD5_1. TAD5_1-inserted methylotropic Pichia pastoris was prepared and then cultured with a precursor fatty acid, di-homo-${\gamma}$-linolenic acid. GC analysis confirmed that a certain amount of the precursor fatty acid was converted into arachidonic acid. In this study, not only a recombinant Pichia pastoris with the typical activity of ${\Delta}5$-desaturase which plays an essential role in the biosynthesis of LCPUFAs was successfully made but also the preparationpotential of a recombinant Pichia pastoris strain which may synthesize eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) that are important in maintaining and improving human's brain function was proposed.