• KSBB Journal
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• 제9권1호
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• pp.8-15
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• 1994

재조합 효모를 이용한 hirudin 발효생산조건의 최적화 연구를 수행하였다. Hirudin 유전자는 GAL10 promoter와 MFal 분비신호, GAL7 terminator와 결합되어 있다. 재조합 효모의 성장속도와 hirudin 최종 농도를 증가시키기 위하여 최적의 배지조성과 배양조건을 결정하였다. 최적의 배지조성과 배양조건은 yeast extract 40g/$\ell$, casamino acid 5g/$\ell$, 포도당 20g/$\ell$, galactose 30g/$\ell$, DO 50%, 온도 $30^{\circ}C$였다. 이 조건으로 2.5$\ell$ 발효조에서 회분식배양을 수행한 결과 비성장속도는 $0.13hr^{-1}$, 최종 건조균체농도는 30g cell/$\ell$, 최종 hirudin 농도는 64mg/$\ell$로 나타났다.

• 한국환경보건학회지
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• 제35권5호
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• pp.393-401
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• 2009

We isolated and identified a microorganism which was excellent for ammonia oxidation in the biological control of ammonia gas in odor producing materials from organic composting. The isolated strain was tested for growth characteristics and ammonia elimination efficiency under various conditions of temperature, pH, carbon concentration and ammonia concentration. The strain was isolated from a culture broth used in a $NO_2$ producing test with Griess-Ilosvay reagent. The results of 16S rRNA sequence from the isolated strain by using BLANST (Basic Local Alignment Search Tool) and confirming RDP (Ribosomal Database Project II) and ERRD (The European Ribosomal RNA Database) indicate that the strain is related to Delftia sp. UV-Spectrophotometer (Shimadzu, UVmini-1240) was used as a microbial growth test by measuring turbidity on OD660nm and ammonia concentration was measured by Spectrophotometer (HACH, DR-4000). The optimum growth culture conditions of the ammonia oxidizer Delftia sp. were $30^{\circ}C$, pH 7, glucose concentration 1.00% and $(NH_4)_2SO_4$ 0.5 g/l. Ammonia elimination efficiency was over 94% under the same conditions.

• Journal of Microbiology and Biotechnology
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• 제19권11호
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• pp.1431-1438
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• 2009

The role of plant growth-promoting rhizobacteria (PGPR) in the phytoremediation of heavy-metal-contaminated soils is important in overcoming its limitations for field application. A plant growth-promoting rhizobacterium, Serratia sp. SY5, was isolated from the rhizoplane of barnyard grass (Echinochloa crus-galli) grown in petroleum and heavy-metal-contaminated soil. This isolate has shown capacities for indole acetic acid production and siderophores synthesis. Compared with a non-inoculated control, the radicular root growth of Zea mays seedlings inoculated with SY5 can be increased by 27- or 15.4-fold in the presence of 15 mg-Cd/l or 15 mg-Cu/l, respectively. The results from hydroponic cultures showed that inoculation of Serratia sp. SY5 had a favorable influence on the initial shoot growth and biomass of Zea mays under noncontaminated conditions. However, under Cd-contaminated conditions, the inoculation of SY5 significantly increased the root biomass of Zea mays. These results indicate that Serratia sp. SY5 can serve as a promising microbial inoculant for increased plant growth in heavy-metal-contaminated soils to improve the phytoremediation efficiency.

• 한국환경보건학회지
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• 제25권3호
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• pp.7-12
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• 1999

Bioremediation is the technology to harness nature's biodegradative capabilities to remove or detoxify pollutions that threaten public health as environmental contaminants. Composting may become one of major bioremediation technologies for treating soils contaminated with petroleum if the fate of contaminants during composting is better understood Most composting research of petroleum was primarily focused on removing contaminant by optimizing composting conditions. Accordingly, laboratory feasibility studies may be useful to establish a realistic basis in co-composting complex substrate such as petroleum hydrocarbons. The purpose of this study was to assess the optimal conditions of kerosene biodegradation following supplementation with nutrient amendments under simulated composting conditions. Although it increased the growth of bacterial consortium, addition of co-substrates 0.5%(w/v) such as acetic acid, citric acid, glucose, and malic acid was not beneficial. Combination of nitrogen and phosphorous source enhanced kerosene biodegradation and reduced VOC evolution. These results showed that kerosene was able to utilize in bioremediation technology.

• 한국환경복원기술학회지
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• 제16권2호
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• pp.53-61
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• 2013

The present study elucidated the growth properties of Sasa borealis communities distributed in the lower layer of deciduous broadleaf forests in temperate zones and analyzed the correlation between the growth properties of S. borealis and positional environmental factors. The higher the culm height of S. borealis was, the higher the values of the leaf number, leaf area, and foliage layer thickness became. This might be because as the culm height of S. borealis increased, the acquisition of light sources became easier so that the biomass of leaves increased simultaneously for smooth anabolism. S. borealis seem to change their growth mode for smooth acquisition of light resources. The culm density of S. borealis and the leaf number, leaf area and foliage layer thickness of S. borealis did not show any clear correlation. The values of the culm height, leaf number, leaf area, and foliage layer thickness of S. borealis as the above altitude of the location of S. borealis increased. It seems like that growth conditions such as temperatures and winds are deteriorated as the above altitude of the location of S. borealis increased so that S. borealis becomes smaller. No clear correlations were shown between the physiochemical properties of soil and S. borealis' growth properties. It seems like that the growth of S. borealis complexly intertwined with diverse environmental factors and that due to the physiological integration of S. borealis, certain physiochemical properties do not unilaterally affect S. borealis' growth properties.

• 한국환경복원기술학회지
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• 제14권5호
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• pp.103-112
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• 2011

Old big tree transcends the simple meaning of trees as they are the natural monuments that embody the people's history and culture of this land. The Cultural Heritage Administration of Korea(CHA) defines and protects old big tree based not only on the size of the tree but also on its definitive cultural and natural factors such as value, implications, and originality. This research aims to identify and analyze the growth conditions, soil conditions and location character of 20 old big tree in Gyeongsangnamdo korea. The research examined the soundness of the arboreal form, the degree of damage on the bark, as well as the quantity of leafs levels to evaluate the overall condition of growth and development. Also, 9 elements such as soil texture, nitrogen and organic matter content, soil pH, phosphoric acid and EC were further analyzed The research analyzed in correlation of Growth condition and soil. Tree health related positivity that total nitrogen and organic matter. The result which analyzes location character, With natural monument old big trees raising a hand the area where is contiguous appeared with the fact that the farming village style where the rice field and the arable land of field etc. This research aimed at generating some foundational reference data for the analysis of the habitation and management conditions of natural monument old big tree within the Gyeongsangnamdo korea.

• 한국토양비료학회지
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• 제49권1호
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• pp.87-92
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• 2016

With the population growth and industrialization, the characteristics of discharged waste water and sewage have become more diverse. The removal of phosphorus (P) in the wastewater is essential for the prevention of eutrophication in the river and stream. This study was performed in order to estimate the field application of the Bacillus sp. 3434 BRRJ. Bacillus sp. 3434 BRRJ was cultured in the raw wastewater and synthetic medium at the 5 L reactor. The best optimum conditions for P removal by Bacillus sp. 3434BRRJ in the synthetic medium at the 5 L reactor were as follows: temperature, $30^{\circ}C$; P concentration, 20 mg/L; carbon sources, glucose + acetate (1:1); oxygen concentration, alternatively anaerobic and aerobic conditions. P removal efficiency under the optimum condition was 89.4%. In case of wastewater, P removal efficiency was 95.5% under controlled at $30^{\circ}C$. Through this study we confirmed that P removal by Bacillus sp. 3434BRRJ in case of wastewater was as effective as the synthetic medium. It is considered that Bacillus sp. 3434 BRRJ can be applied to the treatment of wastewater in order to biologically remove P from the wastewater on a large scale.

• Advances in concrete construction
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• 제16권2호
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• pp.97-106
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• 2023

This research endeavors to explore the enhancement of workforce economic efficiency through the application of nanotechnology, focusing on its economic implications. The findings of this investigation reveal that in recent years, surging global population growth and escalating demands for products and services have led to excessive resource consumption, resulting in adverse environmental consequences and altering environmental conditions-a phenomenon referred to as the economic growth dilemma. Entrepreneurs and economic stakeholders have begun to recognize the importance of sustainable development and the integration of environmental considerations into the production of goods and services. Within this context, knowledge-based economies have emerged as a driving force for sustainable business practices, particularly in the realm of nanotechnology. The integration of nanotechnology across various industries, including pharmaceuticals, agriculture, environmental management, and the chemical and petroleum sectors, as well as energy distribution, has yielded remarkable results. Consequently, this research aims to investigate the application and integration of nanotechnology in environmentally friendly silver nanoparticle production within select industries. Subsequently, it will examine the far-reaching implications of nanotechnology on economic growth and sustainable development.

• 한국토양비료학회지
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• 제50권5호
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• pp.452-461
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• 2017

This study was conducted to select an optimal approach to corn waste biochar (BC) application, and to evaluate the effects of combined application of BC and inorganic fertilizer (IF) on corn growth and soil chemical properties in a pot experiment. Corn growth differed with BC application timing and BC application levels. Based on the characteristics of corn growth in pot-based experiments, the selected optimal application conditions of BC were application of $500kg\;10a^{-1}$ at 20 days before sowing. Also, the chemical properties of the tested soil with BC after corn harvesting were significantly improved than those in the other treatments. In particular, soil pH and CEC regardless of application conditions were markedly increased by 0.04~0.19 units and $0.08{\sim}2.58coml_c\;kg^{-1}$ in BC treatments than without BC treatments. Additionally, combined application of BC and IF had greater effects on corn growth than single application of BC. Therefore, the results suggest using properly BC application conditions and a combination of BC and IF for effective corn cultivation in an upland field.

• 한국환경과학회지
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• 제13권10호
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• pp.883-890
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• 2004

The purpose of this study is to survey and analyze the growth, management and surrounding environment of the big and old trees in Kyoungju-si or the cultural assets alive in our history, and thereby, provide for some data useful to their reasonable protection and use of their surrounding areas. As a result of surveying the growth conditions of the big and old trees, it was found that the height of new grass was 10.5cm on average, the activity scale of the wood was 7.2k$\Omega$, the soil hardness was $16.7kg/cm^2$, the soil acidity was pH 4.8, and the soil moisture was $13.3\%$. Such findings suggest that the soil has been acidified by people's frequent passages, but that the other growth conditions are more or less normal. Hence, it is desirable to secure a sufficient space around the trees or reduce people's stamping pressure with some mechanisms. On the other hand, the visible conditions of the trees were found more or less normal, but many trees remained cut or barked (with some cavities), requiring an optimal treatment or measure. Lastly, as the population has decreased in the suburban traditional villages, the surrounding environment seems to be less vulnerable to people's frequent visits. Nevertheless, in consideration of the fact that there are only a few public space for the villagers, it is deemed necessary to rearrange or maintain some parts of the surrounding environment as public space for villagers or hikers.