• Title/Summary/Keyword: Embryo Development

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Development of Artificial Seeds by Encapsulation Zygotic Embryo of Apple (Malus pumila var. domestica) (사과의 접합자배 encapsulation에 의한 인공종자 개발)

  • Choi, Pil Son
    • Korean Journal of Plant Resources
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    • v.34 no.1
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    • pp.59-63
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    • 2021

  • To study for germination rate of apple artificial seeds encapsulated with 2% alginic acids, the rates of germination for artificial seeds of various embryos with partial elimination of cotyledon measured by adding 80 mg/L gibberellic acid or/and nutritional media. The germination rate of artificial seeds produced embryo with 3/4 cotyledon (3/4 CE), embryo with 1/2 cotyledon (1/2 CE), embryo with 1/4 cotyledon (1/4 CE), and embryo without cotyledon (NCE) were gradually decreased with compare to normal embryo, but it's considerably recovered on the alginic acid containing MS solution or/and 80 mg/L GA3. In special, the low frequency (55.81%) of germination for artificial seeds of 1/2 cotyledon embryo with alginic acid was increased by treatment with combination of alginic acid, 3% sucrose, and 80 mg/L gibberellic acid (98.74%). In case of embryo without cotyledon, the low frequency of embryo without cotyledon (24.44%) in alginic acid almost recovered to 82.45% in alginic acid with MS solution and 80 mg/L gibberellic acid. The results shown that the germination of embryo was decreased by the removal of cotyledon, and demonstrated that the low rate of germination could be considerably increased by adding GA3 (80 mg/L) or/and nutrition.

  • https://doi.org/10.7732/kjpr.2021.34.1.059 인용 PDF KSCI

Studies on the Merclonal Protocorm of Orchild (IV) Protocorm development from seed embryo (양란의 생장점배양에 관한 연구)

  • 한창열
    • Journal of Plant Biology
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    • v.13 no.1
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    • pp.65-69
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    • 1970

  • Several days after culture, the parts around suspensor turned brown. In about 10 days the embryo started to form protocorm sending out hairs through seed coat. Around 20 days after culture, most of the protocorms emerged out of seed coat and some of them began to take green color. When observed two months after culture, the protocorn took the characteristic top-shape feature.

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