• Title/Summary/Keyword: Egg parasitoid

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Survey the Occurrences and Establishment of Environment-friendly Control System of Ricania shanthungensis in Jeonnam Province (전남지역 갈색날개매미충 발생현황과 친환경 방제)

  • Choi, Duck-Soo;Ma, Kyung-Cheol;Kim, Hyo-Jeong;Lee, Jin-Hee;Oh, Sang-A;Kim, Seon-Gon
    • Korean Journal of Organic Agriculture
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    • v.26 no.3
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    • pp.439-452
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    • 2018
  • This study was carried out to establish of environment-friendly control system and to survey the occurrence status of Ricania shanthungensis in Jeonnam province from 2016 to 2017. R. shanthungensis occurred at 1,344 ha in 61 towns of 15 cities in Gwangju and Jeonnam province. At four sites in the stationary area, the densities of eggmass and pupae were lower in 2017 than in 2016, the hatching rate decreased, and the first hatching time, emergence of adults, and the start of spawning tended to be slower. Telenomus sp., an egg parasitoid of R. shanthungensis, was found in most areas. Sunchon had the highest rate of 46.1%, followed by Muan 40.6, Goksung 29.2, Gurye 25.8, and Gwangju 17, respectively. The optimum spraying time for the control of R. shanthungensis was early March. The spraying material and the dilution multiples are 10 times of the machine oil at environmental friendly cultivation and 500 times of the chlorpriphosphate wp. at normal cultivation. When sprayed these materials, 95% of egg prohibited to hatch. There was more than 80% of the insecticidal effects on the organic materials of the machine oil emulsion, the Sophora root extrect + microbial extract agent, and the castor emulsion. For adults, Sophora root extrect + microbial extract, neem extract, and machine oil were better. Four kinds of chemical pesticides such as dinotefuran wp were effective for nymph and adult control. We have developed an adult catching device using the most preferred daylight color and behavioral habits of R. shanthungensis. The capture device consisted of two daylight compact lamps (30W and 20W), a yellow plate, and a catcher using water, and caught about 700 individuals a day. Based on the above results, we have established a system for controlling and life cycle of R. shanthungensis in Jeonnam province.

Ecological Characteristics of Cotesia glomerata L. (Hymenoptera: Braconidae) and Its Parasitism Rates for Diamondback Moth (Plutella xylostella L.) in a Kimchi Cabbage Field in The Korean Highland Area (배추나비고치벌(Cotesia glomerata L.)의 생태적 특성 및 고랭지 배추밭에서 배추좀나방(Plutella xylostella L.)에 대한 기생률)

  • Kwon, Min;Kim, Juil;Hong, Eunju;Lee, Yeonggyu
    • Korean journal of applied entomology
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    • v.58 no.4
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    • pp.355-362
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    • 2019
  • Cotesia glomerata L., an internal parasitoid wasp, attacks the larvae of both the cabbage white butterfly (Artogeia rapae L.) and the diamondback moth (Plutella xylostella L.). It can be utilized as a natural biological enemy to control these two insect pests in the summer cabbage fields of the Korean highland areas. The developmental response and sex ratio of C. glomerata to various temperatures and its longevity were examined in the laboratory. The egg-to-larva and pupa stages of C. glomerata were 12.1 ± 2.1 and 6.4 ± 1.8 days, respectively, at 20℃, The developmental threshold for egg-to-larva and pupa stages were 7.7 and 8.5℃, respectively. The sex ratios of C. glomerata when reared under various temperatures were 61.0 ± 4.5% at 15℃, 44.2 ± 1.0% at 20℃, and 39.0 ± 2.3% at 25℃, and the incidence of females increased as the temperature decreased. The longevity of C. glomerata when fed a 10% sugar solution was 20.4 ± 0.2 days, while in adults without any feed, the longevity was 3.6 ± 0.1 days. Indoor reared C. glomerata adults were released into cabbage fields from 2007 to 2018, in early August of each year, and the outdoor parasitism rates were surveyed. The parasitism rates were found to increase gradually as the year passed (Y = 0.2696X + 2.8633, R2 = 0.3994). The highest parasitism rate was observed in 2013 at 7.6%, and the lowest was in 2018 at 6.5 %. These results could be used as basic information for biological control of kimchi cabbage pests at highland fields.

Molecular Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis (ITS2 rDNA 염기서열 분석을 통한 Trichogramma 속(벌목: 알벌과)의 조명나방 알기생벌에 대한 종 추정)

  • Seo, Bo Yoon;Jung, Jin Kyo;Park, Ki Jin;Cho, Jum Rae;Lee, Gwan-Seok;Jung, Chung Ryul
    • Korean journal of applied entomology
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    • v.53 no.3
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    • pp.247-260
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    • 2014
  • To identify the species of Trichogramma occurring in the corn fields of Korea as egg parasitoids of Ostrinia furnacalis, we sequenced the full-length of ITS2 nuclear rDNA from 112 parasitoids collected during this study. As a reference to distinguish species, we also retrieved full-length ITS2 sequences of 60 Trichogramma species from the NCBI GenBank database. On the basis of the size and 3'terminal sequence pattern of the ITS2 sequences, the Trichogramma samples collected in this study were divided into three groups (K-1, -2, and -3). Evolutionary distances (d) within and between groups based on ITS2 sequences were estimated to be ${\leq}0.005$ and ${\geq}0.080$, respectively. In the net average distance between groups or species, the d value between K-1 and T. ostriniae, K-2 and T. dendrolimi, and K-3 and T. confusum was the lowest, with values of 0.016, 0.001, and 0.002, respectively. In the phylogenetic tree, K-1 and K-2 were clustered with T. ostriniae and T. dendrolimi, respectively. However, K-3 was clustered with three different species, namely, T. confusum, T. chilonis, and T. bilingensis. NCBI BLAST results revealed that parasitoids belonging to K-1 and K-2 showed 99% identity with T. ostriniae and T. dendrolimi, respectively. Parasitoids in K-3 collected from Hongcheon showed 99-100% identity with T. confusum and T. chilonis, and one parasitoid in K-3 collected from Gochang had 98% identity with T. bilingensis, T. confusum, and T. chilonis. On the basis of these results, we infer that the species of Trichogramma collected in this study are closely related to T. ostriniae (K-1) and T. dendrolimi (K-2). However, it was not possible to distinguish species of K-3 using the ITS2 sequence alone.