• The Plant Pathology Journal
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• 제38권4호
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• pp.417-422
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• 2022

Apple stem grooving virus (ASGV) is a destructive viral pathogen of pome fruit trees that causes significant losses to fruit production worldwide. Obtaining ASGV-free propagation materials is essential to reduce economic losses, and accurate and sensitive detection methods to screen ASGV-free plantlets during in vitro propagation are urgently necessary. In this study, ASGV was sensitively and accurately quantified from in vitro propagated apple plantlets using a reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay. The optimized RT-ddPCR assay was specific to other apple viruses, and was at least 10-times more sensitive than RT-real-time quantitative PCR assay. Furthermore, the optimized RT-ddPCR assay was validated for the detection and quantification of ASGV using micropropagated apple plantlet samples. This RT-ddPCR assay can be utilized for the accurate quantitative detection of ASGV infection in ASGV-free certification programs, and can thus contribute to the production of ASGV-free apple trees.

• 한국조경학회지
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• 제34권4호
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• pp.84-95
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• 2006

This study begins by examining the reason for the lack of urban planning that takes the water cycle into consideration. While there are institutions that support environmentally friendly development or smooth water circulation, these designs are not reflected in planning nor in the real world. After reviewing foreign case studies, policy suggestions and possible policy implications for Korea are derived. In Korea, there is not a sufficient level of relevant laws or institutions systematically established to make it possible to deal with rainwater in a decentralized way. Instead, facility standards or guidelines are considered separately for the control of water and for preventing natural disasters. And even though an environmentally friendly approach is stipulated in relevant laws in terms of spatial planning, there are no planning systems or implementation tools to actualize this kind of approach. The factors that make decentralized rainwater management possible in urban planning are analyzed based on the case study of Germany. Germany requires developers to plan in order to achieve ecological urban development. In addition, as a detailed implementation tool to promote conservation of the water cycle, the law provides for various kinds of measures such as restrictions on the proportion of impervious surface area according to the use of the land, required compensation measures for environmental degradation following development, introduction of a fee for rainwater runoff and the establishment of ecological landscape planning. The actual reason these measures can be implemented however is the provision of planning guidelines and design criteria for rainwater utilization, absorption and containment, and the construction of a database for various environmental information.

• 기술경영경제학회:학술대회논문집
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• 기술경영경제학회 2005년도 제26회 동계학술발표회 논문집
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• pp.109-123
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• 2005

There has been great effect on development and usage of resources as well as technological development including everyday life pattern to maintain environmental sustainability, Also, It is tend to become negative for people's concern of nature ecosystem toward new technological developments because it is neither environmentally, nor naturally friendly. Therefore, our study is to implement expert education and research system which includes the trends and requests of our society with objectives as follows; First, Training industrial technology and policy maker who can understand and practice ecological ethics. Second, Educating people with expertise which can evaluate and complement the environmental friendliness about new industrial technologies. Third, Educating people who can develop technology which is environmentally friendly. Our new program requires various knowledges and balanced point of view. To this end, students are required to understand field of art and sciences including emerging eco-buddhism subject as well as field of state-of-the art environmental technologies such as recycling technology and clean production technology.

• 한국환경농학회지
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• 제30권3호
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• pp.323-329
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• 2011

BACKGROUND: The conazoles, difenoconazole, diniconazole, hexaconazole, penconazole and tetraconazole are a large class of synthetic fungicides used extensively for foliage and seed treatments in agricultural crops. The extensive use of conazoles has brought concerns on the potentiality of environmental contamination and toxicity. Thus studies on the development of methods for monitoring the conazoles are required. METHODS AND RESULTS: A modified quick, easy, effective, rugged and safe (QuEChERS) method was involved in sample preparation. Quadrapole time of flight mass spectrometer (Q-TOF MS) in electron spray ionization (ESI) mode was employed to determine conazoles in garlic samples. The limit of detection (LOD) and limit of quantification (LOQ) of conazoles by Q-TOF-MS ranged from 0.001 to 0.002 mg/L and 0.002 to 0.005 mg/L, respectively. Q-TOF-MS analysis exhibited less than 2.6 ppm error of accurate mass measurements for the detection of conazoles spiked at 0.05 mg/L in garlic matrix. Recovery values of conazoles fortified in garlic samples at 0.02, 0.05 and 0.1 mg/L were between 79.2 and 106.2% with a maximum 11.8% of standard deviation. No detectable conazoles were found in the domestic market samples by using the Q-TOF-MS method. CONCLUSION(s): High degree of confirmation for conazoles by accurate mass measurements demonstrated that Q-TOF-MS analysis combined with a QuEChERS method may be applicable to simultaneous determination of conazoles in garlic samples.

• 한국토양비료학회지
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• 제47권4호
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• pp.299-305
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• 2014

In this paper, fractional factorial screening design (FFSD) and central composition design (CCD) were used to optimize the medium components for producing chitinase and gelatinase by Lysobacter capsici YS1215. Crab shell powder, nutrient broth and gelatin were proved to have significant effects on chitinase and gelatinase activity by FFSD first. An optimal medium was obtained by using a three factor CCD, which consisted of nutrient broth of $2.0gL^{-1}$, crab shell powder of $2.0gL^{-1}$ and gelatin of $1.0gL^{-1}$, respectively with the highest chitinase activity ($3.34UmL^{-1}$) and gelatinase activity ($14.15UmL^{-1}$). This value was 3.76 and 1.11 fold of the chitinase and gelatinase activity, respectively, compared to the lowest productive medium in the design matrix. In investigating potential of these enzymes partially purified from L. capsici YS1215 for biotechnological use, the crude enzymes was found to be inhibition against pathogenic fungal mycelia: Colletotrichum gleosporioides, Phytophthora capsici, and Rhizoctonia solani. In this study, we demonstrated the optimal medium for producing the chitinolytic and gelatinolytic enzymes by the strain YS1215 and the role of their enzymes that may be useful for further development of a biotechnological use and agricultural use for biological control of phytopathogenic fungi.

• Journal of Microbiology and Biotechnology
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• 제27권10호
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• pp.1763-1772
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• 2017

This study aimed to isolate and characterize antibacterial metabolites from Pharbitis nil seeds and investigate their antibacterial activity against various plant pathogenic bacteria. The methanol extract of P. nil seeds showed the strongest activity against Xanthomonas arboricola pv. pruni (Xap) with a minimum inhibition concentration (MIC) value of $250{\mu}g/ml$. Among the three solvent layers obtained from the methanol extract of P. nil seeds, only the butanol layer displayed the activity with an MIC value of $125{\mu}g/ml$ against Xap. An antibacterial fraction was obtained from P. nil seeds by repeated column chromatography and identified as pharbitin, a crude resin glycoside, by instrumental analysis. The antibacterial activity of pharbitin was tested in vitro against 14 phytopathogenic bacteria, and it was found to inhibit Ralstonia solanacearum and four Xanthomonas species. The minimum inhibitory concentration values against the five bacteria were $125-500{\mu}g/ml$ for the n-butanol layer and $31.25-125{\mu}g/ml$ for pharbitin. In a detached peach leaf assay, it effectively suppressed the development of bacterial leaf spot, with a control value of 87.5% at $500{\mu}g/ml$. In addition, pharbitin strongly reduced the development of bacterial wilt on tomato seedlings by 97.4% at $250{\mu}g/ml$, 7 days after inoculation. These findings suggest that the crude extract of P. nil seeds can be used as an alternative biopesticide for the control of plant diseases caused by R. solanacearum and Xanthomonas spp. This is the first report on the antibacterial activity of pharbitin against phytopathogenic bacteria.

• 한국유기농업학회지
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• 제28권1호
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• pp.69-93
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• 2020

The paper is to compare two villages in Songak-myeon, Asan, Cnungnam Province in Korea. It tries to show how the joint village project, which began with the support of the same external organization, produces different results depending on the internal conditions and circumstances of the village. From the end of the 1990s, Pyeongchon-ri and Sugok-ri started developing villages under the influence of the environmentally friendly farming movement. And subsequent supports were provided by local organizations for the development of the village. However, the village project in Sugok-ri, which focused on economic performance, later turned out to be a failure, and the residents changed to pursue activities for their own economic interests individually. On the other hand, In Pyeongchon-ri, the cooperation among villagers was strengthened in implementing support projects by similar external agencies. In addition, community ties and identity have been strengthened as problems have been solved through cooperation among residents in the process of promoting the village project. Nevertheless, projects undertaken in Pyeongchon-ri for the purpose of increasing economic performance was all failed. Through these findings, the paper asserts that the neo-endogenous development approach utilizing external support on the basis of internal cooperative network among villagers must be a very useful method for rural development in contemporary societies.

• 한국실내디자인학회:학술대회논문집
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• 한국실내디자인학회 2006년도 춘계학술발표대회 논문집
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• pp.154-157
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• 2006

With the sustainable development theory introduced, some planning factors, such as how to minimize bad effects on the environment and how to be more environmentally friendly, have been Integrated in planning the residential development. In addition, a new residential space concept, the ubiquitous home, arouse the interest of architects and residents. Although there have been many efforts to achieve the environment-friendly and convenient residence, the problem is that the environment-friendly architecture's many of the efforts have been primarily focused on the outside of the apartment units. Thus, the purpose of this study is to show possibilities of housing model not only being applied the environment-friendly construction concept to the inside of the apartment units, but also being integrated the ubiquitous home model inside.

• 월간포장계
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• 통권312호
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• pp.61-65
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• 2019

• 한국환경복원기술학회지
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• 제2권3호
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• pp.74-82
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• 1999

This study is aimed to establish the development plannings for the sustainable tourism of Yongyoo Island and Mooeui Island, neighboring areas of Youngjong Island, for the conservation and environmentally friendly use of Korean coastal wetlands. The concepts of sustainable tourism is described. The current development status and its problems of Youngjong Island and its neighboring areas are analysed. The development plannings for sustainable tourism of Yongyoo Island and Mooeui Island are suggested as follows : (1) The small scale tourism development projects, which are able to represent region's own characteristics, instead of large scale ones, should be performed. (2) The participation of community people should be guaranteed at the beginning stage of the decision making processes for tourism development projects. Additionally, the profits of the development should be fairly distributed among community people. (3) The coastal wetlands should be conserved and their sewage disposal capacity should be protected by way of the minimization of reclamation. (4) The coastal wetlands should be developed into the tourist resorts utilizing migratory birds as tourism resources. In addition, the carrying capacity of the coastal wetlands, which is based on the current status of ecosystems and the Environmental Impact Assessment(EIA), should be calculated.