• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.404-405
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• 2000

• Journal of Korean Society of Forest Science
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• v.95 no.1
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• pp.32-37
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• 2006

The influence of different doses of jasmonic acid on both biomass of adventitious roots and accumulation of secondary metabolite in bioreactor cultures of Eleutherococcus koreanum was studied. The maximum growth (2.9 g Dry Weight/L) of adventitious roots was observed in the absence of jasmonic acid. The biomass of adventitious roots decreased with the increase in jasmonic acid dosage. High level of jasmonic acid efficiently stimulated the production of both eleutheroside B and E. The highest eleutheroside B ($49.0{\mu}g/g\;DW$) was observed at 0.4 mg/L jasmonic acid. Jasmonic acid at 0.2 mg/L led to the maximum accumulation of eleutheroside E ($64.7{\mu}g/g\;DW$). Whereas eleutheroside $E_1$ was maximally $19.0{\mu}g/g\;DW$ accumulated in the absence of jasmonic acid, the total eleutheroside ($259.9{\mu}g/L$) was the highest when 0.01 mg/L jasmonic acid was treated. In this study, the level of eleutheroside B decreased whereas the maximum levels of eleutheroside E and eleutheroside $E_1$ were observed at 10th and 4th day after jasmonic acid treatment.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.187-187
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• 2005

• Korean Journal of Medicinal Crop Science
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• v.4 no.4
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• pp.333-339
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• 1996

The root and stem bark of Eleutherococcus spp. is used for the treatment of paralysis, rheumatism, arthritis, diabetes, stamina failure, high blood pressure, etc. This study was con­ducted to identify botanical origins and to select standard variety of Eleutherococcus spp. with analyzing eleutheroside E content(%) of root bark and that of Eleutherococcus senticosus at different collected area, time, part and grown area. The content of eleutheroside E in the root bark was determined by HPLC, and it was 0.1925% in Eleutherococcus senticosus, 0.1103% in E. chiisanensis, 0.0348% in E. koreanum and 0.0236% in E. sieboldianum. In seasonal change of eleutheroside E content, it was higher in autumn product than summer product in Eleutherococcus senticosus. It also was higher in root bark than stem bark in different part. To investigate eleutheroside E content of root bark among different collected local area in E. sen­ticosus, it was high order to Mt. Deokyu, Hokkaido and Mt. Seolak as a 0.1625%. 0.0493% and 0.027 %, respectively. The growth of Eleutherococcus senticosus was good at grown Unbong (altitude 10m), but eleu­theroside E content was high at grown Nogodan (altitude 1, 450m).

• Horticultural Science & Technology
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• v.28 no.4
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• pp.678-684
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• 2010

In an attempt to improve biomass and bioactive compound production, we cultured adventitious roots of $Eleutherococcus$ $koreanum$ in 250 mL Erlenmeyer flasks using Murashige and Skoog (MS) medium with different concentrations of auxins (IBA, NAA, IAA) and cytokinins (BA, kinetin, TDZ). Root biomass (fresh and dry weight) was enhanced at $5mg{\cdot}L^{-1}$ indole-3-butyric acid (IBA) after 5 weeks of culture. The content of total phenolics and flavonoids was also increased with $5mg{\cdot}L^{-1}$ IBA compared to ${\alpha}$-naphtalene acetic acid (NAA) or indole-3-acetic acid (IAA) treatments. The combination of $5mg{\cdot}L^{-1}$ IBA with $0.1mg{\cdot}L^{-1}$ thidiazuron (TDZ; N-phenyl-N'-1,2,3,-thidiazol-5-ylurea) enhanced root fresh and dry weight (1.4- and 1.6-fold, respectively) as well as the content of total phenolics and flavonoids compared to the relative control (without cytokinin). On the contrary, $N_6$-benzyladenine (BA) and 6-furfurylaminopurine (kinetin) did not significantly affect root biomass and bioactive compound production in adventitious roots of $E.$ $koreanum$. These results suggested that $5mg{\cdot}L^{-1}$ IBA combination with $0.1mg{\cdot}L^{-1}$ TDZ supplementation was most suitable for both biomass and bioactive compound production from adventitious roots of $E.$ $koreanum$.

• Journal of Plant Biotechnology
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• v.32 no.1
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• pp.57-61
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• 2005

The influence of different growth regulators on biomass of adventitious roots and secondary metabolites accumulation in bioreactor cultures of Eleutherococcus koreanum was studied. The maximum growth of adventitious roots was observed in the presence of $2.0\;\cal{mg/L}$ IBA (6.7 g DW/L). However the productivity of eleutheroside E was not significantly different among IBA levels. High level of thidiazuron (TDZ) efficiently increased both root growth and secondary metabolites production. Especially when $0.1\;\cal{mg/L}$ TDZ was combined with $3.0\;\cal{mg/L}$ IBA, the highest eleutheroside E accumulation ($3,327{\mu}g/g $DW) was observed. When the same TDZ level was treated in combination with $5.0\;\cal{mg/L}$ IBA, both chlorogrnic acid and eleutheroside B were accumulated to maximum level. In contrast, zeatin remarkably suppressed the accumulation of both eleutheroside B and chlorogenic acid.

• Journal of Korean Society of Forest Science
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• v.98 no.4
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• pp.363-369
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• 2009

This study was carried out to investigate the effect of ${NO_3}^-$ and ${NH_4}^+$ on the adventitious root growth and eleuthroside synthesis of Eleutherococcus koreanum in 5 L-bioreactor culture. The change in the medium components was also measured during culture. The fresh weignt of adventitious root reached to the highest level of 30.8 g FW/L in the presence of both 50 mM ${NO_3}^-$ and 10 mM $NH_4^+$, representing 3.6-fold increase compared to the 60 mM ${NH_4}^+$ alone. However, as the increase of the portion of ${NH_4}^+$, the root growth was decreased. However, the maximum eleutheroside B, E and E1 contents were $57.3{\mu}g/g$ DW, $188.4{\mu}g/g$ DW and $47.3{\mu}g/g$ DW, with 30 mM, 60 mM and 15 mM total nitrogen source, respectively. Fresh weight of adventitious root increased up to 6.8-fold of inoculum size within 9 weeks. The amounts of ${NH_4}^+$, $K^+$, ${NO_3}^-$ and ${PO_4}^-$ were decreased during culture periods. Based on these results, we suggest that various further studies are required to increase the biomass and the useful secondary metabilites.