• Title/Summary/Keyword: Energy calibration

Search Result 428, Processing Time 0.028 seconds

Method for the Measurement of Dissolved Oxygen in a Cell Culture Microchannel Using Oxygen-Sensitive Luminescence (산소 민감 발광 염료를 이용한 마이크로 채널 내에서 배양되는 세포 주변의 산소 농도 측정)

  • Lee, Seung-Youl;Jin, Song-Wan
    • Transactions of the Korean Society of Mechanical Engineers B
    • /
    • v.36 no.5
    • /
    • pp.533-538
    • /
    • 2012
  • In this study, we used an $O_2$-sensitive luminescent dye to measure the $O_2$ concentration of culture media around HeLa cells cultured in a microchannel. $[Ru(bpy)_3]^{2+}$, which dissolves easily in water and which has no phototoxic effect, was used as the $O_2$-sensitive dye. The ratiometric sensing method was applied by introducing calcein as the $O_2$-insensitive dye, in order to overcome the disadvantages of intensity-based sensing. By performing calibration with an amperometric $O_2$ sensor, we could calculate the exact concentration of $O_2$ in the culture media. We applied this technique to measure the $O_2$ concentration around the cultured cells in the microchannel. As expected, the $O_2$ concentration gradually decreased as the cells moved farther away from the channel. This method is expected to be applicable to the investigation of hypoxia, which occurs commonly in scaffolds.

Cable-pulley brace to improve story drift distribution of MRFs with large openings

  • Zahrai, Seyed Mehdi;Mousavi, Seyed Amin
    • Steel and Composite Structures
    • /
    • v.21 no.4
    • /
    • pp.863-882
    • /
    • 2016
  • This study aims to introduce a new bracing system by which even super-wide frames with large openings can be braced. The proposed system, hereafter called Cable-Pulley Brace (CPB), is a tension-only bracing system with a rectilinear configuration. In CPB, a wire rope passes through a rectilinear path around the opening(s) and connects the lower corner of the frame to its opposite upper one. CPB is a secondary load resisting system with a nonlinear-elastic hysteretic behavior due to its initial pre-tension load. As a result, the required energy dissipation would be provided by the MRF itself, and the main intention of using CPB is to contribute to the initial and post-yield stiffness of the whole system. Using a stiffness calibration technique, optimum placement of the CPBs is discussed to yield a uniform displacement demand along the height of the structure. A displacement-based design procedure is proposed by which the MRF with CPB can be designed to achieve a uniform distribution of inter-story drifts with predefined values. Obtained results indicated that CPB leads to significant reductions in maximum and residual deformations of the MRF at the expense of minor increase in the maximum base shear and developed axial force demands in the columns. In the case of a typical 5-story residential building, compared to SMRF system, CPB system reduces maximum amounts of inter-story and residual drifts by 35% and 70%, respectively. Moreover, openings of the frame are not interrupted by the CPB. This is the most appealing feature of the proposed bracing system from architectural point of view.

Applicability Evaluation to Grid-based Rainfall-Runoff-Sediment Model for Sediment Discharge Estimation (격자기반 강우-유출-유사 모형의 유사량 산정에 관한 적용성 평가)

  • Choi, Hyun Gu;Park, Jun Hyung;Han, Kun Yeun
    • Journal of Wetlands Research
    • /
    • v.19 no.1
    • /
    • pp.132-143
    • /
    • 2017
  • It is essential to obtain periodic sediment discharge data in a river in order to minimize problems that may arise from the erosion, transport, and deposition of sediment. However, it is difficult to estimate sediment discharge by the sediment discharge measurement plan in Korea at present, and empirical fomulas or numerical models are used to replace them. This paper has applied the K-DRUM model, a grid-based rainfall-runoff-sediment model, to estimate sediment discharge and ensure the continuity of the data in the watershed. Discharge and sediment load in 17 watersheds were estimated and the applicability of the model was analyzed through comparisons with measured data. For quantitative evaluation, NSE, PBIAS and RSR items were used, and discharge results reflected the tendency of rainfall and showed high statistical value. In case of sediment discharge, the soil erosion process of the watershed is physically well reflected. When the calibration was performed using the measure data, the applicability seems to be excellent in estimating the continuous sediment discharge data in the real watershed.

Respiratory air flow measuring technique without sensing element on the flow stream (호흡경로 상에 감지소자가 없는 새로운 호흡기류 계측기술)

  • Lee, In-Kwang;Park, Jun-Oh;Lee, Su-Ok;Shin, Eun-Young;Kim, Kyung-Chun;Kim, Kyung-Ah;Cha, Eun-Jong
    • Journal of Sensor Science and Technology
    • /
    • v.18 no.4
    • /
    • pp.294-300
    • /
    • 2009
  • Cardiopulmonary resuscitation(CPR) is performed by artificial ventilation and thoracic compression for the patient under emergent situation to maintain at least the minimum level of respiration and blood circulation for life survival. Quality of the pre-hospital CPR not only significantly affects the patient's survival rate but also minimizes side effects caused by CPR. Good quality CPR requires monitoring respiration, however, traditional respiratory air flow transducers cannot be used because the transducer elements are located on the flow axis. The present study developed a new technique with no physical object on the flow stream but enabling the air flow measurement and easily incorporated with the CPR devices. A turbulence chamber was formed in the middle of the respiratory tube by locally enlarging the cross-sectional area where the flow related turbulence was generated inducing energy loss which was in turn converted into pressure difference. The turbulence chamber was simply an empty enlarged air space, thus no physical object was placed on the flow stream, but still the flow rate could be evaluated. Both inspiratory and expiratory flows were obtained with symmetric measurement characteristics. Quadratic curve fitting provided excellent calibration formula with a correlation coefficient>0.999 (P<0.0001) and the mean relative error<1 %. The present results can be usefully applied to accurately monitor the air flow rate during CPR.

Radiant Energy Filtering to Enhance High Temperature Measurement by a Thermography System (고온 계측 열화상 시스템 구현을 위한 복사에너지 필터링 연구)

  • Yoon, Seok Tae;Cho, Yong Jin;Jung, Ho Seok
    • Journal of the Korean Society for Nondestructive Testing
    • /
    • v.36 no.6
    • /
    • pp.466-473
    • /
    • 2016
  • In a shipbuilding process, thermal damage to the ship structure at the rear end results from an excessive heat input and conduction during welding process. To prevent such damage, appropriate control of the heat input, based on welding temperature measurement, is required. For temperature measurement, contact and non-contact methods are available; the thermography system is a popular non-contact temperature measurement. When the intensity of radiation from a high-temperature object is excessive, however, detecting the sensors of ordinary thermography systems leads to an inability in measuring the temperature due to saturation. Hence, this study suggests use of a neutral density filter that prevents an excessive amount of radiation from being accumulated in a thermography system, and thus makes it possible to quantitatively measure an object's temperature as high as $3000^{\circ}C$.

Experimental Investigation of Horizontal Buoyant Discharges from a Rosette-type Riser Using LIF System

  • Kwon, Seok Jae;Seo, Il Won;Kim, Ho Jung
    • Proceedings of the Korea Water Resources Association Conference
    • /
    • 2004.05b
    • /
    • pp.463-467
    • /
    • 2004
  • Rosette-type diffusers with four-ports per riser are constructed in relatively shallow water in Korea. However, the trajectorial bending phenomena due to lower-pressure inside the surrounded buoyant jets on the riser was not considered in most models and was not observed without any experimental results. The buoyant jet behavior affected by the bending effect where there have been growing interests need to be verified experimentally and need to be preceded in the analysis of the characteristics of the buoyant jets oil a riser. The hydraulic model experiments have been carried out to investigate the characteristics of the behavior of horizontal buoyant jets discharged from a Rosette-type riser with four ports as well as single port over a certain range of the experimental conditions including initial momentum and initial buoyancy using LIF (Laser Induced Fluorescence) system to obtain concentration fields. The intensity of the fluorescent light in each pixel on the images obtained from LIF system with the tracer of Rhodamine H was converted to the local dye concentration with a set of calibration procedures to account for the non-uniform distribution of light intensity and the attenuation of light energy by water medium. The experimental results shows that the trajectories from Your ports tend to bend more and more to the inner side with the increase of the densimetric Froude number while the buoyant jet from a single port rises up without any bending phenomena. The previous models, VISJET and Seo et al. (2002), do not simulate the trajectories well except the region before the bending section. This study will focus on the analysis of the behavior of the buoyant jets for mainly a Rosette-type riser by conducting hydraulic model experiments using LIF system.

  • PDF

Estimation of Moisture Content in Comminuted Miscanthus based on the Intensity of Reflected Light

  • Cho, Yongjin;Lee, Dong Hoon
    • Journal of Biosystems Engineering
    • /
    • v.40 no.3
    • /
    • pp.296-304
    • /
    • 2015
  • Purpose: The balance between miscanthus production and its cost effectiveness depends greatly on its moisture content during post processing. The objective of this research was to measure the moisture content using a non-destructive and non-contact methodology for in situ applications. Methods: The moisture content of comminuted miscanthus was controlled using a closed chamber, a humidifier, a precision weigher, and a real-time monitoring software developed in this research. A CMOS sensor equipped with $50{\times}$ magnifier lens was used to capture magnified images of the conditioned materials with moisture content level from 5 to 30%. The hypothesis is that when light is incident on the comminuted particles in an inclined manner, higher moisture content results in light being reflected with a higher intensity. Results: A linear regression analysis for an initiative hypothesis based on general histogram analysis yielded insufficient correlations with low significance level (<0.31) for the determination coefficient. A significant relationship (94% confidence level) was determined at level 108 in a reverse accumulative histogram proposed based on a revised hypothesis. A linear regression model with the value at level 108 in the reverse accumulative histogram for a magnified image as the independent variable and the moisture content of comminuted miscanthus as the dependent variable was proposed as the estimation model. The calibrated linear regression model with a slope of 92.054 and an offset of 32.752 yielded 0.94 for the determination coefficient (RMSE = 0.2%). The validation test showed a significant relationship at the 74% confidence level with RMSE 6.4% (n = 36). Conclusions: To compensate the inconsistent significance between calibration and validation, an estimation model robust against various systematic interferences is necessary. The economic efficiency of miscanthus, which is a promising energy resource, can be improved by the real-time measurement of its crucial material properties.

Development of Fast Side-impact Sensing Algorithm (고속 측면 충돌 감지 알고리즘의 개발)

  • 박서욱;김현태
    • Transactions of the Korean Society of Automotive Engineers
    • /
    • v.8 no.3
    • /
    • pp.163-170
    • /
    • 2000
  • Accident statistics shows that the portion of fatal occupant injuries due to side impacts is considerably high. The side impact usually leads to a severe intrusion of side structure into the passenger compartment. Furthermore, the safety zone for the side impact is relatively small compared to the front impact. Those kinds of physics for side impact frequently result in a fatal injury for the occupant. Therefore, NHTSA and EEVC are trying to intensify the regulation for the occupant protection against side impact. Both the regulation and recent market trends are asking for an installation of side airbag. There are several types of system configuration for side impact sensing. In this paper, we adopt the acceleration-based remote sensing method for the side airbag control system. We mainly focus on the development of hardware and crash discrimination algorithm of remote sensing unit. The crash discrimination algorithm needs fast decision of airbag firing especially for high-speed side impact such as FMVSS 214 and EEVC tests. It is also required to distinguish between low-speed fire and no-fire events. The algorithm should have a sufficient safety margin against any misuse situation such as hammer blow, door slam, etc. This paper introduces several firing criteria such as acceleration. velocity and energy criteria that use physical value proportional to crash severity. We have made a simulation program by using Matlab/Simulink to implement the proposed algorithm. We have conducted an algorithm calibration by using real crash data for 2,500cc vehicle. The crash performance obtained by the simulation was verified through a pulse injection method. It turned out that the results satisfied the system requirements well.

  • PDF

Use of Near Infrared Spectroscopy in the Meat Industry

  • Akselsen, Thorvald M.
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
    • /
    • 2000.11a
    • /
    • pp.1-14
    • /
    • 2000
  • The Near Infrared region of the energy spectrum was first discovered by Hershel in the year 1800. The principles of NIR is based on light absorption of specific organic chemical bonds. The absorption at each wavelength is measured and a spectre is obtained. The spectre is then treated mathematically and with the absorption data is converted to absolute units via a calibration. In the last two decades it has developed dramatically. With the invention of computers and the ability to treat a large amount of data in a very short time the use of NIR for many different purposes has developed very fast. During the last decade with the aid of very powerful PC's the application of NIR technology has become even more widespread. Now or days development of very robust calibrations can be done in a relatively short time with a minimum of resources. The use of Near Infrared Spectroscopy (NIR) in the Meat industry is relatively new. The first installations were taken into operation in the 80ties. The Meat Industry in often referred to as rather conservative and slow to embrace new technologies, they stay with the old and proven methods. The first NIR instruments used by the Meat Industry, and most other industries, were multipurpose build, which means that the sample presentation was not well suited to this particular application, or many other applications for that sake. As the Meat Industry grows and develops to meet the demands of the modern markets, they realise the need for better control of processes and final products. From the early 90 ties and onward the demand for 'rear time' rapid results starts growing, and some suppliers of NIR instruments (and instruments based on other technologies, like X-ray) start to develop and manufacture instrumentation dedicated to the particular needs of the Meat Industry. Today it is estimated that there are approximately 2000 rapid instruments placed in the Meat industry world-wide. By far most of these are used as at-line or laboratory installations, but the trend and need is moving towards real on-line or in-line solutions. NIR is the most cost effective and reproducible analytical procedure available for the twenty first century.

  • PDF

A Study on p-y Curves for Nearshore Seabed of Jeju Island (제주 연근해 해저암반의 p-y 곡선 모델에 관한 연구)

  • Jang, Young-Eun;Lee, Joonyong;Cho, Samdeok;Yoo, Dong-Woo;Choi, Changho
    • Journal of the Korean GEO-environmental Society
    • /
    • v.13 no.6
    • /
    • pp.73-81
    • /
    • 2012
  • Safety issue for offshore wind-turbine foundation becomes a crucial factor as offshore wind turbines have been scaled up. Correspondingly, there is a demand to understand the effect of soil-structure interaction on to system behavior in geotechnical engineering point of view. The p-y curve method researched in past few decades is one of the most appropriate methodology to analyze the problem. In this study, recently proposed p-y curve models for various rocks are calibrated to analyze the engineering characteristics of seabed of Jeju Island where it is known to be most suitable area for offshore wind energy farm. Step by step calibration process for p-y models is presented. Analysis results show that subgrade reaction generally increases as closer to seabed. It is also shown that the behavioral characteristics of foundation reflect well rock properties in terms of resultant moment, shear force, etc.