• Applied Biological Chemistry
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• 제24권2호
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• pp.94-100
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• 1981

콩나물제조중(製造中) 단백질(蛋白質)의 영양가(營養價)를 화학적(化學的)으로 평가(評價)하기 위해서 대두중(大豆中)의 아미노산조성(組成)과 이들대두(大豆)가 발아하여 콩나물로 자라는 동안의 아미노산의 변화(變化)를 분석(分析)하였다. 콩나물제조중(製造中) 총(總)아미노산의 변화(變化)를 분석(分析)하였다. 콩나물제조중(製造中) 총(總)아미노산의 변화(變化)는 2일(日)까지는 거의 함량변화(變化)가 없으나 그 이후(以後)부터는 감소(減少)되었고 부위별(部位別)로는 자엽부(子葉部)에서는 감소(減少)되나 배유부(胚乳部)에서는 증가(增加)되었다. 총(總)아미노산에 대(對)한 필수(必修)아미노산의 비(比)는 4일(日) 이후(以後)에 급격(急激)히 감소(減少)되는데 특히 배유부(胚乳部)에서 더 크게 감소(減少)되었다. 여러 아미노산중(中) 특히 aspartic acid의 증가(增加)가 크고 glutamic acid의 감소(減少)가 현저하였다. 총(總)아미노산조성(組成)에 의하여 chemical score를 구(求)하면 A/E와 A/T치(値)는 불규칙(不規則)하게 나타났으나 대두(大豆)와 콩나물 다같이 대체적(大體的)으로 낮은 치(値)를 보였으며 제한(制限)아미노산은 methionin이었다. FAO refernce protein과 requirement pattern을 기준으로하여 구(求)한 chemical score의 값은 2일(日)콩나물>콩>4일(日)콩나물>8일(日)콩나물>6일(日)콩나물의 순(順)으로 나타났고 반면에 essential amino acid index와 requirement index로는 2일(日) 콩나물>콩>4일(日)콩나물>6일(日)콩나물>8일(日)콩나물의 순(順)으로 나타났다.

• KSBB Journal
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• 제4권2호
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• pp.157-161
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• 1989

조기의 유리 아미노산, 총 아미노산의 조성을 규명하기 위해 HPLC를 사용하여 분리정량한 결과와 조지방, 조단백의 결과는 다음과 같다. 1. 조지방 함량은 조기 가식부 : 2.35%, 조기 어란 : 3.65%이고, 조단백질은 조기 가식부 : 46.92%, 조기 어란 : 67.40%로서 다른 어류보다는 조지방은 적으나 조단백질은 비슷하다. 2. 유리 아미노산의 총 함량은 조기 가식부가 323.0mg/100g, 조기 어린이 932.5mg/100g으로 조기 어란이 더 많이 함유되어 있다.

• KSBB Journal
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• 제7권3호
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• pp.186-190
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• 1992

송충이의 유리 아미노산, 총 아미노산 조성과 총 지방산 조성을 규명하기 위해 Gas chromarography와 HPLC를 사용하여 분리 정량한 결과는 다음과 같다. 조지방 함량은 21.00%이고, 조단백질 함량은 58.47%로 조성되어 있다. 유리 아미노산의 총 함량은 3.65g/100g으로 조성되어 있으며, 유리 아미노산 중에서 필수 아미노산의 총 함량은 1.31g/100g으로 35.89%를 차지하고 있다. 총 아미노산의 총 함량은 41.20g/100g으로 조성되어 있으며 총 아미노산 중에서 필수 아미노산의 총 함량은 14.75g/100g으로 35.80%를 차지하고 있다. 총 지방산 조성은 $C_{18}$=26.81%로 제일 많이 함유되어 있고, $C_{16}$=19.09%, $C_{18:1}$=18.74%, $C_{18:3}$=15.33%, $C_{16:1}$=7.29%, $C_{20}$=5.21%순으로 함유되어 있으며, 불포화 지방산이 45.88%이고, 필수 지방산은 19.73%를 차지하고 있다.

• 한국육종학회지
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• 제40권3호
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• pp.269-277
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• 2008

벼 재래종 핵심유전자원의 아미노산조성을 평가하여 벼 품질개량 육종과 기능성 쌀 가공품 개발을 위한 기초소재를 찾기 위한 연구를 수행하였다. 재래종 자원 388품종 간 현미 아미노산 함량의 변이정도는 methionine과 histidine이 매우 컸으며 나머지 아미노산도 변이계수 14.9% 이상의 변이를 보였다. 재래종 핵심유전자원 388품종의 총 아미노산 함량, 제 1제한 그인 lysine함량, 제 2제한 아미노산인 threonine함량 각각의 평균치는 현재 농가에서 재배하고 있는 자포니카형 품종과 비슷하였다. 조사한 재래종 중 백곡,나 대소나, 사두초는 총 아미노산 함량, lysine함량, threonine함량이 월등히 높았고 석산조와 소두조는 아주 낮았다. 또 백곡나, 사두초 및 대소나는 tryptophan을 제외한 7가지 필수아미노산의 총 함량도 아주 높아 이 세 품종은 쌀의 아미노산조성 개량을 위한 유용한 육종소재로 판단하였다. 재래종 품종의 9가지 형태적 특성과 현미 아미노산 함량간의 관계를 검토한 결과 부선색이 자색인 품종은 황백색 품종에 비해 총 아미노산 함량과 serine, proline, glutamic acid, glycine, alanine, valine, leucine, phenylalanine, arginine 함량 모두 적은 쪽에 분포하였으며, aspartic acid는 부선색이 갈색인 품종이 아미노산 함량이 많은 쪽에 분포하였다. 또 주두색이 자색인 품종은 흰색 품종보다 lysine 함량이 많은 쪽에 분포하였다. 그리고 찰벼 품종이 메벼 품종에 비하여 serine, glutamic acid, alanine, valine, leucine 함량은 높은 쪽에 분포하였고, tyrosine 함량은 낮은 쪽에 분포하였다.

• ALGAE
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• 제33권3호
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• pp.279-290
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• 2018

Microalgae have been utilized in diverse industries including aquaculture. Among the microalgae, dinoflagellates are known to have various bioactive compounds, and thus the interest in their application to industry has increased. In order to test their potential as food materials for aquaculture animals, the crude protein contents and compositions of amino acids of six dinoflagellates Heterocapsa rotundata (family Heterocapsaceae), Ansanella granifera (Suessiaceae), Alexandrium andersonii (Ostreopsidaceae), Takayama tasmanica (Brachidiniaceae), Takayama helix, and Gymnodinium smaydae (Gymnodiniaceae) belonging to diverse families were analyzed. The percentage of the amount of the crude protein relative to dry weight of T. tasmanica was the highest (65%) and that of A. andersonii was the lowest (26%). However, the highest percentage of total detected amino acids in crude protein was found in A. andersonii (98.2%). In all six dinoflagellates, glutamic acid was the most dominant amino acid in crude protein. However, the second main amino acid was aspartic acid for H. rotundata, A. granifera, T. helix, and G. smaydae, but were arginine and leucine for A. andersonii and T. tasmanica, respectively. Furthermore, T. tasmanica and T. helix did not have taurine and gamma-aminobutyric acid, whereas the other dinoflagellates possessed them. The percentages of essential amino acid contents of the dinoflagellates met the requirement levels for juvenile shrimps. In addition, the dinoflagellates were not toxic to the brine shrimp Artemia salina. Compared with the other microalgae reported so far, H. rotundata and A. andersonii can be used for arginine-rich diets, T. tasmanica for valine and leucine-rich diets, A. granifera for histidine-rich diets, T. helix for threonine-rich diets, and G. smaydae for lysine-rich diets. Therefore, based on their biochemical composition and toxicity to Artemia, the dinoflagellates could be used as essential amino acid sources for cultivating animals in the aquaculture industry.

• 한국식품영양과학회지
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• 제28권1호
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• pp.178-190
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• 1999

This study was conducted to develop a simulation model for the growth dynamics of pigs and to describe quantitatively protein deposition depending on the amino acid composition of feed protein. In the model it is assumed that the essential processes that determine the utilization of feed protein in the whole body are protein synthesis, breakdown of protein, and oxidation of amino acid. Besides, it is also assumed that occurrence of protein deposition depends on genetic potential and amino acid composition of feed protein. The genetic potential for the protein deposition is the maximum capacity of protein synthesis, being dependent on the protein mass of the whole body. To describe the effect of amino acid composition of feed on the protein deposition, a factor, which consist of ten amino acid functions and lie between 0 and 1, is introduced. Accordingly a model was developed, which is described with 15 flux equations and 11 differential equations and is composed of two compartments. The model describes non linear structure of the protein utilization system of an organism, which is in non steady state. The objective function for the simulation was protein deposition(g/day) cal culated according to the empirical model, PAF(product of amino acid functions) of Menke. The mean of relative difference between the simulated protein deposition and PAF calculated values, lied in a range of 11.8%. The simulated protein synthesis and breakdown rates(g/day) in the whole body showed a parallel behavior in the course of growth.

• 한국식품영양과학회지
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• 제27권2호
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• pp.227-231
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• 1998

To furnish basic data for the utilizatin of camellia(Camellia japonica L.) seeds as a raw material of industrial products, major chemical components of camellia and defatted camellia seeds were investigated. The moisture, crude ash, crude fat and crude protein contents of camellia seeds were 4.06%, 1.83%, 65.75% and 8.44%, respectively. Defatted camellia seeds indicates that it contains 10.31% moisture, 5.28% crude ash and 17.49% crude protein while no crude fat was detected. The curde ginseng saponin, crude saikosaponin and red ginseng saponin contents of camellia seeds were 108.0mg%, 0.4mg% and 40.3mg%, respectively. Total amino acid contents of camellia seeds and defatted camellia seeds were 7,851mg% and 13,002mg%, respectively, and the major amino acids were glutamic acid, arginine, aspartic acid and leucine. The ratio of essential/total amino acid of camellia seeds was 0.29.

• Asian-Australasian Journal of Animal Sciences
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• 제7권4호
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• pp.537-546
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• 1994

This experiment was carried out to compare the effects of six different plant protein sources such as soybean meal, extruded full-fat soybean, canola meal, rapeseed meal, cottonseed meal and perilla meal as a sole protein source of diets on growth performance and amino acid bioavailabilities in growing-finishing pigs. A total of 54 pigs with average 25 kg of body weight were used as experimental subjects for a 65-d feeding trial. Digestion trial was carried out with seven ileal-cannulated pigs. The most rapid rate of weight gain was observed in pigs fed soybean meal and full-fat soybean, the moderate one in pigs fed canola meal and cottonseed meal and the least one in pigs fed rapeseed meal and perilla meal (p<0.005). Feed efficiency was better for groups fed soybean meal and full-fat soybean than other protein meals (p<0.05). The apparent ileal digestibilities of essential amino acids of soybean meal and full-fat soybean (82.5% and 81.6%) were significantly (p<0.05) higher than those of other protein sources (61.2 to 69.4%). Regardless of protein sources, the apparent ileal digestibility of arginine was highest, whereas that of histidine was lowest among essential amino acids. Proline had the lowest digestibility among non-essential amino acids. True amino acid digestibilities tended to be higher than apparent amino acid digestibilities. The differences between true and apparent ileal digestibilities were greater in canola meal, rapeseed meal or cottonseed meal than other protein sources. The differences was greatest in praline except for cottonseed meal. The fecal digestibility appeared to be higher than the ileal digestibility. The differences between fecal and ileal digestibilities were greater in canola meal, rapeseed meal, cottonseed meal and perilla meal than in soybean meal and full-fat soybean. In general, praline was the most disappeared amino acid in the hind gut, while the net synthesis of lysine in the large intestine was observed in all protein sources except perilla meal. It is appropriate that swine feeds should be formulated based on true ileal amino acid digestibility of protein sources for pig's normal growth.

• 한국식생활문화학회지
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• 제23권3호
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• pp.397-402
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• 2008

This study was conducted to evaluate the quality characteristics of domestic as well as imported fermented skate. Three types of fermented skate products were analyzed for proximate composition, pH, VBN, ammonia-N, free amino acids, and fatty acids. The results indicated that the domestic fermented skate contained large amounts of TMAO. Also, the domestic and imported fermented skates each contained approximately 7.1 log CFU/g and $5.8{\sim}6.5$ log CFU/g of aerobic bacteria, respectively, and 585.9 mg and $384.1{\sim}398.5$ mg of total free amino acids, respectively; all samples contained high levels of taurine, anserine, lysine, alanine, glycine, proline, and ${\beta}-alanine$. For fatty acid composition, the domestic fermented skate contained 11 different types of saturated fatty acid and 16 types of unsaturated fatty acid, whereas the imported skate contained 8 types of saturated fatty acid and $10{\sim}15$ types of unsaturated fatty acid. Overall, the results suggest that domestic fermented skate is a better source of amino acids and essential fatty acids and contains more aerobic bacteria than imported fermented skate.

• 한국식품영양과학회지
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• 제23권4호
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• pp.647-653
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• 1994

This study was conducted to investigate the contents of dietary fiber (DF), compositions of fatty acids in lipid fraction and amino acids in salt-soluble protein from the functional food source such as Platycodi radix, perilla seed, evening primrose seed and aloe vera. The contents of dietary fiber, neutral detergent fiber (N.D.F) , acid detergent fiber( A.D.F) . kignin, hemicellulose an dcellulose in evening primorse seed were higher than those of other samples, except the content of cellulose .The ration of polyunsaturated /saturated (P/S) fatty acid in total lipids was 6.31 in perilla seed, which was higher than those of other samples. The content of linolenic acid (n-3) in perilla seed was 55.47%. The content of linoleic acid (n-6) in evening primrose seed was 71.88% , which was higher than those of other samples. The fatty acid composition in neutral lipids were the same as those of total lipids. The PUFA contents of fatty acid in glycolipids were 61.76% in perilla seed. And also, the ratio of n-6/n-3 in evening primrose seed was 15.19. The fatty acid compositions in phospholipids were the sameas those of glycolipids. The contents of PUFA in Platycodi radix were 62.96% . The essential amino acid contents of salt-soluble protein were 47 mole % in Platycodi radix , which was slightly higher than those other samples. The ration of essential amino acid /nonessential amino acid (E/N) was 0.9 and 0.66 in Platycodi radix and aloe vera, respectively.