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Purification and Characterization of the Recombinant Arabidopsis thaliana Acetolactate Synthase

  • 조규봉;홍성택;최명운;장수익;최정도;고은희
    • Bulletin of the Korean Chemical Society
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    • v.18 no.6
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    • pp.648-653
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    • 1997
  • Acetolactate synthase was purified from Escherichia coli MF2000/pTATX containing Arabidopsis thaliana acetolactate synthase gene. Purification steps included DEAE cellulose ion exchange column chromatography, phenyl sepharose hydrophobic column chromatography, hydroxylapatite affinity column chromatography, and Mono-Q HPLC. Molecular weight was estimated to be ∼65 KDa and purification fold was 109 times. The enzyme showed a pH optimum of 7 and the KM value was 5.9 mM. The purified enzyme was not inhibited by any of the end products, valine, leucine, and isoleucine.

A Study on the Utilization of Industrial Waste to Improve the Durability of Base Concrete (바탕콘크리트 내구성 향상을 위한 산업폐기물 활용에 관한 연구)

  • Kim, Dae-Geon
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2022.04a
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    • pp.107-108
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    • 2022
  • This study aims to solve environmental problems by reducing complex degradation and recycling industrial waste by utilizing waste fibers and blast furnace slags, which are industrial by-products. In addition, it is intended to secure long-term durability to reduce cracks. To this end, the disadvantages of fiber-reinforced concrete are to solve the problem of lowering liquidity and ensuring curing time, and to find the optimal combination when waste fibers and blast furnace slag are used together.

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The effect of consumers' self-regulatory modes and scarcity messages on fashion product evaluations (소비자의 조절모드와 희소성 메시지가 패션제품평가에 미치는 영향)

  • Baek, Sora;Hwang, Sunjin;Yeo, Junsang
    • The Research Journal of the Costume Culture
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    • v.22 no.1
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    • pp.99-111
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    • 2014
  • This study looked into the scarcity effect based on the consumers' self-regulatory modes and determined the effect that such a regulatory mode and that quantity-scarcity messages would have on evaluations of fashion products. To that end, the study used a 2 (regulatory mode: assessment mode/locomotion mode)×2 (message type: quantity-scarcity message/non-scarcity message) between-subjects factorial design in which buying impulsiveness was presented as a mediator variable that showed differences in product evaluations depending on the message type and on the presence of a self-regulatory mode. The results of this study showed that locomotion-oriented consumers evaluated products more positively compared to assessment-oriented consumers when the quantity-scarcity message was presented. When a non-scarcity message was presented, no significant difference was observed in product evaluations based on the consumers' self-regulatory modes. In addition, buying impulsiveness in consumers served a mediator role in the evaluations of fashion products. The results of this study imply that a clear understanding of consumers is a prerequisite for maximizing the scarcity effect and that companies need to differentiate in terms of the way they use scarcity messages based on consumers' self-regulatory modes.

INTRODUCTION TO THE COMS METEOROLOGICAL DATA PROCESSING SYSTEM

  • Ahn Myoung-Hwan;Seo Eun-Jin;Chung Chu-Yong;Sohn Byung-Ju;Suh Myoung-Seok;Oh Milim
    • Proceedings of the KSRS Conference
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    • 2005.10a
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    • pp.95-97
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    • 2005
  • Communication, Ocean, and Meteorological Satellite (COMS) to be launched in year 2008 will be the first Korean multi-purpose geostationary satellite aiming at three major missions, i.e.: communication, ocean, and meteorological applications. The development of systems for the meteorological mission sponsored by the Korea Meteorological Administration (KMA) consists of payloads, ground system, and data processing system. The program called COMS Meteorological Data Processing System (CMDPS) has been initiated for the development of data processing system. The primary objective ofCMDPS is to derive the level-2 environmental products from geo-Iocated and calibrated level 1.5 COMS data. Preliminary design for the level-2 data processing system consists of 16 baseline products and will be refined by end of 3rd project year. Also considered for the development are the necessary initial information such as land use and digital elevation map, algorithms for the vicarious calibration and procedures for the calibration monitoring, and radiative transfer model. Here, we briefly introduce the overall development strategy, flow chart for the intended baseline products, a few preliminary algorithm results and future plans.

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Technology Opportunity Discovery Based on Firms' Technologies and Products (기업의 보유 기술 및 제품에 기반한 기술기회발굴)

  • Park, Hyunseok;Seo, Wonchul;Coh, Byoung-Youl;Lee, Jae-Min;Yoon, Janghyeok
    • Journal of Korean Institute of Industrial Engineers
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    • v.40 no.5
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    • pp.442-450
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    • 2014
  • Technology opportunity discovery (TOD) based on technological capability is a process which identifies new product and technology items that can be developed by utilizing or improving a firm's existing products or technologies. By taking into consideration the investment risk of R&D and its practicality, developing technological capability-based TOD methodology is considered to be important for both business and research. To this end, we propose a technological capability-based TOD method and its system using TOD knowledge base. The method can support four types of TOD cases, which are based on a firm's existing technologies and products, and TOD knowledge base is developed by using function information extracted from patent documents. In this paper, we introduce the overall framework of the method and provide application examples on the four TOD cases using the prototype system.

A Study on the Remanufacturing of Used Machine Tools (노후된 공작기계의 재제조에 관한 연구)

  • Roh, Young-Hwa
    • Journal of the Korean Society of Industry Convergence
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    • v.23 no.3
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    • pp.403-410
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    • 2020
  • Continuous industrial development has led to a better quality of life for everyone, even further accelerating industrial growth. Industrial development, however, has also caused environmental degradation, which is posing a serious threat to humanity. It has also encouraged the indiscriminate use of limited resources, causing resource depletion. Efficient resource management based on resource circulation is critical to saving resources. Resource circulation methods are as follows: reducing the use of resources in the manufacturing process, recycling used or reprocessed products and reusing used resources without being reprocessed, remanufacturing with end-of-life products with disassembled parts. Furthermore, remanufacturing process including cleaning, inspection, repairing, and reassembling facilitate performance level as well as new typical products. It is noteworthy that the remanufacturing of machine tools can significantly save resources because their structural parts are substantially large in size. Machine tools have served as a foundation for the manufacturing industry, which has driven Korea's industrial development. Nevertheless, a few research has been reported for remanufacturing technology with used machine tools. Relevant research of developing a remanufacturing process chart and method is prerequisite for saving the resource and environments.

Evaluation Method of Hairstyling Materials and its Application to Cosmetic Preparations

  • Abe, Hidetoshi;Iida, Ichiro;Someya, Takao
    • Proceedings of the SCSK Conference
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    • 2003.09a
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    • pp.1-12
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    • 2003
  • Instead of sensory evaluation, we designed an evaluation method of the setting function of hairstyling products, based on an original theory focusing on changes in bending stress observed when a load with continuous bending is applied to human hair. Specifically, we developed a device to measure bending stress to quickly and objectively evaluate the condition of human hair, particularly its dynamic properties such as the setting function, following the application of hairstyling products. This device generates a load with continuous bending while applying a pendulum motion to a hair tress, one end of which is anchored. The setting function and holding power of resins of various molecular weight and ionic properties were evaluated using this device. The results demonstrated a close correlation with those obtained by experts' sensory evaluation. The evaluation results of bending stress and holding rate confirmed that the combined use of two different resins could improve the function of setting preparations. Evaluation using this device was able to substitute for sensory evaluation, and offers quick objective evaluation and detection of changes in the holding power of hairstyling products over time. We conclude that evaluation using this device is a promising new method.

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Intelligent Control Algorithm for the Adjustment Process During Electronics Production (전자제품생산의 조정고정을 위한 지능형 제어알고리즘)

  • 장석호;구영모;고택범;우광방
    • Journal of Institute of Control, Robotics and Systems
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    • v.4 no.4
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    • pp.448-457
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    • 1998
  • A neural network based control algorithm with fuzzy compensation is proposed for the automated adjustment in the production of electronic end-products. The process of adjustment is to tune the variable devices in order to examine the specified performances of the products ready prior to packing. Camcorder is considered as a target product. The required test and adjustment system is developed. The adjustment system consists of a NNC(neural network controller), a sub-NNC, and an auxiliary algorithm utilizing the fuzzy logic. The neural network is trained by means of errors between the outputs of the real system and the network, as well as on the errors between the changing rate of the outputs. Control algorithm is derived to speed up the learning dynamics and to avoid the local minima at higher energy level, and is able to converge to the global minimum at lower energy level. Many unexpected problems in the application of the real system are resolved by the auxiliary algorithms. As the adjustments of multiple items are related to each other, but the significant effect of performance by any specific item is not observed. The experimental result shows that the proposed method performs very effectively and are advantageous in simple architecture, extracting easily the training data without expertise, adapting to the unstable system that the input-output properties of each products are slightly different, with a wide application to other similar adjustment processes.

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Properties and Curing Behavior of Urea-Modified Phenolic Resins

  • Lee, Sang-Min;Park, Sang-Bum;Park, Jong-Young
    • Journal of the Korean Applied Science and Technology
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    • v.24 no.4
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    • pp.339-346
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    • 2007
  • The influence of adding urea to phenol-formaldehyde (PF) resins as a co-polymer component were investigated aiming at synthesizing useful phenol-urea-formaldehyde resins. Urea was added at 10% by total resin weight. Several methods for the addition of urea to the PF resins during synthesizing resins to see the co-polymerization occurs between urea and PF resins. The urea was added at the beginning, at three different middle stages, and at the end of PF resin synthesis. The copolymerized methylene bridges between phenol and urea molecules were not observed by 13CNMR; no signal around 50ppm. The curing of urea-modified PF resins, evaluated by dynamic mechanical analysis(DMA), showed some differences among the resins. DMA gel times ranged from 2.75 min to 3.25 min and the resins made with earlier urea additions showed slightly shorter gel times. The longest cure time and gelation time was observed for the resin PFU. Catalyst effects on the DMA cure time values of resins were not significant with different amounts of catalyst or different types of catalyst for all resins tested. Gel times of urea-modified PF resins shortened the most by triacetin catalyst.

An Input-Output Analysis on the Economic Effect of the Korean First Medium-term Logistics Plan (2001-2005)

  • Pak, Myong-Sop;Yoon, Jae-Ho
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.40
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    • pp.33-63
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    • 2008
  • This study introduces the adoption of the logistics industry by the Korean government as a threshold of overcoming economic difficulties. The core contents of the National Logistics Plans (2001-2020) which contain the basic principles of the logistics policies of the Korean government and its aim to be an 'Excellent Nation of Logistics' is introduced. The economic effect of the logistics policies implemented between 2001 and 2005, (the period of the first Medium-term Logistics Plan according to the National Logistics Plans (2001-2020) set up by the Korean government at the end of 2000) is estimated through input-output analysis. The input-output analysis result is as follows: the total output effect is 8,856 billion won of which indirect output effect is 3,982.9 billion won; indirect output effect comes mainly from real estate and business services, non-metal products, metal products, electrical and electronics products, finance and insurance, wholesale and retail, petroleum and coal; the total amount of value-added effect is 3,376 billion won and total import effect is 726 billion won. Employment effect including self-employed and unpaid family supporters with paid laborers is 79,203.7 people of which paid laborers comprise 67,547.7 people.

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