• Title/Summary/Keyword: Electrophoretic variation

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Taxonomic Study on Two Subspecies of European Grass Snake (Rhabdophis tigrina) in Korea (한국산 유혈목이 (Rhabodophis Tigrina) 2 아종의 분류학적 연구)

  • 백남극;양서영
    • Animal Systematics, Evolution and Diversity
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    • v.2 no.1
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    • pp.79-92
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    • 1986
  • Electrophoretic method is and morphometric analysis were used to examine genetic and phenetic variation among 16 populations of Rhabdophis tigrina and to clarify the subspecific status of R.t.tigrina and R.t. lateralis in Korea. The degree of genic variation based on 23 presume loci was moderate and was similar to those found for other vertebrates. Interpopulation levels of genic differentiation were slight for all populations studied and overall genetic similarities were =0.970. The number of ventral and subcaudal scales showed clinal variation with increasing tendency in numbers toward south . Discriminant function analysis based on six morphometric characters revealed that there were no morphometric differentiation between two subspecies, therefore it was concluded that they were same taxon and R.t. lateralis (Berthold, 1859) should be treated as synonym of R. t. tigrina(Boie, 1826)

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Evaluation of Genetic Variability in Kenkatha Cattle by Microsatellite Markers

  • Pandey, A.K.;Sharma, Rekha;Singh, Yatender;Prakash, B.;Ahlawat, S.P.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.12
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    • pp.1685-1690
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    • 2006
  • Kenkatha cattle, a draft purpose breed, which can survive in a harsh environment on low quality forage, was explored genetically exploiting FAO-suggested microsatellite markers. The microsatellite genotypes were derived by means of the polymerase chain reaction (PCR) followed by electrophoretic separation in agarose gels. The PCR amplicons were visualized by silver staining. The allelic as well as genotypic frequencies, heterozygosities and gene diversity were estimated using standard techniques. A total of 125 alleles was distinguished by the 21 microsatellite markers investigated. All the microsatellites were highly polymorphic with mean allelic number of 5.95${\pm}$1.9 (ranging from 3-10 per locus). The observed heterozygosity in the population ranged between 0.250 and 0.826 with a mean of 0.540${\pm}$0.171, signifying considerable genetic variation. Bottleneck was examined assuming all three mutation models which showed that the population has not experienced bottleneck in recent past. The population displayed a heterozygote deficit of 21.4%. The study suggests that the breed needs to be conserved by providing purebred animals in the breeding tract.

Systematic and Evolutionary Study on the Genus Parus(Passeriformes : Paridae) in Korea (한국산 박새속(참새목, 박새과) 조류의 계통진화)

  • 박병상;현재범;양서영
    • Animal Systematics, Evolution and Diversity
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    • v.6 no.1
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    • pp.17-28
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    • 1990
  • Electrophoretic methods were used to examine the degree of genic variation and genetic relatedness in 11 populations representing 6 species and subspecies of the genus Parus in Korea. The average degree of genic variation of 3 subspecies of P. major was =1.2, =24.4% , D=0.042, and G=0.058, whereas the rest of the species showed slightly lower degree of genic variation than P. major. Genetic relatedness between subspecies and species in the genus Parus showed similar to those reported at comparable taxnonomic levels in other birds. But it appears to be considerably less than that of non avian taxa. Genetic relatedness between 3 subspecies of P.major and P. varius varius was closely related(=0.80), whereas between P.palustrius hellmayri and P.ater amurensis was relatively remote (=0.67). The presumed divergent times of P.palustrius hellmayri, P, ater amurensis , and P. varius varius were about 1.8, 1.6, and 1.0million years before present respectively, and 3 subspecies of P. major were recently differentiated about 100 thousands years before present.

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Germinability during Overwintering, Field Emergence, and Growth of Shattered Rice Seeds on Paddy Field (논 표면 탈립 벼 종자의 월동중 발아력변화와 월동후 포장 출현 및 생육)

  • 송영주;권영립;오남기;고복래;황창주;박건호
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.1
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    • pp.37-44
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    • 1992
  • Germinability, electrophoretic variation of protein of shattered seeds during overwintering and characteristic of main agronomic traits of off-type rice plant occurring in paddy field harvested by combine were investigated. Germinability of shattered seed decreased as time goes in paddy field in both Japonica and Tongil type varieties. Electrophoretic protein bands become more and more light as time goes. Occurrence of off-type rice plant was higher in Japonica varieties than in Tongil type varieties. Off-type rice plant was shorter in plant height, leaf length, tiller number per hill, internode length, panicle length and spike let number per panicle. Germinability of seeds of off-type rice plant as not significantly different compared to the control variety. Yield of off-type plants of Japonica varieties was on average 5.1kg / 10a and that of Tongil type varieties was on average 0.9kg /10a. Mixing ratio to the control variety was about 0.7% in Japonica varieties and that of Tongil type varieties was about 0.1%.

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Classification of Strains of Ganoderma lucidum (Ganoderma lucidum의 계통분류(系統分類))

  • Shin, Gwan-Chull;Seo, Geon-Sik
    • The Korean Journal of Mycology
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    • v.16 no.4
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    • pp.235-241
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    • 1988
  • This study was carried out to investigate some morphological characters of fruit bodies of Ganoderma lucidum and to classify the fungus on the bases of the genetic character. Some of the isolates of the fungus which originally have the kidney-shaped fruit bodies produced the antler-shaped fruit bodies on artificial media and the latter characters were inherited. Pattern of the pileus surface and thickness of the pileus of the fruit bodies were also considered to be hereditary. Although morphology of the pileus margin and fruiting mode of the fungus were variable among the isolates, they were greatly influenced by environment conditions. Ganoderma lucidum could be classified into two groups and four strains according to the morphology of the fruit bodies on artificial cultivation media. Electrophoretic patterns of esterase, peroxidase, leucine aminopeptidase(LAP) and proteins of fruit bodies and mycelia of Ganoderma lucidum showed high genetic variation. Isozyme patterns of esterase of the mushroom mycelia were applicable for the classification of strains of the fungus. Patterns of proteins, leucine aminopeptidase and peroxidase did not indicate any genetic relation among the fungus strains.

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Allozyme Variation of 6-Phosphogluconate Dehydrogenase in the Freshwater Snail Genus Gyraulus (Pulmonata : Planorbidae)

  • Younghun Jung;Park, Yun-Kyu;Chung, Pyung-Rim
    • The Korean Journal of Malacology
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    • v.16 no.1_2
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    • pp.11-16
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    • 2000
  • The electrophoretic banding patterns of 6-phosphogluconate dehydrogenase (PGD) in the two different chromosomal ploidy groups of Gyraulus were compared. The monomeric or dimeric banding patterns or allelic variations in a locus of PGD were observed in four diploid populations (Osan, Sohre, Kimpo and Kangwha) of G. convexiusculus occurring in Korea, whereas the isozyme banding patterns encoded by at least 3 different loci were shown in the tetraploid populations of G. (Torquis) groups collected from Michigan, the U.S.A. Of 3 different tetraploid groups, G. (T.) circumstriatus group showed 3 monomorphic isozyme banding patterns, and the other 2 populations showed some allelic variations. Such results provided good evidence to differentiate tetraploid subgenus Torquis group from the diploid Gyraulus populations.

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Study on the Effect of the Mg2+ Ion on the Behavior of Anionic Surfactants in Electrophoresis (전기 이동에서 Mg2+ 이온이 음이온 계면활성제의 거동에 미치는 영향)

  • Jeong, Hyuk
    • Analytical Science and Technology
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    • v.18 no.1
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    • pp.35-42
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    • 2005
  • This study reports the effect of $Mg^{2+}$ ions on the behavior of the anionic surfactant in electrophoresis. Intractions of $Mg^{2+}$ ions with carboxylates and sulfonates resulted large changes in their apparent mobilities. A comprehensive analysis of the electroosmotic and electrophoretic mobilities reveals that major cause for the variation of the apparent mobilities of anions arises from the electroosmotic mobilities.

Genetic Variations of Natural and Hatchery Populations of Korean Ayu (Plecoglossus altivelis) by Isozyme Markers

  • Han, Hyon-Sob;Jin, Deuk-Hee;Lee, Jong-Kwan
    • Journal of Aquaculture
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    • v.16 no.2
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    • pp.69-75
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    • 2003
  • Genetic variability and population structure of 11 natural ayu, Plecoglossus altivelis populations and one hatchery stock were assessed by starch gel electrophoretic analysis with 10 enzyme coding loci. Three loci were polymorphic (lower than 0.95 in major allele frequency) in natural populations,2 loci in hatchery stock. The average number of alleles per locus was 1.38. Observed heterozygosities ranged from 0.0235 to 0.088 (0.055 on the average) in natural population while 0.0925 in hatchery stock. The genetic distance among natural populations measured 0.000047-0.005407 and no significant differentiation was observed among them. On the other hand, a signifcant genetic distance was found between natural populations and the hatchery stock with measuring 0.002032-0.O08605. The results in this study suggest that the hatchery stock has diverged from natural populations, and also that careful to maintain sustainable and effective population size (parents number) should be made.

Electrophoretic Study of Semisulcospira gottschei in Korea (한국산(韓國産) Semisulcospira gottschei의 전기영동적(電氣泳動的) 연구(硏究))

  • Kim, Chang Han
    • The Korean Journal of Malacology
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    • v.2 no.1
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    • pp.30-34
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    • 1986
  • Genetic variations of Semisulcospjra gottschei in Korea were investigated by means of starch gel-electrophoresis. The results are as follows; 1) Eight loci of ${\alpha}$-Gpd, Mdh-2, Pept-1, Pgm-2, Gp-1, Gp-2, Gp-3 and Gp-4 were monomorphic, and 8 loci of Mpi, Mdh-1, Pgi, Got, Pept-2, Pept-3, Pgm-1 and Sdh showed genetic variations. 2) Means of allele per locus ($\bar{A}$) and polymorphism (P) were revealed 0.64 and 0.44, respectively. The value of heterozygosity ($H_G$) showed a little more than heterozygosity $H_D$. ($H_D$=0.09, $H_G$=0.13) 3) Genetic variation appears to be due to loci of Got, Pept, Pgi, and Sdh, all of which of the $H_D$ values are high.

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