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Kumar, Nitish;Reddy, M.P.
- Journal of Forest and Environmental Science
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- v.27 no.2
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- pp.61-72
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- 2011
Micropropagation is an alternative mean of propagation that can be employed in mass multiplication of plants in relatively shorter time. Recent modern techniques of propagation have been developed which could facilitate large scale production of true-to-type plants and for the improvement of the species using genetic engineering techniques in the next century. An overview on the in vitro propagation via meristem culture, regeneration via organogenesis and somatic embryogenesis is presented. The usefulness of the plants in commercial industry as well as propagation techniques, screening for various useful characteristics and the influence of different cultural conditions in the multiplication, rooting and acclimatization phases on the growth of tissue cultured plant discussed.
https://doi.org/10.7747/JFS.2011.27.2.1 인용 PDF KSCI

Kumar, Nitish;Singh, Amritpal S.;Modi, Arpan R.;Patel, Armi R.;Gajera, Bhavesh B.;Subhash, Narayanan
- Journal of Forest and Environmental Science
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- v.26 no.1
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- pp.31-36
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- 2010
Sixteen microsatellite markers (simple sequence repeat (SSR) markers) were employed to examine the genetic stability of 27 randomly chosen date palm (Phoenix dactylifera L.) plants produced through somatic embryogenesis with upto forty two in vitro subcultures. No microsatellite DNA variation was observed among all micropropagated plants. Our results indicate that the micropropagation protocol used for rapid in vitro multiplication is appropriate and suitable for clonal propagation of date palm and corroborated that somatic embryogenesis can also be used as one of the safe modes for production of true-to-type plants of date palm. This is the first report on the use of microsatellite DNA markers to establish the genetic stability in micropropagated date palm plants.
https://doi.org/10.7747/JFS.2010.26.1.031 인용 PDF KSCI

Hui Yeong Jeong;Ji Ah Kim
- Proceedings of the Plant Resources Society of Korea Conference
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- 2021.04a
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- pp.51-51
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- 2021
This study was conducted to develop a somatic embryogenesis protocol for the Cnidium officinale Makino difficult to seed propagation. The immature flowers were used as explants. The concentration of a 2,4-D 1.0mg/L was found to be optimal concentration for induction of embryogenic callus and somatic embryos. Addition of 0.3mg/L, 0.5mg/L and 1.0mg/L to the embryo germination medium promoted somatic embryo germination. Among four concentrations, GA₃ 1.0mg/L were superior to others. Shoots were transferred to hormone-free MS medium after 2 months of culture in the dark. We obtained an optimized protocol for the regeneration of C. officinale.
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