• Korean Journal of Environmental Agriculture
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• v.36 no.2
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• pp.106-112
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• 2017

BACKGROUND: Kale (Brassica oleracea) biosynthesizes various phytochemicals including glucosinolates, flavonoids, and carotenoids. Phytochemicals of plants are influenced by light, temperature, carbon dioxide, and growing conditions. Specifically, carotenoids are affected by temperature, light, and oxygen. The aim of this study was to investigate the effect of cold stress (day/night: $25^{\circ}C/20^{\circ}C$, $20^{\circ}C/15^{\circ}C$, $15^{\circ}C/10^{\circ}C$) on carotenoids in kale leaves. METHODS AND RESULTS: Kale was grown in pots for up to 50 days after sowing (DAS) in a greenhouse. For cold acclimation experiments, kale grown in growth chambers for 3 days and was subjected to low temperature for 4 days. The conditions maintained in the growth chambers were as follows: photoperiod, 12/12 h (day/night); light, fluorescent; and relative humidity, 60%. Carotenoid (lutein, ${\alpha}-carotene$, zeaxanthin, ${\beta}-carotene$) contents were analyzed by high-performance liquid chromatography (HPLC). The total carotenoid content gradually increased during cold acclimation for 3 days. When kale was subjected to cold stress, the total carotenoid content was high at $25^{\circ}C/20^{\circ}C$ treatment, but low at $15^{\circ}C/10^{\circ}C$ treatment. The total carotenoid content of kale leaves continuously grown in greenhouse decreased from 50 to 57 DAS (1,418 and 1,160 mgkg-1 dry wt., respectively). The lutein, ${\alpha}-carotene$, and ${\beta}-carotene$ contents were very low and the zeaxanthin contents were very high at $15^{\circ}C/10^{\circ}C$ treatment. When kale was subjected to cold stress, the ratio of individual to the total carotenoid contents of kale leaves was 4553% for -carotene and 210% for zeaxanthin. CONCLUSION: The ${\beta}-carotene$ and zeaxanthin contents in kale leaves indicate their sensitiveness toward cold stress.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.6
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• pp.322-330
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• 2003

The unicellular green alga Haematococcus pluvialis has recently attracted great inter-est due to its large amounts of ketocarotenoid astaxanthin, 3,3'-dihydroxy-${\beta}$,${\beta}$-carotene-4,4'-dione, widely used commercially as a source of pigment for aquaculture. In the life cycle of H. pluvialis, astaxanthin biosynthesis is associated with a remarkable morphological change from green motile vegetative cells into red immotile cyst cells as the resting stage. In recent years we have studied this morphological process from two aspects: defining conditions governing astaxanthin biosynthesis and questioning the possible function of astaxanthin in protecting algal cells against environmental stress. Astaxanthin accumulation in cysts was induced by a variety of environmental conditions of oxidative stress caused by reactive oxygen species, intense light, drought, high salinity, and high temperature. In the adaptation to stress, abscisic acid induced by reactive oxygen species, would function as a hormone in algal morphogenesis from veget ative to cyst cells. Furthermore, measurements of both in vitro and in vivo antioxidative activities of astaxanthin clearly demonstrated that tolerance to excessive reactive oxygen species is greater in astaxanthin-rich cysts than in astaxanthin-poor cysts or astaxanthin-less vegetative cells. Therefore, reactive oxygen species are involved in the regulation of both algal morph O-genesis and carotenogenesis, and the accumulated astaxanthin in cysts can function as a protective agent against oxidative stress damage. In this study, the physiological roles of astaxanthin in stress response and cell protection are reviewed.

• Computers and Concrete
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• v.3 no.1
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• pp.43-64
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• 2006

A tendon model that can effectively be used in finite element analyses of prestressed concrete (PSC) structures with bonded tendons is proposed on the basis of the bond characteristics between a tendon and its surrounding concrete. Since tensile forces between adjacent cracks are transmitted from a tendon to concrete by bond forces, the constitutive law of a bonded tendon stiffened by grouting is different from that of a bare tendon. Accordingly, the apparent yield stress of an embedded tendon is determined from the bond-slip relationship. The definition of the multi-linear average stress-strain relationship is then obtained through a linear interpolation of the stress difference at the post-yielding stage. Unlike in the case of a bonded tendon, on the other hand, a stress increase beyond the effective prestress in an unbonded tendon is not section-dependent but member-dependent. The tendon stress unequivocally represents a uniform distribution along the length when the friction loss is excluded. Thus, using a strain reduction factor, the modified stress-strain curve of an unbonded tendon is derived by successive iterations. The validity of the proposed two tendon models is verified through correlation studies between analytical and experimental results for PSC beams and slabs.

• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.1039-1046
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• 2005

After clay particles have been sediment isotropically, the clay deposits have been consolidated under $K_0$-stress system. Therefore, in order to predict the behavior in-situ of normally consolidated clays, the laboratory test should be enforced under $K_0$-stress system and should obtain the characteristics of normally consolidated clays. And relationship of stress-strain on clay is effected on not only method of consolidation but also characteristic of visco-plastic behavior. Saturated clay is effected more this trend. So, rate of strain is considered to understand exact stress-strain relationship. In this study, the series of undrained triaxial compression tests were preformed on remolded specimens which was made by slurry of clay, consolidated under $K_0$-stress systems. And the undrained triaxial compression test were preformed to examine behavior of stress-strain relationship due to rate of shear strain relationship due to rate of shear strain.

• Journal of Navigation and Port Research
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• v.33 no.10
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• pp.665-669
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• 2009

When maneuvering a ship in a narrow channel or under bridge, the ship operator may take actions of slowdown engine and altering course in order to avoid possible navigational dangers, which may reduce difficulties on navigation or collsion avoidance against other ship and/or bridge. In this paper, taking notice of the stress caused by these actions of slowdown engine and altering course, survey is carried out for whether it is possible to quantify the stress by time delay as an index. Based on the ship handling simulator experiment, it is verified that difficulty of navigation changes is highly correlated with the time delay, which result from compensation actions of slowdown engine and altering course.

• Journal of Korean Society on Water Environment
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• v.25 no.4
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• pp.507-514
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• 2009

In this study, a dynamic modeling scheme is presented to describe the probabilistic structure of soil water and plant water stress index under stochastic precipitation conditions. The proposed model has the form of the Fokker-Planck equation, and its applicability as a model for the probabilistic evolution of the soil water and plant water stress index is investigated under a climate change scenario. The simulation results of soil water confirm that the proposed soil water model can properly reproduce the observations and show that the soil water behaves with consistent cycle based on the precipitation pattern. The simulation results of plant water stress index show two different PDF patterns according to the precipitation. The simple impact assessment of climate change to soil water and plant water stress is discussed with Korean Meteorological Administration regional climate model.

• Journal of Crop Science and Biotechnology
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• v.10 no.1
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• pp.39-44
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• 2007

The effect of inabenfide was investigated in rice seedlings subjected to salt stress in relation to changes in chlorophyll fluorescence(${\Delta}F/Fm'$), lipid peroxidation, salicylic acid(SA) content, and catalase(CAT) activity. A reduction in shoot growth of rice seedlings by 120 mM NaCl treatment was significantly alleviated by pretreatment with 30 ${\mu}M$ inabenfide. Sodium ion content was not affected by pretreatment with inabenfide, suggesting that alleviation was not due to a reduction in sodium ion uptake by the rice seedlings. At three days after NaCl treatment, the rice seedlings pretreated with inabenfide showed a higher ${\Delta}F/Fm'$(30%) and lower lipid peroxidation(28%) compared with the rice seedlings treated with NaCl alone. After NaCl treatment, CAT activity in the third leaf of rice seedlings decreased significantly but alleviated by pretreatment with inabenfide. Furthermore, pretreatment with inabenfide also reduced the level of SA which accumulated drastically in the third leaf of rice seedlings within a day after exposure to salt stress. These results suggest that inabenfide prevents SA accumulation in rice seedlings under salt stress which eventually induces the alleviation of salt stress damage.

• Mycobiology
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• v.37 no.3
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• pp.161-170
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• 2009

Cryptococcus neoformans is a basidiomycete human fungal pathogen that causes meningoencephalitis in both immunocompromised and immunocompetent individuals. The ability to sense and respond to diverse extracellular signals is essential for the pathogen to infect and cause disease in the host. Four major stress-activated signaling (SAS) pathways have been characterized in C. neoformans, including the HOG (high osmolarity glycerol response), PKC/Mpk1 MAPK (mitogen-activated protein kinase), calcium-dependent calcineurin, and RAS signaling pathways. The HOG pathway in C. neoformans not only controls responses to diverse environmental stresses, including osmotic shock, UV irradiation, oxidative stress, heavy metal stress, antifungal drugs, toxic metabolites, and high temperature, but also regulates ergosterol biosynthesis. The PKC(protein kinase C)/Mpk1 pathway in C. neoformans is involved in a variety of stress responses, including osmotic, oxidative, and nitrosative stresses and breaches of cell wall integrity. The $Ca^{2+}$/calmodulin- and Ras-signaling pathways also play critical roles in adaptation to certain environmental stresses, such as high temperature and sexual differentiation. Perturbation of the SAS pathways not only impairs the ability of C. neoformans to resist a variety of environmental stresses during host infection, but also affects production of virulence factors, such as capsule and melanin. A drug(s) capable of targeting signaling components of the SAS pathway will be effective for treatment of cryptococcosis.

• Computers and Concrete
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• v.11 no.2
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• pp.169-182
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• 2013

One of the major benefits of the pre-stressed concrete pavements is the omission of tension in concrete that results in a reduction of cracks in the concrete slabs. Therefore, the life of the pavement is increased as the thickness of the slabs is reduced. One of the most important issues in dealing with the prestressed concrete pavement is determination of the magnitude of the pre-stress. Three dimensional finite element analyses are conducted in this research to study the pre-stress under various load (Boeing 777) and thermal gradient combinations. The model was also analyzed under temperature gradients without the presence of traffic loading and the induced stresses were compared with those from theoretical relationships. It was seen that the theoretical relationships result in conservative values for the stress.

• Journal of Environmental Science International
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• v.21 no.5
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• pp.605-611
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• 2012

The purpose of this study is to analyze psychological and physiological effects accordance with viewing and walking in the forest and urban area. In the result of measurement of physiological reactions in nervous system, viewing of the forest had a calming effect on the nervous system by reducing blood pressure and heart rate. The other hand, viewing and walking in the urban area compared to the forest area raised stress by increasing blood pressure and heart rate. In addition, viewing in forest area was effective in stress relief by noticeable reduction of the amylase concentration. In contrast, walking in the urban area was also confirmed an increase of stress by increasing the concentration of the amylase. A viewing and walking in forest area was effective in alleviating depressed on anxiety, anger, fatigue and confusion.