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http://dx.doi.org/10.3795/KSME-B.2012.36.5.533

Method for the Measurement of Dissolved Oxygen in a Cell Culture Microchannel Using Oxygen-Sensitive Luminescence  

Lee, Seung-Youl (Dept. of Mechanical System Engineering,Graduate School of Knowledge-Based Technology and Energy, Korea Polytechnic Univ.)
Jin, Song-Wan (Dept. of Mechanical System Engineering, Korea Polytechnic Univ.)
Publication Information
Transactions of the Korean Society of Mechanical Engineers B / v.36, no.5, 2012 , pp. 533-538 More about this Journal
Abstract
In this study, we used an $O_2$-sensitive luminescent dye to measure the $O_2$ concentration of culture media around HeLa cells cultured in a microchannel. $[Ru(bpy)_3]^{2+}$, which dissolves easily in water and which has no phototoxic effect, was used as the $O_2$-sensitive dye. The ratiometric sensing method was applied by introducing calcein as the $O_2$-insensitive dye, in order to overcome the disadvantages of intensity-based sensing. By performing calibration with an amperometric $O_2$ sensor, we could calculate the exact concentration of $O_2$ in the culture media. We applied this technique to measure the $O_2$ concentration around the cultured cells in the microchannel. As expected, the $O_2$ concentration gradually decreased as the cells moved farther away from the channel. This method is expected to be applicable to the investigation of hypoxia, which occurs commonly in scaffolds.
Keywords
Oxygen Sensitive Luminescent Dye; Tris(Bipyridine)Ruthenium(II)(Tris(Bipyridine) Ruthenium(II)); Calcein; Dissolved Oxygen Concentration; Hypoxia;
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