• Title/Summary/Keyword: Eggs

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Prevention of the Green-grey Discoloration in Retorted Liquid Whole Eggs (가압살균한 전난액의 녹색변이 방지에 관한 연구)

  • 송인상;유익종;강통삼;민병용
    • Korean Journal of Poultry Science
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    • v.11 no.1
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    • pp.27-32
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    • 1984
  • In order to use the liquid whole eggs as an ingredients of retorted products, the provention of the green-grey discoloration of retorted liquid whole eggs by the addition of the Na$_2$EDTA was investigated. And palatability change of the retorted liquid whole eggs by the addition of Na$_2$EDTA was also investigated. The results obtained were summarized as follows: 1. More severe green-grey discoloration was occurred when higher cooking temperature and longer cooking time were used. 2. Green-grey discoloration of retorted liquid whole eggs could be prevented by the addition of about 0.015% of Na$_2$EDTA. 3. The palatability of retorted liquid whole eggs was not lowered by the addition of 0.02% of Na$_2$EDTA and green grey discoloration was not developed during the storage time of 22 days at room temperature.

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Intra- and Inter-plant Distribution of Helicoverpa assulta (Lepioprera: Noctuidae) eggs in Red Pepper and Tobacco Fields (고추와 담배포장에서의 담배나방 알의 공간분포 및 기주식물내 분포)

  • 한만위;이준호;손준수
    • Korean journal of applied entomology
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    • v.33 no.1
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    • pp.6-11
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    • 1994
  • The Spatial distribution pattems of the oriental tobacco budworm (OTB), Helicoverpa assuita, eggs w were studied in red pepper and tobacco fields. With a plant as a sample Unit. Taylor's power law analyses indicated that om egg spatial patterns were clumped in red pepper fields (a=1.3914, b = 1.1648) and were unifom in tobacco fields (a = 1.6035, b = 0.6880). In red peppers om eggs w were found in leaves (76.2%), fruits (16.8%), stems (6.5%), and flowers (0.4%). The upper leaf surface ( (70.1 %) contained sianificantly more eggs than the lower surface (25.2%). In tobaccos most eggs were also found in leaves. However, the lower surface (66.3%) contained significantly more eggs t th

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Induction of Non-Diapause Eggs and Manifestation of Quantitative Characters by Low Temperature Incubation of Eggs in the Silkworm, Bombyx mori L.

  • Singh, Ravindra;Jayaswal, K.P.;Rao, D.Raghavedra;Kariappa, B.K.;Premalatha, V.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.4 no.2
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    • pp.149-153
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    • 2002
  • Induction of non-diapause eggs and manifestation of quantitative characters were studied in different seasons by low temperature incubation of eggs of a low yielding diapause with coloured cocoons silkworm breed, RDI of Bombyx mori. Hundred percent of nandiapause eggs were induced when the egg incubation was carried out at 15$^{\circ}C$ followed by incubation initially at $25^{\circ}C$ and then at $15^{\circ}C$. The diapause-nondia-pause relation was found to be reversible. Analysis of variance study demonstrated significant variation in all the quantitative characters whereas significant variation due to different incubation treatments was observed for larval span, fifth instar larval span, cocoon yield/10,000 larvae by weight, cocoon weight, cocoon shell weight, and cocoon shell ratio. study on hybrid vigour skewed significant heterosis over mid parent value for four economic characters viz., cocoon weight, cocoon shell weight, cocoon shell ratio and filament length in a Fl hybrid between RD1 with diapause eggs and Japanese type bivoltine $NB_{4} D_{2}$.

Changes of Fluorescent Substances and Free Amino Acids in Formed Eggs of the Silkworm(Bombyx mori) (가잠조성난의 형광물질과 유리아미노산의 변화)

  • 성수일;박광의;김주읍
    • Journal of Sericultural and Entomological Science
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    • v.20 no.1
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    • pp.11-14
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    • 1978
  • This experiment was attempted to investigate the effect of removal of suboesophageal ganglion of Bombyx mori on the changes of free amino acids and fluorescent substances by means of paper chromatography. 1. There are no differences of quantity and component of free amino acids of formed eggs in moths between treatment and control. 2. Riboflavine content of formed eggs in treated moths is less than that of formed eggs in untreated moths. 3. Fluorescent substance which appeared on Rf value 0.06 was not detected in formed eggs of treated moths but found in those of control. 4. Fluorescent substance which appeared on Rf value 0 was noted in formed eggs of treated moths but not in those of control.

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Molecular Identification and Bimonthly Abundance of Fish Eggs Collected in the Coastal Waters of Sagye, Jejudo Island (제주도 사계연안 어란의 분자동정과 격월별 출현양상)

  • Han, Song-Hun;Kim, Maeng Jin;Kim, Joon Sang;Song, Choon Bok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.6
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    • pp.829-836
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    • 2017
  • This study investigated the species composition and abundance of floating fish eggs to determine the timing and location of spawning of fish inhabiting the coastal waters of Sagye, Jejudo Island. Eggs were collected with a Bongo net bimonthly from May 2009 to February 2010. Identifications were based on nucleotide sequences of the mitochondrial cytochrome c oxidase subunit I (COI) gene. Eggs were determined to belong to 43 distinct taxa, 35 of which were identified to the species level. The assemblage spanned eight orders, 23 families, and 32 genera. The number of taxa collected varied from month to month, with 14 taxa (12 species) found in June 2009, 11 taxa (10 species) in October 2009, 10 taxa (nine species) each in August 2009 and February 2010, eight taxa (six species) in April 2009, and five taxa (four species) in December 2009. Five abundant species (Branchiostegus japonicus, Engraulis japonicus, Pseudolabrus sieboldi, Goniistius zonatus, and Halichoeres tenuispinis) together represented 52.8% of the total number of eggs collected during the study.

Quantification of Reproductive Output of the Butter Clam, Saxidomus purpuratus(Sowerby, 1852) Using Enzyme-Linked Immunosorbent Assay (ELISA)

  • Park, Kyung-Il;Choi, Jin-Woo;Choi, Kwang-Sik
    • Ocean and Polar Research
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    • v.25 no.3
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    • pp.249-256
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    • 2003
  • An immunological method was developed in this study to quantify reproductive output of the female butter clam, Saxidomus purpuratus. A clam egg-specific polyclonal antibody was developed using the purified butter clam egg as an antigen. An indirect Enzyme-Linked Immunosorbent Assay (ELISA) was used in quantitative measurement of the eggs. Size of the butter clam eggs ranged from $70.81{\pm}7.52{\mu}m$ in histology or $88.56{\pm}11.31{\mu}m$ in intact eggs. The predominant egg constituent was protein (37.44%), followed by lipids (11.40%) and carbohydrates (9.68%). The SDS-PAGE showed that the egg proteins are composed of several peptides of molecular weights consisting of 247, 200, 99, 91, 54 and 47 kDa. ELISA indicated that the clams collected from Geoje Island in May 2002 produced 8.2 to 26.8% of their body weight as eggs or 9,307,309 to 31,156,333 with a mean of 16,931,893 eggs per individual clam. The results of this study thus suggest that indirect ELISA using rabbit anti-clam egg IgG as a primary antibody is a rapid, affordable and sensitive method to assess reproductive output of 5. purpuratus and possibly other bivalves using a small amount of eggs.

Effects of Nonylphenol on Early Development of Olive Flounder, Paralichthys olivaceus (Nonylphenol이 넙치, Paralichthys olivaceus의 초기 발생에 미치는 영향)

  • 문순주;김진완;나오수;김병호;이영돈;김형배;최영찬
    • Environmental Analysis Health and Toxicology
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    • v.17 no.1
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    • pp.1-6
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    • 2002
  • Effects on nonylphenol (NP) treatment on early development of fertilized eggs and survival of larvae in olive flounder, Paralichthys olivaceus were investigated. Fertilized eggs and hatched larvae were exposed to aqueous solutions of NP at nominal concentrations of 50, 100, 150 and 200 $\mu\textrm{g}$/L. In the control I and II (methanol-carrier control) groups, the hatchability of fertilized eggs were 90.7$\pm$7.02 and 90.0$\pm$5.29% (P <0.05), respectively. However, treatments of NP concentrations at 50, 100, 150 and 200 ug/L were reduced to 78.7$\pm$4.16, 46.0$\pm$9.17, 48.0$\pm$3.46 and 33.3$\pm$11.02% (P<0.05), respectively. The time to hatching of fertilized eggs was delayed in high-dose NP treatment groups rather than control groups. Fertilized eggs of NP immersed group could not hatch normally, and also vertebra of the larvae observed as abnormal shape. Cumulative mortality of hatched larvae after 26hr NP treatment was 38.3, 78.3, 88.3 and 100% in NP 50, 100, 150 and 200 ug/L treatment groups, respectively. Whereas, control I and II were 11.7, 16.7% (P<0.05). Hatched larvae showed most death at the embryonic development stage with abnormalities. These results suggest that NP treatment in fertilized eggs and larvae was inhibited as normally development for fertilized egg and growing of larvae.

Studies on the Characteristics of Anti-Zona Antibody II. Effect of Anti-Zona Antibody on Fertilization of Porcine and Mouse Eggs In Vitro (항투명대 항체의 특성에 관한 연구 II. 항투명대 항체가 돼지난자와 생쥐난자의 체외수정에 미치는 영향)

  • 김은영;박세필;정형민;정길생;김종배
    • Korean Journal of Animal Reproduction
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    • v.14 no.2
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    • pp.115-124
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    • 1990
  • These experiments were undertaken as a basic study to develop immunocontraceptive vaccine and to understand the role of zona pellucidae in early fertilization process by investigating the effect of monoclonal and polyclonal antibody to porcine zona pellucidae and polyclonal antibody to mouse zona pellucidae on the fertilization of porcine and mouse eggs in vitro. The results obtained in these experiments were summarized as follows : 1. Treatment of porcine and mouse eggs with undiluted anti-zona serum produced intense precipitation layer on the poricne and mouse zonae, respectively, thus resulting in the total inhibition of sperm adherence on surface of zona. 2. In vitro fertilization of eggs pre-treated with 0.3∼10% of various antibodies was examined, and resulting in that 5 and 10% of rabbit polyclonal antibodies to porcine zona inhibited completely both in vitro fertilization and polyspermy of porcine eggs while monoclonal to porcine zona and rabbit polyclonal antibody to mouse zona did not inhibit in vitro fertilization but monoclonal antibody reduced the rate of polyspermy compared to that of control group. Almost the same results were obtained in the study on the effect of anti-zona serum on in vitro fertilization of mouse eggs.

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Quality Inspection and Sorting in Eggs by Machine Vision

  • Cho, Han-Keun;Yang Kwon
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1996.06c
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    • pp.834-841
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    • 1996
  • Egg production in Korea is becoming automated with a large scale farm. Although many operations in egg production have been and cracks are regraded as a critical problem. A computer vision system was built to generate images of a single , stationary egg. This system includes a CCD camera, a frame grabber board, a personal computer (IBM PC AT 486) and an incandescent back lighting system. Image processing algorithms were developed to inspect egg shell and to sort eggs. Those values of both gray level and area of dark spots in the egg image were used as criteria to detect holes in egg and those values of both area and roundness of dark spots in the egg and those values of both area and roundness of dark spots in the egg image were used to detect cracks in egg. Fro a sample of 300 eggs. this system was able to correctly analyze an egg for the presence of a defect 97.5% of the time. The weights of eggs were found to be linear to both the projected area and the perimeter of eggs v ewed from above. Those two values were used as criteria to sort eggs. Accuracy in grading was found to be 96.7% as compared with results from weight by electronic scale.

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Teratological Effects of Enrofloxacin on the Embryos and Fetus During the Hatching of Chicken Eggs (병아리 태자에 대한 엔로플록사신의 최기형 독성 연구)

  • Lee, Jin-Heon;Lee, Ji-Hee;Cho, Y-Eaun
    • Journal of Environmental Health Sciences
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    • v.34 no.4
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    • pp.292-299
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    • 2008
  • The purpose of this study was to investigate the teratological effects of enrofloxacin, a veterinary antibiotic, on the embryos and fetus of hatching chicken eggs. A control group and four experimental fertilized egg groups were set up. The four experimental groups were injected with 0.05 ml, 0.1 ml, 0.2 ml, and 0.3 ml enrofloxacin ($50{\mu}g/ml$) respectively. During the hatching period, the weights of total eggs, inside material(yolk and white) and embryos or fetuses, embryo growth, and teratological effects were investigated. In the experimental groups (fertilized eggs injected with 0.05 ml, 0.1 ml, 0.2 ml and 0.3 ml enrofloxacin), the weights of total eggs were decreased, but the yolk and white weights were increased by the higher amount of antibiotics. The weights of embryos (fetuses) in experimental groups were 86.1%, 78.6%, 65.6% and 61.4% of the control, respectively. Retarded growth, deformity and embryo loss were observed in experimental groups. Teratological effects such as undeveloped eyes, wings and legs, and deformed head and bill were also detected. In conclusion, we found that veterinary antibiotics, enrofloxacin have made teratological effects on the embryos and fetus of hatching eggs.