• Journal of Plant Biotechnology
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• v.7 no.2
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• pp.135-141
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• 2005

Protocols for in vitro conservation was developed for Coleus forskohlii. Plants maintained both in field served as explant source. Shoot tips and single node cuttings were used to optimize protocols for in vitro multiplication. MS basal medium supplemented with $0.54\;{\mu}M$ naphthalene acetic acid (NAA) and $8.87\;{\mu}M$ benzy-ladenine (BA) induced multiple shoots in shoot tips and nodes. Shoot multiplication was amplified with a gradual decrease of BA concentration, leading to its final omission after 4 months. Concomitant rooting on multiplication media enabled successful establishment extra vitrum. For in vitro conservation studies, experiments were carried out with 2-3 week maintained in vitro plants under standard and reduced culture conditions (SCC, RCC). In vitro plants could be successfully conserved in full strength MS medium (FMS) under SCC for 6 months without subculture with full potential to regenerate, producing viable shoots and nodes. The root production remained unaffected due to conservation, showing high rooting activity in mannitol and low temperature treatments. Preset low temperature (15 and $10^{\circ}C$) and reduction in media constituents does not appear to favour conservation, although the former accomplished conservation levels equal to (FMS) under SCC.

• Korean Journal of Plant Resources
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• v.22 no.3
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• pp.248-265
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• 2009

This study was performed to investigate the flora and plant resources of Mt. Gamak (537.8 m) from April to October 2007. The collected vascular plants were composed of all 478 taxa including cultivated species, and classified into 410 species, 3 subspecies, 61 varieties, and 4 forms of 284 genera under 94 families. Six taxa of the Korean endemic plants and 4 taxa of the rare and endangered plants were also distributed in this mount. Resource plants were categorized into edible 222, pasturing 208, medicinal 183, stainable 134, ornamental 96, timber 20, fiber 6 and industrial 4 taxa, respectively. Floristic geography of the investigated area was regarded as the boundary between middle parts in floristic pattern of the Korean Peninsula.

• Korean Journal of Ecology and Environment
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• v.53 no.3
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• pp.221-228
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• 2020

We studied the development of the endangered gold-spotted pond frog, Pelophylax chosenicus eggs and analyzed growth and survival rate of the tadpoles while feeding different feed to obtain the basic data on the its biological traits and reproduction. We observed the fertilized eggs of gold-spotted pond frog that naturally spawned in a breeding tank using a microscope. Fertilized eggs were sphere shape surrounded by colloid layers, clearly distinct with animal and plant poles. The fertilized eggs hatched 31 hours after fertilization and operculum complete at 175 hours after fertilization at water temperature of 27.0±1.0℃. As a result of growth and survival rate by different feed from 35 days after mouth open stage, vegetable feed (VF) group and tetra-min (T) group were significantly higher average weight gain rate (WG)(5843.9% and 5736.3%, respectively) and average specific growth rate (SGR) (11.67% and 11.62, respectively) compared with other groups (WG: 641.8~4625.2%, SGR: 5.72~11.01%). The vegetable (V) group showed the lowest growth rate (P<0.05). The average feed efficiency was 177.83% in the VF group, about 17 times higher than the V group. In addition, the average survival rate of the VF and T groups were the high at 97.5% and 100%, respectively, and the V group was the lowest at 32.5%. Therefore, the more efficient feeds for successful breeding is singly with VF or T.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.16-16
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• 2018

Flora investigations have been conducted by many researchers for a long time in Korea. Even though large amount of investigation data has been accumulated, there is no accurate statistics or database because most of data were published in a printed form. We developed a web-based database of flora investigation, named as the Integrated Korean Flora Database (http://www.floradb.net/) to understand distribution patterns and habitats of plants in Korea. Till now, 480 published paper, 356 thesis, 76 reports and books, and 8 unpublished papers written in between 1962 and 2017 were collected and their species lists from 280 papers were parsed into the database. From 124,105 records, 3,100 species belonging to 206 families and 965 genera were identified via comparing with two major Korean plant species lists. 55 endangered species, 159 endemic species, and 367 rare species were identified. The most frequently surveyed species were Commelina communis in herbaceous and Rosa multiflora in woody plants. Microclimate data provided by Korea Meteorological Administration were also integrated and analyzed to assign cold hardness zones for each species. By comparing minimum temperature (<2%) acquired from automated weather stations (AWS) near by plant species, 6a to 10b zones (7b is the most frequent zone) were identified. Integrated Korean Flora Database will be a fundamental platform of korea flora investigation as well as a new standard for classifying distribution of plants based on accurate microclimate data. Moreover, it can also provide evidences of investigated plant species, such as specimen and/or pictures with connecting to the InfoBoss Cyber Herbarium (http://herbarium.infoboss. co.kr/) and Biodiversity Observation Datbase (BODB; http://www.biodiversitydb.org/).

• Korean Journal of Plant Taxonomy
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• v.37 no.1
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• pp.41-59
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• 2007

This study was to establish the floristic composition of northern region in Gyeonggi-Province area($126^{\circ}45^{\prime}-127^{\circ}37^{\prime}E$, $37^{\circ}31^{\prime}-38^{\circ}17^{\prime}N$). This work was investigated from April, 1988 to October, 2004. The flora of vascular plants of northern region in Gyeonggi-Province were consisted of total 2,030 taxa; 145 families, 656 genera, 1,611 species, 7 subspecies, 320 varieties, 1 subvarieties and 91 formae. Among them, Korean endemic species were composed of 46 families, 85 genera, 84 species, 35 varieties, 1 subvarieties, and 18 formae, total 138 taxa. The rare and endangered plants based on floral region indicated by Ministry of Environment were 416 taxa; V rank species 18 taxa, IV rank species 79 taxa, III rank species 111 taxa, II rank species 80 taxa, I rank species 128 taxa. Also the naturalized plants were 114 species, correspond to 39.7% of among total 287 species appeared in South Korea. the result of PCA, Gwangju mountain chain at northern region of Gyeonggi-Province has rich and diverse vascular plants, and there are many Korean endemic, rare and endangered plants. therefore Gwangju mountain chain must be the core axis in for conserving the plant biodiversity and richness.

• Journal of Ecology and Environment
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• v.42 no.1
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• pp.30-42
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• 2018

Background: The southern and eastern parts of the Gobi Desert area are a unique dry ecosystem with a diverse regional desert, semi-desert, and mountain dry steppe flora. This area habitat is located at the overlap of different floristic regions; on its northeast side, Central Asian desert flora is dominating, and on the eastern side, East Asian flora is observed. The comprehensive survey was carried out to find the floral diversity of the medicinal plants on the region. Methods: All recorded species in this study were based on the collected voucher specimens between June and August in the year 2017. Results: We recorded 23 families, 57 genera, and 78 species of vascular plants. The families Asteraceae (15 species), Fabaceae (10 species), and Amaranthaceae (10 species) were represented most in the study area, while Caragana (5 species), Salsola (4 species), and Arnebia (3 species) were the most common genera found. Conclusion: Conservation status for remarkable species was also reviewed based on the literature. Around the study area, 24 species as "sub-endemic," 10 species as "very rare," 4 species as "rare," 1 species as "alien," 13 species as "relict," 10 species as "Red Book," 2 species as "endangered (EN)," 3 species as "vulnerable (VU)," 3 species as "near threatened (NT)," and 2 species as "least concern (LC)" plants are growing.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.301-301
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.178-178
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• 2004

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.3
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• pp.83-98
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• 2018

This study was conducted on rare plants distributed in Chungnam area from April, 2012 to November, 2017. The rare plant populations that are emerging are: Utricularia pilosa, Jeffersonia dubia, Iris ruthenica, Tipularia japonica, Calanthe discolor, Parasenecio pseudotaimingasa, Ranunculus kazusensis, Berchemia racemosa, Pogonia minor and Glehnia littoralis. According to the criteria of the International Union for Conservation of Nature (IUCN), plant resources in the rare species category were one important type (CR), three hazardous species (EN) and six vulnerable species (VU). As a result of analyzing characteristics of ecological environment and threats, five types of habitat types, two aquatic areas, two types of flatland and coastal sand dune distribution types were analyzed. The decreasing tendency of the rare plant populations in the surveyed area is judged to be artificial disturbance and habitat destruction rather than climate or environmental change. Considering the characteristics of habitat, conservation measures should be prepared for each population.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.29-44
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• 2006

The flora of vascular plants in Geojedo revealed to be 550 taxa based on only the voucher specimens to be collected. It comprise 108 families, 341 genera, 478 species, 69 varieties and 3 forms respectively. Among them, 13 taxa of Korean endemics and 8 taxa of rare and endangered plants were investigated in addition to 75 taxa of specially designated plants announced by the Ministry of Environment. Furthermore, 36 taxa of induced and naturalized plants were also observed in this area. One of the characteristics of forest vegetation is the broadly occupation of Pinus thunbergii on whole area of Geojedo. Although it's well condition and conservation status, the destruction area of vegetation was rapidly increasing by the industrial development, the deforestation and the tramp as well as by the basic disturbances of human beings. Therefore, more active and positive conservation strategy such as expansion of conservation area should be prepared rapidly by the results of long-term monitoring studies.