• Korean Journal of Animal Reproduction
• /
• 제21권3호
• /
• pp.265-274
• /
• 1997

The purpose of this study was attempted to investigate the a, pp.arences of healthy or artretic follicles in ovaries following repeats of pregnant mare serum gonadotropin(PMSG) treatments for superovulation in nulliparous rats. Thirty two rats(Sprague-Dawely, about 200-250 gm) were randomized into 4 groups. Control group rats were sacrified at estrus phase confirmed by vaginal smear. PMSG-treated group 1 rats, PMSG-treated group 2 rats and PMSG-treated group 3 rats were sacrified at 48 hrs after injection once with PMSG 25 IU, after 2 repeated injection by a week interval, and 3 repeated injection, respectively. The ovaires of rats were removed and then sections by paraffin embedding were stained with H-E or immunohistochemical staining using proliferating cell nuclear antigen monoclonal antibody (PCNA m Ab) and apoptotic kit. The criteria of follicle classification was based as small follicles with preantral follicles with 2~4 layers of granulosa cells surrounding the oocyte, as secondary follicles with more than 5 layers of granulosa cells and early signs of antral cavity or with small clefts on either side of the oocytes, and as tirtiary follicles with a single medium sized antral cavity or large well-formed antral cavity, respectively. The proportions of atretic follicles from small and middle follicles in immunohistochemical staining using PCNA m Ab were 17.9% and 21.3% in control group, 15.5% and 23.5% in PMSG-treated group 1, 24.3% and 26.7% in PMSG-treated group 2, 18.1% and 30.2% in PMSG-treated group 3, respectively. Groups with atretic follicles of higher proportion were ordered as PMSG-treated group 3, PMSG-treated group 2, PMSG-treated group 1 and control group. The proportions of positive cells in small, middle and large follicles were 31.1%, 33.5% and 28.5% respectively. The follicles with positive cells of higher proportion were ordered middle, small and large follicles. In immunohischemical staining using apoptotic kits, small follicles in all 4 groups did not contain positive cells, and proportions of atretic follicles from middle and large follicles were 24.9, 30.7, 33.8 and 40.1% in control, PMSG-treated gruop 1, PMSG-treated group 2 and PMSG-treated group 3, respectively. These results suggested that repeats of PMSG treatment increased proportion of atretic follicles in ovaries, and middle follicles are more quickly developing than small or large follicles.

• Journal of KIISE:Computer Systems and Theory
• /
• 제33권1_2호
• /
• pp.79-91
• /
• 2006

The intelligent disk can improve the overall performance of the I/O subsystem by processing the I/O operations in the disk side. At present time, however, realizing the intelligent disk seems to be impossible because of the limitation of the I/O subsystem and the lack of the backward compatibility with the traditional I/O interface scheme. In this paper, we proposed new model for the intelligent disk that dynamically optimizes the I/O subsystem using the information that is only related to the physical sector. In this way, the proposed model does not break the compatibility with the traditional I/O interface scheme. For these works, the boosting algorithm that upgrades a weak learner by repeating teaming is used. If the last learner classifies a recent I/O workload as the multimedia workload, the disk reads more sectors. Also, by embedding this functionality as a firmware or a embedded OS within the disk, the overall I/O subsystem can be operated more efficiently without the additional workload.

• Applied Microscopy
• /
• 제37권3호
• /
• pp.167-174
• /
• 2007

Heat shock protein (HSP) 70 functions as a molecular chaperon and reduces stress-induced denaturation and aggregation of intracellular proteins. Erythropoietin (EPO) plays an important role during acute renal failure repair process by rapidly correcting anemia and enhancing renal tubular regeneration. The purpose of this study was to examine the effect of EPO treatment on renal HSP70 expression. Male Sprague-Dawley rats were injected rHUEPO. Kidney were preserved by in vivo perfusion with paraformaldehyde-lysine-periodate (PLP) and processed for immunohistochemistry and electron microscopy. In control kidney, HSP70 was expressed in the cortex, outer medulla and inner medulla. Especially, HSP immunoreactiviy was mainly founded in descending thin limb of outer medulla and inner medullary collecting duct. In EPO treated kidney, HSP70 expression markedly increased in the descending thin limb of outer medulla and newly detected in cortical collecting duct. Electron microscopy showed the presence of HSP immunoreactivity on the intracelluar vesicles and Golgi complex of descending thin limb and cortical collecting duct. These findings suggest that EPO treatment leads to new production of HSP70 in renal tubular cells, and induction of HSP70 by rHuEPO is causally related to protective function.

• The Journal of the Acoustical Society of Korea
• /
• 제39권5호
• /
• pp.461-467
• /
• 2020

In noisy and multi-speaker environments, the performance of speech recognition is unavoidably lower than in a clean environment. To improve speech recognition, in this paper, the signal of the speaker of interest is extracted from the mixed speech signals with multiple speakers. The VoiceFilter model is used to effectively separate overlapped speech signals. In this work, clustering by Probabilistic Linear Discriminant Analysis (PLDA) similarity score was employed to detect the speech signal of the interested speaker, which is used as the reference speaker to VoiceFilter-based separation. Therefore, by utilizing the speaker feature extracted from the detected speech by the proposed clustering method, this paper propose a speaker diarization system using only the mixed speech without an explicit reference speaker signal. We use phone-dataset consisting of two speakers to evaluate the performance of the speaker diarization system. Source to Distortion Ratio (SDR) of the operator (Rx) speech and customer speech (Tx) are 5.22 dB and -5.22 dB respectively before separation, and the results of the proposed separation system show 11.26 dB and 8.53 dB respectively.

• Korean Journal of Veterinary Research
• /
• 제39권3호
• /
• pp.455-462
• /
• 1999

This study was designed to investigate the number of the growing and mature follicles in each stage of estrus cycle in mature rats. Eighteen mature rats(Sprague-Dawley, initially 190~230gm) were randomly alloted into 4 groups(proestrus, estrus, metestrus, and diestrus) according to estrus cycles. The uteri and ovaries of rats were collected and then alternative sections of paraffin embedding ovaries were stained with H-E. Numbers of large, middle and small follicles or only large and middle follicles from secondary and tertiary follicles were investigated by LM photography of preparations. Small follicles were defined as secondary follicles with 2~5 cell layers of granulosa cells surrounding the oocyte, and middle follicles were defined as secondary follicles with more than 5 cell layers or with early signs of antral cavity or with more than one small cleft on either side of the oocytes and large follicles were defined as tertiary follicles with a single medium or large antral cavity. The number of follicles in a pair ovary per rat was appeared to be ranged from 207 to 370 and the mean number of these follicles was $270.4{\pm}52.6$ and the mean number of follicles per ovary was $134.9{\pm}32.0$. The mean number of large, middle and small follicles per ovary was appeared to be $16.4{\pm}4.4$($12.2{\pm}3.3%$), $36.2{\pm}8.6$($26.8{\pm}6.4%$), and $82.7{\pm}24.0$($61.3{\pm}17.8%$), respectively. The mean number of large and middle follicles in each stage group of estrus cycle was appeared to be $17.8{\pm}2.1$ and $38.3{\pm}7.4$ at proestrus stage group, $15.7{\pm}5.2$ and $38.0{\pm}10.0$ at estrus stage group, $16.5{\pm}3.5$ and $33.8{\pm}7.0$ at metestrus stage group, $16.7{\pm}5.8$ and $29.7{\pm}5.5$ at diestrus group, respectively. In histological findings of large follicles during each estrus cycle, the large follicles in proestrus group contain single small antrum, thick granulosa cell layers, and were $300{\sim}500{\mu}m$ in diameter and were growing follicles with PCNA-positive cells in the granulosa cell layers, and other luteinizing follicles of proestrus cycle stage were decreased in size and were thicker in wall thickness and more luteinized than those in metestrus and diestrus stage groups. The large follicles in estrus stage group contain thick granulosa cell layers and nonprominent cumulus-oocyte complexes in antrum, and were $400{\sim}700{\mu}m$ in diameter and were growing follicles with PCNA-positive cells in the granulosa cell layers. The large follicles in metestrus and diestrus stage groups contain enlarged antrums, thinner layers of walls and prominent cumulus-oocyte complexes, and were $700-950{\mu}m$ in diameter, and were nongrowing follicles without PCNA-positive cells or another large follicles contain cells with dark stainability and distinct boundary.

• Journal of the Korea institute for structural maintenance and inspection
• /
• 제20권1호
• /
• pp.41-47
• /
• 2016

This paper presents a bond test to find the effect of shear key and edge length from the bonded FRP in near surface-mounted(NSM) retrofit using FRP plate. Main parameters in the test are the location and size of shear key and the edge length. For the test, 10 specimens were made by embedding FRP plate of $3.6mm{\times}16mm$ into $400mm{\times}200(300)mm{\times}400mm$ concrete block and fixing it by using epoxy. Tensile load was applied to the FRP of the specimens until failure and was recorded at each load increase. In addition, the bond slip and elongation of FRP were measured during the test. From the test, it was found that the further the shear key located from the loading, the higher strength we could get. The bond strength inversely depended on the size of shear key. Especially, when the size of shear key was to be lagger than certain size, the bond strength decreased to very low value; even less than that of the case without shear key. The bond strength somewhat increased corresponding to the increase of edge length from the bonded end of FRP to loading in spite of same bond length. The bond-slip between FRP and concrete governed overall deformation in the bond test of NSM FRP so that the effect of excessive slip is necessary to be considered in the design.

• Journal of the Institute of Electronics Engineers of Korea CI
• /
• 제42권6호
• /
• pp.83-92
• /
• 2005

This paper presents a watermarking approach that the 1-level Discrete Wavelet Transform(DWT) coefficients of a $64{\ast}64$ binary logo image as watermarks are inserted in LL band and other specific frequency bands of the host image using Multi-Resolution Analysis(MRA) Wavelet transform for copyright protection of image data. The DWT coefficients of the binary logo image are inserted in blocks of LL band and specific bands of the host image that the 3-level DWT has been performed in the same orientation. We investigate Significant Coefficients(SCs) in each block of the frequency areas in order to prevent the quality deterioration of the host image and the watermark is inserted by SCs. When the host image is distorted by difference of the distortion degree in each frequency, we set the thresholds of SCs on each frequency and completely insert the watermark in each frequency of the host image. In order to be invisibility of the watermark, the Human Visual System(HVS) is applied to the watermark. We prove the proper embedding method by experiment. Thereby, we rapidly detect the watermark using this watermarking method and because the small size watermarks are inserted by HVS and SCs, the results confirm the superiority of the proposed method on invisibility and robustness.

• Journal of the Institute of Electronics Engineers of Korea CI
• /
• 제49권2호
• /
• pp.123-133
• /
• 2012

This paper discuss about DNA watermarking using coding DNA sequence (CDS) for the authentication, the privacy protection, or the prevention of illegal copy and mutation of DNA sequence and propose a DNA watermarking scheme with the mutation robustness and the animo acid preservation. The proposed scheme selects a number of codons at the regular singularity in coding regions for the embedding target and embeds the watermark for watermarked codons and original codons to be transcribed to the same amino acids. DNA base sequence is the string of 4 characters, {A,G,C,T} ({A,G,C,U} in RNA). We design the codon coding table suitable to watermarking signal processing and transform the codon sequence to integer numerical sequence by this table and re-transform this sequence to floating numerical sequence of circular angle. A codon consists of a consecutive of three bases and 64 codons are transcribed to one from 20 amino acids. We substitute the angle of selected codon to one among the angle range with the same animo acid, which is determined by the watermark bit and the angle difference of adjacent codons. From in silico experiment by using HEXA and ANG sequences, we verified that the proposed scheme is more robust to silent and missense mutations than the conventional scheme and preserve the amino acids of the watermarked codons.

• Composites Research
• /
• 제28권4호
• /
• pp.155-161
• /
• 2015

A composite structure was fabricated with embedded impact detection capabilities for applications in Structural Health Monitoring (SHM). By embedding sensor functionality in the composite, the structure can successfully perform impact localization in real time. Smart resin, composed of $Pb(Ni_{1/3}Nb_{2/3})O_3-Pb(Zr,\;Ti)O_2$ (PNN-PZT) powder and epoxy resin with 1:30 wt%, was used instead of conventional epoxy resin in order to activate the sensor function in the composite structure. The embedded impact sensor in the composite was fabricated using Hand Lay-up and Vacuum Assisted Resin Transfer Molding(VARTM) methods to inject the smart resin into the glass-fiber fabric. The electrodes were fabricated using silver paste on both the upper and bottom sides of the specimen, then poling treatment was conducted to activate the sensor function using a high voltage amplifier at 4 kV/mm for 30 min at room temperature. The composite's piezoelectric sensitivity was measured to be 35.13 mV/N by comparing the impact force signals from an impact hammer with the corresponding output voltage from the sensor. Because impact sensor functionality was successfully embedded in the composite structure, various applications of this technique in the SHM industry are anticipated. In particular, impact localization on large-scale composite structures with complex geometries is feasible using this composite embedded impact sensor.

• Biomedical Science Letters
• /
• 제25권4호
• /
• pp.426-430
• /
• 2019

Currently, molecular diagnostic assays based on nucleic acid amplification tests have been shown to effectively detect mycobacterial infections in various types of specimen, however, variable sensitivity was shown in FFPE samples according to the kind of commercial kit used. The present study therefore used automated PCR-reverse blot hybridization assay (REBA) system, REBA Myco-ID HybREAD 480^®, for the rapid identification of Mycobacterium species in various types of human tissue and compared the conventional one-tube nested-PCR assay for detecting Mycobacterium tuberculosis (MTB). In conventional nested-PCR tests, 25 samples (48%) were MTB positive and 27 samples (52%) were negative. In contrast, when conducted PCR-REBA assay, 11 samples (21%) were MTB positive, 20 samples (39%) were NTM positive, 8 samples (15%) were MTB-NTM double positive, and 13 samples (25%) were negative. To determine the accuracy and reliability of the two molecular diagnostic tests, the one-tube nested-PCR and PCR-REBA assays, were compared with histopathological diagnosis in discordant samples. When conducted nested-PCR assay, 10 samples (59%) were MTB positive and seven samples (41%) were negative. In contrast, when conducted PCR-REBA test, three samples (17%) were MTB positive, 10 samples (59%) were NTM positive and four samples (24%) were negative. In conclusion, the automated PCR-REBA system proved useful to identify Mycobacterium species more rapidly and with higher sensitivity and specificity than the conventional molecular assay, one-tube nested-PCR; it might therefore be the most suitable tool for identifying Mycobacterium species in various types of human tissue for precise and accurate diagnosis of mycobacterial infection.