• Nuclear Engineering and Technology
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• 제53권4호
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• pp.1210-1217
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• 2021

The analysis of ⁴¹Ca in concrete generated from the nuclear facilities decommissioning is critical for ensuring the safe management of radioactive waste. An analytical method for the determination of ⁴¹Ca in concrete is described. ⁴¹Ca is a neutron-activated long radionuclide, and hence, for accurate analysis, it is necessary to completely extract Ca from the concrete sample where it exists as the predominant element. The decomposition methods employed were the acid leaching, microwave digestion, and alkali fusion. A comparison of the results indicated that the alkali fusion is the most suitable way for the separation of Ca from the concrete sample. Several processes of hydroxide and carbonate precipitation were employed to separate ⁴¹Ca from interferences. The method relies on the differences in the solubility of the generated products. The behavior of Ca and the interfering elements such as Fe, Ni, Co, Eu, Ba, and Sr is examined at each separation step. The purified ⁴¹Ca was measured by a liquid scintillation counter, and the quench curve and counting efficiency were determined by using a certified reference material of known ⁴¹Ca activity. The recoveries in this study ranged from 56 to 68%, and the minimum detectable activity was 50 mBq g^-1 with 0.5 g of concrete sample.

• 한국환경보건학회지
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• 제40권1호
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• pp.55-62
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• 2014

Objectives: $^{32}P$-postlabeling assay is the most sensitive method of detecting DNA adduct formation. However, it is limited by a low sample throughput and use of radioisotopes (RI). In this study, we modified it to minimize these limitations and applied it to Z. platypus exposed to Benzo(a)pyrene (BaP) in order to investigate DNA adduct formation (effect biomarker for pollutants) in Z. platypus for assessing risk of waterborne BaP exposure. Methods: DNA hydrolysis was performed only with Micrococcal nuclease (MNase), RI reduction test was performed and the overlapping steps between thin layer chromatography (TLC) and radioisotope high-performance liquid chromatography (RI-HPLC) were omitted. The application of a modified method to Z. platypus exposed to BaP was performed. Results: The results revealed that the amount of RIs used can be reduced roughly 10-fold. Because the analysis time was shortened by 8.5 hours, the sample throughput per hour was increased compared with the previous method. The results of applying modified $^{32}P$-postlabeling assay to Z. platypus, DNA adduct formation in Z. platypus showed dose-dependency with the BaP concentration. Only BPDE-dGMP was detected as a DNA adduct. Conclusion: These results demonstrate that the modified $^{32}P$-postlabeling assay is a suitable method for detecting DNA adduct formation in Z. platypus exposed to waterborne BaP and will be useful in risk assessment of carcinogenic effect in aquatic environment due to BaP.

• 한국환경과학회지
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• 제10권5호
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• pp.373-380
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• 2001

In recent years, the demand of activated carbon has been increasing steadily because of the environmental problems. Among them waste and water treatment and removal of poisonous gas were invorved. Therefore, in this study, activated carbon was made from the waste chinese cabbage and measured the iodine adsorption ability, carbonization yield, and activation yield of the produced activated carbon. The result showed that the carbonization yield was decreased when carbonization temperature was increaed and that the optimal carbonization temperature was $600{\circ}C$. The optimal concentration of NaOH for removing ash in the raw sample was 1~2N. The range of iodine adsorption number of activated carbon using chinese cabbage at $600{\circ}C$. carbonization was 610.82mg/g to 1019.58mg/g. The activation result of carbonization sample showed that the optimal activation condition was the carbonization at $400\circ}C$ and the activation at$700{\circ}C$. So the production of activated carbon using chinese cabbage was possible in the aspect of reuse of resource and decrease of environmental pollution compared to the commercial activated carbon.

• 한국대기환경학회지
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• 제28권2호
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• pp.182-189
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• 2012

Co-combustion of sludge with coal is proposed as an alternative option for sludge disposal instead of ocean dumping. Because the combustion of sludge can cause emissions of various air pollutants, it is important to understand the characteristics of sludge combustion flue gases. Especially, very few studies are found to investigate mercury emissions from the co-combustion of sludge with coal. In this study, coal and sludge samples were prepared with different mixing ratios. These samples were combusted in a bench-scale combustor, and their flue gases were analyzed. Due to higher mercury content in the sludge sample, higher mercury emissions were found with an increase in a ratio of sludge to coal. Compared to the combustion flue gas of the coal sample, higher levels of mercury oxidation were found in the combustion flue gas of the samples including sludge.

• 한국환경농학회지
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• 제29권3호
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• pp.247-256
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• 2010

A high-performance liquid chromatographic (HPLC) method was developed to determine buprofezin residues in hulled rice and fruits. The buprofezin residue was extracted with acetone and the extract was stepwise purified by liquid-liquid partition and Florisil column chromatography. For rice samples, acetonitrile/n-hexane partition was additionally employed to remove nonpolar lipids. Reversed phase HPLC using an octadecylsilyl column was successfully applied to separate buprofezin from sample co-extractives, as detected by ultraviolet absorption at 250 nm. Recovery experiment at the limit of quantitation validated that the proposed method could evidently determine the buprofezin residue at the level of 0.02 mg/kg. Mean recoveries from hulled rice, apple, pear, and persimmon samples fortified at three tenfold levels were in the range of 80.8~85.2%, 89.1~98.4%, 88.8~95.7% and 90.8~96.2%, respectively. Relative standard deviations of the analytical method were all less than 5%, irrespective of sample types. A selected-ion monitoring LC/mass spectrometry with positive electrospray ionization was also provided to sensitively confirm the suspected residue.

• 한국물환경학회지
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• 제23권1호
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• pp.12-18
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• 2007

Difference of total coliforms (TC) survival time of mixed samples (effluent were mixed by seawater or freshwater) were examined in various conditions. The time taken to achieve a 90% reduction in the TC concentration ($T_{90}$) of effluent, high initial TC concentration and low initial TC concentration samples indicated 143.9, 121.9 and 89.6 hours at $25^{\circ}C$, respectively. At $4^{\circ}C$, log removal rate after 336 hours were 0.96, 1.04, and 1.30. TC survival time of effluent-inoculated seawater sample was longer than that of effluent-inoculated freshwater sample in laboratory. At outdoor condition, TC inactivation curves of effluent-inoculated seawater or freshwater samples showed similar patterns. And both of them were greatly influenced by climate condition. There was not enough evidence that TC survived longer in freshwater than sea water. It was unlikely that the salinity of sea water contributed to the inactivation process of TC.

• 환경생물
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• 제20권4호
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• pp.294-299
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• 2002

PM (Particulate Matter) samples contained fungal spores were collected in the ambient air of Seosan, west Korea, in springtime of 2000 and 2001. PM concentrations were $199.8\mu{g}\;m^{-3}$ in the 1st Asian Dust Storm period (March, 23 ~ 24) and $249.4\mu{g}\;m^{-3}$ in the 2nd period (April, 7~9), 2000. Compared with the concentrations in 2000, relatively low PM concentration ($157.3\mu{g}\;m^{-3}$ ) was measured in the Asian Dust Storm period (April, 24 ~ 20) of 2001. Although there were somewhat differences for the total PM concentrations among the three periods, majorities of the PM were composed of coarse particles sized about 5 ~ 6 $\mu{m}$ over the periods of the two years. Diverse molds grown from fungal aerosols were observed in the PM samples and identified at the genus level. All the genera, Fusarium, Aspergillus, Penicillium, Basipetospora, Epicoccum and Monotospora are hyphomycetes in the division Fungi imperfecti (Deuteromycota). Especially, morphologically more diversified mycelia of hyphomycetes were grown on the fine PM sample (1.1 ~ 2.1 $\mu{m}$) than coarse PM samples in the periods of 2000. Furthermore, some molds were grown on even the background PM sample less than 0.43 $\mu{m}$ in the period of 2001. It was thought that some kinds of ultra fine sized fungal spores were transported by the Asian Dust Storm and suspended in the ambient air of study area during the events.

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2001년도 Proceedings of the 3rd Annual Meeting of Yellow Sea Environment
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• pp.1.1-5
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• 2001

High SPM concentrations(199.8~249.4${\mu}{\textrm}{m}$/㎥) were detected in the west Korea during the Yellow Sand Periods, 2000. Majority of the total SPM were composed of about 5${\mu}{\textrm}{m}$ sized coarse particles over the periods. However, fine particles sized about 1 ${\mu}{\textrm}{m}$ and coarse particles sized about 5-6${\mu}{\textrm}{m}$ showed peaks at the graph of SPM size distribution in the Non Yellow Sand Period. Airborne fungal spores at the SPM samples were cultured and identified. Full-grown colonies during the Yellow Sand Periods, Fusarium, Aspergillus, Penicillium and Basipetospora are hyphomycetes in the division Fungi imperfecti(Deuteromycota). And morphologically more diversified mycelia of hyphomycetes were grown on the sample captured from 1.1~2.1${\mu}{\textrm}{m}$ sized SPM than on other sized samples during the Yellow Sand Period. But no mold was observed on the sample of 1.1~2.1${\mu}{\textrm}{m}$ sized SPM in the Non Yellow Sand Period. It was thought that several sorts of fine sized fungal spores were suspended in the atmospheric environment of the west Korea during the Asian dust episodes.

• 수산경영론집
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• 제35권1호
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• pp.117-132
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• 2004

This study attempts to suggest a new approach of the estimation of range and degree of fisheries damages caused by a large scale of reclamation undertaken in coastal area using the central limit theorem(CLT) in statistics. The key result of the study is the introduction of the new concept of critical variation of environmental factor($d_{c}$). The study defines $d_{c}$ as a standard deviation of the sample mean($\bar{X}$) of environmental factor(X), in other words, $\frac{\sigma}{ \sqrt{n}}$. The inner bound of $d_{c}$ could be the area of fisheries damages caused by public coastal undertaking. The study also defines the decreasing rate of fisheries production$\delta_{\varepsilon}$, in other words, degree of fisheries damages, as the rate of change in the distribution of sample mean(($\bar{X}$), caused by the continuous and constant variation of environmental factor. Therefore $\delta_{\varepsilon}$ can be easily calculated by the use of table of the standardized normal distribution.

• 한국환경농학회지
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• 제23권4호
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• pp.251-257
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• 2004

Analytical methods were developed to determine cyclosulfamuron residues in soil, water, rice grain and straw using high-performance liquid chromatography (HPLC) with ultraviolet absorption detection. In these methods, cyclosulfamuron was extracted with aqueous $Na_2HPO_4$/acetone and acetone/methanol mixture from soil and rice samples respectively. Liquid-liquid partition coupled with ion-associated technique, Florisil column chromatography, and solid-phase extraction (SPE) were used to separate cyclosulfamuron from interfering co-extractives prior to HPLC analysis. For water sample, the residue was enriched in $C_{18}$-SPE cartridge, cleaned up in situ, and directly subjected to HPLC. Reverse-phase HPLC under ion-suppression was successfully applied to determine cyclo-sulfamuron in sample extracts with the detection at its ${\lambda}_{max}$ (254 nm). Recoveries from fortified samples averaged $87.8{\pm}7.1%$ (n=12), $97.3{\pm}7.2%$ (n=12), $90.8{\pm}6.6%$ (n=6), and $78.5{\pm}6.7%$ (n=6) for soil, water, rice grain and straw, respectively. Detection limits of the methods were 0.004 mg/kg, 0.001 mg/L, 0.01 mg/kg and 0.02 mg/kg for soil, water, rice grain and straw samples, respectively.