• The Plant Pathology Journal
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• v.17 no.2
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• pp.94-100
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• 2001

Roots of Chinese cabbage (Brassica campestris var. chinensis) seedlings infected with Plasmodiophora brassicae were examined by light and electron microscopy to reveal histopathological changes related to pathogenesis in the susceptible host. The pathogen colonized the cortex and partly the stele as well, invading up to the xylem. Gall tissues could be differentiated from the initially infected tissues, involving less compact organization and new vascular development. The infected cells were much hypertrophied, and contained one to several plasmodia. Except cellular hypertrophy, no pathological ultrastructural modification was noted in the infected calls. Infected cytoplasm became dense with ground cytoplasm, inconspicuous central vacuole, and increased cellular organelles such as mitochondria and dictyosomes. There were two types of nuclear states of plasmodium, uninucleate and multinucleate. Both plasmodia were structurally similar, filled with lipid droplets, bounded with envelope, and containing mitochondria, endo-plasmic reticulum, and sometimes small vacuoles. Plasmodial fragmentation, which may be regarded as a way to discharge plasmodial materials into host cytoplasm, commonly occurred, forming plasmodial fragments by outgrowth of plasmodial cytoplasm and regional compartmentalization. Plasmodial fragments were degenerated sometimes followed by forming chains of spherical vesicles especially in the uninucleate plasmodial state. These ultrastructural features indicate the biotrophic nature of the pathogen associated with its pathogenesis in the susceptible host.

• MALSORI
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• no.54
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• pp.27-43
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• 2005

Existing standard speech coders can provide speech communication of high quality while they degrade the performance of speech recognition systems that use the reconstructed speech by the coders. The main cause of the degradation is that the spectral envelope parameters in speech coding are optimized to speech quality rather than to the performance of speech recognition. For example, mel-frequency cepstral coefficient (MFCC) is generally known to provide better speech recognition performance than linear prediction coefficient (LPC) that is a typical parameter set in speech coding. In this paper, we propose a speech coder using MFCC instead of LPC to improve the performance of a server-based speech recognition system in network environments. However, the main drawback of using MFCC is to develop the efficient MFCC quantization with a low-bit rate. First, we explore the interframe correlation of MFCCs, which results in the predictive quantization of MFCC. Second, a safety-net scheme is proposed to make the MFCC-based speech coder robust to channel error. As a result, we propose a 8.7 kbps MFCC-based CELP coder. It is shown from a PESQ test that the proposed speech coder has a comparable speech quality to 8 kbps G.729 while it is shown that the performance of speech recognition using the proposed speech coder is better than that using G.729.

• Journal of Power System Engineering
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• v.10 no.4
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• pp.119-126
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• 2006

Gerotor hydraulic motor is widely used in hydraulic systems due to its low speed, high torque output and compactness in rotational direct driving of a heavy weight. Gerotor is a Planar mechanism consisted of a pair of rotor and circular teeth of stator assembly which forms a closed space, so called a chamber. The motion of rotor relative to the circular tooth is produced by the pressure difference of hydraulic operating fluid between the adjacent chamber. As all active contact points of rotor and circular teeth are subjected to very high sliding friction, a reduction in the performance of the gerotor hydraulic motor can not be avoided. Therefore, the core design parameters of gerotor profile used in hydraulic motors is to minimize a friction force by high contact stresses. The analytical design method of gerotor profile, based on envelope of a family of curves, is proposed. In this study, the influence of the tip clearances on three critical contact points between rotor and circular teeth of stator assembly has been explored by experimental data in this paper. At the same time a improvement method to reduce the friction force is proposed and the tip clearances on three critical points for getting an optimum gerotor profile are also analyzed.

• Journal of Microbiology
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• v.35 no.3
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• pp.213-221
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• 1997

The secretion of the alkaliphilic Bacillus sp. S-1 extracellular pullulanase involves translocation across the cytoplasmic membrane of the Gram-positive bacterial cell envelope. Translocation of the intracellular pullulanase PUL-I, was traced to elucidate the mechanism and pathway of protein secretion from an alkaliphilic Bacillus sp. S-1. Pullulanase could be slowly bue quantitatively released into the medium during growth of the cells in medium contianing proteinase K. The released pullulanase lacked the N-terminal domain. The N-terminus is the sole membrane anchor in the pullulanase protein and was not affected by proteases, confirming that it is not exposed on the cell surface. Processing of a 180,000M$\_$r/ pullulanase to a 140,000M$\_$r/ polypeptide has been demonstrated in cell extracts using antibodies raised against 140,000M$\_$r/ extracellular form. Processing of the 180,000 M$\_$r/ protein occured during the preparation of extracts in an alkaline pH condition. A modified rapid extraction procedure suggested that the processing event also occured in vivo. Processing apparently increased the activity of pullulanase. The western blotting analysis with mouse anti-serum against 140-kDa extracellular pullulanase PUL-E showed that PUL-I is processed into PUL-X via intermediate form of PUL-E. Possible explanationa for the translocation are discussed.

• Biomolecules & Therapeutics
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• v.7 no.3
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• pp.216-220
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• 1999

A chemosensitivity assay with small replicate Mm5mt/cl C3H mammary tumor cell cultures was developed to determine whether changes in viral antigen expression and release into culture fluids could be utilized as an in vitro measure of modulating drug effect. The 52,000 MW viral envelope glycoprotein (gp52) of the mouse mammary tumor virus (MMTV) was measured in culture fluids of control and drug-treated cultures while cell density was simultaneously determined by cell staining and OD 664 nm determination. While extra-cellular gp52 levels and cell density progressively increased over 72 hours for control cultures, declines in both parameters provided dual measures of effect for combination [N(phophonacetyl-L-aspartic acid)+5-fluorouracil], combination 〔N(phophonacetyl-L-aspartic acid )+5-fluoro-5'-deoxyuridine〕and single component treatment of this combination. At each treated time point, thesecombinations begin to produce a greater decline in both cell density and gp52 levels as compared to single drug treatments. These results indicate that N(phopho-nacetyl-L-aspartic acid) in combination can enhance the effectiveness of single drug.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.12 no.7
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• pp.527-533
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• 2002

A railway vehicle should be satisfied with the safety criteria and ride comfort of passengers. A bogie of railway vehicle Is composed of many suspension components, such as springs, dampers and etc.. that have an influence on the dynamic behavior of the train wish the wheel/rail profiles and track geometries. Therefore, it Is necessary for engineers to check the Interference between vehicle limit and construction limit with considering the vehicle's behavior, because when the vehicle is running on curved track, it should be have enough clearance from infrastructure for safely, spacially In a subway system. This paper explains the effective method of analysis for vehicle limit considering the vehicle dynamic behavior and reviews the problem of vehicle limit for the Korean Standard Urban Train. The results show that the vehicle limit is over the construction limit when the Korean Standard Urban Train runs on the curved track with 180 m radius of curve.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.18 no.1
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• pp.47-56
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• 2008

This paper presents a detecting technique for the improvement in quality by appling the various vibrational characteristics theory. The object of this study is to objectively point out faulty gear by developing the program which can be used to analyze and predict the vibrational characteristics caused by gear wear, deformation and nick of auto-transmission. The fault detection methods by vibrational signal analysis of gear have been progressed in the various fields of industry. These methods have the advantage of being easy to attach the accelerometer without discontinuance of the structure. But not all the methods are efficient for finding early faults. So in the thesis, we completed development of the inspection system of vibration by appling the most efficient detecting methods and verified the system's reliability through experiments.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.18 no.11
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• pp.1143-1149
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• 2008

In a nuclear power plant, loose part monitoring and its diagnostic technique is one of the major issues for ensuring the structural integrity of the reactor system. Typically, accelerometers are mounted on the surface of a reactor vessel to localize impact location cavsed by the impact of metallic substances on the reactor system. However, in some cases, the number of the accelerometers is not enough to estimate the impact location precisely. In such a case, one of alternative plan is to utilize another type sensors that can measure the vibration of the reactor structure even though the measuring frequency ranges are different from each others. The AE sensors installed on the reactor structure can be utilized as additional sensors for loose part monitoring. In this paper, we proposed a new method to estimate impact location by using both accelerometer signal and AE signal, simultaneously. The feasibility of the proposed method is verified by an experiment. The experimental results demonstrate that we can enhance the reliability and precision of the loose part monitoring.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2004.10a
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• pp.47-52
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• 2004

In the design of scramjet intake for hypersonic flight, a variety of aerothermodynamics phenomena are encountered. These phenomena include blunt leading - edge effects, boundary layer development issues, transition, inviscid / viscous coupling, shock - shock interactions, shock / boundary - layer interactions, and flow profile effects. For intakes that are designed to operate within a narrow Mach number / altitude envelope, an understanding of a few of these phenomena might be required. In this work several predominant flowfield phenomena (viscous phenomena, boundary - layer separation, and combustor entrance profile) are discussed to investigate the performance of the intake at the altitude and angle of attack extremes of the HyShot flight experiment.

• Journal of the Korean Society of Propulsion Engineers
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• v.9 no.2
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• pp.89-96
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• 2005

This study has been performed on the mechanism of heat transfer between stratospheric airship and its surroundings while the airship is staying in the air at the altitude of 20km. The computational grid of airship has been generated and the results influenced by the number and the shape of grids have been compared. The temperature distributions have been obtained through this thermal analysis considering three modes of heat transfer - conduction, convection and radiation - in stratospheric conditions. Based on the airship's surface and inner temperature variations, the influence of temperature distributions on the helium envelope and the payload has been predicted.