• Title/Summary/Keyword: Enzyme treatment

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Effect of Myricetin Combined with Taurine on Antioxidant Enzyme System in B16F10 Cell (Myricetin과 Taruine의 병용 투여가 B16F10 세포의 항산화 효소계에 미치는 영향)

  • Yu, Ji-Sun;Kim, An-Keun
    • YAKHAK HOEJI
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    • v.50 no.1
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    • pp.58-63
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    • 2006
  • The antioxidant enzyme (AOE) system plays an important role in the defense against oxidative stress damage. To determine whether myricetin or myricetin/taurine can exert antioxidative effects not only by modulating the AOE system directly but also by scavenging free radical, we investigated the influence of the myricetin and taurine on cell viability ROS level, activities of different antioxidant enzyme, and the expression of different antioxidant enzyme. As results, the cell viability showed inhibition of the proliferation with treatment of 'myricetin' or 'myricetin with taruine', respectively, with dose-dependent manner. Compared to control, the treatment of 'myricetin' decreased activities and gene expressions of superoxide dismutase (SOD), and glutathione peroxidase (GPx). However, combined treatment of 'myricetin with taurine' increased activities and gene expressions of the SOD, GPx, and catalase (CAT). In addition, the combined treatment of 'myricetin with taurine' somewhat decreased ROS levels, compared to the treatment of 'myricetin'. In conclusion, our study provides that the combined treatment of different antioxidants can enhance antioxidant effects.

Advanced Effects of Pulp Bleaching Treated Hemicellulase (Hemicellulase 처리에 의한 목재 펄프의 고해 효과 변화)

  • Kim, Byong-Hyun
    • Journal of the Korean Graphic Arts Communication Society
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    • v.26 no.1
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    • pp.65-72
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    • 2008
  • Kappa number and brightness were more increased with treatment of endo-xylanase than hydrogen-peroxide. In pulp bleaching process, endo-xylanse was most effective in the other enzyme treatment. Exo-xylanase was effective more than 4 unit treatment. Kappa number was tiny increased with enzyme ratio, but less than 4 unit treatment, increased with hydrogen peroxide treatment ratio. In more than 4 unit acetyl-estease treatment, Kappa number and brightness were not influenced with enzyme treatment ratio, but concentration of hydrogen-peroxide.

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β-Glucanase-assisted extraction of starch from glutinous barley (찰보리 전분 추출에 있어서 β-Glucanase 처리 효과)

  • Bae, Jae-Seok;Lee, Eui-Suk;Jeong, Yong-Sun;Kim, Jeong-Won;Lee, Mi-Ja;Hong, Soon-Taek
    • Korean Journal of Agricultural Science
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    • v.39 no.3
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    • pp.387-393
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    • 2012
  • In the present study, ${\beta}$-glucanase-assisted extraction of starch from glutinous barley(Hinchal ssalbory) was investigated. ${\beta}$-glucanase was added to a coarse starch suspension obtained after wet milling in the starch extraction process. It was found that in the isolated starch with enzyme treatment, protein content was lower by 0.03%, compared to that with non-enzyme treatment. More importantly it was observed that the extraction yield of starch from enzyme treatment was found to be about 12% higher than the one from non-enzyme treatment (enzyme treated: 90.56%, non-enzyme treated: 78.46%). In order to elucidate this finding, the mass-balance determination of starch in each extraction step was carried out and found that the enzyme treatment might influence on the insoluble residues(R3 and R4 fractions) to hydrolyze ${\beta}$-glucan and other materials (e.g., mucilages etc.), thereby facilitated the separation of starch from it and a next filtration process. With a phase-contrast microscope it was observed that the isolated starch with enzyme treatment contained small starch granules more than the one with non-enzyme treatment and this might result in higher extraction yield observed with the former. In order to confirm this hypothesis, further experiments would be necessary.

Recycling of Waste Paper with Alkaline Cellulolytic Enzyme(III) - Elimination of sticky contaminants and bleaching of recycling pulp- (호알칼리성 목질 분해 효소를 이용한 페지 재생(제3보) -페지의 이물질 제거 및 표백-)

  • 강석현;이중명;박성배;엄태진
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.36 no.1
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    • pp.30-36
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    • 2004
  • Waste papers(MOW and ONP) were deinked with alkaline cellulolytic enzyme from Coprinus cinereus 2249. The effectiveness of alkaline enzyme on sticky contaminant removal and bleaching of recycling pulp was investigated. The conclusions obtained from the results are as follows. The brightness of deinked pulps MOW and ONP was most high in 0.4IU enzyme treatment. \circled2 The removal effect of sticky contaminant was enhanced with alkaline cellulolytic enzyme treatment. Bleaching with sodium hydrosulfite was most effective in one and two stage bleaching after enzyme treatment of mixed wastepaper of MOW and colored paper. \circled4 The brightness of alkaline enzyme deinked ONP was increased 20% with $H_2O$$_2$ bleaching.

Combined Effects of Pressure Cooking and Enzyme Treatment to Enhance the Digestibility and Physicochemical Properties of Spreadable Liver Sausage

  • Ku, Su-Kyung;Kim, Jake;Kim, Se-Myung;Yong, Hae In;Kim, Bum-Keun;Choi, Yun-Sang
    • Food Science of Animal Resources
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    • v.42 no.3
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    • pp.441-454
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    • 2022
  • This study aimed to determine the effect of enzyme, guar gum, and pressure processing on the digestibility and physicochemical properties of age-friendly liver sausages. Liver sausages were manufactured by adding proteolytic enzyme (Bromelain) and guar gum, and pressure-cooking (0.06 MPa), with the following treatments: control, without proteolytic enzyme; T1, proteolytic enzyme; T2, proteolytic enzyme and guar gum; T3, pressure-cooking; T4, proteolytic enzyme and pressure-cooking; T5, proteolytic enzyme, guar gum, and pressure-cooking. The pH was high in the enzyme- and pressure-processed groups. The pressure-processed groups had lower apparent viscosity than other cooking groups, and it decreased during enzyme treatment. Hardness was lower in the enzyme- and pressure-processed groups than in the control, and the T4 was the lowest. Digestibility was the highest in T4 at 82.58%, and there was no significant difference with that in T5. The general cooking group with enzyme and guar gum also showed higher digestibility than the control (77.50%). As a result of the sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the enzyme- and pressure-treated groups (T4, T5) were degraded more into low-molecular-weight peptides (≤37 kDa) than the control and other treatments. Viscoelasticity showed similar trends for viscous and elastic moduli. Similarly, combined pressure processing and enzymatic treatment decreased viscoelasticity, while guar gum increased elasticity but decreased viscosity. Therefore, the tenderized physical properties and improved digestibility by enzyme and pressurization treatment could be used to produce age-friendly spreadable liver sausages.

The Soy Protein Coagulation Phenomenon by Heat-and Enzyme-Treatment

  • Park, Yang-Won;Kim, Young-Jeon
    • Preventive Nutrition and Food Science
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    • v.2 no.1
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    • pp.77-82
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    • 1997
  • The comparison soy protein coagulation by heat-and enzyme-treatment are summarized. The gelation mechanism of glycinin by heating was mainly due to dissociation and aggregation of the basic subunit of 11S globulin. In case of 7S globulin, macro-soluble aggregates may be formed by noncovalent intraction more than 30min at 8$0^{\circ}C$. Whereas, coagulum occured by the microbial enzyme was more minuter than the other Ca-, HCI-coagulum. Heat treatment attacked the basic subunit of 11S globulin and this results agreed very, how-ever, preferred acidic subunit to basic subunit of 11S globulin and attacked the 7S globulin, that could produce coagulum products within 4~5min at $65^{\circ}C$.

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Strength Restoration of The DP Finished Cotton Fabric by Enzymatic Treatment (수지 가공 면직물의 강도 회복을 위한 효소처리 연구)

  • 전미선;김주혜;박명자
    • The Research Journal of the Costume Culture
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    • v.12 no.5
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    • pp.737-742
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    • 2004
  • The purpose of this study is restoration for tearing strength of the durable press (DP) finished 100% cotton fabric by enzymatic treatment. Dimethylol Dihydroxy Ethylene Urea (DMDHEU) was used as a DP finish chemical. Enzymes (cellulase, pectinase, protease, lipolase) were selected based on their specific reaction activities. Ideal application of the enzymes for this work was to remove cross-links created by DMDHEU on the surface of the fibers to offer migration property between microstructures of cellulose, yet cross-links that exist inside of the fibers are still remained to impart effect of wrinkle resistance. Physical characteristics (tearing strength, wrinkle recovery, FT-IR) of enzyme treated samples were measured and compared. It was found out that, in case of enzyme treatment, most of enzymes didn't have a great effect on tearing strength, but, in case of Protease, tearing strength increased at DMDHEU 2% treatment. As a result of an experiment on wrinkle recovery of the textiles treated with enzyme making density of DMDHEU different whenever respective experiment was made, it was discovered that density of DMDHEU increased as wrinkle recovery increased and, in the relation to enzyme treatment especially in Lipase enzyme treatment, the lesser density of DMDHEU, the more wrinkle recovery increased.

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Quality Properties and Carotenoid Pigments of Yellow Sweet Potato Puree (황색고구마 퓨레의 품질과 Carotenoid색소)

  • 정순택;임종환;강성국
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.4
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    • pp.596-602
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    • 1998
  • Two kinds of sweet potato puree were prepared with Benihayato cultivar of yellow sweet potato with or without $\alpha$-amylase enzyme treatment. Chemical and rheological properties of enzyme-treated puree were different from those of control puree. Reducing sugar content and iodine value increased by $\alpha$-amylase enzyme treatment, while alcohol insoluble solids and viscosity decreased by enzyme treatment. However, the changes of carotenoid content were not significantly different. Hunter-b-values(yellowness) were 27.19 and 23.54 for no enzyme-treated puree(NTP) and enzyme-treated puree(ETP), respectively. Hunter-a values(redness) were 2.24 and 6.05 for NTP abd ETP, respectively. Content of total carotenoid of canned puree heated at 13$0^{\circ}C$ for 30 min decreased by 59 percents.

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Effect of Jebikong(Dolichos lablab L.) Leaves Extract on Enzyme Activities in Mice Blood (제비콩(Dolichos lablab L.) 잎 추출물이 쥐의 혈청효소활성에 미치는 영향)

  • 김용욱;최우철;신미경;한성희;임세진;김명애
    • Korean Journal of Organic Agriculture
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    • v.9 no.3
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    • pp.85-92
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    • 2001
  • The effect of Dolichos lablab leave extract on enzyme activities in mice blood was investigated in this study to clarify the new useful application of the Plant leaves. There were not significant differences in the enzyme activities in mice blood among treatment fed with the leave extract and non fed control. The feeding treatment with the extract showed a tendency to activity compared to the nun fed control. The activities of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase and lactate dehydrogenase in mice blood at the treatment fed with lead and the leave extract were significantly low compared to their activities at the non fed treatment with the extract, respectively. The choline esterase activity was high at the leave extract feeding. The cadmium dietary treatment showed the same result as the lead treatment. In conclusion, the physiological function of the Jebikong leave was significantly in creased when the mice was stressed by the hear metal intake. Therefore, the plant leave extract would consider the reduction of heart metal effect.

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Effect of Phytase, Protease and the Mixed Enzyme of Phytase and Protease on the Extraction and Properteis of the Protein from Abolished Soybean Meal (Phytase, Protease 및 Phytase와 Protease 혼합 효소처리가 폐대두박의 단백질 추출율 및 그 기능성에 미치는 영향)

  • 조영제;천성숙
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.1
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    • pp.57-63
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    • 2000
  • To extract insoluble proteins from abolished soybean meal, the meal was treatesd with phytase and protease produced by Aspergillus sp. SM-15 and Aspergillus sp. MS-18. The extraction of insoluble soybean protein was increased at alkaline range more than pH 5 in case of phytase, pH 7 to 11 in case of protease and pH 5 to 12 in case of the mixed enzyme of phytase and protease. The optimum extraction temperature of insoluble protein was 5$0^{\circ}C$ for phytase and the mixed enzyme of phytase and protease, and 6$0^{\circ}C$ for protease. The optimum treatment time for extraction of protein was 9 hrs for phytase, 11 hrs for protease and the mixed enzyme of phytase and protease and optimum unit of enzyme for extraction of protein was 600 unit, 40 unit and 900 unit+60 unit in case of phytase, protease, phytase and protease, respectively. The treatment of mixed enzyme showed higher extracton rate of protein than single enzyme treatment. The foaming capacity, foaming stability, emulsion capacity, and emulsion stability of soybean meal protein by the treatment of the enzymes increased at all pH range. Further more oil absorption as well as water absorption capacities by the treatment of the enzymes were also increased. The functional properteis of the soybean meal protein treated by the mixed enzyme were higher than those of soybean meal protein treated by the single enzyme.

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