• Korean Journal of Veterinary Service
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• v.15 no.1
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• pp.67-80
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• 1992

Avian reoviruses have been implicated in respiratory disease enteric conditions including Cloacal pasting in young thicks, pericarditis, hydropericardium, anaemia with swollen spleen and liver and petechiation of skeletal muscle and viral arthritis. This study was conducted to examine properties of reovirus field 3 strains isolated from affected broiler from several farms. An infectious agent was isolated from leg tendons and intestine of broiler with clinical tenosynovitis. The agent grew well on the chicken embryo kideny cells(CEK). One of them produced cytopathic effects(CPE) of round type and formed intranuclear inclusions, and the other was characterized by CPE of syncytical type and cytoplasmic inclusion. The properties and serological classification of field strains were examined by hemagglutin test, virus neutralization test, agar gel precipitin, electropherotype. They showed no hemagglutination reactions and not well neutralization and to possess common antigens detectable by AGP test. RNA electropherotype presented 10 segment band as the previous report. These data suggest that the field strains and standard strains (1133, 1733) may be the same group.

• The Journal of Korean Society of Virology
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• v.30 no.3
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• pp.189-202
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• 2000

This study was conducted to see what types of bovine rotaviruses were isolated at Jedong farm in Jeju province and Seohwa farm in Chungnum province. The results were as follows. 1. Rotavirus was positively detected in 18 out of 39 fecal samples from calves with diarrhea in Jeju province and in 13 out of 18 fecal samples from calves with diarrhea in Chungnam province. 2. The electropherotype pattern of dsRNA for 31 viruses was shown to be 4 : 2 : 3 : 2 type like traditional group A and the imigration pattern of dsRNA was the long type like NCDV (G6), JBR (G6), B223 (G10) and KK3 (G10). 3. The serotypes of the 18 viruses of Jedong and 9 viruses of Seowha were shown to be group A, subgroup I, G6, and P1 by ELISA and PCR analyses. The serotypes of S-2, S-6, S-9 and S-12 viruses of Seowha were shown to be group A, subgroup I, G10, but was not shown to be P type. 4. The partial nucleotide sequence of VP4 of S-8 was 97% homology with that of BRV 033. VP4 of J-10 showed 96% homology with that of BRV 033 in nucleotide sequence.

• Journal of fish pathology
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• v.6 no.2
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• pp.87-92
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• 1993

In February of 1990, an epizootic disease to masu salmon. Onchorynchus masou cultured at the hatchery of trout in Samchuk. Kwangwondo have broken out and induced heavy mortality. An infectious hematopoietic necrosis virus(IHNV) was isolated from diseased masu salmon fry by the use of fish cell line, CHSE-214. This IHNV isolated from masu salmon was compared with USA isolates of IHNV, SRCV and RB-76 by analysis of virion proteins in sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE) and neutralization tests with two monoclonal antibodies raised against SRCV(MAb SRCV/A4) and RB-76(MAb RB/B5). In the antigenicity and the size of structural proteins. this IHNV, SCS atrain was smilar to RB-76 belonged to the electropherotype I proposed by Hsu et al.(1986).

• Korean Journal of Veterinary Research
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• v.38 no.1
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• pp.65-70
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• 1998

국내분리 소 로타바이러스의 genomic RNA 형태, 분리주간의 교차면역반응 그리고 단클론 항체를 이용한 중화시험에 의해 혈청형을 조사한 바 다음과 같은 결과를 얻었다. 국내분리주들의 genomic RNA 형태는 크게 NCDV 형태(7/11 시료)와 non-NCDV 형태(4/11 시료)의 두가지로 나타났다. 교차면역시험에서 표준주인 NCDV주에 대한 양성혈청은 국내분리주들에 대해서는 비교적 낮은 중화력을 나타내었으나 국내분리주에 대한 양성혈청들은 타 국내분리주 뿐만 아니라 NCDV주에 대해 서로 높은 중화력을 나타내었는데, 분리주 중 678, P44, M4에 대한 양성혈청은 NCDV를 포함한 대부분의 분리주에 대해 100%의 중화력을 나타내었다. 또한 288주에 대한 양성혈청은 288, 678,P44, M4주에 대해서는 높은 중화력을 나타내었으나 다른 분리주들에 대해서는 비교적 낮은 중화력을 나타내었다. 국내분리주의 단크론 항체를 이용한 G혈청형 감별결과는 G6유사형이 45.8%(11/24주), G10 유사형이 54.2%(13/24주)로서 두가지 G형이 존재하였다. 한편 G6형에 반응한 것들의 P형은 표준주인 NCDV주(P1)와는 다른 것으로 확인되었으며, G10 유사형에 속하는 14주는 모두 P11 혈청형으로 판명되었다.