• The Korean Journal of Ecology
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• 제21권3호
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• pp.301-307
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• 1998

Intraspecific and interspecific isozyme variations and their relationship of 16 local populations in 3 species of Phyllostachys, that is, P. bambusoides, P. nigra var. henonis and P. pubescens were investigated by multi-variate analysis. Leaf isozymes of Phyllostachys such as 6-PGD (6-phosphogluconate dehydrogenase), MDH (malate dehydrogenase), PGI (phosphoglucoisomerase), PRX (peroxidase), PGM (phosphoglutamase), IDH (isocitrate dehydrogenase) showed electrophoretic variations in the number of zymotypes (7, 6, 6, 9, 3 and 5, respectively). In the cluster analysis on the isozymic characteristics, 16 populations were classified into 3 species at the euclid genetic distance of 2.041. P. nigra var. henonis and P. bambusoides were clustered first at 2.813 and then P. pubescens at 3.001. So far, 3 local types of intraspecific ariation were found in P. nigra var. henonis and P. bambusoides.

• 한국잠사곤충학회지
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• 제26권1호
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• pp.35-51
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• 1984

잠품종의 지리적 원산지, 품종, 암·수 별누에 번데기의 체액단백질에 있어서 전기영동적 단백질 패턴의 유사성, 각각의 단백질 밴드의 분포양상, 암·수간의 패턴 차이, 품종별 소유하는 체액단백질 밴드의 종류등에 대해서 잠업시험장에서 보존 중인 129 잠품종을 대상으로 조사한 바, 다음과 같은 결과를 얻었다. 1. 누에번데기 체액단백질의 전기영동 결과 분류된 단백질은 28개 단백질이었고, 각각의 품종이 소유하고 있는 단백질 밴드의 종류는 평균 14 밴드정도였다. 2. 조사된 품종의 지리적 원산지별 품종간의 유사성의 암·수 평균치는 일본종 20.0%, 중국종 28.3%,유럽종 14.3%로 중국종이 유사성이 가장 높다. 3. 전체 조사 집단의 암·수변 유사성은 암:22.5%, 수:23.5%로 거의 비슷한 수준이다. 4. 조사된 129품종의 암·수별 전기영동 패턴의 종류는 암번데기에서 109패턴이 수번데기에서 112패턴이 관찰되었다. 5. 품종내의 암·수별 전기영동 패턴의 차이는 HP3, HP4 단백질 밴드를 제외하면 평균 6.6% 정도이다. 6. 28개 단백질 밴드의 각각의 지리적 원산지별 분포빈도는 원산지에 따라 암·수 공히 차이가 심하다. 7. 모든 품종에 분포두도가 100%인 단백질 밴드는 HP11로서 모든 품종에 공통적으로 존재하는 단백질이었다. 8. 수번데기보다는 암번데기에서 흡광밀도가 높은 HP3, HP4 단백질 밴드가 모든 품종의 암·수에서 뚜렷한 차이를 보였다.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2012년도 춘계학술발표대회
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• pp.107.2-107.2
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• 2012

We used the electrophoretic deposition (EPD) process to fabricate a glass composite and investigated the EPD parameters to find the optimum deposition time by understanding the relationship among the process parameters of zeta potential (ZP), pH, deposition yield and saturation point. A binder and a dispersing agent were mixed properly with glass frit ($0.2{\sim}25{\mu}m$, d50=$8.77{\mu}m$) in an ethyl alcohol medium for the preparation of the slurry. The pH and ZP were in an inverse relationship to each other due to the generation of $H_3O^+$ ions with the addition of the dispersing agent in the slurry. The acidic nature of the dispersing agent was resulted in a decrease of the pH and an increase of the ZP. Otherwise, the pH increased with the addition of the glass frit in the slurry because $H_3O^+$ ions were absorbed on the glass frit. Therefore, the $OH^-$ ions correspondingly increased. The saturation point of EPD was strongly correlated with the variation of the pH in the slurry; this is caused by a chemical reaction between the ethyl alcohol and the ions that make up the glass frit. An adjustment of the pH variation and the saturation point in the slurry can be established with respect to the optimum deposition time in the slurry.

• 한국세라믹학회지
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• 제52권4호
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• pp.284-289
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• 2015

This study examined carbon layer coating on silicon carbide (SiC) fibers by utilizing solid-state and wet chemistry routes to confer toughness to the fiber-reinforced ceramic matrix composites, as an alternative to the conventional pyrolytic carbon (PyC) interphase layer. Electrophoretic deposition (EPD) of carbon black nanoparticles using both AC and DC current sources, and the vacuum infiltration of phenolic resin followed by pyrolysis were tested. Because of the use of a liquid phase, the vacuum infiltration resulted in more uniform and denser carbon coating than the EPD routes with solid carbon black particles. Thereafter, vacuum infiltration with controlled variation in phenolic resin concentration, as well as the iterations of infiltration steps, was improvised to produce a homogeneous carbon coating having a thickness of several hundred nanometers on the SiC fiber. Conclusively, it was demonstrated that the carbon coating on the SiC fiber could be achieved using a simpler method than the conventional chemical vapor deposition technique.

• Bulletin of the Korean Chemical Society
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• 제34권4호
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• pp.1199-1204
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• 2013

Nanocomposites consisting of Sn nanoparticles and graphene oxide (GO) were electrophoretically deposited onto Cu current collectors that was used for anodes in Li ion batteries (LIBs). In order to optimize the electrochemical performance of nanocomposites as an anode material by controlling the oxygen functionality, the GO was subjected to $O_3$ treatment prior to electrophoretic deposition (EPD). During thermal reduction of the GO in the nanocomposites, the Sn nanoparticles were reduced in size, along with the formation of SnO and/or $SnO_2$ at a small fraction, relying on the oxygen functionalities of the GO. The variation in the duration of time for the $O_3$ irradiation resulted in a small change in total oxygen content, but in a significantly different fraction of each functional group in the GO, which influenced the Sn nanoparticle size and the amount of SnO (and/or $SnO_2$). As a result, the EPD films prepared with the GO that possessed the least amount of carboxylic groups (made by treating GO in an $O_3$ environment for 3 h) showed the best performance, when compared with the nanocomposites composed of untreated GO or GO that was $O_3$-treated for a duration of less than 3 h.

• 분석과학
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• 제14권4호
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• pp.356-363
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• 2001

절삭폐수의 여과수 등에 많이 포함된 음이온 계면활성제의 정성 및 정량분석을 위한 기초연구로 SDS를 포함하는 완충용액에서의 음이온 계면활성제들의 거동을 알아보고 최적의 분석조건을 찾기 위한 실험을 수행하였다. SDS 미셀과의 상호작용 결과, 탄소수가 10이상인 카르복실레이트 혹은 술포네이트계 계면활성제는 SDS의 농도가 증가함에 따라, 겉보기 이동도가 크게 변하였다. 겉보기 이동도, 전기삼투 이동도 그리고 전기영동 이동도를 계산하여 비교한 결과, 이러한 겉보기 이동도의 변화는 주로 전기영동 이동도의 변화의 영향임을 알 수 있는 근거를 제시하였다.

• Journal of Plant Biology
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• 제18권4호
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• pp.150-154
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• 1975

Four kinds of Nicotiana species and five varieties belonging to N. tabacum were used as materials for electrophoretic analysis of the tetrazolium oxidase isozyme to examine the taxonomic affinity among them based on the biochemical property. All the five verieties of N. tabacum showed same isozyme bands despite the fact that these varieties had notably varied characteristics including morphological traits. The band patterns were more or less different among the four species. Although N. rustica and N. tabacum were of the same genome group of AABB, their isozyme bands showed considerable difference. N. sylvestris, genome A donor of Nicotiana species, was found to be markedly different from N. tatacum in band pattern, including the absence of system 2 in N. sylvestris.

• 대한화학회지
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• 제18권2호
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• pp.132-137
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• 1974

酸性 mucopolysaccharide는 鯨胎兒의 nucleus pulposus에서 分離하였다. Gel electophoresis에 있어서 酸性 mucopolysaccharide의 分離는 $0.03{\%}$의 hexamine cobaltic chloride 를 含有하는 0.05M 醋酸소-다 緩衝溶液(pH4.8)中에서 가장 좋았다. Spacer gel 上의 mucopolysaccharide 溶液을 $40{\%}$ sucrose 溶液으로 덮었을 때 mucopolysaccharide의 移動度에 變化가 나타나는 것을 觀察하였으며 이 효과는 醋酸소-다 緩衝溶液에 hexamine cobaltic chloride 을 加하면 消失하는 것을 觀察하였다.

• 분석과학
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• 제16권6호
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• pp.504-508
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• 2003

A carbon paste electrode was constructed with peroxidase extracted from Horseradish and the variation of the response of the sensor with pH was investigated. Current profiles showed two highest sensitivities at two pH values respectively. In addition, two bands were observed in the electrophoretic expansion. A coincidence of the two experimental results added support to the possibility that the biosensor has two different isozymes. Assuming that current profiles are the sum of two gaussians, we deconvoluted them and determined the optimum pH of peroxidase isozymes.

• Applied Biological Chemistry
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• 제32권2호
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• pp.116-125
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• 1989

대두로부터 추출, 정제한 Bowman-Birk형 protease inhibitor 들의 함량과 전기영동양상을 비교하고 정제된 inhibitor내에 존재하는 각 isoinhibitor들의 함량을 조사하였으며 종실내 총 chymotrypsin 저해활성도와 cysteine 함량과의 관계를 알아보았다. 8품종의 대두로부터 Sephadex G-75를 이용하여 얻은 정제된 Bowman-Birk형 protease inhibitor들의 함량은 단백질 100g당 $6.67{\sim}9.36g$으로 품종간에 큰 차이가 있었다. 정제된 Bowman-Birk형 protease inhibitor들의 전기영동 양상은 품종간에 많은 차이를 보였으며 각 band의 함량에도 차이가 있었다. 8품종의 정제된 inhibitor에서 나타나는 총 14개의 전기영동 band 중 10개의 band가 protease 저해활성도를 보유하였고 그중 chymotrypsin 저해활성도가 높은 것은 band 7이었으며 그 함량은 단백질 100g당 $0.768{\sim}1.271g$으로 품종간에 차이를 보여 종실내 chymotrypsin 저해활성도가 높은 품종에서 함량이 많았다. 대두품종별 chymotrypsin 및 trypsin저해활성도는 각각 종실 g당 $52,200{\sim}15,225$와 $164,700{\sim}37,500$으로 품종간에 3배 및 4배의 차이를 보였고 단백질 g당으로 환산한 값은 $158,662{\sim}40,033$ 및 500, $608{\sim}120,347$로서 종실 g당 저해활성도와 같은 경향이었으며 3배 이상의 차이를 보였다. 품종별 cysteine 함량은 단백질 g당 $0.1258{\sim}0.0763-mmoles$로서 품종간에 차이를 나타내었으며 이는 실내 총 chymotrypsin 저해활성도와 정의상관관계가 있었다.