• Applied Microscopy
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• v.28 no.4
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• pp.503-512
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• 1998

Fine structure of the cutaneous pigments in the black widow spider, Latrodectus mactans are studied with light and electron microscopes. The cutaneous pigments are only observed in epidermal layer just beneath the cuticle. These pigments are compactly distributed around the spinnerets which located at caudal area of the abdomen. According to the fine structural characteristics of the pigment granules, two main types of guanine pigment granules-carotenoid vesicles and reflecting platelets - are observed in the cytoplasm of the epidermal cells. Morphological features of these pigment granules are characterized as the electron dense carotenoid vesicles and the electron lucent reflecting platelets. Marginal electron density of the carotenoid vesicle is different from that of internal region, whereas the reflecting platelets have laminated crystalline granules. Typiral structures of these pigment granules are very similar to those of invertebrate's chromatophores, especially erythrophores and iridophores. Moreover differentiation of these pigment granules are also originated from the small vesicles of Golgi complexes similarly to those of cutaneous chromatophores.

• Applied Microscopy
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• v.50
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• pp.13.1-13.7
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• 2020

We examined the morphology of fertilized egg and ultrastructures of fertilized egg envelopes of Ancistrus cirrhosus belong to Loricariidae using light and electron microscopes. The fertilized eggs formed a mass on the spawning place and were yellowish, spherical, non-transparent, demersal, adhesive, and a narrow perivitelline space. But, the adhesiveness of fertilized eggs was disappeared after spawning excluding contact parts. The micropyle with funnel shape was surrounded by 15-19 furrow lines of egg envelope in a spoke-like pattern. The outer surface of egg envelope has smooth side and inner surface of egg envelope was rough with grooves. Also, the total thickness of the fertilized egg envelope was about 32.58 ± 0.85 ㎛ (n = 20), and the fertilized egg envelope consisted of three layers, an outer adhesive electron-dense layer, a middle layer with low electron density and an inner electron-dense layer with grooves in counter structure from other most teleost. Collectively, these morphological characteristics and adhesive property of fertilized egg, and ultrastructures of micropyle, outer surface, and section of fertilized egg envelope are showed species specificity.

• Applied Microscopy
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• v.2 no.1
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• pp.39-46
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• 1972

There are many kinds of microscopes suitable for general studies; optical microscopes(OM), conventional transmission electron microscopes (TEM), and scanning electron microscopes(SEM). The optical microscopes and the conventional transmission electron microscopes are very familiar. The images of these microscopes are directly formed on an image plane with one or more image forming lenses. On the other hand, the image of the scanning electron microscope is formed on a fluorescent screen of a cathode ray tube using a scanning system similar to television technique. In this paper, the features and some applications of the scanning electron microscope will be discussed briefly. The recently available scanning electron microscope, combining a resolution of about $200{\AA}$ with great depth of field, is favorable when compared to the replica technique. It avoids the problem of specimen damage and the introduction of artifacts. In addition, it permits the examination of many samples that can not be replicated, and provides a broader range of information. The scanning electron microscope has found application in diverse fields of study including biology, chemistry, materials science, semiconductor technology, and many others. In scanning electron microscopy, the secondary electron method. the backscattererd electron method, and the electromotive force method are most widely used, and the transmitted electron method will become more useful. Change-over of magnification can be easily done by controlling the scanning width of the electron probe. It is possible. to continuously vary the magnification over the range from 100 times to 1.00,000 times without readjustment of focusing. Conclusion: With the development of a scanning. electron microscope, it is now possible to observe almost all-information produced through interactions between substances and electrons in the form of image. When the probe is properly focused on the specimen, changing magnification of specimen orientation does not require any change in focus. This is quite different from the conventional transmission electron microscope. It is worthwhile to note that the typical probe currents of $10^{-10}$ to $10^{-12}\;{\AA}$ are for below the $10^{-5}$ to $10^{-7}\;{\AA}$ of a conventional. transmission microscope. This reduces specimen contamination and specimen damage due to heatings. Outstanding features of the scanning electron microscope include the 'stereoscopic observation of a bulky or fiber specimen in high resolution' and 'observation of potential distribution and electromotive force in semiconductor devices'.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2007.11a
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• pp.461-462
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• 2007

• Journal of the Korean Ceramic Society
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• v.36 no.10
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• pp.1132-1138
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• 1999

• Proceedings of the Korea Information Processing Society Conference
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• 2009.11a
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• pp.395-396
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• 2009

Electron tomography를 이용한 3차원적 영상 시각화는 electron microscopy를 통해 하나의 실험 대상으로부터 연속된 이미지를 생산함으로써 이루어진다. 이미지 데이터 내부에는 대용량의 정보값을 포함하고 있어 3차원 구조물로의 변환이 가능하다. electron tomography 작업 과정 중 고해상도 원본 이미지에 pattern recognition 알고리즘이 적용된 필터링을 적용하면 실험에 필요한 데이터의 정보 손실을 최소화한 상태에서 electron tomography 시스템의 효율성을 높일 수 있다. 또한 tomographic econstruction이 진행되는 각 단계에 hanning windowing을 적용하면 불필요한 정보 값이나 노이즈 등을 효과적으로 제거할 수 있다. 윤곽선 데이터의 효과적 활용을 위하여 sobel 필터 처리를 할 경우 관찰하고자 하는 대상의 윤곽선 특징을 뚜렷하게 시각화 할 수 있었다. 본 연구를 통하여 데이터의 시각화 과정에서 실험의 신뢰성 확보를 위해 원본 이미지를 기반으로 하는 tomogram과 필터링을 적용한 tomogram을 비교하여 최종 결과물의 정확도를 높이고, electron tomography를 통한 결과물의 질적 향상을 유도할 수 있음을 확인하였다.

• 한국전자현미경학회:학술대회논문집
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• 2005.11a
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• pp.150-152
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• 2005

• Applied Microscopy
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• v.42 no.2
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• pp.105-109
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• 2012

Electron tomography (ET) of biological specimens is performed from a series of images obtained over a range of tilt angles in a transmission electron microscope. When using the high voltage electron microscope (HVEM), various noises appear in EM images acquired from thick sections by high voltage electron beam. In order to obtain an adequate result in electron tomograms that allow visualization of rather complex and mega-cellular structure such as brain tissue, it is necessary to remove the noise in each original tilt images of thick section. Using band-pass filtering of original tilt images, the filtered images are obtained and used to assemble a reconstructed tomogram. The qualified 3D tomogram from filtered images results in a considerable reduction of the noises compared to conventional tomogram. In conclusion, this study suggests that band-pass filtering is effective to improve the brightness and intensity of HVEM produced tomograms acquired from micron-thick sections of biological specimens.

• Journal of the Korea Convergence Society
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• v.8 no.8
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• pp.197-202
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• 2017

Three-dimensional melanin granules of mummy's hair analyzed using high voltage electron microscopy at 1250kV. The melanin granules in the cortex of mummy's hair located in close proximity to the outer of cortex in the adjacent place of the cuticles. A lot of melanin granule performed in this area. It is a distinguished difference from modern human. While it rotated up to 60 degree counter-clockwise and taken a photo per one degree using high voltage electron microscopy. The melanin granule displayed with long elliptical and variable at the size. The size of granule is measured from $0.3{\sim}0.6{\mu}m$ in minimum diameter and $0.5{\sim}1{\mu}m$ in maximum diameter. Conclusionally, high voltage electron microscope has higher resolution and penetration power than conventional transmission electron microscope that could be load biological thick specimen.

• Applied Microscopy
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• v.42 no.1
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• pp.49-52
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• 2012

For TEM study of biological samples or polymers that are contained in organic structure, it is often required that the sample is prepared by using ultramicrotome and stained with proper agents to increase the contrast of organic structure. In this study, we investigated an efficient TEM sample preparation method for ductile heterogeneity material by using ultramicrotomy. Cryo-ultramicrotomy is a suitable method that is capable of rendering sample hardness for various ductile materials. However, it has several factors to consider, such as experimental cost, working time and finding the optimal staining conditions. To satisfy these considerations, we prepared TEM sample by using ultramicrotome without cryofunction, and secured the sample hardness by applying the staining process prior to ultrathin sectioning. The cross-linked polyethylene structure in the sample was stained with the 2% $RuO_4$ solution in a sealed test tube for 24 hours at $4^{\circ}C$. After the sample staining, ultrathin sections of sample were prepared using ultramicrotome. As a result, it was revealed that the difficulties associated with staining of ultrathin sections prepared by low-temperature conditions were improved. In addition, appropriate staining depth of sample could be selected for sectioning process. The quality of TEM sample obtained by using this method was better than that of cryo-ultramicroscopy. Finally, it is expected that our method could be effectively applied in TEM sample preparation for a variety of nano-bio convergence materials.