• Journal of Nutrition and Health
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• 제16권4호
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• pp.243-252
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• 1983

This study was undertaken to invesigate the effect of early nutritional deprivation and environment on neurotransmitter concentrations and behavior in later life. The restoring process of rats fed foods ad libitum after 50% restriction of the casein or the Korean diet during the prenatal and/or the lactating periods was observed. There were two rearing conditions, isolated and enriched, after weaning. Behavioral development was measured by the Y- shaped water maze and the open field test. The neurotransmitters were analyzed after sacrifice at the age of 21 weeks. The results are summarized as follows. 1) The body weight impairment by dietary restriction during the prenatal and lactating periods could be restored within 18 weeks after weaning in case of living in a classical cage. The effect of quantitative restriction was bigger in the Korean diet than in the casein diet. 2) The brain weight was decreased by nutritional deprivation. Environmental enrichment increased it slightly. 3) The concentration of neurotransmitters, norepinephrine, dopamine, and serotonin, were not shown any traces of the dietary restriction at the age of 21 weeks. 4) In the maze test, the deprived rats made more errors than the nourished and the rats fed the Korean diet more than those fed the cascin dict. The environmental enrichment could decrease the number of errors. 5) In the open field test, the dietary deprived groups showed less reaction time, more squares entered in the field, and less number of fecal boli than the nourished among the environmentally isolated rats. However, rats living in the enriched cage without experience of nutritional stress showed the lowest emotionality and the elevated exploratory activity.

• 한국물환경학회지
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• 제30권4호
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• pp.418-428
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• 2014

The purpose of this study is to predict the spatio-temporal changes in land uses and to evaluate land-based pollutant loads in the future under Total Water Pollution Load Management System using CLUE-S model. For these ends, sensitive parameters of conversion elasticities in CLUE-S model were calibrated and these calibrated parameters of conversion elasticities, level II land cover map of year 2009, and 7 driving factors of land use changes were used in predicting future land uses in 2002 with two scenarios(Scenario 1: non area restriction, Scenario 2: area restriction). This projected land use map of 2020 was used to estimate land-based pollutant loads. It was expected that urban areas will increase in 2020 from both scenarios 1 and 2. In Scenario 1, urban areas are expected to increase within greenbelt areas and deforest would be expected. Under Scenario 2, these phenomena were not expected. Also the results of estimation of BOD and TP pollutant loads, the BOD difference between scenarios 1 and 2 was 719 kg/day in urban areas and TP difference was 17.60 kg/day in urban areas. As shown in this study, it was found that the CLUE-S model can be useful in future pollutant load estimations because of its capability of projecting future land uses considering various socio-economic driving factors and area-restriction factors, compared with conventionally used land use prediction model.

• Asian-Australasian Journal of Animal Sciences
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• 제18권10호
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• pp.1368-1374
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• 2005

To investigate the genetic relationships among various cattle breeds, bovine mtDNA D-loop region was used in 411 animals of 18 cattle breeds, including 8 Asian Bos taurus, 7 European Bos taurus, 1 Asian Bos indicus, and 2 African Bos indicus. The size of amplified PCR products from mtDNA D-loop region was 964 bp and the products were digested by 15 different restriction enzymes. Two different band patterns were identified in eight restriction enzymes (BstXI, Hae III, Msp I, Apa I, Taq I, Alu I, BamH I, EcoN I) and the rest of restriction enzymes showed more than 3 different band patterns among which Apo I and MspR9 resulted in 7 different restriction patterns. The genotypes, number of haplotype, effective number of haplotype, and degree of heterozygosity were analyzed. Based on all the PCR-RFLP data, different haplotypes were constructed and analyzed for calculating genetic distances between these breeds using Nei's unbiased method and constructing a phylogenetic tree.

• 한국환경성돌연변이발암원학회지
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• 제22권3호
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• pp.199-204
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• 2002

It has been known that Ganoderma lucidum and Lentinus edodes have anticancer activity and immune enhancing activity. These two mushrooms were grown in liquid culture and harvested. From these mycelia, DNA was isolated and EtBr-CsCl density gradient ultracentrifugation was performed to purify it further. Then mitochondrial DNA was isolated by bisbenzimide-CsCl density ultracentrifugaton. Mitochondrial DNA of Ganoderma lucidum was digested by restriction enzymes, EcoR I, Hind Ⅲ, and Pst I, then electrophoresed. It showed 12, 22, 4 fragments. Mitochondrial DNA of Lentinus edodes was digested by EcoR I. Electric pattern showed 6 fragments. 4 fragments had appeared by Pst 1 digested mitochondrial DNA. Hind ill couldn't digest mitochondrial DNA of Lentinus edodes. Mitochondrial DNA of fusants was isolated to compare to those of parents. The results showed that fusant P₂S₄has new, recombined mitochondrial DNA. But P₂S₄had the same DNA that Ganoderma lucidum had.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제1권2호
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• pp.93-97
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• 1997

The naturally occurring plasmids of Vibrio species have been isolated in part to investigate their genetic traits. Among six different Vibrio species tested, Vibrio anguillarum, Vibrio fluvialis, Vibrio vulnficus, Vibrio mimicus and Vibrio furnissi did not show any presence of plasmid. One environmental isolate of Vibrio parahaemolyticus harboring plasmid was observed. The isolated plasmid was 8.7 kb by analysis with restriction endonuclease digestion. No common feature was shown relationships between the presence of plasmid and resistance against commonly used antibiotic compounds from the tested Vibrios.

• 환경영향평가
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• 제24권6호
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• pp.549-560
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• 2015

농공단지에 입주제한된 31개 업종의 업종별 배출특성에 따른 화학적 유해성을 근거로 업종별 환경영향을 계량화하여 입지규제 우선순위을 도출하였다. 농공단지에 입주제한된 31개 업종의 환경상의 악영향과 환경기술발전에 따른 환경유해성 저감을 동시에 고려한 합리적 토지이용 관리방안을 수립하기 위하여 업종별로 수계, 토양 및 대기의 매체별 배출량 및 배출물질 $LC_{50}$ 자료에 의해 산정된 인체위해성과 생태위해성을 노출평가와 독성평가로 분석하는 화학적 등급화기법(CRS : Chemical Ranking and Scoring System)에 PRTR (Pollutant Release and Transfer Registers) 자료를 활용하였다. 농공단지 폐수배출관련 31개 업종에 대한 환경유해성을 도출한 결과에 의하면 2012년 기준으로 도금 착색 및 기타 표면처리강재 제조업, 생물학적 제제 제조업, 동 제련정련 및 합금 제조업, 솜 및 실 염색가공업, 그외 기타 1차철강 제조업, 기타 비철금속 압연압출 및 연신제품 제조업 등 6개 업종은 환경영향 점수가 상대적으로 낮게 산출되어 제조공정상에서 특정수질유해물질을 배출허용기준 이하로 배출된다고 인정하는 경우에 농공단지 입주제한을 완화시킬 수 있는 업종으로 평가된다.

• 한국지역지리학회지
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• 제15권2호
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• pp.282-291
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• 2009

본 연구는 1915년과 2005년의 공간정보를 이용하여 규제와 관련된 공간 갈등을 예측하고자 한 연구다. 이를 위해 연구지역인 평창의 상대적인 공간 밀도의 차이와 그에 따른 환경규제 갈등을 예측하고자 하였다. 먼저 연구지역의 건물 밀도를 이용하여 공간구조를 분석한 결과 1915년과 비교하여 2005년의 건물 집적 정도가 강화되었음을 확인할 수 있었다. 변화 유형은 신규로 거점을 형성하고 성장한 유형, 인접한 중심 거점으로의 집중이 진행된 유형, 세력이 전반적으로 약화된 유형, 큰 변화를 보이지 않는 유형 등으로 분류할 수 있었다. 다음으로 연구지역에서 예상되는 환경규제의 장 단기적 갈등을 분석한 결과 진부면 일대와 평창읍의 도시 외곽지역, 그리고 31번 국도변 등은 장 단기적으로 개발과 보전의 갈등이 클 것으로 예상되었다. 그리고 대관령면은 단기적으로는 갈등이 크지만 장기적으로는 갈등이 약화될 것으로 예상되었으며, 방림면은 현 시점에는 갈등이 약하지만 장기적으로는 갈등이 발생할 가능성이 높은 것으로 예상되었다. 이러한 연구 결과는 지역의 공간 갈등을 최소화기 위한 기초자료로 활용될 수 있을 것으로 기대된다.

• 한국환경과학회지
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• 제1권2호
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• pp.93.2-97
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• 1992

The naturally occurring plasmids of Vibrio species have been isolated in part to investigate their genetic traits. Among six different Vibrio species tested, Vibrio anguillarum, Vibrio fluvialis, Vibrio vulnificus, Vibrio mimicus and Vibrio furnissi did not show any presence of plasmid. One environmental isolate of Vibrio pnrahemolyticus harboring plasmid was observed. The isolated plasmid was 8.7 kb by analysis with restriction endonuclease digestion. No common feature was shown relationships between the presence of plasmid and resistance against commonly used antibiotic compounds from the tested Vibrios. Key words . plasmid, Vibrio anguillarum, Vibrio fluvialis, Vibrio uulnincus, Vibrio mimicus, Vibrio furnissi, Vibrio parahemolyticus.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2007년도 춘계학술대회
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• pp.823-828
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• 2007

In compare with a major developed countries, Korea consumes much energy, but also eliminates much carbon dioxide. Agricultural sector eliminate much carbon dioxide than industrial sector. In Kyoto protocol, Korea needs to reduce carbon dioxide. One way to reduce carbon dioxide is utilization of biomass in rural area. This paper focus on utilization of biomass in rural area. If use 20% potential amount of biomass, it obtain 50% of TPES on agricultural sector. The condition of utilization biomass is connected with agricultural policy, environmental policy, and energy policy. And environmental restriction keep pace with economical incentive.

• 환경생물
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• 제36권3호
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• pp.352-358
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• 2018

본 연구는 PCR 기반 RFLP (Restriction Fragment Length Polymorphism; 제한절편 길이 다형성) 분자기법을 활용하여 난 및 치어 대상 납자루아과 어류 3종의 동정을 좀 더 빠르고 정확하게 파악하고 납자루아과 어류의 종별 산란양상 및 번식생태 이해에 대한 기여가 목적이다. 본 연구를 위해 기존 선행된 문헌자료를 확인하고 납자루아과 어류가 2종 이상 동서하고 있는 지역을 확인하여 현지조사를 수행하였다. 현지조사 결과 확인된 납자루아과 어류는 묵납자루(Acheilognathus signifer), 줄납자루(A. yamatsutae) 및 각시붕어(Rhodeus uyekii)로 총 3종이 확인되었으며, 확인된 납자루아과 어류와 동서하고 있는 숙주조개(작은말조개; Unio douglasiae sinuolatus)를 채집하여 숙주조개 속 납자루아과 어류의 난 및 치어를 확보하였다. 현지조사 결과 확인된 납자루아과 어류 3종을 대상으로 미토콘드리아 DNA COI과 cyt b 유전자 염기서열을 비교하여 각각 종별로 특이성을 지닌 부위(단일염기변이; Single Nucleotide Variation: SNV)에 맞는 제한효소를 선정하였고, 숙주조개 속 난 및 치어를 대상으로 genomic DNA를 추출하여 PCR-RFLP 실험을 수행한 결과 현지조사 시 확인된 납자루아과 어류 3종의 독특한 제한절편 길이 양상을 전기영동을 통하여 확인하였다. 본 연구를 통해 묵납자루, 줄납자루 및 각시붕어의 종을 판별할 수 있는 RFLP 마커를 개발하였으며, 숙주조개 난 및 치어를 대상으로 정확한 종의 동정을 보다 빠르고 효과적으로 수행하여 각각 납자루아과 종별 산란양상을 보다 정확히 규명하고 향후 이들 자연개체군의 효과적인 유지, 관리 및 보전 방법 개발에 유용하게 활용될 수 있을 것으로 판단된다.