• BMB Reports
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• v.34 no.6
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• pp.551-558
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• 2001

We extended the measurable time scale of DNA dynamics to submicrosecond using a long-lifetime metal-ligand complex, $[Ru(phen)_2(dppz)]^{2+}$ (phen=1,10-phenanthroline, dppz=dipyrido[3,2-a:2',3'-c]phenazine) (RuPD), which displays a mean lifetime near 350 ns. We partially characterized the fluorescence resonance energy transfer (FRET) in calf thymus DNA from RuPD to nile blue (NB) using frequency-domain fluorometry with a high-intensity, blue light-emitting diode (LED) as the modulated light source. There was a significant overlap of the emission spectrum of the donor RuPD with the absorption spectrum of the acceptor NB. The F$\ddot{o}$rster distance ($R_0$) that was calculated from the spectral overlap was $33.4\;{\AA}$. We observed dramatic decreases in the steady-state fluorescence intensities of RuPD when the NB concentration was increased. The intensity decays of RuPD were matched the closest by a triple exponential decay. The mean decay time of RuPD in the absence of the acceptor NB was 350.7 ns. In a concentration-dependent manner, RuPD showed rapid intensity decay times upon adding NB. The mean decay time decreased to 184.6 ns at $100\;{\mu}M$ NB. The FRET efficiency values that are calculated from the mean decay times increased from 0.107 at $20\;{\mu}M$ NB to 0.474 at $100\;{\mu}M$ NB concentration. The use of FRET with a long-lifetime metal-ligand complex donor is expected to offer the opportunity to increase the information about the structure and dynamics of nucleic acids.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2009.11a
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• pp.89-94
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• 2009

The purpose of this study is to provide a fundamental data for reducing energy consumption and greenhouse gas emission of detached houses by investigating the energy consumption characteristic of detached houses in Daegu. Although the ratio of the detached houses decreases, the detached houses are common dwelling form next to apartments. Nevertheless the study about the energy consumption of detached houses has been insufficient compared apartments. There is a necessity which will investigate the energy consumption characteristic of detached houses. Because that with the building quality which is various form is different from apartments. This study investigate construction and equipment conditions and analyzing effective factors on energy consumption of detached houses. And this study draw up the energy consumption unit and emission factors unit for greenhouse gas of detached houses. This study represent a basic report for energy consumption reduction and helps effective use of energy.

• Environmental and Resource Economics Review
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• v.20 no.1
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• pp.99-118
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• 2011

'Low Carbon, Green Growth' may be the achievable target in industry section, depending on whether less reliance on fossil-fuels use can bring higher productivity growth in the long run. This paper tests for the short-run and long-run effects of investment on energy-saving equipments on productivity growth in the Korean manufacturing industries. The investment in energy efficiency causes an increase in costs (measurement effect) in the short-run, but in the long-run likely improve energy intensity and reduce costs (positive real effect) despite the delay in new other investment for technical innovation (negative real effect). A 2SLS regression was attempted to deal with endogeneity of energy-saving investment. The productivity effects were tested for five manufacturing sub-industries showing relatively high energy intensity with annual time series data from 1982 through 2006. No productivity effects were accepted for all five sub-industries except Chemical products. Positive real effect was considered to be exceeded by negative real effect, resulting in decreased productivity growth for Chemical products.

• Atmosphere
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• v.31 no.2
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• pp.143-156
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• 2021

This study investigates the impacts of land cover change due to urbanization on the Urban Heat Island Intensity (UHII) and the Heat Index (HI) over the Seoul metropolitan area using the Unified Model (UM) with the Met Office Reading Urban Surface Exchange Scheme (MORUSES) during the heat wave from 16, July to 5, August 2018. Two simulations are performed with the late 1980s land-use (EXP1980) and the late 2000s land-use (EXP2000). EXP2000 is verified using Automatic Weather Station (AWS) data from 85 points in the study area, and observation sites are classified into two categories according to the urban fraction change over 20 years; Category A is 0.2 or less increase, and Category B is 0.2 or more increase. The 1.5-m temperature and relative humidity in Category B increase by up to 1.1℃ and decreased by 7% at 1900 LST and 2000 LST, respectively. This means that the effect of the urban fraction changes is higher at night. UHII increases by up to 0.3℃ in Category A and 1.3℃ in Category B at 1900 LST. Analysis of the surface energy balance shows that the heat store for a short time during the daytime and release at nighttime with upward sensible heat flux. As a result of the HI, there is no significant difference between the two experiments during the daytime, but it increases 1.6℃ in category B during the nighttime (2200 LST). The results indicate that the urbanization increase both UHII, and HI, but the times of maximum difference between EXP1980 and EXP2000 are different.

• Journal of Energy Engineering
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• v.14 no.3 s.43
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• pp.180-186
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• 2005

As an engine has a wide range of RPM $(3000\~12000\;RPM)$, variable control system is necessary in order to increase engine performance. SCV has been frequently referred to as a possible way to increase engine performance at low RPM. The purpose of this study is to investigate on the influence of SCV, specially at the range of lower revolution, in a high-speed small engine. Experiments were conducted on 4 Valves SOHC/air-cooling single cylinder engine and SCV shapes have been tested fur swirl intensity, the performance of power, fuel consumption and emission. As a result, we find to use SCV range be below 5000 RPM which fuel consumption decreased $9\%$.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2007.04a
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• pp.349-352
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• 2007

In this paper, we describe the modeling of ablation based laser applications for innovative use in the military In the laser ignition system, a metal confinement is ablated with the high intensity pulsed energy, triggering a thermal ignition of the confined high explosives. The constitutive equations for the laser source, deformation of metals, and explosion of energetic materials are described.

• Proceedings of the KSME Conference
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• 2001.06e
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• pp.702-707
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• 2001

The characteristics of flow in dividing regions are precise, therefore their classification is very important not only in industry but also in hydrodynamics. By now, many studies of flow in dividing regions have been performed, but flow characteristics that use visualization in dividing regions have not been studied. The present study of the PIV and the CFD exhibit average velocity distributions, kinetic energy distributions and total pressure distributions etc of the total flow field due to the development of the accurate visualization optical laser and of optical equipment. Also, PIV is accurate with the flows characteristics of the dividing region as continuous analysis is done using input equipment. The study analyzes average velocity vector field, average kinetic energy, x-axis stress distributions, average and total pressure distributions of dividing regions with flow for visualization of the PIV and the CFD measurement in a dividing rectangular duct.

• 한국신재생에너지학회:학술대회논문집
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• 2008.10a
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• pp.375-378
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• 2008

This paper aims at identifying the factors that have influenced changes in the level of industrial $CO_2$ emissions. By means of complete decomposition method the observed changes are analyzed into five different factors: output level, energy intensity, energy mix and structural change and utility use. The application study refers to the manufacturing sectors in Korea. Moreover, this paper discusses the relationship between Korea's manufacturing $CO_2$ emission and economic growth (as measured by GDP), investigating whether economic growth is decoupling from $CO_2$ emission.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.66 no.2
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• pp.347-353
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• 2017

In order to prohibit global warming, various kinds of regulatory policies have been established in the whole world. One example is the establishment of the Renewable Portfolio Standard. It requires the increased portion in energy production from renewable energy sources. The Republic of Korea adopted the act on the promotion of the development, use, and diffusion of new and renewable energy since 2012. However, in spite of the effort on the consideration of the renewable energy sources, it was reported the carbon intensity of electricity in Korea was not that low in 2015. Thus, it is required to examine the recent state of the utilization degree of the renewable energy sources in Korea. This paper analyzed the statistical data provided by Korea Power Exchange (KPX) to examine any problems and solutions for generating electricity from the renewable energy sources. We focused on the generation capacity provided by the power plants participated in the market, the electric power trading amount, and the utilization coefficient for 10 years. By analyzing the data, we provide an alternative to solve some imbalance among the factors contributing to renewable energy use.

• Membrane and Water Treatment
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• v.10 no.1
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• pp.29-38
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• 2019

Microbial community and composition in stormwater runoff from mixed land use land cover (LULC) catchment with ongoing land development was diverse across the hydrological stage due different environmental parameters (hydrometeorological and physicochemical) and source of runoff. However, limited studies have been made for bacterial composition in this catchment. Therefore, this study aims to: (1) quantify the concentration of fecal indicator bacteria (FIB), stormwater quality and bacterial composition and structure according to hydrological stage; and (2) determine their correlation to environmental parameters. The 454 pyrosequencing was used to determine the bacterial community and composition; while Pearson's correlation was used to determine the correlation among parameters-FIB, stormwater quality, bacterial composition and structure-to environmental parameters. Results demonstrated that the initial and peak runoff has the highest concentration of FIB, stormwater quality and bacterial composition and structure. Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes were dominant bacteria identified in this catchment. Furthermore, the 20 most abundant genera were correlated with runoff duration, average rainfall intensity, runoff volume, runoff flow, temperature, pH, organic matter, nutrients, TSS and turbidity. An increase of FIB and stormwater quality concentration, diversity and richness of bacterial composition and structure in this study was possibly due to leakage from septic tanks, cesspools and latrines; feces of domestic and wild animals; and runoff from forest, destroyed septic system in land development site and urban LULC. Overall, this study will provide an evidence of hydrological stage impacts on the runoff microbiome environment and public health perspective.