• Asian-Australasian Journal of Animal Sciences
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• 제21권6호
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• pp.831-837
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• 2008

The objective of this study was to evaluate the effects of ruminally-protected methionine supplementation (0, 7, or 14 g/head/d) on nutrient intake, digestibility, growth performance, carcass, and meat characteristics of Awassi ram lambs fed finishing diets. Twenty four Awassi ram lambs ($16.8{\pm}1.17kg$ body weight) were randomly assigned to 3 treatment diets (8 lambs/treatment) and housed in individual pens. Lambs were given an adaptation period of 7 days before the intensive feeding period that lasted for 86 days. On day 74 of the trial, a digestibility experiment was performed. At the end of the trial (d 86), all lambs were slaughtered to evaluate carcass characteristics and meat quality. Increasing the level of methionine supplementation did not improve (p>0.05) performance nor feed conversion ratio. Nutrient intake and digestibilities were not influenced (p>0.05) by methionine supplementation. There were no differences in final weight, hot and cold carcass weights, dressing percentages or any of the measured non-carcass components. Tissues and fat depth measurements together with all meat quality attributes measured on longissimus muscle of the loin cut were not affected by methionine supplementation. The only meat quality parameters affected were redness (a*) and the hue angle being higher for the control group (p<0.05). These results suggest that methionine supplementation is not likely to produce any production benefits in nutrient digestibilities, performance or carcass characteristics of ram lambs fed a high performance diet.

• Asian-Australasian Journal of Animal Sciences
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• 제25권6호
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• pp.832-838
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• 2012

This study was conducted to evaluate the effects of dietary supplementation of catechins and wheat bran on growth performance, carcass characteristics and meat color using forty-eight Hanwoo steers. Each steer was randomly assigned to one of four treatments. Treatments were as follows: control, catechins, wheat bran, and catechins+wheat bran (CW). At the end of the feeding trial, blood samples were collected for analysis of hemoglobin and iron concentration, and then steers were slaughtered at a commercial abattoir. Catechins, wheat bran, and CW had no effect on growth performance and carcass characteristics. Percentage of myoglobin in M. longissimus dorsi was not affected by treatments. Percentage of oxymyoglobin was increased (p<0.05) by CW and maintained at the highest level (p<0.05) for CW during meat display time up to 24 h. Percentage of metmyoglobin was the lowest (p<0.05) for CW between 5 and 24 h during display time. Although blood iron concentration was not affected by treatments, total concentration of muscle iron was the lowest (p<0.05) for CW compared with that of other treatments. Therefore, the results of the present study suggest that CW may provide good protection against oxidation of myoglobin, and might be an effective dietary supplement for improving meat color in the late fattening period of Hanwoo steers.

• Asian-Australasian Journal of Animal Sciences
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• 제33권12호
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• pp.1940-1947
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• 2020

Objective: Combination of two stressors on alteration of mineral footprints in animals needs due attention to meet maximum production and welfare, particularly in grazing sheep. This study tested whether ewes (Ovis aries) exposed to water deprivation and thermal-humidity stressors had altered mineral footprints in their wool, serum, urine, and feces. Methods: Nine ewes (age = 3 years; mean body weight = 41±3.5 kg) were divided among a control group with free access to water, and treatment groups with water deprivation lasting either 2 h (2hWD) or 3 h (3hWD) after feeding. Using a 3×3 Latin square design, animals were assigned to treatment groups for three sampling periods of 21 days each (n = 9). Blood was collected by jugular venipuncture. Wool was collected at the end of periods 2 and 3. Metabolic crates designed with metal grated floors were used for urine and feces collection. We measured sodium (Na), magnesium (Mg), phosphorus (P), chloride (Cl), calcium (Ca), manganese (Mn), copper (Cu), iron (Fe), and zinc (Zn). Results: The wool mineral levels did not differ between the treatment groups, although K was marginally lower (p = 0.10) in the 2hWD group. The serum and urine mineral levels did not differ between the treatments (p>0.05). Fecal K was significantly lower in the 2hWD group than in the other groups (p≤0.05). Conclusion: In conclusion, water deprivation and thermal-humidity exposure altered the excretion of K, but not of other minerals, in the wool, urine, feces, or serum of ewes. Thus, no additional mineral supplementation is needed for water deprived ewes during thermalhumidity exposure.

• Fisheries and Aquatic Sciences
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• 제18권4호
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• pp.367-372
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• 2015

We investigated the effects of dietary supplementation of two types (powder or liquid) of propolis on innate immunity and disease resistance of olive flounder Paralichthys olivaceus against Edwerdsiella tarda. A total of 600 fish averaging 30 g were randomly distributed into 24 tanks in groups of 25. Three tanks were assigned to each of eight experimental diets: 0 (Control), 0.25, 0.5, 0.75 and 1 % propolis in powder form and 0.25, 0.5 and 1 % propolis in liquid form (PP0.25, PP0.5, PP0.75, PP1, LP0.25, LP0.5 and LP1, respectively). Fish were fed each experimental diet twice daily for four weeks to apparent satiation. At the end of the feeding trial, lysozyme and myeloperoxidase activities and total immunoglobulin level were significantly higher in fish fed the PP1 and LP0.5 diets compared to those fed the control diet. The PP1 diet was also associated with a significant increase in anti-protease activity compared to the control diet. After challenge with E. tarda, fish fed the LP0.5 diet showed numerically higher survival compared to the other groups. This study indicates that non-specific immune responses of olive flounder can be enhanced by dietary supplementation with powder and liquid forms of propolis, and that the optimal level would be 1% in powder form or 0.5% in liquid form. It seemed that growth performance and feed utilization are not affected by the propolis supplementation in diets for olive flounder.

• Fisheries and Aquatic Sciences
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• 제17권4호
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• pp.433-439
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• 2014

We compared the utilization efficiency of free lysine (FL) and dipeptide lysine-glycine (LG) in terms of growth performance and whole-body amino acid composition in olive flounder Paralichthys olivaceus. A basal experimental diet was formulated to contain 0.5% (basal) lysine from fish meal, and four other diets were prepared by supplementing 0.5% or 1.0% of either FL or LG. The experiment was performed in triplicate, and 20 randomly selected fish averaging $5.41{\pm}0.16g$ were fed one of the test diets at the rate of 3% BW/day twice daily for 6 weeks. At the end of the feeding trial, fish fed the basal diet showed significantly less weight gain than did the other groups. The results of a two-way ANOVA showed that both lysine level (P = 0.001) and type (P = 0.034) influenced growth rate; however, we found no significant interaction between lysine level and form (P > 0.05). Our results revealed a significant improvement in protein efficiency ratio (PER) with each increment of dietary lysine, and the groups fed LG-supplemented diets showed higher PER than did those offered FL. Hepatosomatic and viscerosomatic indices were significantly influenced by lysine level and form, and higher values were recorded in fish fed diets containing LG. Significantly higher whole-body arginine levels were found in LG-fed groups, and a significant interaction was observed between lysine level and form (P = 0.009). Whole-body valine and aspartic acid contents were affected by lysine level, and alanine concentration was influenced by both lysine level and form. Our findings indicate that juvenile olive flounder can utilize LG more efficiently than FL for protein synthesis.

• Fisheries and Aquatic Sciences
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• 제16권1호
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• pp.57-61
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• 2013

I hypothesized that the manipulation of dietary nutrient contents including phosphorous could affect compensatory growth of juvenile olive flounder, Paralichthys olivaceus. Thirty fish averaging 34.8 g per tank were randomly chosen and distributed into 15 flow-through 180-L tanks. Three experimental diets were prepared: the control (C) diet, high protein and lipid (HPL) diet, and HPL diet with supplementation of calcium phosphate-monobasic (HPLP). Five treatments were prepared in triplicate: fish were hand-fed daily with the C diet twice a day for 8 weeks (C-8W); fish were starved for 1 week, and then fed with the HPL or HPLP diets twice a day for 7 weeks, and referred to as HPL-7W and HPLP-7W, respectively; and fish were starved for 2 weeks, and then fed with the HPL or HPLP diets twice a day for 6 weeks, and referred to as HPL-6W and HPLP-6W, respectively. The body weight of fish with C-8W, HPL-7W and HPLP-7W treatments was higher than fish with HPL-6W and HPLP-6W treatments on week 2, 4 and 6 after an initiation of the trial. At the end of the 8-week trial, fish with HPLP-7W and HPL-7W treatments overcompensated, as compared to fish with C-8W treatment. Full compensation was not achieved in fish subjected to the 2-week feed deprivation (HPL-6W and HPLP-6W treatments). Overall feed intake by fish was proportional to weeks of feeding. Feed conversion ratio of fish with HPLP-7W, HPL-6W and HPLP-6W treatments was higher than fish with C-8W treatment. The study showed that dietary supplementation of protein and lipid resulted in overcompensation of juvenile olive flounder subjected to a 1-week feed deprivation, but not a 2-week feed deprivation. Additionally, dietary supplementation of phosphorous did not further improve compensatory growth of fish.

• 한국수산과학회지
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• 제50권6호
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• pp.762-769
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• 2017

This study investigated the effects of stocking density on the growth of the mandarin fish Siniperca scherzeri, in small (1-year old, 50.2 g/fish) and large (2-years old, 173.1 g/fish) size (age) groups. Small and large fish groups were fed pelleted diets twice daily for 10 and 15 weeks, respectively. In the small group, at the end of the feeding, the weight gain of fish at a density of $4kg/m^3$ was lower than that of fish at densities of 3, 2 and $1kg/m^3$. However, fish stocking density did not affect feed efficiency. The daily feed intake of fish at a density of $4kg/m^3$ was lower than that fish at densities of 3, 2 and $1kg/m^3$. In the large fish group, the weight gain of fish at a density of $4.5kg/m^3$ was higher than that of fish at densities of 6, 3 and $1.5kg/m^3$. Again, fish stocking density did not affect feed efficiency. The daily feed intake of fish at a density of $4.5kg/m^3$ was higher than that of fish at densities of 6, 3 and $1.5kg/m^3$. Based on these results, the optimum stocking density for small (50.2 g/fish) and large (173.1 g/fish) mandarin fish is 3 and $4.5kg/m^3$, respectively.

• Ocean and Polar Research
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• 제36권2호
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• pp.145-156
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• 2014

Macrobenthic biodiversity in the rocky intertidal areas of the Tae-an region, Republic of Korea, has decreased since the Hebei Spirit oil spill in December 2007. We aimed to investigate ecological roles of Pacific oyster (Crassostrea gigas) because recruitment and growth of oysters are critical to the recovery of damaged rocky shore ecosystem. We surveyed two sites monthly: natural rocky substrate and farming substrate, from July 2012 to January 2013 to identify and compare the changes in macrobenthic fauna. The abundance of young oysters was higher at the natural site. On the other hand, the mean height of oyster on the farming substrate was more than twice as great. The abundance of oyster at the natural site increased until October and then continuously decreased until end of study period. However, the abundance of oyster at the farming site constantly decreased from the beginning of study period. These different growth patterns might be attributable to spatial competition between oyster and a barnacle species (Balanus albicostatus) and environmental factors. At the natural site, physical stress factors including dramatic temperature changes and desiccation a few of the major factors limiting growth during aerial exposure. In addition, motile macrobenthos could be detrimental to oysters because they interrupt filter-feeding activities and hence hamper the growth of oysters. We show the higher recruitment of oysters at the natural site and healthy growth in the farming substrate are due to complicated differences in physical and biological stress factors.

• Asian-Australasian Journal of Animal Sciences
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• 제19권3호
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• pp.418-424
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• 2006

This experiment was conducted to explore the efficacy of lacquer (Rhus verniciflua) supplementation on growth performance, nutrient digestibility, carcass traits and serum biochemical values in commercial broilers. Eight hundred and forty Hubbard broiler chicks (1d old) were randomly distributed and allotted to four dietary treatments for five weeks. Each treatment had 5 replicates with 42 birds each. The dietary treatments were (1) control (2) 1% lacquer, (3) 2% lacquer and 4) 4% lacquer supplied as meal in the diet. Supplementation with lacquer improved weight gain (p = 0.0960) showing a linear trend during the starter phase (0-3 wk), but weight gain, feed intake and feed conversion ratio remained unaffected at the finisher phase (4-5 wk) and overall (0-5 wk). The nutrient digestibility studies conducted after 18 and 35 days of experimental feeding showed a linear (p<0.05) increase in digestibility of CP and ether extract at both measurement timess. The carcass studies were conducted on two birds per replicate (10 per treatment) at the end of both 3 and 5 wk. The dressing percentage, liver weight, heart weight, abdominal fat and the breast meat expressed as a percentage of live weight, did not differ significantly due to treatments at both phases. The serum cholesterol and high-density lipoprotein (HDL) showed a linear decrease (p = 0.0683 and p = 0.0322, respectively) as the level of supplementation increased at 3 wk; at 5 wk serum cholesterol, HDL and triglyceride levels decreased significantly showing a positive linear effect of lacquer on fat metabolism. The meat color values such as lightness, redness and yellowness did not reveal any significant trend. Overall, it could be concluded that lacquer supplementation at higher levels did not affect growth performance, but had a positive impact on fat metabolism by influencing fat digestibility and reducing serum cholesterol and triglyceride.

• Asian-Australasian Journal of Animal Sciences
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• 제21권11호
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• pp.1659-1664
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• 2008

To study the working mechanisms for non-starch polysaccharidases to improve the growth performance of broiler chickens, a 21-day feeding trial was conducted. Two dietary treatments were included: 1) wheat diet (the control); 2) wheat+xylanase diet (xylanase, Allzyme PT, Alltech, Kentucky, USA). There were 8 replicates with 8 birds each for each treatment and the experimental diets were given to birds from hatch. Feed intake and body weight were measured on days 7 and 21. At the same ages, samples were taken for the determination of selected groups of luminal and mucosa-associated bacteria, mucosal morphology, brush-border membrane (BBM) bound enzyme activity and ileal nutrient digestibility. The xylanase supplement increased (p<0.05) body weight gain (BWG) and improved feed conversion ratio (FCR) at the end of the experiment but protein and starch digestibilities were not affected (p>0.05) by xylanase. Up to day 7, xylanase increased the counts of C. perfringens in the ileum and total anaerobic bacteria (TAB) in the caeca (p<0.05, p=0.07, respectively). By day 21, the counts of ileal lactobacilli (p<0.05) and TAB (p=0.07) were lower in birds given the xylanase-supplemented diet than in those on the control diet. No significant differences were observed in the counts of mucosa-associated lactobacilli and coliforms between xylanase treatment and the control at both ages. Villus height at the jejunum was not affected (p>0.05) by the supplement but crypt depth at the same site was reduced at day 7. Also, xylanase tended to increase the concentration of BBM protein (p = 0.09) and the specific activity of sucrase (p = 0.07) at day 21.