The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 30 Issue 3
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- Pages.189-202
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- 2000
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- 1225-2344(pISSN)
Isolation, Serotyping and Nucleotide Sequence Analysis of Bovine Rotavirus Isolated from Korean Native Cattle
한우에서의 Rotavirus의 분리와 Serotype 결정 및 염기서열 분석
- Yu, Jae-Hyeun (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
- Cha, Kwang-Jong (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
- Kim, Eung-Ryool (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
- Kim, You-Seong (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
- Lee, Young-Kun (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
- Song, Jin-Ook (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
- Cho, Hong-Chan (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
- Ju, Ji-Sun (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
- Park, Bum-Suk (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
- Yoo, Dea-Hwan (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
- Kim, Se-Min (Jedong farm in Jeju) ;
- Ji, Byong-Ju (Seohwa farm in Chungnum) ;
- Lee, Joong-Bok (School of Veterinary Medicine, College of Animal Husbandry, Konkuk University)
- 유제현 (건국대학교 낙농학과) ;
- 차광종 (건국대학교 낙농학과) ;
- 김응률 (건국대학교 낙농학과) ;
- 김유성 (건국대학교 낙농학과) ;
- 이영건 (건국대학교 낙농학과) ;
- 송진욱 (건국대학교 낙농학과) ;
- 조홍찬 (건국대학교 낙농학과) ;
- 주지선 (건국대학교 낙농학과) ;
- 박범석 (건국대학교 낙농학과) ;
- 유대환 (건국대학교 낙농학과) ;
- 김세민 (제동목장) ;
- 지병주 (서화목장) ;
- 이중복 (건국대학교 수의학과)
- Published : 2000.09.30
Abstract
This study was conducted to see what types of bovine rotaviruses were isolated at Jedong farm in Jeju province and Seohwa farm in Chungnum province. The results were as follows. 1. Rotavirus was positively detected in 18 out of 39 fecal samples from calves with diarrhea in Jeju province and in 13 out of 18 fecal samples from calves with diarrhea in Chungnam province. 2. The electropherotype pattern of dsRNA for 31 viruses was shown to be 4 : 2 : 3 : 2 type like traditional group A and the imigration pattern of dsRNA was the long type like NCDV (G6), JBR (G6), B223 (G10) and KK3 (G10). 3. The serotypes of the 18 viruses of Jedong and 9 viruses of Seowha were shown to be group A, subgroup I, G6, and P1 by ELISA and PCR analyses. The serotypes of S-2, S-6, S-9 and S-12 viruses of Seowha were shown to be group A, subgroup I, G10, but was not shown to be P type. 4. The partial nucleotide sequence of VP4 of S-8 was 97% homology with that of BRV 033. VP4 of J-10 showed 96% homology with that of BRV 033 in nucleotide sequence.