• Journal of Ginseng Research
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• 제10권2호
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• pp.187-192
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• 1986

Activity of endogenous growth regulator in 4th year Panax ginseng root was investigated by second leaf sheath test of rice seedling and paper chromatogram of a acidic fraction of methanol extract before (March 28) and after (May 9) emergence of root bud, at the late season (Sept.4) and after leaf fall (November 11). GA$_3$ and ABA were used as reference. According to paper and high performance liquid chromatography of samples and authentic growth regulators the presence of insole acetic acid (IAA), gibberellic acid (GA$_3$) and abscisic acid (cis and trans ABA) was confirmed. These three regulators appeared to consist of major system though the existence of other regulators could not be ruled out. IAA activity seemed little changed through out the seasons. GA activity decreased in the later stages while ABA activity increased.

• Molecules and Cells
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• 제21권2호
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• pp.224-228
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• 2006

Glypican-3 (GPC3) is a member of the glypican family, which encodes cell-surface heparan-sulfate proteoglycans, and is frequently upregulated in hepatocellular carcinoma (HCC). We have recently reported that blocking endogenous GPC3 expression promotes the growth of HCC cell lines, suggesting that GPC3 plays a negative role in HCC cell proliferation. Here, we report that forced expression of GPC3 reduced the growth of HCC cells. We also found that FGF2-mediated cell proliferation was inhibited by GPC3. In addition, we observed that the adhesion of HCC cells to collagen type I and fibronectin was decreased by GPC3, whereas cellular migration and invasiveness were stimulated. Collectively, these results suggest that progression of hepatocellular carcinoma is associated with upregulation of GPC3.

• Journal of Plant Biotechnology
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• 제32권2호
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• pp.145-149
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• 2005

본 연구는 나도풍란의 기내 배양에서 몇 가지 종류의 식물생장억제제의 농도별 처리가 식물체 내 유리당 및 단백질 함량에 미치는 영향을 알아보기 위하여 실시되었다. 잎의 유리당의 함량은 생장억제제의 처리 농도가 높아짐에 따라 감소하는 경향이었고, 분석된 유리당 중 glucose의 함량이 가장 높았다. 뿌리의 유리당 함량은 잎과는 반대로 생장억제제 처리 농도가 높아짐에 따라 함량도 같이 높아지는 경향이었으며, 조사된 유리당 중 sucrose의 함량이 비교적 가장 높았다. 잎의 단백질 함량은 생장억제제 처리농도가 높아짐에 따라 감소하는 경향이었으나, 뿌리에서는 증가하는 경향을 나타내었다.

• 지역사회간호학회지
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• 제30권3호
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• pp.345-356
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• 2019

Purpose: The purpose of this study is to construct and test a structural equation model of posttraumatic growth (PTG) of earthquake victims based on Tedeschi and Calhoun's model (2004). Methods: Data were collected from 195 earthquake victims living in K. City. The exogenous variables include distress perception, resilience, and social support, and the endogenous variables include intrusive rumination, deliberate rumination, and posttraumatic growth. For data analysis, descriptive statistics, factor analysis, and structural equation modeling were performed. Results: The modified model showed a good fitness to the data. Moreover, 6 of the 9 paths of the final model were statistically significant, which include PTG affected by deliberate rumination (${\beta}=.58$, p<.001), resilience (${\gamma}=.18$, p=.001), and distress perception (${\gamma}=.20$, p=.002). These predictors explain 51.8% of variance in posttraumatic growth. Conclusion: Based on the results of this study, it is necessary to develop and disseminate preventive intervention programs to increase the resilience of earthquake-prone communities. In addition, after exposure to a community-scale traumatic event such as earthquake, we should provide social supports to alleviate distress perception and transition from intrusive rumination to deliberate rumination so that we can seek new meaning from the earthquake and facilitate posttraumatic growth.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.206-206
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• 2005

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.309.1-309.1
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• 2002

Ginsan, a polysaccharide extracted from Panax ginseng. was earlier scrutinized for a biological-response modifier. We further studied the protective and restorative activity of Ginsan against sublethal dose irradiation owing to increase production of endogenous hematopoietic growth factors such as IL-1. TNF-${\alpha}$. IL-6, GM-CSF. Which induce strong redox-emzyme elevation. Exposing to radiation induces reactive oxygen species (ROS). which play an important causative role in radiation damage. (omitted)

• Biomolecules & Therapeutics
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• 제25권4호
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• pp.396-403
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• 2017

Under normal, non-stressed conditions, intracellular p53 is continually ubiquitinated by MDM2 and targeted for degradation. However, in response to severe genotoxic stress, p53 protein levels are markedly increased and apoptotic cell death is triggered. Inhibiting the ubiquitination of p53 under conditions where DNA damage has occurred is therefore crucial for preventing the development of cancer, because if cells with severely damaged genomes are not removed from the population, uncontrolled growth can result. However, questions remain about the cellular mechanisms underlying the regulation of p53 stability. In this study, we show that p53-inducible gene 3 (PIG3), which is a transcriptional target of p53, regulates p53 stability. Overexpression of PIG3 stabilized both endogenous and transfected wild-type p53, whereas a knockdown of PIG3 lead to a reduction in both endogenous and UV-induced p53 levels in p53-proficient human cancer cells. Using both in vivo and in vitro ubiquitination assays, we found that PIG3 suppressed both ubiquitination- and MDM2-dependent proteasomal degradation of p53. Notably, we demonstrate that PIG3 interacts directly with MDM2 and promoted MDM2 ubiquitination. Moreover, elimination of endogenous PIG3 in p53-proficient HCT116 cells decreased p53 phosphorylation in response to UV irradiation. These results suggest an important role for PIG3 in regulating intracellular p53 levels through the inhibition of p53 ubiquitination.

• Fisheries and Aquatic Sciences
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• 제1권2호
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• pp.269-275
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• 1998

Spider crabs, Libinia emarginata, were eyestalk-ablated unilaterally and bilaterally to manipulate endogenous methyl farnesoate (MF) to increase during the embryogenesis. Endogenous MF were measured weekly over the embryogenesis of the crab, using HPLC with the aids of GC/MS and MS database (CAS 010485-70-8) for the identification of the hormone. Initial MF titers both in the hemolymph and embryos of intact control were at bottom levels and the hormone concentrations kept unchanged (p<0.05), reflecting physiological unnecessaries of the hormone in the embryogenesis. Eyestalk ablation significantly stimulated the crabs to increase endogenous MF in both tissues (p<0.0l). In the response of the embryos to the increased MF, no growth stimulations were observed, at least, in the first part of embryogenesis. The increased mortalities and immature sheddings of embryos resulted from the crabs under the influence of elevated MF in both tissues, instead, suggesting that the elevated MF against the crab's requirement blocked the normal developmental process of the crab embryos. These data can give crustacean endocrinologists some insights to understand the effects of the hormone on the crustacean reproduction studied previously in which JH analogs ambiguously affected the crustacean reproduction depending on the reproductive stages. The data also can give shrimp aquaculturists some implication of a possible generation of unfavorable shrimp seeds attributed to elevated egg MF originated from their eyestalk-ablated mother shrimp.

• 한국지역지리학회지
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• 제22권2호
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• pp.397-410
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• 2016

본 연구는 국가주도형으로 조성된 대표적인 산업집적지인 구미 IT 클러스터의 내생적 발전 가능성을 탐색하기 위해서 2005년부터 추진되어 온 산업단지 혁신클러스터정책의 미니클러스터 사업에 대하여 심층 고찰한 것이다. 구미 IT 클러스터를 구성하고 있는 산 학 연 주체들로 구성된 미니클러스터들은 관 주도의 외생적 조직으로 형성되었으나, 점차 자생적 조직화 과정을 거치면서 자율적인 학습 커뮤니티 조직으로 진화되는 모습을 나타내고 있다. 이를 통해 국지적 학습과 지식 공유가 활성화될 수 있는 사회적 자본의 축적과 국지적 뿌리내림으로 참여기업들뿐만 아니라 지역 기업들의 경쟁력 강화에 기여한 것으로 평가된다. 이러한 성과는 정부주도형으로 형성된 외생적 산업집적지가 대 다수를 차지하고 있는 한국의 산업집적지 활성화 정책 수립에 있어서 중요한 시사점을 제공한다. 특히 본 연구 결과는 미니클러스터 사업과 같이 자생적인 학습 커뮤니티 조직이 활성화될 수 있는 소프트웨어 중심의 클러스터 사업이 일관성 있게 추진될 필요가 있음을 강조한다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권5호
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• pp.757-766
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• 2002

Growth hormone-releasing peptide-2 (GHRP-2, also named KP102) is a new hexapeptide of a series of synthetic growth hormone-releasing peptides (GHRPs) which stimulates the secretion of growth hormone (GH) in vitro and in vivo in several species including calf, sheep and pig. The GH-releasing activity of GHRP-2 is two to three times more effective than that of the original GHRP-6, and GHRP-1 in the rats and humans. To date, GHRP-2 seems to be the most potent member of the family of GHRPs. Since the GHRPs are short peptides (5-7 amino acid residues), they are synthesized easily and are not as readily degraded in plasma as GHreleasing hormone (GHRH). These features ameliorate their potential on domestic animals because of their chemical nature the GHRPs are efficacious when administered i.v. orally or orally. However, studies in cow, pig and sheep do not indicate such a close relationship between GHRH, somatostatin (SS) and GH, calling into question the general applicability of the human and rat models. Perhaps there is an important role for an endogenous GHRP in the regulation of GH secretion in domestic animals. This review provides an overview on the current knowledge of physiological role of GHRP-2 in domestic animals.