• Asian-Australasian Journal of Animal Sciences
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• v.30 no.6
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• pp.781-787
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• 2017

Objective: The early growth response (Egr) family consists of four members (Egr1, Egr2, Egr3, and Egr4) that are zinc finger transcription factors. Among them, Egr3 is involved in transcriptional regulation of target genes during muscle spindle formation and neurite outgrowth. We previously showed that the immunoreactive Egr3 is localized on oocyte spindle and accumulate near the microtubule organizing center during meiosis I in mice. Egr3 was also shown to be localized on spermatocytes. We herein investigated if Egr3 is expressed in mouse gonads and if Egr3 blockade results in any defect in oocyte maturation. Methods: Expression of Egr3 in mouse gonads was examined by reverse transcription-polymerase chain reaction. Full-length Egr3 and truncated Egr3 (${\Delta}Egr3$) complementary RNAs (cRNAs) with Xpress tag at N-terminus and DsRed2 at C-terminus, and small interfering RNA (siRNA) targeting Egr3 were microinjected into mouse oocytes at germinal vesicle stage. Localization of microinjected Egr3 was examined by confocal live imaging and immunofluorescence staining. Results: Egr3 mRNA was detected in mouse ovaries and testes from 1 to 4 week-old mice. An uncharacterized longer transcript containing 5'untranslated region was also detected in 3 and 4 week-old gonads. Microinjected Xpress-Egr3-DsRed2 or Xpress-${\Delta}Egr3$-DsRed2 localized to nuclei and chromosomes during meiotic progression. Microinjection of these cRNAs or Egr3 siRNA in oocytes did not affect meiotic maturation. Immunofluorescence staining of Egr3 in Xpress-${\Delta}Egr3$-DsRed2-injected oocytes showed a positive signal only on meiotic spindle, suggesting that this antibody does not detect endogenous or exogenous Egr3 in mouse oocytes. Conclusion: The results show that Egr3 localizes to chromosomes during meiotic progression and that certain antibodies may not faithfully represent localization of target proteins in oocytes. Egr3 seems to be dispensable during oocyte maturation in mice.

• Journal of Energy Engineering
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• v.11 no.3
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• pp.203-209
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• 2002

In this study the characteristics of E-EGR valve developed by Unick were analyzed and the feasibility of application to vehicles were evaluated. Smart car (3$\ell$/100 km) and engine which is small-displacement size, 0.8-liter, of diesel passenger car developed from Mercedes-Benz were used for this experiment. It was installed a 3-cylinder turbo-charged light duty diesel engine with an electronic EGR valve. After the analysis and comparison of E-EGR valve performance under test benches, the estimation of vehicle application was executed through the EGR map and CVS-75 test result measured on the chassis dynamometer.

• Journal of the Korean Society of Combustion
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• v.19 no.3
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• pp.26-33
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• 2014

We examined the characteristics of $NO_x$ emission for CH4/air non-premixed flames using the exhaust gas recirculation(EGR) methods, which are the air-induced EGR(AI-EGR) and fuel-induced EGR(FI-EGR) methods. Our experimental results show that the $NO_x$ emission index($EI_{NOx}$) decreased with increasing EGR ratio. In the range needed to form a stable flame, the reduction rate of $EI_{NOx}$ for the FI-EGR method was approximately 29% when the EGR ratio was 20%, and the reduction rate for the AI-EGR method was approximately 28% with 25% of the EGR ratio. According to the flame structure based on numerical results, high temperature regions for the FI-EGR method were narrower and lower than those for the AI-EGR method at the same EGR ratio. Furthermore, based on the experimental results for swirl flames, the reduction rate of $EI_{NOx}$ for the FI-EGR method was approximately 49% with 15% of the EGR ratio, while the maximum reduction rate for AI-EGR method was approximately 45% with 25% of the EGR ratio. Consequently, we verified that the FI-EGR method was more effective than the AI-EGR method in reducing $NO_x$ emission for non-premixed flames with EGR. We expect that the results of this study will provide fundamental information relating to hybrid combustion systems, which can be used in the design of combustion systems in the future.

• Journal of Advanced Marine Engineering and Technology
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• v.35 no.4
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• pp.379-387
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• 2011

Research and development of LP EGR system for the performance improvement and emission reduction on diesel engine is proceeding at a good pace. LP EGR system seems to be helpful method to further reduce$NO_x$ emissions while maintaining PM emissions at a low level because the boost pressure is unchanged while varying EGR rate. This study is experimentally conducted on a 2.0L common rail DI engine at the medium load condition (2000 rpm, BMEP 1.0 MPa, boost pressure 181.3 kPa) that difficult to use large amount of EGR gas because of deteriorations of performance and fuel consumption. And we investigated the characteristics of performance and fuel consumption while varying EGR systems. The overall results using LP EGR system equipped with ETC identified benefits on reduction of PM and improvement of fuel consumption and thermal efficiency while keep the $NO_x$ level compared to HP EGR and LP EGR with back pressure valve.

• Proceedings of the KSME Conference
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• 2001.11b
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• pp.928-933
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• 2001

EGR(Exhaust Gas Recirculation) is an effective strategy to control nitrogen oxides emissions from diesel engine. The EGR reduces $NO_x$ through lowering the oxygen concentration in the combustion chamber as well as through heat absorption. However, application of EGR system is difficult because of the penalty in fuel consumption and the increase in particulate matter. The engine used for the experimental was a 3-cylinder 0.8-liter turbo-charged light duty diesel engine with an electronic EGR valve. In this study, experiments were performed at variable vehicle speeds and loads on the chassis dynamometer. To evaluate the exhaust emissions with the EGR system testing was performed using cvs-75 mode test procedure. Results of the cvs-75 mode test achieve sufficiently to meet EURO3 standards.

• Transactions of the Korean Society of Automotive Engineers
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• v.15 no.2
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• pp.65-73
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• 2007

The use of an Exhaust Gas Recirculation(EGR) for a diesel engine with variable geometry turbocharger(VGT) has confronted how to obtain the amount of EGR for NOx reduction requirement at wide operating range and less side effect. Through a combined effort of modeling(wave action simulation) and experiment, an investigation into the effect of EGR area ratio and pipe length on EGR characteristics of common rail diesel engine with VGT has been performed. For accurate computation, calibration of constants involved in empirical and semi-empirical correlations has been performed at a specific operating point, before of its use for engine simulation. From the results of this study, it was found that EGR rate is sharply increased with increasing EGR area ratio until area ratio of 0.3. However, the effect of EGR area ratio on EGR rate is negligible beyond this criteria. This study also investigates the effect of EGR pipe length on a EGR amount and pulsating flow characteristics at EGR junction. The results showed that the longer EGR pipe length, the lower EGR amount was achieved due to the flow loss resulting in lower amplitude of pressure wave.

• Korean Journal of Biological Psychiatry
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• v.29 no.2
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• pp.33-39
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• 2022

Objectives The early growth response 3 (EGR3) gene located in chromosome 8p21.3 is one of the susceptibility loci in many psychiatric disorders. EGR3 gene plays critical roles in signal transduction in the brain, which is involved in neuronal plasticity, neuronal development, learning, memory, and circadian rhythms. Recent studies have suggested EGR3 as a potential susceptibility gene for bipolar disorder (BPD). However, this requires further replication with an independent sample set. Methods To investigate the genetic role of EGR3 in Korean patients, we genotyped six single-nucleotide polymorphisms (SNPs) in the chromosome region of EGR3 in 1076 Korean BPD patients and 773 healthy control subjects. Results Among the six examined SNPs of EGR3 (rs17088531, rs1996147, rs3750192, rs35201266, rs7009708, rs1008949), SNP rs35201266, rs7009708, rs1008949 showed a significant association with BPD (p = 0.0041 for rs35201266 and BPD2, p = 0.0074 for rs1008949 and BPD, p = 0.0052 for rs1008949 and BPD1), which withstand multiple testing correction. In addition, the 'G-C-C-C' and 'G-C-G-C' haplotypes of EGR3 were overrepresented in the patients with BPD (p = 0.0055, < 0.0001, respectively) and the 'G-T-G-C' haplotype of EGR3 was underrepresented in patients with BPD (p = 0.0040). Conclusions In summary, our study supports the association of EGR3 with BPD in Korean population sample, and EGR3 could be suggested as a compelling susceptibility gene in BPD.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.10
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• pp.879-886
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• 2013

This study aims to provide helpful suggestions for understanding the effect of high EGR on DME HCCI combustion. This study determined which between oxygen partial pressure and oxygen concentration was the main factor affecting the LTHR heating ratio. Furthermore, EGR and the supercharging effect were investigated. To define the parameters for the EGR ratio and supercharging pressure, a numerical analysis of the chemical reaction was conducted under the following conditions: (1) variation of EGR ratio, oxygen concentration, and oxygen content; (2) variation of oxygen partial pressure while the oxygen concentration was almost constant; and (3) variation of oxygen concentration while oxygen partial pressure was constant with EGR and supercharging. The results show that an increase in EGR reduces the combustion duration. On the other hand, an increase in boost pressure increases the combustion duration. Finally, the EGR and boost pressure affect the amount of increase in LTHR.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.3
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• pp.291-298
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• 2010

A conventional EGR cooler, which is used in an EGR system of an automobile diesel engine, has a low heat-exchange efficiency. To maximize the heat transfer between the exhaust gas and coolant, dimples are formed on the surface of heat exchange tubes. When designing the dimpled EGR cooler, the net heat transfer areas in the conventional and dimpled tube-type EGR coolers are compared. Structural integrity evaluations are also performed by combining finite element analysis with a homogenization method. Subsequently, the process of manufacturing the dimpled tube, i.e., the formation of dimples, edge bending, center v-notch bending, compression, and plasma welding, is established and carried out. Thus, the dimpled EGR cooler is developed, and its performance is verified.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.11
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• pp.4137-4144
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• 2010

In this study, the simulations were carried out to show the effect of the EGR configuration in a passenger diesel engine with 2-stage turbocharger on the EGR rate. The AMESim and IFP Engine Library were used to make the program for the simulation. Three EGR configurations, HPL(high pressure loop), LPL(low pressure loop), and SLPL(semi low pressure loop), were considered. The EGR rate in the HPL and LPL EGR routes were 6.4% and 10.0% respectively but the rate in SLPL route was 18.0% and their air/fuel ratio for all three cases was 21. Therefore the SLPL EGR configuration may be positively considered in the design of the passenger diesel engine with 2-stage turbocharger.