• Journal of Korea Water Resources Association
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• v.52 no.spc2
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• pp.811-822
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• 2019

Environmental flows in the downstream sections of Yongdam Dam, Wonju Stream Dam, and Hongcheon River were estimated with selected target fish species such as Nigra for the site of Yongdam Dam, Splendidus for the site of Wonju Stream Dam, and Signifer for the site of Hongcheon River by considering endangered and domestic species. Physical habitat analysis was performed to estimate environmental flows for the study sites by applying the Physical Habitat Simulation (PHABSIM) and RIVER2D which combined hydraulic and habitat models. Based on the monitored data for ecological environment, the Habitat Suitability Index (HSI) for the target species was estimated by applying the Instream Flow and Aquatic Systems Group (IFASG). In particular, based on the result of fish monitoring, the HSI for each stage of the growth for target species was analyzed. As a result, the Weighted Usable Area (WUA) was maximized at $4.9m^3/s$ of flow discharge during spawning, $5.8m^3/s$ during the period of juvenile, and $8.9m^3/s$ during the adult fish season at the downstream section of Yongdam Dam. The result of the Wonju Stream Dam showed an optimal environmental flow of $0.4m^3/s$, $1.0m^3/s$, and $1.5m^3/s$ during the period of spawning, juvenile, and adult. The habitat analysis for the site of Hongcheon River, which is a non-regulated stream, produced an optimum environmental flow of $5m^3/s$ in the spawning period, $4m^3/s$ in the juvenile stage and $6m^3/s$ in the adult stage.

• Journal of Advanced Research in Ocean Engineering
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• v.1 no.4
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• pp.227-238
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• 2015

The present work was an attempt to investigate the applicability of truncated mooring systems to KRISO's deep ocean engineering basin (DOEB) with ratios of 1:100, 1:60, and 1:50. The depth of the DOEB is 15 m. Therefore, the corresponding truncated depths for this study were equal to 1500 m, 900 m, and 750 m. The investigation focused on both the static and dynamic characteristics of the mooring system. It was shown, in a static pull-out test, that the restoring force of a FPSO vessel could be modified to a good level of agreement for all three truncation cases. However, when the radius of the mooring site was reduced according to the truncation factor, the surge motion response during a free-decay test showed a significant difference from the full-depth model. However, the reduction of this discrepancy was achieved by increasing the radius up to its maximum possible value while considering the size of the DOEB. Especially, in terms of the time period, the difference was reduced from 24.0 to 5.3 s for a truncation ratio of 1:100, 54.1 to 8.6 s for a truncation ratio of 1:60, and 31.7 to 3.9 s for a truncation ratio of 1:50. As a result, the study verified the applicability of the truncated mooring system to the DOEB, and therefore it could represent the full-depth mooring system relatively well in terms of the static and dynamic conditions.

• Journal of Microbiology and Biotechnology
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• v.29 no.4
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• pp.577-586
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• 2019

The engineered Aspergillus oryzae has a high NADPH demand for xylose utilization and overproduction of target metabolites. Glucose-6-phosphate dehydrogenase (G6PDH, E.C. 1.1.1.49) is one of two key enzymes in the oxidative part of the pentose phosphate pathway, and is also the main enzyme involved in NADPH regeneration. The open reading frame and cDNA of the putative A. oryzae G6PDH (AoG6PDH) were obtained, followed by heterogeneous expression in Escherichia coli and purification as a his6-tagged protein. The purified protein was characterized to be in possession of G6PDH activity with a molecular mass of 118.0 kDa. The enzyme displayed maximal activity at pH 7.5 and the optimal temperature was $50^{\circ}C$. This enzyme also had a half-life of 33.3 min at $40^{\circ}C$. Kinetics assay showed that AoG6PDH was strictly dependent on $NADP^+$ ($K_m=6.3{\mu}M$, $k_{cat}=1000.0s^{-1}$, $k_{cat}/K_m=158.7s^{-1}{\cdot}{\mu}M^{-1}$) as cofactor. The $K_m$ and $k_{cat}/K_m$ values of glucose-6-phosphate were $109.7s^{-1}{\cdot}{\mu}M^{-1}$ and $9.1s^{-1}{\cdot}{\mu}M^{-1}$ respectively. Initial velocity and product inhibition analyses indicated the catalytic reaction followed a two-substrate, steady-state, ordered BiBi mechanism, where $NADP^+$ was the first substrate bound to the enzyme and NADPH was the second product released from the catalytic complex. The established kinetic model could be applied in further regulation of the pentose phosphate pathway and NADPH regeneration of A. oryzae to improve its xylose utilization and yields of valued metabolites.

• Journal of Soil and Groundwater Environment
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• v.28 no.1
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• pp.15-24
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• 2023

A persulfate(PS)/sulfidated microscale zero-valent iron(S-mZVI) system was tested for treating a soil contaminated with phenol. Sulfidation of bare mZVI was conducted using a mechanochemical process utilizing a ball mill in order to improve persulfate activation capacity and stability of unmodified mZVI. The synthesized S-mZVI performed markedly better than the bare mZVI in activating PS. The optimum molar ratio of sulfur to mZVI was around 0.12. In the soil slurry experiments, a very rapid and complete removal of phenol was observed at the optimum molar ratios of PS to S-mZVI of 2:1 and PS to phenol of 16:1. The phenol removal efficiencies decreased as the water content of the slurries decreased. This was believed to be due to increased soil oxidant demand as the amount of soil was increased as relative to the water content. To evaluate the field applicability of the process, slurry experiments adopting high soil contents were carried out that simulated in-situ soil mixing conditions. These experiments resulted in substantially compromised degradation efficiencies of 54.3% and 43.8% within 4 hours. The current study generally shows that the PS/S-mZVI process has a potential to be developed into a remediation technology for soils contaminated with organics.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.232-237
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• 2005

The cellulloytic enzymes, such as FPase and amylase, production by Trichoderma inhamatum KSJ1 using 10L air circulation bioreactor were tried. Physical properties of the employed bioreactor, mixing time, $k_{L}$ a were examined in airflow conditions of enzyme production cultures. Mixing time of distilled water at 0.6, 1.2, 1.8 vvm were 25, 21, 9 sec, respectively, and mandel's medium using rice straw and pulp as carbon sources were 31, 25, 15 sec. Mixing time decreased when airflow rate increased. $k_L$ a of distilled water at 0.6, 1.2, 1.8 vvm were measured -22.52, -29.31, -39.62, respectively, and the mandel's medium were -47.8, -18.5, -21.4. The $k_L$ a values was increased linearly as the air-flow rates increased. However the values in mandel's medium showed different results, especially at 0.6 vvm. Amylase and FPase activity at the 1.2 vvm was 3.39U/mL, 0.72U/mL in 5 days cultivation. and the activities at 0.6 vvm were 2.01U/mL, 0.39U/mL, respectively. The enzyme productivity at 1.2 vvm was higher than at 0.6 vvm, indicating that high oxygen tension is requested for the efficient enzyme production culture of T. inhamatum KSJ1.

• BMB Reports
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• v.28 no.1
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• pp.83-86
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• 1995

In the course of screening for new aminopeptidase M inhibitors which were expected to be analgesic, immunopotentiating, or anti-metastatic agents, the novel synthetic substance MR-387C[(2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl-L-valyl-L-prolyl-L-leucine] (M.W. 504 daltons) was obtained. It was competitive with the substrate and had an $IC_{50}$ value of $0.04\;{\mu}m/ml$ ($7.9{\times}10^{-8}\;M$) and an inhibition constant ($K_i$) of $3.8{\times}10^{-8}\;M$. This novel MR-387C was compared with various known inhibitors of aminopeptidase M. It inhibited the enzyme more strongly than any other microorganism-originated inhibitor, except probestin.

• Fire Science and Engineering
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• v.22 no.3
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• pp.188-193
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• 2008

Numerical simulation was carried out for a propane fire of mass transfer rate 3g/m^2-s$ on a 1m high vertical wall. The objectives of this study are to confirm the outcomes of evaluation of the simulator through simulation of natural convection, and to compare the results of the wall fire with those of previous studies. It was confirmed that the simulated boundary layer was laminar at C_s=0.2$ while it was turbulent at C_s=0.1$. The z direction velocity showed lack of turbulent mixing as seen in the natural convection case, and the profiles of temperature and velocities were in relatively good agreement with those of experiment and previous simulation. It was found that the air entrainment into the boundary layer was well predicted.

• Proceedings of the KIEE Conference
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• 1998.07d
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• pp.1379-1381
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• 1998

Powder electroluminescent device (PELD) structured conventionally dielectric and phosphor layer, between electrode and their layer fabricated by screen printing splaying or spin coating method. To promote performance of PELDs, we approached the experiments for different structure and thickness variation of PELD. Thickness variation($30{\mu}m{\sim}130{\mu}m$) was taken. To investigate electrical and optical properties of PELDs, EL spectrum, transferred charge density using Sawyer-Tower's circuit brightness was measured. Variation of structure in PELDs was as follows: WK-1 (ITO/BaTiO3/ZnS:Cu/Silver paste), WK-2 (ITO/BaTiO3/ZnS:Cu/BaTiO3/ZnS:Silver paste), WK-3 (ITO/BaTiO3/ZnS:Cu/BaTiO3/Silver paste), WK-4(ITO/BaTiO3+ZnS:Cu/Silver paste) As a result, structure of the highest brightness appeared WK-4 possessed 60 ${\mu}m$ thickness. The brightness was 2719 cd/$m^2$ at 100V, 400Hz.

• The Journal of Engineering Geology
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• v.24 no.2
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• pp.273-281
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• 2014

In most current seismic design codes, design earthquake ground motions are defined by a reference spectrum, based on bedrock and site amplification factors that quantify the geotechnical dynamic conditions. Earthquake engineering bedrock is the fundamental geotechnical formation where the seismic waves are attenuated without amplification. To better define bedrock in an earthquake engineering context, shear wave velocity ($V_S$ ) data obtained from in-situ seismic tests were examined for several rock strata in Korea; these data were categorized by borehole drilling investigations. The $V_S$ values for most soft rock data in Korea are > 750 m/s, which is the threshold $V_S$ value for identifying engineering bedrock from a strong motion station. Conversely, VS values are < 750 m/s for 60% of $V_S$ data in weathered rock in Korea. Thus, the soft (or harder) rock strata below the weathered rock layer in Korea can be regarded as earthquake engineering bedrock.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.19 no.10
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• pp.1075-1085
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• 2008

Recently, it is studied actively that DVB-S2 for mobility standard(DVB-S2M) is combined with DVB-H, DVB-T and conventional DVB-S2 standard for continuous satellite broadcasting and internet service. Especially, overcoming deep fading by tunnel is main subject. For overcoming deep fading, DVB-S2M proposed cross layer system is consist of upper layer and physical layer. Thus, this paper proposed optimal upper layer coding system fixing the physical layer coding under the simulation results by coding methods, train speed, data rate, interleaver size and IP packet size.