• 한국임상수의학회지
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• 제37권3호
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• pp.130-134
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• 2020

This study aimed to confirm the effects that the mobile phone has on Electroretinogram (ERG). The ERG responses of three groups of healthy dogs, five in each group, were studied. ERG test was performed consecutively before and after the mobile phone was carried out. For group A, music was played with the sound on; for group B, music was played but the sound off; and for group C, the phone was set on the airplane mode. In the presence of a mobile phone, the amplitudes of a- and b-wave were increased in all groups. The a- and b-wave amplitudes at the flash intensity of 3,000 mcd·s/㎡ were significantly increased in all groups (p < 0.05). Based on the results, it is recommended to conduct ERG test in the absence of a mobile phone for the accurate evaluation of the retinal function.

• 대한임상검사과학회지
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• 제43권2호
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• pp.33-42
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• 2011

Zinc plays a key role in genetic expression, cell division, and cell growth and is essential for the functions of more than 450 metalloenzyme. There are high concentrations of zinc in pigment epithelium in bullfrog eye. Zinc deficiency causes night blindness and abnormal dark adaptation. The purpose of this study was to identify ERG (electroretinogram) b-wave sensitivity during light and dark adaptation in bullfrog retina after zinc and zinc chelators treatment such as histidine and TSQ (N-(6-methoxy-8-qunolyl)-p-toluenesulfon amide). Especially, we focused whether histidine act as a zinc chelator in the Muller cell. The results of our study are summarized as follows: 1) Both zinc and histidine elevated ERG b-wave amplitude and threshold in Muller cells by accelerating rhodopsin regeneration time and increased a-peak absorbance during light adaptation. 2) TSQ reduced those by prolonging rhodopsin regeneration time and decrement of a-peak absorbance during light adaptation. 3) Zinc shortened rhodopsin regeneration time and prolonged a-peak absorbance. These results suggested that histidine may act as a zinc-mediated transporter in presynaptic Muller cell membrane rather than zinc chelator and acts as a GABA-receptor inhibitor which blocks $Cl^-$ influx to the postsynapse.

• Journal of Photoscience
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• 제2권2호
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• pp.63-67
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• 1995

Zinc plays a key role in genetic expression, cell division, and growth and is essential for the function of more than 200 enzymes; effects of zinc deficiency induce many syndromes, including abnormal visual adaptation. The pigment epithelium (EP) contains high concentrations of zinc in humans and in animals and it participates in threshold elevation, visual sensitivity increment, and acceleration of rhodopsin regeration during visual adaptation. The origin of c-wave of electroretinogram(ERG) is not only pigment epithelium as shown in present research, but also other cell layers, perhaps the photoreceptors. We propose zinc as a candidate for an internal messenger which participates in signal amplification.

• Journal of Photoscience
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• 제3권3호
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• pp.127-132
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• 1996

Calcium has a variety of functions in neuron and muscle cells and blood clotting, especially in the visual system where dark adapted rods cotransport with Na$^+$ into the cell. An influx of Ca$^{2+}$ flows out of the cell through the Na$^+$ - Ca$^{2+}$ exchanger. By using a modified Ussing chamber in order to bring in vivo environment close, we have concluded that Ca$^{2+}$ blocks the activity of guanylate cyclase; in consequence, having an effect on the amplitude of electroretinogram (ERG). We suggest that Ca$^{2+}$ moves between the photoreceptor and the vitreous humor by way of certain Ca$^{2+}$ transport mechanisms. Also, the effect of Zn$^{2+}$ in Ca$^{2+}$ - free ringer solution caused an elevation of amplitude in ERG and a reduction of threshold.

• 한국안광학회지
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• 제12권1호
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• pp.23-33
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• 2007

안구 조직에는 많은 양의 아연과 타우린이 존재하며 특히 망막에서 맥락막에 이르는 부위에서의 분포가 두드러지고, 이는 아연과 타우린이 이들 조직에서 중요한 역할을 담당하리라는 것을 시사한다. 또한 시각 감수성에 영향을 미치는 또 다른 인자로서, 온도에 대한 연구들이 보고되고 있다. 그러나 지금까지의 많은 연구에도 불구하고 시각 순응계에서의 이들의 기능이나 상호작용은 명확히 알려져 있지 않다. 본 연구에서는 변온동물의 일종인 황소개구리(bullfrog, Rana catesbeiana)의 안구를 이용하여 적정 농도의 아연과 타우린의 처리와 저온효과에 따른 망막전위도(ERG)와 흡수 스펙트럼의 변화를 관찰함으로써, 이들 인자들이 시각 감수성과 시각 순응에 미치는 효과를 알아보고자 하였다. 아연과 타우린의 효과를 알아보기 위한 이들의 적정 농도는 각각 10-4 M과 10-5 M로 결정하였고, 저온효과를 알아보기 위한 온도 변화 과정은'$25^{\circ}C {\rightarrow}0^{\circ}C{\rightarrow}25^{\circ}C$'를 거치게 하였다. 적정 농도의 아연과 타우린의 처리와 저온효과에 의해 망막전위도 실험 결과 역치(threshold)와 망막전위도 b-파의 크기가 증가하였고, 흡수 스펙트럼 분석 결과 400~750 nm 범위에서의 흡광도 증가를 볼 수 있었다. 또한 아연과 타우린, 그리고 아연과 저온효과 간에는 상승효과가 있음을 확인 할 수 있었다.

• Journal of Photoscience
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• 제6권2호
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• pp.77-83
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• 1999

Calcium has a variety of functions in neuron and muscle cells and blood clotting, especially in the visual system where dark adapted rods cotransport with Na$\^$+/ into the cell. An influx of Ca$\^$++/ flows out of the cell through the Na$\^$+/-Ca$\^$++/ exchanger. By using a modified Using chamber in order to bring in vivo environment close, we have known that Ca$\^$++/ blocks the activity of guanylate cyclase, in consequence, having an effect on the amplitude of electroretinogram (ERG). We have measured the Ca$\^$++/, K$\^$+/, and Na$\^$+/ concentration in dark and light adapted bullfrog＇s (Rana catesbeiana) vitreous humor. The calcium concentration of the light adapted bullfrog＇s vitreous humor was higher than that of the dark adapted bullfrog＇s vitreous humor This means that ion activity between the photoreceptor and vitreous humor side is light dependent and we have found that a Ca$\^$++/ channel and Ca$\^$++/K$\^$+/ exchanger exist in the vitreous humor side. Taken together permeability of Ca$\^$++/, K$\^$+/ and K$\^$+/ ion internal limiting membrane faced in the vitreous humor side has light-dependent activity during the illumination.

• 수산해양기술연구
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• 제51권1호
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• pp.102-110
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• 2015

The characteristics of aquatic light fields are generally reflected in the visual systems of fishes inhabiting them. Therefore, research on light sensitivity of fish is useful to explain the correlation between the visual function and habitat, behavior and distribution of fish. Rockfish is an important species in coastal ecology and also one of the main species for culturing in Korea. To make a contribution on the maintenance of the fish resources and understanding the ecology of the rockfish, the visual spectral sensitivities of the dark-adapted rockfishes were measured in the range of visible light (405~660 nm) using a light source of light-emitting diodes (LEDs). In order to assess electrophysiological response of the fish, the ERGs (electroretinograms) of the dark-adapted rockfishes were recorded on a data logger (12 bits) and a laptop computer. Juvenile (n=5; weight: $20.3{\pm}5.2g$; total length: $10.3{\pm}0.7cm$) and adult (n=5; weight: $87.8{\pm}21.8g$; total length: $18.1{\pm}1.3cm$) rockfishes were used in experiment. The visual threshold of juvenile and adult rockfish were 11.66 (log quanta/$cm^2/s$) and 11.81 (log quanta/$cm^2/s$) in 574 nm, respectively. The peak wavelength of the spectral sensitivity in the dark-adapted juvenile and adult rockfish was commonly 551 nm (series of green color). Collectively, these results demonstrate that the rockfish has suitable visual capabilities for inhabiting coastal water in Korea.

• Molecules and Cells
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• 제19권3호
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• pp.382-390
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• 2005

Calcium-binding proteins are thought to play important roles in calcium buffering. The present study investigated the effects of ischemia and reperfusion on calbindin D28K, calretinin, and parvalbumin immunoreactivity in the ganglion cell layer of the rabbit. Rabbits were administered ischemic damage by increasing the intraocular pressure. After 60 and 90 min of ischemia, reperfusion (7 d) was allowed to occur. The b-wave of the electroretinogram (ERG) was reduced by more than 50% and almost 80% in retina given ischemia for 60 and 90 min, respectively. The oscillatory potential (OPs) wave was reduced approximately 50% at 60 min ischemia and 70% at 90 min ischemia. In both normal and ischemic-treated retina, calcium-binding protein immunoreactivity was seen in many cells in the ganglion cell layer. In eyes subjected to 60 min ischemia, there was a decrease of the density of calbindin D28K- (8.29%), calretinin- (14.44%), and parvalbumin- (26.83%) immunoreactive (IR) cells compared to the control retina. In eyes subjected to 90 min ischemia, there was a higher decrease of the density of calbindin D28K- (18.48%), calretinin- (33.59%), and parvalbumin- (54.26%) IR cells than at 60 min. Some calcium-binding protein-IR neurons, especially calretinin-IR neurons, showed aggregations that were abnormally packed together in retina subjected to ischemia for 90 min. The results show that calbindin D28K-, calretinin-, and parvalbumin-IR cells in the ganglion cell layer are susceptible to ischemic damage and reperfusion. The degree of reduction varied among different calcium-binding proteins and ischemic damage times. These results suggest that calbindin D28K-containing neurons are less susceptible to ischemic damage than calretinin- and parvalbumin-containing neurons in the ganglion cell layer of rabbit retina.