• Journal of Species Research
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• v.10 no.2
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• pp.154-158
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• 2021

An endemic endangered species, Aster altaicus var. uchiyamae (Danyang aster) B2015-0044, is cultivated at the Shingu Botanical Garden, which serves as the ex situ conservation institution for this species. In this work, we sequenced the chloroplast genome of A. altaicus var. uchiyamae B2015-0044. We found that the chloroplast (cp) genome of B2015-0044 was 152,457 base pairs(bps) in size: 84,247 bps of large single copy regions(LSC), 25,007 bps of inverted repeats(IRs), and 18,196 bps of small single copy regions. The B2015-0044 cp genome contains 79 protein-coding genes (PCGs), 4 RNA genes, 29 tRNA genes, and 3 pseudogenes. These results were identical to a previously reported cp genome (Park et al., 2017), except for two sites in introns and three in intergenic spacer (IGS) regions. For the intronic differences, we found that clpP.i1 had a 1-bp small simple repeat (SSR) (T) and petD.i had a 3-bp SSR (ATT). We found 1-bp SSRs in the IGSs of trnT_ggu~psbD and psbZ~trnG_gcc, C and A, respectively. The IGS of(ndhF)~rpl32 had a SNP. Based on our results, the cp genome of the A. altaicus var. uchiyamae can be classified into two genotypes, [C]¹-[A]¹²-[T]¹²-[ATT]⁴-C and [C]²-[A]¹¹-[T]¹¹-[ATT]²-A.

• Plant Biotechnology Reports
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• v.2 no.3
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• pp.213-218
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• 2008

Swertia chirata is an endangered gentian species that prefers to grow at higher altitudes. This ethnomedicinal herb is known primarily for its bitter taste caused by the presence of important phytochemicals that are directly associated with human health benefits. Due to a continuous loss of habitat and inherent problems of seed viability and seed germination, alternative strategies for propagation and conservation are urgently required to prevent the possible extinction of this species. We have formulated a reproducible protocol for the rapid propagation and conservation of this plant using leaves taken from in vitro shoot cultures. Direct induction of more than seven shoot buds per explant was achieved for the first time when the explants were placed on MS medium supplemented with $2.22{\mu}M$ N-6-benzyladenine, $11.6{\mu}M$ kinetin, and $0.5{\mu}M$ ${\alpha}-naphthalene$ acetic acid. Direct organogenesis was noted exclusively from the adaxial surface of the basal segments of leaves. Leaves closer to the apical meristem were more responsive than those farther away from the meristem. Plants raised through direct organogenesis were evaluated for their clonal fidelity by chromosomal analysis and DNA fingerprinting. Complete plants were successfully transferred to the field condition and produced viable seeds. Given the enormous potential of this age-old medicinal plant in terms of potential health-benefitting drugs, this protocol can be used for commercial propagation purposes and to initiate future genetic improvement studies.

• Korean Journal of Plant Resources
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• v.35 no.2
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• pp.317-345
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• 2022

This study carried out a survey to utilize basic data for the conservation of northern lineage plants that are vulnerable to climate change, targeting algific talus slope of a specific forest biodiversity region in Yeotan-ri, Jeongseon-eup, Jeongseon-gun, Gangwon-do. The survey was conducted by dividing the wind hole into 625 m² inside and 6,925 m² outside based on the wind hole. The vascular flora were identified as 84 families, 203 genera, 296 species, 6 subs., 27 var., 2 for., and a total of 331 taxa. This was found to be about 7% of 4,724 species of vascular plants in Korea. Plants endemic to the Korean peninsula were identified as 19 taxa, including Syringa fauriei, and 11 taxa for rare plants designated by IUCN. Critically endangered grade (CR) was Paeonia obovata 1 taxa, Endangered grade (EN) was Forsythia saxatilis and Prunus choreiana 2 taxa. A total of 78 taxa were florisitc target species, and 19 taxa were classified for indicator plants in the limestone area. As for alien plants, 20 taxa such as Taraxacum officinale were identified, and the naturalization rate was 6.04% and the urbanization rate was 5.33%. Geographical plants of the study area are 2 taxa of Syringa fauriei and Syringa villosa subsp. wolfii.

• Korean Journal of Environment and Ecology
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• v.7 no.2
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• pp.112-117
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• 1994

The present conservation status on the rare and endangered plants in Tokyusan National Park were surveyed and re-evaluated by the new criteria which prepared by I.U.C.N. The species such as Lonicera vidalii, Clematis chiisanensis, Cypripedium macranthum and Allium taquetii were grouped as Critical, Eranthis stellata, Abies koreana, Taxus cuspidata, Tricyrtis dilatata, Paeonia japonaca, Stewartia koreana, Rhododendron tschnoskii, Buplerum euphorbioides, Lilium cernum and Oreorchis patens were grouped as Endangered, Adonis amurenis and Disporum ovale were grouped as Vulnerable. The above mentioned species were mainly located at the vicinity of mountain trails and projected developing sites. The potentials for the habitat destructions, mainly, due to human activities are expected to be serious in the future unless the proper management plans prepared for the specific plants and the specific habitats.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.630-636
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• 2010

A survey of the endangered orchid Orchis militaris populations was carried out in north-eastern Italy. The occurrence of fungal root endophytes was investigated by light and electron microscopies and molecular techniques. Two main sites of presence were individuated in the Euganean Hills, differing as to the percentage of flowering individuals and of capsules completing maturity. Fluorescence microscopy revealed an intracellular cortical colonization by hyphal pelotons. Two ITS PCR products co-amplified. Sequencing revealed for the former an identity and a high similarity (99%) with a Tulasnellaceae (Basidiomycota) fungus found within tissues of the same host in independent studies in Hungary and Estonia, suggesting an interesting case of tight specificity throughout the Eurosiberian home range. The second amplicon had 99% similarity with Tetracladium species (Ascomycota) recently demonstrated as potential endophytes. TEM revealed two different hyphal structures. Double fungal colonization appears to occur in Orchis militaris and the possible requirement of a specific fungal partner throws light on the causes of this plant's rarity and threatened status.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.6
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• pp.18-35
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• 2009

Distribution of vascular plants in Yangyang-gun were investigated 12 times from Apr. to Sept., 2008. In the result of survey, the flora of this area consist of 412 taxa totally; 89 families, 259 genera, 366 species, 5 subspecies, 37 varieties and 4 forms. There were remarkable plants such as 11 taxa of Korean endemic plants, 58 taxa of specific plants which were designated by the Ministry of Environment, 2 taxa of endangered plants of Ministry of Environment, 7 taxa of rare, endangered plants of Korea Forest Service and 28 taxa of afforestation plants and 296 taxa of useful plants. Furthermore, 36 taxa of naturalized plants (8.74%) were also observed in this area. Vegetation of investigated area was conserved well. However, vegetation has been destroyed rapidly due to construction of commercial complex for human recreation such as condominiums and stores. Therefore, it is need to efficient management of the water quality and vegetation by conservation strategies from huge visitors.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.6
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• pp.57-69
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• 2011

The present paper deals with a research of life form and vegetation of the plants extant in the habitat of endangered Parnassius bremeri in Uiseong, Gyeongsangbuk-do with a view to restore their habitat and create substitute habitats. The research found that there are a total of 130 vascular plants including 47 families, 96 genera, 113 species, 1 subspecies and 16 varieties. In terms of their life form, hemicryptophytes (H) is the majority of dormancy form with 41 species or 31.54%, while $R_5$ is majority of radicoid form with 72 species or 55.38%, gravitational disseminule form ($D_4$) is majority among the disseminule forms with 68 species or 52.31% and erect type (e) is majority of growth form with 57 species or 43.85%. Major constituents of the habitat are Sedum kamtschaticum, Selaginella tamariscina, Orostachys japonica, Arundinella hirta, Cymbopogon tortilis var. goeringii, Themeda triandra var. japonica, Carex humilis, Indigofera kirilowii, Imperata cylindrica var. koenigii, Festuca ovina, Artemisia capillaris, etc., all of species which are in the initial stage of succession and show simultaneously features characteristic of vegetation on the rock. In the habitat 1. Sedum kamtschaticum- Selaginella tamariscina community and Pinus densiflora community are distributed whereas, in the habitat 2. Sedum kamtschaticum-Selaginella tamariscina community, Sedum kamtschaticum-Carex humilis community, Sedum kamtschaticum-Cymbopogon tortilis var. goeringii community and Sedum kamtschaticum-Imperata cylindrica var. koenigii community are growing and in the habitat 3. Sedum kamtschaticum-Selaginella tamariscina community, Selaginella tamariscina community, Sedum kamtschaticum-Indigofera kirilowii community and Quercus acutissima community are seen distributed there, too. Typical of food plants growing in the habitat is Sedum kamtschaticum, found mostly in the rocky area and its surroundings. Absorption plants are those that usually bloom in the season when imagoes emerge. From the research, it becomes evident that the blooming season of Sedum kamtschaticum, obviously a food plant, corresponds exactly to the emergence timing of imagoes. Cirsium japonicum var. ussuriense, Rosa multiflora, Erigeron annuus, Spiraea prunifolia var. simpliciflora are found to belong to these plants.

• Korean Journal of Environment and Ecology
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• v.25 no.4
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• pp.498-515
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• 2011

The flora of Juwangsan National Park was surveyed. The field works was done for seven times from March to October 2008. Endangered species designated by The Ministry of Environment in Juwangsan National Park were found to be Hylotelephium ussuriense (KOM.) H. OHBA, Berchemia berchemiaefolia (MAK.) KOIDZ, Jeffersonia dubia BENTH., Iris odaesanensis Y.N.LEE and Acanthopanax senticosus (RUPR. et MAX.) HARMS, Lilium cernum KOM., Thalictrum coreanum LEV.(7 taxa). The floristic special plants were recorded to a total of 88 taxa; that is, degree V species (8 family 9 taxa) were containing as Jeffersonia dubia BENTH., Berchemia berchemiaefolia (MAK.) KOIDZ, Lilium cernum KOM., etc, degree IV species (4 family 5 taxa) were Cimicifuga heracleifolia KOM., Anemone reflexa STEPH. et WILLD., Patrinia rupestris JUSS., etc, degree III species (16 family 18 taxa) were containing; Pyrrosia petiolosa (CHRIST.et BARONI) CHING, Exochorda serratifolia S. MOORE., Aristolochia manshuriensis KOM., etc, degree II species (14 family 18 taxa) were Paeonia japonica MIYABE et TAKEDA, Isopyrum raddeanum (REGEL) MAX., Cypripedium macramthum SW., etc and degree I species (30 family 38 taxa) were Botrychium virginianum (L.) SW., Chloranthus japonicus SIEB., Dictamnus dasycarpus TURCZ., etc. 15 taxa that included Salix hulteni FLODERUS, Celtis choseniana NAKAI, Clematis trichotoma NAKAI, Vicia venosissima NAKAI, etc. were found to be endemic to korea and 21 taxa that included ; Bilderdykia dumetora (L.) DUM., Bilderdykia convolvulus DUM., Lepidium apetalum WILLD., Amorpha fruticosa L. and Symphytum offcinale L., Hibiscus trionum L. etc. were identified as naturalized plants.

• Korean journal of applied entomology
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• v.60 no.4
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• pp.423-429
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• 2021

The ovipositional characteristics of the endangered species Prosopocoilus astacoides blanchardi was investigated for indoor mass-rearing. This insect required 3 weeks or more for mating after emergence when introduced at the 1:1 ratio of male to female. Among temperatures studied at 16, 20, 24, 28, and 32℃, the pre-ovipositional period of P. astacoides blanchardi was longest at 16℃ with an average of 109 days after emergence, while it was shortest at 32℃ with an average of 59.4 days. The number of eggs laid at 24℃ was highest with an average of 40.6 per female. The average lifespan of adults at 16℃ was 208.8 days for males and 263.9 days for females, while at 32℃ 96.1 days for males and 130.3 days for females. The number of laid eggs was dependent on insect rearing chamber size. The oviposition-trees did not significantly (P > 0.05) affect the number of laid eggs and ovipositional period. The results of this study could be used as rearing data for the restoration and mass propagation of the endangered species P. astacoides blanchardi.

• Korean Journal of Pharmacognosy
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• v.38 no.2 s.149
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• pp.157-163
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• 2007

Trillium tschonoskii Maxim. is a kind of 64 endangered plant species designated by the Ministry of Environment in Korea. It is very a rare native plant throughout the country. Therefore, this study was conducted to investigate the native T. tschonoskii in Ulleung Island for their distribution and ecological characteristics. They were mostly distributed valley, mid-slope of the mountain from 186 to 458 m altitude. They had a little low light conditions as they faced west, north west, south west, south-south west, north-north east and east-east north. They were provided with slightly humid condition. Aerial humidities of these quadrates were variable as 21.5-71%. They were located on the variable slopes of 5 to 43 degrees. Soil humidity was slightly dry. Native sites were semi-shaded because tree layer such as Tilia insularis, Zelkova serrata, okamotoanum etc.and sub-tall tree layer such as Ligustrum foliosum, Comellia japonica, Tilia insularis, Callicarpa japonica and so on were grown near by. Woody plants such as A. Okamotoanum, Sorbus commixta, Fagus japonica var. multinervis etc. were frequently observed. These environmental conditions were suitable that T. tschonoskii grows up naturally because of high Percentage as 52.78% of blooming individuals in this study. These native sites were classified with three communities, T. tschonoskii-Majanthemum dilatatum community, T. tschonoskii-Anthriscus sylvestris community and T. tschonoskii-Hepatica maxima community in accordance with altitude and aerial humidity. The best way ffr the preservation of T. tschonoskii habitats and maintainance of its recent population is maintained in recent environmental condition and ecological condition without any management by human-being.