• Title/Summary/Keyword: Environmental Restriction

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Optimization of wastewater electrolysis using life cycle assessment and simulated annealing

  • Chun Hae Pyo;Chon Hyo-Taek;Kim Young Seok
    • 한국지구물리탐사학회:학술대회논문집
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    • 한국지구물리탐사학회 2003년도 Proceedings of the international symposium on the fusion technology
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    • pp.518-521
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    • 2003
  • LCA (Life Cycle Assessment), that unifies the scale of various environmental impacts, and simulated annealing are applied to optimizing electrolysis of wastewater from PCB (Printed Circuit Board) production. The changes of environmental impact can be quantified with LCA and the total changes of environmental impacts can be expressed as a function of power consumed, Cu recycled, $Cl_2$, NOx and SOx discharged through restriction of feasible reactions. In a single-variate condition, the environmental optimum can be easily obtained through plotting and comparing each environmental impact value. In 8V potentiostatic electrolysis, the lowest environmental impact can be achieved after 90min. To optimize a multi-variate conditional system, simulated annealing can be applied and this can give the quick and near optimum in complex systems, where many input and output materials are involved, through experimentally measured values without a theoretical modeling.

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Modified T-RFLP Methods for Taxonomic Interpretation of T-RF

  • Lee, Hyun-Kyung;Kim, Hye-Ryoung;Mengoni, Alessio;Lee, Dong-Hun
    • Journal of Microbiology and Biotechnology
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    • 제18권4호
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    • pp.624-630
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    • 2008
  • Terminal restriction fragment length polymorphism (T-RFLP) is a method that has been frequently used to survey the microbial diversity of environmental samples and to monitor changes in microbial communities. T-RFLP is a highly sensitive and reproducible procedure that combines a PCR with a labeled primer, restriction digestion of the amplified DNA, and separation of the terminal restriction fragment (T-RF). The reliable identification of T-RF requires the information of nucleotide sequences as well as the size of T-RF. However, it is difficult to obtain the information of nucleotide sequences because the T-RFs are fragmented and lack a priming site of 3'-end for efficient cloning and sequence analysis. Here, we improved on the T-RFLP method in order to analyze the nucleotide sequences of the distinct T-RFs. The first method is to selectively amplify the portion of T-RF ligated with specific oligonucleotide adapters. In the second method, the termini of T-RFs were tailed with deoxynucleotides using terminal deoxynucleotidyl transferase (TdT) and amplified by a second round of PCR. The major T-RFs generated from reference strains and from T-RFLP profiles of activated sludge samples were efficiently isolated and identified by using two modified T-RFLP methods. These methods are less time consuming and labor-intensive when compared with other methods. The T-RFLP method using TdT has the advantages of being a simple process and having no limit of restriction enzymes. Our results suggest that these methods could be useful tools for the taxonomic interpretation of T-RFs.

Biological Nitrogen Removal System의 세균 군집 분석 (Structure of Bacterial Communities in Biological Nitrogen Removal System)

  • 김경미;이상일;이동훈
    • 미생물학회지
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    • 제42권1호
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    • pp.26-33
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    • 2006
  • 생물학적 질소 제거(Biological nitrogen removal; BNR) 시스템의 효율적인 처리 공정을 이재하기 위하여 질산화 반응조 내 세균 군집 구조를 16S rRNA 유전자의 PCR 및 terminal restriction fragment length polymorphism (T-RELP)방법을 이용하여 분석하였다. 본 연구에서 사용한 BNR 시스템은 국내에서 비교적 많이 적용되고 있는 부상여재를 이용한 고도처리 시스템, Nutrient Removal Laboratory 시스템, 반추기법을 이용한 영양염류 처리 Sequencing Batch Reactor (SBR)시스템이었고, 실험 결과 모든 시료에서 암모니아 산화 세균과 $\beta-proteobacteria$에 해당되는 말단 단편을 확인할 수 있었다. 암모니아 산화세균 군집에서 유래된 말단 단편의 염기서열을 분석한 결과 SBR공정에서는 Nitrosomonas와 Nitrosolobus에 속하는 군집 이 우점종임을 확인할 수 있었다. 그러나 다른 두 공정들에서는 $\beta-proteobacteria$에 속하는 미배양 균주와 Cardococcus australiensis와 염기서열 유사도가 높은 군집이 우점하였다. 또한, 암모니아산화 세균군집을 분석한 결과, SBR 공정이 암모니아 산화세균의 농화 배양에 가장 효과적인 것으로 나타났다. 이러한 결과는 각 BNR 시스템에 동일한 폐수가 유입되었음에도 불구하고 서로 다른 세균 군집 구조를 형성하고 있음을 의미한다.

Performance Evaluation of Location Estimation System Using a Non Fixed Single Receiver

  • Myagmar, Enkhzaya;Kwon, Soon-Ryang
    • International Journal of Contents
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    • 제10권4호
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    • pp.69-74
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    • 2014
  • General location aware systems are only applied to indoor and outdoor environments using more than three transmitters to estimate a fixed object location. Those kinds of systems have environmental restrictions that require an already established infrastructure. To solve this problem, an Object Location Estimation (OLE) algorithm based on PTP (Point To Point) communication has been proposed. However, the problem with this method is that deduction of performance parameters is not enough and location estimation is very difficult because of unknown restriction conditions. From experimental tests in this research, we determined that the performance parameters for restriction conditions are a maximum transmission distance of CSS communication and an optimum moving distance interval between personal locations. In this paper, a system applied OLE algorithm based on PTP communication is implemented using a CSS (Chirp Spread Spectrum) communication module. A maximum transmission distance for CSS communication and an optimum moving distance interval between personal locations are then deducted and studied to estimate a fixed object location for generalization.

Muscle-Specific Creatine Kinase Gene Polymorphisms in Korean Elite Athletes

  • Kang, Byung-Yong;Kang, Chin-Yang;Lee, Kang-Oh
    • Toxicological Research
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    • 제19권2호
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    • pp.115-121
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    • 2003
  • In view of the importance of muscle-specific creatine kinase (CKMM) gene as a genetic factor for athletic performance, we investigate the relationship between elite athletic performance and two restriction fragment length polymorphisms (Ncol and Taql RFLPs) in the CKMM gene. Genomic DNA was extracted from white blood cells of 98 unrelated male Korean elite athletes and 04 sedentary controls, respectively. Two genetic polymorphisms in the CKMM gene were detected by the polymerase chain reaction and the digestion with restriction endonucleases, Ncol and Taql, respectively. There were no significant associations between two genetic polymorphisms in the CKMM gene and elite athletic performance or clinical parameters in our subjects. Therefore, these findings suggest that two genetic polymorphisms in the CKMM gene may not be useful as genetic markers to predict the athletic performance in male Koreans.

Nitrospira Community Composition in Nitrifying Reactors Operated with Two Different Dissolved Oxygen Levels

  • Park, Hee-Deung;Noguera, Daniel R.
    • Journal of Microbiology and Biotechnology
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    • 제18권8호
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    • pp.1470-1474
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    • 2008
  • Nitrospira is a dominant member of nitrite-oxidizing bacteria (NOB) in nitrifying bioreactors as well as in natural habitats. In this study, Nitrospira NOB were investigated in the two nitrifying reactors operated with high and low dissolved oxygen (DO) concentrations for a period of 300 days. Phylogenetic and terminal restriction fragment length polymorphism analyses based on 168 rRNA gene sequences revealed that the Nitrospira community compositions of the two reactors during the early period related to group 1 and half of the Nitrospira community composition shifted to group 2 in the high-DO reactor after day 179, although there was no significant change in the low-DO reactor. These results suggested that DO was an important factor affecting Nitrospira community compositions in the nitrifying reactors.

Intermittent Fasting: a Promising Approach for Preventing Vascular Dementia

  • Yoon, Gwangho;Song, Juhyun
    • 지질동맥경화학회지
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    • 제8권1호
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    • pp.1-7
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    • 2019
  • Vascular dementia is the most common neuropsychiatric syndrome and is characterized by synaptic dysfunction, neuroinflammation, and cognitive dysfunction. Vascular dementia is associated with various environmental, genetic, and lifestyle risk factors. Recent research has focused on the association between vascular dementia and dietary patterns, suggesting that dietary regulation leads to better control of energy metabolism, improvements in brain insulin resistance, and the suppression of neuroinflammation. Intermittent fasting is a calorie-restriction method known to be more effective in promoting fat loss and regulating the impairment of glucose metabolism as compared with other dietary restriction regimens. Herein, the authors review the effects of intermittent fasting with regard to vascular dementia based on recent evidence and propose that intermittent fasting could be a therapeutic approach for ameliorating vascular dementia pathology and preventing its onset.

Diversity and Polymorphism in AHL-Lactonase Gene (aiiA) of Bacillus

  • Huma, Nusrat;Shankar, Pratap;Kushwah, Jyoti;Bhushan, Ashish;Joshi, Jayadev;Mukherjee, Tanmoy;Raju, Sajan C.;Purohit, Hemant J.;Kalia, Vipin Chandra
    • Journal of Microbiology and Biotechnology
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    • 제21권10호
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    • pp.1001-1011
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    • 2011
  • To explore bacterial diversity for elucidating genetic variability in acylhomoserine lactone (AHL) lactonase structure, we screened 800 bacterial strains. It revealed the presence of a quorum quenching (QQ) AHL-lactonase gene (aiiA) in 42 strains. These 42 strains were identified using rrs (16S rDNA) sequencing as Bacillus strains, predominantly B. cereus. An in silico restriction endonuclease (RE) digestion of 22 AHL lactonase gene (aiiA) sequences (from NCBI database) belonging to 9 different genera, along with 42 aiiA gene sequences from different Bacillus spp. (isolated here) with 14 type II REs, revealed distinct patterns of fragments (nucleotide length and order) with four REs; AluI, DpnII, RsaI, and Tru9I. Our study reflects on the biodiversity of aiiA among Bacillus species. Bacillus sp. strain MBG11 with polymorphism (115Alanine > Valine) may confer increased stability to AHL lactonase, and can be a potential candidate for heterologous expression and mass production. Microbes with ability to produce AHL-lactonases degrade quorum sensing signals such as AHL by opening of the lactone ring. The naturally occurring diversity of QQ molecules provides opportunities to use them for preventing bacterial infections, spoilage of food, and bioremediation.

환경물질을 제외한 전자부품의 신뢰성평가 방법 연구 - Pb와 Br을 제거한 PWB를 중심으로 - (Reliability Assessment Methods for Electronic Component Removed Environmental Materials - focused on Printed Wiring Board without Pb and Br -)

  • 이종범;조재립
    • 한국품질경영학회:학술대회논문집
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    • 한국품질경영학회 2006년도 춘계학술대회
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    • pp.381-391
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    • 2006
  • When the environmental materials of the raw and sub material to applied in electronic goods removed, the reliability assessment and the forecasting is stand on the low level and in case of neglect, it is acting as latent defect that can be connected to large size disaster. In this research, the study on the management method of environmental restriction substances which is recognized as important element in the reliability assessment about environmental material of electronic goods and the study on reliability assessment method of PWB without environmental material are progressed

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트립톨라이드가 식이제한에 의한 수명연장과 노화관련 질환에 미치는 영향 (Triptolide Mimics the Effect of Dietary Restriction on Lifespan and Retards Age-related Diseases in Caenorhabditis elegans)

  • 백선미;박상규
    • 생명과학회지
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    • 제28권8호
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    • pp.931-937
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    • 2018
  • 뇌공등에 함유되어있는 트립톨라이드는 뛰어난 항염증, 항산화 효능을 가지고 있음이 보고되었다. 예쁜꼬마선충을 이용한 이전 연구에서 트립톨라이드의 섭취가 개체의 항스트레스 효능을 높이고, 수명을 연장시킴이 밝혀졌다. 본 연구에서는 트립톨라이드에 의한 수명연장에 관여하는 세포 내 기전과 트립톨라이드가 노화관련 질환인 당뇨병과 알츠하이머병에 미치는 영향을 평가하였다. 트립톨라이드는 인슐린/IGF-1-like 신호전달 저하에 의한 수명연장 돌연변이인 age-1과 미토콘드리아 전자 전달계 저하에 의한 수명연장 돌연변이인 clk-1의 수명을 유의적으로 증가시킨 반면, 식이제한 유도 돌연변이인 eat-2의 수명에는 유의적인 변화를 유도하지 못했다. 또한 박테리아 희석을 이용한 식이제한에 의해 연장된 수명을 추가적으로 더 연장시키지 못했다. 트립톨라이드 섭취는 고농도의 당 섭취에 의한 체내 독성과 사람 아밀로이드 베타 형질전환 유전자로 인한 체내 독성을 유의적으로 저하시켰다. 이러한 결과들은 트립톨라이드에 의한 수명연장이 식이제한에 의한 수명연장 기전과 중복되며, 트립톨라이드가 노화관련 질환을 저해하는 효능이 있음을 보여준다. 따라서, 트립톨라이드는 식이제한 효능을 대체할 수 있는 식의약품 개발에 활용될 수 있다.