Tae Gyeom Kim;Jongsoo Kim;Joonhaeng Lee;Jisun Shin;Mi Ran Han;Jongbin Kim;Yujin Kim;Jae Hee Park
Journal of the korean academy of Pediatric Dentistry
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v.51
no.2
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pp.149-164
/
2024
This study compared the solubility, water absorption, dimensional stability, release of various ions (hydroxyl, calcium, sulfur, strontium, and silicon), and cytotoxicity of light-cured resin-modified pulp-capping materials. Resin-modified calcium hydroxide (Ultra-blendTM plus, UBP), light-cured resin-modified calcium silicate (TheraCal LCTM, TLC), and dual-cure resin-modified calcium silicate (TheraCal PTTM, TPT) were used. Each material was polymerized; solubility, 24-hour water absorption, and 30- day dimensional stability experiments were conducted to test its physical properties. Solubility was assessed according to the ISO 6876 standard, and 24 hours of water absorption, 30 days of dimensional stability were assessed by referring to the previous protocol respectively. Eluates at 3 and 24 hours and on 7, 14, and 28 days were analyzed according to the ISO 10993-12 standard. And the pH, Ion-releasing ability, cell proliferation rate, and cell viability were assessed using the eluates to evaluate biochemical characteristics. pH was measured with a pH meter and Ion-releasing ability was assessed using inductively coupled plasma atomic emission spectrometry (ICP-AES). Cell proliferation rate and cell viability were assessed using human dental pulp cells (hDPCs). The former was assessed by an absorbance assay using the CCK-8 solution, and the latter was assessed by Live and Dead staining. TPT exhibited lower solubility and water absorption than TLC. UBP and TPT demonstrated higher stability than TLC. The release of sulfur, strontium, calcium, and hydroxyl ions was higher for TLC and TPT than for UBP. The 28-day release of hydroxyl and silicon ions was similar for TLC and TPT. TLC alone exhibited a lower cell proliferation rate compared to the control group at a dilution ratio of 1 : 2 in cell proliferation and dead cells from Live and Dead assay evaluation. Thus, when using light-cure resin-modified pulp-capping materials, calcium silicate-based materials can be considered alternatives to calcium hydroxide-based materials. Moreover, when comparing physical and biochemical properties, TPT could be prioritized over TLC as the first choice.
This study investigated the hypothesis that the dentin bond strength of self-etching adhesive (SEA) might be improved by applying additional layer of bonding resin that might alleviate the pH difference between the SEA and the restorative composite resin. Two SEAs were used in this study; Experimental SEA (Exp, pH: 1.96) and Adper Prompt (AP, 3M ESPE, USA, pH: 1.0) In the control groups they were applied with two sequential coats In the experimental groups, after applying the forst coat of assigned SEAs, the D/E bonding resin of All-Bond 2 (Bisco Inc., USA, pH: 6.9) was applied as the intermediate adhesive. Z-250 (3M ESPE, USA) composite resin was built-up in order to prepare hourglass-shaped specimens . The microtensile bond strength (MTBS) was measured and the effect of the Intermediate layer on the bond strength was analyzed for each SEA using t-test. The fracture mode of each specimen was inspected using stereomicroscope and Field Emission Scanning Electron Microscope (FE-SEM). When D/E bonding resin was applied as the second coat, MTBS was significantly higher than that of the control groups . The incidence of the failure between the adhesive and the composite or between the adhesive and dentin decreased and that of the failure within the adhesive layer increased. According to the results , applying the bonding resin of neutral pH can increase the bond strength of SEAs by alleviating the difference in acidity between the SEA and restorative composite resin.
The environment in composting greenhouse is very different with the traditional greenhouse by biothermal energy and $CO_2$ concentration. This experiment aimed to investigate the environmental effects on the growth and development of tomato grown at composting greenhouse. The room temperature is not different between two greenhouses because of heating and ventilation, but the soil temperature in composting greenhouse is about 7$^{\circ}C$ to 15$^{\circ}C$ higher than that of traditional greenhouse. The emission concentration of ammonia gas is the highest, 117.3ppm, at the 6th day starting the digest, and were gradually lowered from 7th day, 11 became 15.7ppm at the 16th day. The concentration of $CO_2$ in composting greenhouse were 250 to 2000ppm higher than that of traditional greenhouse for 4 months starting digest. The growth and development of tomato grown at composting greenhouse was better than that of traditional greenhouse. The yield in composting greenhouse was also better than that of traditional greenhouse. The sugar contents of tomato grown at composting greenhouse became about 1 $^{\circ}$Brix higher than that of traditional greenhouse.
Agricultural landscape has many ecosystem service functions. However, the development of the horticulture complex has no consideration for environmental conservation. Therefore, we analyzed the priorities of ecosystem service functions required for the composition. The study was conducted in three stages. As a result of the first survey, 17 functions were selected to be improved. In the second survey, 12 functions were selected excluding 5 functions. Finally, 1. Measures for water purification, 2. Groundwater recharge plan, 3. Surface water storage space, 4. Flood control measures, 5. Vegetation diversity space, 6. Carbon emission reduction plan, 7. Aquatic insect habitat space, 8. Amphibian reptiles 9. Landscape and Waste Management, 10. Bird Species Space, 11. Heat Island Mitigation Plan, 12. Experience / Ecological Education Plan. We proposed the structure, capacity, flow rate, arrangement and form of the water treatment facility to improve water quality by improving the function. We proposed a reservoir space of 7-10% for groundwater recharge. The development of reservoir and storage facilities suitable for the Korean situation is suggested for the surface water storage and flood control measures. And proposed to secure a green space for the climate cycle. Proposed habitat and nutrient discharge management for biodiversity. We propose green area development and wetland development to improve the landscape, and put into the facilities for experiential education. The results of the research can be utilized for the development and improvement of the horticultural complex.
Seo, Jin-Ho;Lee, Richard sungbok;Ahn, Su-Jin;Park, Su-Jung;Lee, Myung-Hyun;Lee, Suk Won
The Journal of Korean Academy of Prosthodontics
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v.53
no.3
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pp.198-206
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2015
Purpose: We aimed to investigate the effect of combined various microgrooves and thermal oxidation on the titanium (Ti) and to evaluate various in vitro responses of human periodontal ligament cells (PLCs). Materials and methods: Grade II titanium disks were fabricated. Microgrooves were applied on titanium discs to have $0/0{{\mu}m}$, $15/3.5{{\mu}m}$, $30/10{{\mu}m}$, and $60/10{{\mu}m}$ of respective width/depth by photolithography. Thermal oxidation was performed on the microgrooves of Ti substrata for 3 h at $700^{\circ}C$ in air. The experiments were divided into 3 groups: control group (ST), thermal oxidation group (ST/TO), and combined microgrooves and thermal oxidation group (Gr15-TO, Gr30-TO, Gr60-TO). Surface characterization was performed by field-emission scanning microscopy. Cell adhesion, osteoblastic differentiation, and mineralization were analyzed using the bromodeoxyurdine (BrdU), Alkaline phosphatase (ALP) activity, and extracellular calcium deposition assays, respectively. Statistical analysis was performed using the oneway analysis of variance and Pearson's bivariate correlation analysis (SPSS Version 17.0). Results: In general, the combined microgrooves and thermal oxidation group (Gr15-TO, Gr30-TO, Gr60-TO) showed significantly higher levels compared with the control (ST) or thermal oxidation (ST-TO) groups in the BrdU expression, ALP activity, and extracellular calcium deposition. Gr60-TO group induced highest levels of cell adhesion and osteoblastic differentiation. Conclusion: Within the limitation of this study, we conclude that the Ti surface treatment using combined microgrooves and thermal oxidation is highly effective in inducing the cell adhesion andosteoblastic differentiation. The propose surface is also expected to be effective in inducing rapid and strong osseointegration of Ti oral implants.
Purpose: This study is designed to investigate the various impacts of different types of scaler tips such as cooper alloy base tip and the others on the surface roughness of teeth and implant by the method which is currently in clinical use. Materials and methods: Four different types of disc shaped porcelain, titanium, zirconia, and Type III gold alloy dental materials sized 15 mm diameter, 1.5 mm thickness were used for the experiment. Plastic hand curette (Group PS), cooper alloy new tip (Group IS), and stainless steel tip (Group SS) were used as testing appliances. A total of 64 specimens were used for this study; Four specimens for each material and appliance group. Surface roughness was formed with 15 degree angle in ultrasonic scaler tip and with 45 degree angle in hand curette of instrument tip and the specimen surface with 5 mm long, one horizontal-reciprocating motion per second for 30 seconds by 40 g force. To survey the surface roughness of each specimen, a field emission scanning electron microscope, an atomic force microscope, and a surface profiler were used. (Ra, ${\mu}m$). Results: According to SEM, most increased surface roughness was observed in SS group while IS groups had minimal roughness change. Measurement by atomic force microscope presented that the surface roughness of SS group was significantly greater than those of PS, IS and control groups in the type III gold alloy group (P<.05). IS group showed lesser surface roughness changes compared to SS group in porcelain and gold alloy group (P<.05). According to surface profiler, surface roughness of SS group showed greater than those of PS, IS and control groups and IS group showed lesser than those of SS group in all specimen groups. Type III gold alloy group had large changes on surface roughness than those of porcelain, titanium, zirconia (P<.05). Conclusion: The result of this study showed that newly developed copper alloy scaler tip can cause minimal roughness impacts on the surface of implant and dental materials; therefore this may be a useful alternative for prophylaxis of implant and restored teeth.
Kang, Youn Koo;Kang, Suk Won;Paek, Yee;Kim, Young Hwa;Jang, Jae Kyung;Ryou, Young Sun
Journal of Bio-Environment Control
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v.26
no.4
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pp.317-323
/
2017
In this study, the heating performance and the energy saving effect of the heat pump system using hot waste water(waste heat) of the thermal power plant discharged from a thermal power plant to the sea were analyzed. The greenhouse area was $5,280m^2$ and scale of the heat pump system was 120 RT(Refrigeration Ton), which was divided into 30 RT, 40 RT and 50 RT. The heat pump system consisted of the roll type heat exchangers, hot waste water transfer pipes, heat pumps(30, 40, 50 RT), a heat storage tank and fan coil units. The roll type heat exchangers was made of PE(Poly Ethylene) pipes in consideration of low cost and durability against corrosion, because hot waste water(sea water) is highly corrosive. And the heating period was 5 months from October to February. During the heating performance test(12 hours), the inlet water temperature of evaporator was changed from $32^{\circ}C$ to $26^{\circ}C$, and heat absorption of he evaporator was changed from 175 kW to 120 kW. The inlet water temperature of the condenser rose linearly from $15^{\circ}C$ to $50^{\circ}C$, and the heat release of condenser was reduced by 40 kW from 200 kW to 160 kW. And the power consumption of the heat pump system increased from 30 kW to 42 kW. When the inlet water temperature of condenser was $15^{\circ}C$, the heating COP(Coefficient Of Performance) was over 7.0. When it was $30^{\circ}C$, it dropped to 5.0, and when it was above $40^{\circ}C$, it decreased to less than 4.0. It was analyzed that the reduction of heating energy cost was 87% when compared to the duty free diesel that the carbon dioxide emission reduction effect was 62% by recycling the waste heat of the thermal power plant as a heat source of the heat pump system.
Purpose: To evaluate the effect of acupuncture on regional cerebral blood flow (rCBF) at acupoints suggested by oriental medicine to be related to the treatment of cerebrovascular diseases. Materials and Methods: Rest/acupuncture-stimulation Tc-99m ECD brain SPECT using a same-dose subtraction method was performed on 54 normal volunteers (34 males, 20 females, age range from 18 to 62 years) using six paradigms: acupuncture at acupoints GV. 20, GV. 26, LI. 4, ST. 36 and SP. 6. In the control study, needle location was chosen on a non-meridian focus 1 cm posterior to the right fibular head. All images were spatially normalized, and the differences between rest and acupuncture stimulation were statistically analyzed using SPM$^{(R)}$ for Windows$^{(R)}$. Results: Acupuncture applied at acupoint GV. 20 increased rCBF in both the anterior frontal lobes, the right frontotemporal lobes, and the left anterior temporal lobe and the left cerebellar hemisphere. Acupuncture at GV 26 increased rCBF in the left prefrontal cortex. Acupuncture at LI. 4 increased rCBF in the left prefrontal and both the inferior frontal lobes, and the left anterior temporal lobe and the left cerebellar hemisphere. Acupuncture at ST. 36 increased rCBF in the left anterior temporal lobe, the right inferior frontal lobes, and the left cerebellum. Acupuncture at SP. 6 increased rCBF in the left inferior frontal and anterior temporal lobes. In the control stimulation, no significant rCBF increase was observed. Conclusion: The results demonstrated a correlation between stimulation at each acupoint with increase in rCBF to the corresponding brain areas.
Purpose: To localize and compare the neural basis of verbal and visual human working memory, we performed functional activation study using $H_2^{15}O$ PET. Materials and Methods: Repeated $H_2^{15}O$ PET scans with one control and three different activation tasks were performed on six right-handed normal volunteers. Each activation task was composed of 13 match-ing trials. On each trial, four targets, a fixation dot and a probe were presented sequentially and subject's task was to press a response button to indicate whether or not the probe was one of the previous targets. Short meaningful Korean words, simple drawings and monochromic pictures of human faces were used as matching objects for verbal or visual memory. All the images were spatially normalized and the differences between control and activation states were statistically analyzed using SPM96. Results: Statistical analysis of verbal memory activation with short words showed activation in the left Broca's area, promoter cortex, cerebellum and right cingulate gyrus. In verbal memory with simple drawings, activation was shown in the larger regions including where activated with short words and left superior temporal cortex, basal ganglia, thalamus, prefrontal cortex, anterior portion of right superior temporal gyrus and right infero-lateral frontal cortex. On the other hand, the visual memory task activated predominantly right-sided structures, especially inferior frontal cortex, supplementary motor cortex and superior parietal cortex. Conclusion: The results are consistent with the hypothesis of the laterality and dissociation of the verbal and visual working memory from the invasive electrophysiological studies and emphasize the pivotal role of frontal cortex and cingulate gyrus in working memory system.
Kim, Hye-Young;Kwun, Hyun-Sook;Song, Keun-Bae;Hong, Suk-Jin
Journal of Korean society of Dental Hygiene
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v.2
no.2
/
pp.175-186
/
2002
Fluoride has been one of the most widely studied caries-preventive agents. But the effect of prenatal administration had been controversies for many years. The results showed that there were no influence on reproductive rate of rats with administration of fluoride from 0 to 20 ppm during pregnancy(p>0.05). There was a trend towards slightly increased the mean ash weight in the 1, 5 and 20 ppm groups, as compared with the control group. However, there was no significant differences among groups (p>0.05). The contents of calcium, magnesium and phosphorus in the total bone were increased with the administrated fluoride concentration were increased, but there were no statistically significant differences among groups(p>0.05). The mean fluoride level of 1 ppm group was significantly higher than that of control group, but the concentrations of fluoride in total carcass pups of 5 and 20 ppm groups were significantly less than that of 1 ppm group(p>0.05). The results of this study indicate that the amount of fluoride transferred to the offspring, which may produce anticariogenic effects in the primary teeth of their effects in the primary teeth of their offspring.
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