• Microbiology and Biotechnology Letters
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• v.32 no.2
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• pp.179-183
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• 2004

Enterovirus isolation was attempted from samples obtained from aseptic meningitis-suspected patients in hospitals in Busan during 2002. Enteroviruses were found in 83 of 703 cases. Echovirus serotypes 2, 3, 6, 7, 9, 13, 25, 29, 30 were isolated in 72 cases while coxsackievirus serotypes B3 and B4 were isolated in 11 cases. The occurrence was found to be distributed from April thru November with the highest rate during June and July. The strains of Vero and HEp-2 of echovirus and coxsackievirus, respectively, are highly infectious. Age distribution of patients, 61 patients in Enteroviruses and 11 patients in coxsackievirus, respectively, occurred in 0 to 10 year-olds. The sex distribution of the patients is as follows, 52 males (62.7％), 31 females (37.3％). Occurrence rate was found to be higher in male patients. Electron micrograph of echovirus and coxsackievirus show that they are small nonenevolped, isometric-shaped viruses. Isolated RNA from strains of echovirus and coxsackievirus showing cytopathic effects were used to undergo nested PCR which resulted in a 437 bp single band in all the strains.

• Journal of Microbiology and Biotechnology
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• v.9 no.6
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• pp.832-838
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• 1999

Two different types of lipases (lipase I and lipase II) secreted into culture medium by Rhizopus sp. L-I were purified using a hydrophobic chromatography and were partially characterized. Both enzymes were monomeric as revealed by SDS-PAGE and gel filtration. The molecular masses of the enzymes were identified as 45 kDa (lipase I) and 69 kDa (lipase II). The isoelectric points were estimated to be 3.6 and 5.2 for lipase I and lipase II, respectively. pH and temperature activity optima for lipase I were as 7.5 and $50^{\circ}C$, respectively, whereas the corresponding parameters for lipase II were 6.0 and $45^{\circ}C$. The amino terminal sequences of lipase I and lipase II, determined by Edman degradation, were found to be Leu-Val-Met-Ile-Gln-Arg and Leu-Val-Met-Lys-Gln-Arg, respectively. By western blotting analysis, the two lipases were found to have a common antigenic determinant. Immuno-electron cytochemistry conducted with polyclonal anti-lipase I antibody indicated the enzyme located in both the periplasm and the adjacent vesicles of fungal hyphae. Fortunately, the sites on the cell envelope where lipase was exported into the culture medium was also identified.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.13 no.6
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• pp.490-500
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• 1988

In recent years the acquisition, processing and interpretation of three dimensional seisimic data, for the purpose of locating gas and reservoirs, have become practical. This paper exlores one way in which the volume data can be searched and visualized, which may aid the interpreter. The illusion of looking at a three dimensional volume can be obrained by fusing a stereoscopic pair of pictures. Each picture can be made by projecting each data point of the volume into a plane from a point where the eye is placed. The data valuse along any projection line can be summed to form the picture, or only a segment along the line can be selected. By selective projection, the volume can be searched and obscuring layers removed. The stereoscopic pictures show the physical models in there ture spatial positions. Projection of the envelope function of the seismic traces is shown to give improved depth perception compared with projection of the position amplitudes.

• KIEAE Journal
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• v.17 no.3
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• pp.5-13
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• 2017

Purpose: Various studies have been performed to address the issue of increased energy use by buildings. In particular, research on complex envelopes that combines diverse envelope element techniques is currently in progress in the building sector. The present study aimed to develop an awning system using a light shelf, and to verify its validity through performance evaluation. Method: In the present study, a testbed was established for the performance evaluation of the awning system using a light shelf, and the uniformity ratio and lighting energy consumption were compared and analyzed relative to those with no awning and light shelf installation (Case 1), awning installation (Case 2), and light shelf installation (Case 3). Result: 1) In the present study, an awning system using a light shelf (Case 4) where an opening is made on the awning screen and natural light can be introduced through the light shelf located at the bottom was developed. 2) The optimum standard for Case 4 obtained through the performance evaluation was a 0.6m lighting length and a 2m extension length at a light shelf angle of $30^{\circ}$. 3) Case 4 with the optimum standard had a 5.5% lower uniformity ratio than Case 2, but had a higher uniformity ratio than Case 1 and Case 3. 4) Case 4 with the optimum standard showed 13.3%, 44.6%, and 0%~8.7% lighting energy reductions compared to Case 1, Case 2, and Case 3, respectively. 5) Based on the above results, Case 4 suggested in the present study was found to be effective for indoor light environment improvement and lighting energy reduction.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.8C
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• pp.822-829
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• 2009

This paper proposes a new method of improving the quality of High Efficiency Advanced Audio Coding (HE-AAC). HE-AAC encodes input source by allocating bits for each scalefactor bands appropriately according to human ear's psychoacoustic property. As a result, insufficient bits are assigned to the bands which have relatively low energy. This imbalance between different energy bands can cause decreasing of sound quality like musical noise. In the proposed system, a Linear Prediction (LP) module is combined with HE-AAC as a pre-processor to improve sound quality by even bits distribution. To apply accurate human being's psychoacoustic property, the psychoacoustic model uses Fast Fourier Transform (FFT) spectrum of original input signal to make masking threshold. In its implementation, masking threshold of psychoacoustic model is normalized using the LP spectral envelope in prior to quantization of the LP residual. Experimental result shows that, the proposed algorithm allocates bits appropriately for insufficient bits condition and improves the performance of HE-AAC.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.9
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• pp.420-426
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• 2018

Acoustic Quanta, which British Physicist Dennis Gabor created, is the theoretical background for granular synthesis and has influenced many computer music artists and sound designers. Acoustic Quanta is a very short sound burst, lasting only 1 to 100 ms. Granular synthesis is a sound synthesis technique which slices original sound into sound grains and re-combines them into a new acoustic event. Concept of sound grain is borrowed from the acoustic quanta. Granular Synthesis can make very unique sound, so that it can be useful in many ways, especially in sound design. This paper presents concept of acoustic quanta and granular synthesis. It then discusses making a synthesizer as an implementation of synchronous granular synthesis and its applications on sound design. As a result, the duration of acoustic quanta should range between 0.239 and 33.367 ms, in consideration of audible frequencies, which is different from the original concept of the acoustic quanta.

• Korean Journal of Animal Reproduction
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• v.26 no.4
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• pp.353-360
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• 2002

Although successful pronuclear formation and apposition were seen in porcine oocytes following mouse sperm injection, little is known on the morphology of male and female pronuclei following sperm injection. The objective of this study is to describe the ultrastructure of porcine zygote following murine sperm injection in relation to the chronology of pronuclear S phase. At 40h ~ 44h following in vitro maturation, Cumulus cells were removed in TCM-HEPES with 0.1% hyaluronidase. Then, spermatozoa was injected into the cytoplasm of oocytes. After. injection, all oocytes were transferred to NCSU23 medium and cultured at 39$^{\circ}C$ under 5% $CO_2$ in air. Oocytes were fixed in 2% glutaraldehyde in Dulbeccos phosphate-buffered saline and observed by Transmission Electron Microscopy. Nuclear precursor bodies were observed in each pronucleus. A cluster of large and small granules was attached in the nucleolus precursor body. After the apposition of male and female chromatin, chromatin condensation was observed throughout the nucleoplasm and nucleolus precursor bodies and condensed chromatin in contact with clusters of small and large granules and the nuclear envelope were found in apposed pronuclear regions. These results suggest that non-species specific nuclear cytoplasmic interactions take place during pronuclear formation and apposition following sperm injection.

• Journal of Biomedical Engineering Research
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• v.34 no.2
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• pp.63-68
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• 2013

Activeness of exercise is critical for stroke rehabilitation so that application of stimulation in response to patient's intention would be effective in FES cycling. The purpose of this study was to investigate the relationship between muscle reaction force (MRF) and electromyogram (EMG) during cycling exercise, for the future usage of MRF as patients' intention signal. Seven young men ($24{\pm}1.63$ yrs) participated in this study. Cycling speed was set to 20 RPM and 60 RPM. MRF and EMG were measured in the vastus lateralis muscle of right leg. Active cycling was performed at the maximal load (16 Nm) of an ergometer. Angle dependent artifact in MRF was measured from passive cycling and was subtracted from the MRF of active cycling. The delay of MRF with respect to EMG envelope and their correlation coefficients were derived from the best of cross correlation. MRF was significantly correlated with EMG amplitude in all subjects (p<0.01). Their mean correlations were 0.84 and 0.91 for 20 RPM and 60 RPM, respectively. Mean delay in MRF was 59.14 ms and 53.14 ms for 20 RPM and 60 RPM, respectively. The result suggests that MRF can be used to assess patient's intention for exercise as a substitute to EMG. The method may be applied to FES cycling to encourage patient's effort which is critical for stroke rehabilitation.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.24 no.7
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• pp.555-561
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• 2014

Acoustic Emission technique is widely applied to develop the early fault detection system, and the problem about a signal processing method for AE signal is mainly focused on. In the signal processing method, envelope analysis is a useful method to evaluate the bearing problems and wavelet transform is a powerful method to detect faults occurred on rotating machinery. However, exact method for AE signal is not developed yet for the rotating machinery diagnosis. Therefore, in this paper two methods which are processed by Hilbert transform and DET for feature extraction. In addition, we evaluate the classification performance with varying the parameter from 2 to 15 for feature selection DET, 0.01 to 1.0 for the RBF kernel function of SVR, and the proposed algorithm achieved 94 % classification of averaged accuracy with the parameter of the RBF 0.08, 12 feature selection.

• The Journal of Korean Society of Virology
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• v.29 no.4
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• pp.221-233
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• 1999

Prospect Hill Virus (PHV) is the well known serotype of hantavirus, a newly established genus in family Bunyaviridae. Extensive studies have upheld the original view of PHV genetics with three genes such as nucleocapsid (N) protein, envelope proteins (G1, G2) and RNA dependent RNA polymerase. In this study, we report the existence of additional gene that is encoded in an overlapping reading frame of the N protein gene within S genome segment of PHV. This gene is expected to encode a nonstructural small (NSs) protein and it seems to be only found in PHV infected cell. The presence and synthesis of NSs protein could be demonstrated in the cell infected with PHV using anti-peptide sera specific to the predicted amino acid sequence deduced from the second open reading frame. Ribosomal synthesis of this protein appears to occur at AUG codon at the 83rd base of S genome segment, downstream of N protein initiation codon. This protein is small in size (10.4 KDa) and highly basic in nature. The expression strategy of NSs protein appears that a signal mRNA is used to translate both N and NSs protein in PHV infected cell. 10 KDa protein in virus infected cell lysates can bind to mimic dsRNA. This fact strongly suggests that NSs protein may be involved in virus replication on late phase of viral life cycle.