• Animal Bioscience
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• v.37 no.8
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• pp.1418-1427
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• 2024

Objective: This study aimed to investigate the efficacy of Bacillus-based probiotics supplemented at two different levels to modulate the productive performance, egg quality, tibia traits, and specific cecal bacteria counts of Hy-Line Brown layers from 25 to 37 weeks of age. Methods: A total of 216 twenty-five-week-old hens were randomly distributed into 3 experimental diets with 12 replicates of 6 birds per cage. Diets included basal diet supplemented with 0 (CON), 3×10⁸ (PRO1), or 3×10⁹ (PRO2) colony-forming unit (CFU) of the test probiotic containing Bacillus subtilis PB6, Bacillus subtilis FXA, and Bacillus licheniformis G3 per kilogram of feed. Results: Improved egg weights and mass at 29 weeks; and feed intake at 31 weeks (p<0.10) were noticed with the probiotic-supplemented PRO1 and PRO2 diets. Considering egg quality, the shell thickness, Haugh units, and yolk color were improved; but yolk cholesterol was lowered (p<0.05) with PRO1 and PRO2 diets at 29 weeks. At both 33 and 37 weeks, the egg-breaking strength, shell color and thickness, albumen height, Haugh units, and yolk color were improved; but yolk cholesterol was similarly lowered (p<0.05) with the PRO1 and PRO2 diets. Improved tibia Ca, ash, weights, and density; and raised cecal counts of Bifidobacteria and Lactobacilli (p<0.05) were noticed with PRO1 and PRO2 diets. Improved tibia P but reduced Clostridia counts (p<0.10) were also observed with the PRO1 and PRO2 diets. Conclusion: Probiotic supplementation of Bacillus subtilis PB6, Bacillus subtilis FXA, and Bacillus licheniformis G3 at 3×10⁸ CFU/kg of feed is adequate to significantly improve egg quality, lower yolk cholesterol, enhance several tibia traits, and raise the populations of beneficial cecal bacteria. Modest improvements in several productive parameters and tibia P but reduced Clostridia were also observed; and could warrant further investigation of probiotic effects beyond the current test period.

• Toxicological Research
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• v.17
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• pp.197-199
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• 2001

Enhancement of antigen-specific IgE is a hallmark of allergic hyperresponsiveness, therefore it is necessary to adopt or develop a highly sensitive and specific assay for determination of allergen-specific IgE levels in vivo. In this presentation, we introduce an ELISA (enzyme linked immunosorbent assay) system developed to measure the levels of chicken egg ovalbumin (OVA)-specific IgE in serum. The ELISA method uses a commercially available purified rat anti-mouse IgE as a capture Ab and biotinylated OVA as a detection reagent. Avidin-peroxidase with its substrate is used for color development resulting in optical density measurement at 405 nm. The ELISA system produces a highly sensitive dose-response relation-ship between optical density levels and the dilution titer of the OVA-IgE standard serum but no cross-reaction with unrelated IgE or IgG. It is believed that the system is an Efficient tool to delineate an adjuvant effect of environmental pollutants on development of asthmatic and atopic responses.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.11
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• pp.1622-1629
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• 2013

The objective of this study was to develop a new method to separate phosvitin from egg yolk without using organic solvents. Phosvitin was extracted from yolk granules using 10% NaCl or 10% $(NH_4)_2SO_4$ (final concentration) and then treated with heat to precipitate the lipoproteins from the extracted solution. The optimal pH for the phosvitin extraction from yolk granules was determined, and the iron-binding ability of the extracted phosvitin (final product) was tested. Adding 10% $(NH_4)_2SO_4$ disrupted the granules, and the subsequent thermal treatment at $90^{\circ}C$ for 1 h precipitated low density and high density lipoproteins, which enabled separation of phosvitin by centrifugation. The phosvitin concentration in the extract was significantly higher when the pH of the solution was adjusted to pH ${\geq}9$. The purity and recovery rate of phosvitin at the end of the separation process were approximately 78% and 56%, respectively. The separated phosvitin was confirmed to have ferrous and ferric iron binding ability. The advantages of this new method compared with the traditional methods include no organic solvents and high-priced equipment are needed for the separation. Also, this method is more environment and consumer friendly than that of the traditional methods.

• Korean journal of applied entomology
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• v.34 no.3
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• pp.234-242
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• 1995

The functional responses of the female Cyrtorhinus lividipennis on brown planthopper (BPH), Nilaparvata lugens, eggs and their predation behavior were investigated at six temperature conditions; 20, 23, 26, 29, 32, and $35^{\circ}C$. C. lividipennis was found to prefer young BPH eggs, especially 3-day-old eggs the most. The functional responses for female C. lividipennis on BPH eggs fitted the Holling's Type-I true predator-prey Rogers' (1972) model at all temperatures tested except $35^{\circ}C$ at which the negative Th value was produced. With the temperature increased up to 32$^{\circ}C$, the instantaneous attacking rate(a) increased from 0.1923 at $20^{\circ}C$ to 0.5085 at $32^{\circ}C$, while the handling time (Th) was gradually decreased as low as 0.0151 at $32^{\circ}C$. C. lividipenis preferred the BPH eggs laid on the upper part of rice stem when the BPH egg density was high, but there was no significant difference in the preference when the egg density was low. The preference was more obvious in high temperature conditions such as above 29$^{\circ}C$.

• Korean journal of applied entomology
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• v.32 no.3
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• pp.279-284
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• 1993

This experiment was conducted to investigate the seasonal fluctuation of population density and the life history of Adoxophyes sp .. Under field-cage conditions, smaller tea tortrix had 4-5 generations a year. The average periods from egg to adult emergence were 43.7 days in spring and fall, and 35.2 days in summer. The average longevity of adults was 7.0 days in spring and fall, and 6.3 days in summer. The average numbers of egg masses and eggs laid by a female were 1.8 and 40.4 m spring and fall, and 2.3 and 49.5 in summer, respectively. Population density of smaller tea tortrix estimated by sex pheromone trap and light trap showed four peaks in each late May, late June, middle August and late September. The number of individuals caught by sex pheromone trap was 2~4 times as large as that by jight trap. Smaller tea tartrix overwimered in larval stage in southern region af Korea.

• Journal of Electrochemical Science and Technology
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• v.13 no.4
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• pp.497-503
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• 2022

Spinel ferrites (Ni_xFe_3-xO₄; x = 0.25, 0.5, 0.75 and 1.0) have been prepared at 550℃ by egg white auto-combustion route using egg white at 550℃ and characterized by physicochemical (TGA, IR, XRD, and SEM) and electrochemical (CV and Tafel polarization) techniques. The presence of characteristic vibration peaks in FT-IR and reflection planes in XRD spectra confirmed the formation of spinel ferrites. The prepared oxides were transformed into oxide film on glassy carbon electrodes by coating oxide powder ink using the nafion solution and investigated their electrocatalytic performance for OER in an alkaline solution. The cyclic voltammograms of the oxide electrode did not show any redox peaks in oxygen overpotential regions. The iR-free Tafel polarization curves exhibited two Tafel slopes (b₁ = 59-90 mV decade^-1 and b₂ = 92-124 mV decade^-1) in lower and higher over potential regions, respectively. Ni-substitution in oxide matrix significantly improved the electrocatalytic activity for oxygen evolution reaction. Based on the current density for OER, the 0.75 mol Ni-substituted oxide electrode was found to be the most active electrode among the prepared oxides and showed the highest value of apparent current density (~9 mA cm^-2 at 0.85 V) and lowest Tafel slope (59 mV decade^-1). The OER on oxide electrodes occurred via the formation of chemisorbed intermediate on the active sites of the oxide electrode and follow the second-order mechanism.

• Korean Journal of Agricultural Science
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• v.42 no.1
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• pp.7-13
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• 2015

Ninety genes randomly selected from tobacco whitefly (Bemisia tabaci) cDNA library was studied for selecting target gene in order to control of tobacco whitefly using TRV-VIGS vector (tobacco rattle virus-virus induced gene silencing vector) with RNAi. First of all, the occurrence of B. tabaci adult according to agro-infiltration of TRV was no significant difference. And that of TRV inserted tobacco whitefly cDNA showed a significant difference in each sample. P CV and N CV sample were more than 80% could be confirmed in 5 samples, for example, wh11, wh36, wh46, wh50 and wh71. Lastly, the occurrence of nymph and egg also showed a significant difference in each sample. That could be confirmed in 11 samples, for example, wh01, wh09, wh10, wh15, wh16, wh23, wh24, wh48, wh64 and wh66. In case of wh46, wh50 and wh71 sample could be confirmed that occurrence of B. tabaci adult was many, but occurrence of B. tabaci nymph and egg was a little. So sample showed a physioecological good effect to control of whitefly need to be investigated variation of gene expression in whitefly body using qRT-PCR through individual test.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.25 no.6
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• pp.495-500
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• 1992

The relationship between distribution of eggs and larvae of anchovy, Engraulis japonica, and environmental factors were studied using the data on the ichthyoplankton and zooplankton sampled vertically by net, and water temperature and salinity observed by CTB in the eastern waters of Korea in every two month in 1985. Anchovy eggs and larvae were occurred in June and August. They distributed in the warm water current which was high temperature and salinity in June. Egg and larval distribution area were moved to the offshore in August. It is likely that distribution of anchovy eggs and larval in June significantly correlate with hydroconditions in the eastern waters of Korea.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.4
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• pp.582-586
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• 2006

This experiment was conducted to investigate the dietary effect of garlic powder (GP) and copper (Cu) on laying performances and the cholesterol content and quality characteristics of eggs during storage in laying hens. A total of one hundred and eighty, 50-wk-old, Hy-Line Brown layers were divided into 6 groups with 3 replicates per group (10 layers per replicate) and fed one of six diets containing GP 0%, GP 1%, GP 3%, GP 5%, Cu 200 ppm, or GP 3%-Cu 200 ppm for 5 wks. There were no differences in the laying performances and feed intakes bertween treatments. Eggshell strength, eggshell thickness and yolk color were also not affected by feeding of GP and Cu. With increasing dietary GP, Haugh unit was linearly increased after 2 wk of storage (p<0.05). The levels of serum total cholesterol in hens fed diets containing GP or Cu were lower than that of the control (p<0.05), but high density lipoprotein-cholesterol was not influenced by dietary GP or Cu. The content of egg yolk cholesterol from hens fed diets containing GP or Cu was significantly decreased from that of the control, except for the GP 1% group. Based on the results of this experiment, the decrease of Haugh unit during storage was alleviated by feeding of GP. The feeding of GP or Cu alone and in combination altered the cholesterol fractions in serum and reduced the content of egg yolk cholesterol in laying hens.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.54 no.3
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• pp.262-270
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• 2018

We examined the characteristics of egg and larval distributions and catch changes of anchovy in relation to abnormally high sea temperature in the South Sea of Korea in summer 2015 and 2016. The densities of anchovy eggs and larvae in the southern coastal region were lower in July-August 2016 than in July-August 2015. In particular, anchovy eggs and larvae (approximately 5 mm TL) were rarely observed in the coastal region in August 2016 due to the abnormally high SST (up to $28^{\circ}C$), which was above the optimum spawning temperature of anchovy. The catch of non-swimming stage (< 2 cm TL) larval anchovy was lower in July-August 2016 than in July-August 2015. The decreased catch of larval anchovy in July-August 2016 could be attributed to decreased spawning density in June-July 2016. In contrast, the catch of swimming stage (> 2 cm TL) anchovy was increased in July-August 2016. In the summer of 2016, prominent sea temperature near the southern coast of Korea and sea temperature higher than $30^{\circ}C$ in the offshore region of the South Sea of Korea could greatly enhance the retention of swimming anchovies in the coastal fishing grounds.