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Selection of Early and Late Flowering Robinia pseudoacacia from Domesticated and Introduced Cultivars in Korea and Prediction of Flowering Period by Accumulated Temperature

  • Lee, Kyung Joon;Sohn, Jae Hyung;Redei, K.;Yun, Hye Young
    • Journal of Korean Society of Forest Science
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    • v.96 no.2
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    • pp.170-177
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    • 2007
  • The objectives of this study were to select early, late, and abundant flowering trees of black locust from domesticated and introduced cultivars, and prediction of flowering period by calculation of accumulated temperature in spring. Four cultivars (Debreceni-2, Pusztavacs, Jaszkiseri, and Rozsaszin AC) from Hungary and a cultivar from Beijing, China, were introduced, propagated by seed and planted in a seed orchard. For domesticated black locust, 63 cultivars from 10 locations throughout the country were selected and propagated by root cutting. Criteria for selection of domesticated cultivars were abundant flowering, long flowering period, or abundant nectar production with, if possible, straight stems. Accumulated temperature was calculated from data of a nearby weather station by accumulating daily maximum temperature minus 5 degree Celsius from January 1 up to the date reaching 880 degrees. Daily mean temperature was also used to calculate accumulated temperature up to the date reaching 450 degrees. The percentages of two-year and three-year-old flowering trees propagated by root cutting were higher than that of trees propagated by seeds, while four-year-old trees all flowered regardless of propagation methods. Among the domesticated cultivars, all the cultivars from Ganghwa showed abundant flowering with highest nectar production of 6.5 ul per flower, which was 100% more than other domesticated cultivars and 50% more than Debreceni-2 cultivar with highest nectar production among the introduced cultivars from Hungary. At the end of the eight years of observations, two trees of Debreceni-2 cultivars and a tree from Beijing, China were selected for early flowering trees which flowered 2 to 3 days earlier than average trees, while a tree of Debeceni-2 and three trees from Bejing were selected for late flowering trees which flowered 2 to 3 days later than average trees. It is possible to extend the flowering period of black locust by 4 to 6 days by planting early and late flowering cultivars together. Abundant flowering trees were unable to be selected due to severe damages by leaf gall midges which killed many trees and reduced the crown size of the remaining trees in the seed orchard, and which were first found in Korea in 2001 and now damaging most of the black locust forests in Korea. The prediction of flowering period by accumulated temperature indicated that black locust flowered to a peak when accumulated daily maximum temperature reached 880 degrees Celsius, and when daily mean temperature reached 450 degrees.

Exploration of Functional Materials from Oriental Medicine Extracts Cultured with Tricholoma Matsutake Mycelium - (2) Effect of Extracts on Blood Glucose and Liver Function in Streptozotocin-Induced Diabetic Rat -

  • Kim, Hae-Ja;Choi, Yun-Hee;Cho, Hwa-Eun;Hong, Hak-Gi;Han, Jung-Ho;Lee, Ki-Nam
    • Journal of Society of Preventive Korean Medicine
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    • v.12 no.2
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    • pp.13-25
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    • 2008
  • The purpose of this study was to investigate extract from mixed culture with Trichloloma matsutake mycelium in oriental medicine and cereal medium(OCM) to develop new material for pharmaceutical products and medicinal food for diabetes mellitus. To evaluate of hypoglycemic activity of OCM extracts, we examined the inhibitory activity of ${\alpha}$-glucosidasein OCM, blood glucose level and liver function of streptozotocin(STZ) induced diabetic rat. Experimental group was divided into 6 groups: first, it was divided into normal control group(hereafter NC group) and diabetes-induced group, and diabetes-induced group was subdivided into diabetic control group(DC group), treated by hot water extracts group(HE), ultra sonic waves, micro waves, and micro bubble extracts g roup(UE), crude polysaccharide of HE group (HEE) and crude polysaccharide of UE group(UEE) at a dose of 300mg/kg/body weight, respectively. In diabetic-induced groups, after streptozotocin was melted in 0.01M citrate buffer at 50mg/kg/body weight, when the non-fasting blood glucose levelwas 300 mg/dl or more in blood collected from the tail vein, it was regarded as diabetic induction and then such diabetic-induced experimental animals were used in this experiment. At the end of the experiment, blood glucose level increased by 4.19% in DC group but significantly decreased by 32.34%, 19.19%, 17.81% and 17.64%, respectively in UEEE, UE, HE, and HEE groups. In the cases of AST, ALT, and ALP, the experiment group treated with extracts showed significantly lowerblood glucose level than DC group. The levels of BUN and uric acid were found to be lower in the UMPM extract group(UE) than HW extract group(HE), which implies that herb medicine medium extracts in which Tricholoma matsutake mycelia were cultured are effective in reducing impaired liver function as well as high blood glucose level caused by diabetes. In addition, the administration of low temperature UMPM extracts was found to produce better results than that of high temperature hot water extracts. In this regard, it is expected that extracts from herb medicine obtained by cultivating Tricholoma matsutake mycelia will be widely used as new ingredients for foods and medicines for prevention and treatment of diabetes.

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A Study on the Probability of BLEVE of Above-ground LP Gas Storage Tanks Exposed to External Fire (지상식 LPG 저장탱크의 외부화재에 의한 BLEVE 가능성 해석)

  • Lee Seung-Lim;Lee Young-Soon
    • Journal of the Korean Institute of Gas
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    • v.7 no.1 s.18
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    • pp.19-23
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    • 2003
  • The purpose of this thesis is to investigate the BLEVE probability of LP gas storage tanks which are relatively more dangerous, by the deductive calculating method using the results of Birk's pilot tank test and the required heat capacity of BLEVE. The result that BLEVEs can occur in only above 43.68 percent of liquid filling level under $600^{\circ}C$ of tank pate temperature and $53^{\circ}C$ of inner liquid temperature, was obtained and will be useful for preventing the BLEVE of LP gas storage tanks in fire sites. In addition, this research showed conditions of external leak and fire causing BLEVE, based on 15ton capacity of LP gas tank which has the same specifications as those in Puchon LP gas filling station accident. The result of the calculation is that the minimum pool fire conditions of BLEVE are above 7.2mm equivalent diameter under a liquid release condition and above 17.6mm equivalent diameter under a two-phase release condition. In the end, the result of calculating the pool size corresponding the above conditions using EFFECTS version 2.1, concludes that a minimum of 3.3 meters of diameter and 10.4 meters of height should be needed for BLEVE outbreak.

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Some Observations on the Organelles Participating in the Biliary Excretion in the Hepatocyte of the Biligrafin Injected Mouse (Biligrafin 투여 마우스 간세포의 미세구조적 및 세포화학적 연구)

  • Kim, Hyang;Shin, Young-Chul
    • Applied Microscopy
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    • v.23 no.2
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    • pp.53-77
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    • 1993
  • In this study, an attempt was made to investigate the probable organelles participating in the secretion of biligrafin. The animals (ICR male mice, 25-30gm) were divided into normal control and 6 biligrafin injected groups to which 30% biligrafin (0.006ml/gm b.w.) were injected at 10, 20, 40, 80, 160 and 320 min prior to the sampling. The mice of each group were perfused through the heart with ice-cold 2.5% glutaraldehyde buffered with 0.1M Na-cacodylate (pH. 7.4) under the Na-pentobarbital (Nembtal 0.0015mg/gm b.w.) anesthesia and liver tissues were taken from each group. Some specimens were immersed 1 hr in the same solution used in the perfusion. After an overnight rinse in 0.1M Na-cacodylate buffer containing 10% DMSO and 7.6% sucrose, $75{\mu}m$ fronzen sections were made for cytochemical study. The sections were incubated in thiamin pyrophosphatase (TPPase) and inosine diphosphatase (ID Pase) media for 70 min at $37^{\circ}C$ respectively and acid phosphatase (AcPase) medium for 40 min at $37^{\circ}C$. They were postfixed in 1 % $OsO_4$ for 1 hr. The other specimens were immersed for 8 hrs in the fixative consisting of 2.5% glutaraldehyde and 3.0% paraformaldehyde buffered with Na-cacodylate (pH. 7.4). All of the osmificated specimens were processed for electron microscopy. In both normal and biligrafin injected groups, endoplasmic reticulum (ER), vacuoles, Golgi apparatus and lysosomes were seen in the vicinity of bile canaliculus. In the biligrafin injected groups, however, the Golgi apparatus appeared to be decreased and ER and vacuoles were dilated and increased. The rough endoplasmic reticulum (RER) having a few attached ribosomes appeared to be the round saccule, especially at 20 min after biligrafin injection. Smooth endoplasmic reticulum (SER) seemed to be formed by the detachment of ribosomes at the cisternal end of RER. The cistern of SER showed saccules which probably budded off to form the vacuole. The vacuoles were devoid of visible centents. This finding seemed to be in agreement with the biochemical property of the bile constituents. The fusion between the vacuoles and bile canaliculus were frequently seen in the groups injected with biligrafin. The lysosome did not show any changes in the biligrafin injected groups. Accumulation of some material and lipid droplets were seen at the 40 and 80 min after biligrafin injection, especially at the latter. At 160 and 320 min after biligrafin injections, however, they were decreased successively while the RER stack, free ribosomes and polysomes were increased. Although the reactive products of TPPase and IDPase were observed in the ER saccules and vesicles of the normal control and biligrafin injected groups, the fusion between the bile canaliculus and saccules or vesicles could easily be seen in the latter. The AcPase activity, however, was observed in the cistern at the maturing face of Golgi apparatus and lysosomes in both normal and biligrafin groups. The results suggest that the biligrafin is excreted via the vesicles, vacuoles or sacoules probably derived from the SER without the participation of Golgi apparatus and lysosomes, and the excess amount of material is stored as inclusions during the repairing of the organelles being overactive.

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Effects of processing method and storage temperature and time on the texture of Yaksik(cooked and seasoned glutinous rice) (약식의 제조방법과 저장온도 및 기간에 따른 조직감의 변화)

  • Lee, Hei-Jeung;Lee, Young-Keun;Koo, Sung-Ja;Hong, Sung-Hee;Lee, Cherl-Ho
    • Journal of the Korean Society of Food Culture
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    • v.3 no.4
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    • pp.391-396
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    • 1988
  • The method for the measurement of texture hardening phenomena, which is the limiting factor of shelf-life of Yaksik in the market, was established. The changes in the hardening rate by the processing conditions and the storage temperature and time were examined. The standard sample made by traditional method could be kept at room temperature$(20{\circ}C)$ for 3 days and the multipuncture force measured at the end of marketable quality was 700g. The hardening rate increased rapidly by storing at $5^{\circ}C$ and the ratio of hardening rate constants between room temperature and $5^{\circ}C$ storage reached to $1.3{\sim}3.3$ depending on the processing condition. The largest ratio was observed by the sample made from pressure cooker. The addition of corn syrup retarded the hardening rate. The pressure cooking resulted in making too soft product, which diminished the panel preference, but it extend the shelf-life when products were stored at $5^{\circ}C$ microwave cooking resulted in making too hard texture which was not acceptable. The overall quality preference of Yaksik was decided by the textural preference and the latter showed significant inverse correlation with the maximum force of multipuncture test. Therefore, it was concluded that multipuncture test was useful for the measurement of the quality of Yaksik.

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Production of Xylooligo-Saccharides and Purification of Extracellular Xylanase from Streptomyces chibaensis J-59 (방선균 Streptomyces chibaensis J-59 Xylanase의 정제 및 자일로 올리고당(Xylooligo-Saccharides)의 생산)

  • Joo, Gil-Jae;Rhee, In-Koo
    • Current Research on Agriculture and Life Sciences
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    • v.14
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    • pp.111-122
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    • 1996
  • S. chibaensis J-59 produced an extracellular xylanase in a CSL medium composed of 1.5% com steep liquor, 0.1% $MgSO_4{\cdot}7H_2O$, 0.012% $CoCl_2{\cdot}6H_2O$, and 0.15% glucose containing xylan. but it did not produce in the culture medium containing xylose. The production of enzyme reached to a maximum level (0.83 uints/ml) when bacteria were cultured in 2.5 l jar fermentor for 48hrs at $30^{\circ}C$ and pH 7.0. Furthermore, S. chibaensis J-59 produced an intracellular glucose isomerase in a medium containing xylan and/or xylose. Xylanase was purified 29-fold over the culture supernatants of S. chibaensis J-59 by ammonium sulfate fractionation, chromatography on DEAE-Sephadex A-50, and gel filtration on Sephadex G-200. The purified enzyme is a monomeric enzyme with a native molecular mass of 25 kDa and a subunit molecular mass of 25 kDa. The purified enzyme requires $Mg^{2+}$ for activity, $Ca^{2+}$, $Co^{2+}$ is not an inhibitor but inhibit by $Fe^{3+}$, $Hg^{2+}$, and $Cu^{2+}$, sodium dodecyl sulfate, N-bromosuccinide. Pattern of hydrolysis demonstrated that the xylanase was an endo-splitting enzyme able to break down birchwood xylan at random giving xylobiose, xylotriose and xylotetrose as the main end products.

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Production Conditions of Xylanase from Streptomyces thermocyaneoviolaceus and Production of Xylooligosaccharides (Streptomyces thermocyaneoviolaceus의 Xylanase 생산조건 및 Xylooligo당의 생산)

  • Choi, Jun-Ho;Kwon, Dal-Ho;Lee, Oh-Seuk;Joo, Gil-Jae;Park, Heui-Dong;Rhee, In-Koo
    • Current Research on Agriculture and Life Sciences
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    • v.16
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    • pp.45-54
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    • 1998
  • A thermotolerant bacterium, Streptomyces thermocyaneoviolaceus which produced xylan-degrading enzymes, utilized excellently xylan of wheat bran by producing the enzymes in comparison with that of birchwood or oat spelts. Optimal enzyme production was achieved in WB medium containing 0.8% wheat bran, 0.06% yeast extract, 0.06% bactopeptone, 0.05% $MgSO_4{\cdot}7H_2O$, 0.05% $FeSO_4{\cdot}7H_2O$, 0.05% $KH_2PO_4$ and, 0.2% $K_2HPO_4$(pH 7.0) at $50^{\circ}C$ for 24 hrs. The optimal pH and temperature for the hydrolysis of xylan were pH 5.5 and $65^{\circ}C$, respectively. The enzyme activity was retained more than 80% at the range from pH 4.5 to pH 9.5 at $4^{\circ}C$ for 12 hrs and 94% on the heat-treatment at $65^{\circ}C$ for 1 hr. Xylobiose, xylotriose, xylose, and other xylooligosaccharides were produced as end products from hydrolysis of birchwood xylan by the xylanase of S. thermocyaneoviolceus.

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Visual Image of a Yangbanxi (Chinese "Model Play") Dating from the Cultural Revolution Period in China - With the Focus on Images of Revolutionary Heroes in the Beijing Opera The Red Lantern (중국 문혁기 모범극(樣板戱)의 시각이미지 - <홍등기(紅燈記)>의 혁명영웅상을 중심으로)

  • Moon, Jung-Hee
    • The Journal of Art Theory & Practice
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    • no.5
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    • pp.197-215
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    • 2007
  • Yangbanxi("model plays") symbolize the Cultural Revolution(1966~1976) in China. The Eight Model Revolutionary Works include five Chinese Modern Peking Operas, namely, Taking Tiger Mountain by Strategy(智取威虎山), Harbor(海港), Shaijabang(沙家浜), The Red Lantern(紅燈記), and Raid on the White Tiger Regiment ; (奇襲白虎團), ballets such as The Red Detachment of Women(紅色娘子軍) and The White-Haired Girl(白毛女) ; and a symphony: Shajiabang(沙家浜). On April 24, 1967, Chinese leaders, including Mao Zedong, saw a performance of The White-Haired Girl. Yanbanxi was performed in Beijing between May 24 and June 15 the same year. The Red Lantern was designated as a work for the proletarian classes by Jiang Qing(Mao Zedong's third wife and the most influential woman in China) and spread nationwide. It was also made into a film to be enjoyed by many people. The modern Chinese operas went a long way in their creation of visual images of revolutionary heroes. The Red Lantern, in particular, came to be regarded as the most representative revolutionary opera. In the course of such a process, Jiang Qing used Yangbanxi as a political tool for compelling the people to worship and pledge their allegiance to Mao in an effort to turn the Cultural Revolution into a class struggle on behalf of her husband. During the Cultural Revolution period, artists were made to associate with workers, farmers and soldiers based on the idea of advocating revolutionary arts for the proletarian classes. The characters in The Rend Lantern were portrayed as heroes from the proletarian classes according to the demands of the era. Chinese leaders set forth the principles of artistic expression, stressing three important factors: politics, heroes, and heroic acts, which were to be applied to all the visual arts, including Yangbanxi. This paper attempts to present a new view of fine arts during the Cultural Revolution in China by focusing on the productive significance of a leading style of a specific era in the past. To that end, this paper sheds light on products made in conformance with political instructions, stressing the importance of revolutionary heroes in The Red Lantern.

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Synthesis of Poly(oxyethylene-co-adipate)-diol from Adipic Acid and Polyethylene Glycols: Effect of Catalyst Concentration (아디프산과 폴리에틸렌글리콜로부터 폴리(옥시에텔렌-아디페이트)-디올 공중합체 합성: 촉매농도의 영향)

  • Jung, Yong-Sung;Lee, Sang-Ho
    • Elastomers and Composites
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    • v.49 no.2
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    • pp.110-116
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    • 2014
  • We investigated the effect of the concentration of stannous 2-ethylhexanoate catalyst on the esterification rate between adipic acid (AA) and each of two PEG oligomers, diethylene glycol (DEG) and polyethylene glycol (PEG600). The concentration of the catalyst was varied from 0.15 to 2.0 wt.%. To attach hydroxy group to each end of the poly(oxyethylene-co-adipate) synthesized from AA and the PEGs, the esterification was performed with excessive PEG oligomers ([PEG]/[AA]=2) at $170^{\circ}C$. The degree of polymerization of the poly(oxyethylene-co-adipate)diol products were three. The apparent rate constant ($k_{app}$) of the esterification between AA and DEG shows the first order dependency on the catalyst concentration ($k_{app}=0.88[C_{cat}]$), whereas the $k_{app}$ of the esterification between AA and PEG600 has a relation of $k_{app}=0.123[C_{cat}]^{0.55}$ with the catalyst concentration. It is expected that the rate of esterification between AA and DEG has a non-linear dependency on the catalyst concentration as the catalyst concentration approaches to 0.22M.

A Study on the Education of the Fisheries School of Korea in Japanese Colony (일제하의 수산학교 교육에 관한 연구)

  • Shin, Qui-Won;Kim, Sam-Kon;Chi, Ho-Weon;Kim, Jae-Sik;Kim, Tae-Wun
    • Journal of Fisheries and Marine Sciences Education
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    • v.11 no.1
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    • pp.69-87
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    • 1999
  • This study had been analysed the establishment, the closing and the actual conditions of the fisheries school according to the four times revision and promulgation of the law of education of korea which were devided by the early term (the first Chosun educational decree), the middle term (the second Chosun educational decree) and the last term (the third and fourth Chosun educational decree), and also been investigated how the fisheries education of the school had been acted to the mobocracy and the assimilation policy and together with it's back ground through this analysis. The aim of this study is to contribute to the study of the history of the fisheries school education, analysis of change of the fisheries school education according to practical application of the Chosun educational decree. The summary of the characteristic of the each term are as under. First, in the early term of the fisheries education under the first Chosun educational decree, Experts were despatched to the each province with donated money from emperor and opened seasonal the fishing training centre, a kind of social fisheries education, and trained directly fishing technic and were going to train fisheries trainees regularly under name of elementary fisheries school. Japanese imperialism attached great importance to the vocational education in order to snack economical products from colonized Korea but actually had a purpose to train low quality technician who follow blindly their colony policy of Japanese imperialism. The fisheries schools in the circle of system in early time of Japanese imperialism, Kunsan public elementary fisheries school was established in April 1915, Yosu public elementary fisheries school was established ill May 1917 and Kyungnam Tongyoung training school was established in March 1917. Secondly, After 3.1 movement, the Japanese imperialism established an appeasement policy so called cultural politics and continued assimilation policy with skilful methods. After revision of the second Chosun educational decree, the Elementary vocational school was changed as the vocational training school. The school of fisheries education in middle of Japanese imperialism trained low quality technicians to snack fisheries resources from colonized Korea. After the middle of Japanese imperialism they paid attention on training fisheries technician through fisheries school rather than training school. With high interest and crowded volunteers, Kunsan public fisheries school was promoted in 1922, Tongyoung public fisheries school was promoted in 1923, Yongampo fisheries training school established in 1922 was promoted as Yongampo public fisheries school in 1926. Thirdly, in the time of the third and fourth Chosun educational decree, the end of Japanese imperialism, they met Pacific war after Japan vs China war. During the war time they considered the vocational school as the source of supply for materials and manpower and consequently had to expanded vocational education and systematically despatched students to war field and practiced military training. In 1938, Namhae public fisheries school was established and Chungjin fisheries school was permitted. But in order to supply manpower to Pacific war, the study period of Yosu public fisheries school was shorten from 5 years to 4 year in 1943 and also that of Tongyoung public fisheries school shorten in 1944.

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