• Bulletin of the Korean Chemical Society
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• v.31 no.7
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• pp.1843-1847
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• 2010

An efficient method for the preparation of 1,4-disubstituted 1,2,3-triazole compounds is described using polymeric quaternary ammonium salts having azide or alkyne functionality to remove unreacted excess starting molecules (azide/alkyne). Copper metal could easily be removed by simple filtration with a short $Na_2SO_4$/silica cartridge, affording highly regioselective products in high yield and excellent purity without the need for work-up, extraction and chromatographic purification.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.84-84
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• 2013

FcBP consisting of 13 amino acids specifically binds to Immunoglobulin G Fc domain. Initially, we utilized this peptide for preparation of antibody chip as a PEG composite for enhanced solubility. After then, the peptide conjugate was immobilized on agarose resin, resulting in highly efficient affinity column for antibody purification. The efficiency was comparable to commercial Protein A column. Recently, this peptide was conjugated with cell penetratingpeptide (CPP) on a backbone of GFP, affording antibody transducer, which carries antibody into live cells by simple mixing of antibody and the transducer in cell culture media. Antibody transduction into cells was monitored by live cell imaging. More recently, the FcBP was fused to ferritin cage, which consists of 24 ferritin protein molecules. The FcBP-ferritin cage showed greatly increased binding affinity to human IgG. Its binding was analyzed by QCM and SPR analysis. Finally, it was selectively delivered by Herceptin to SKBR3, a breast cancer cell, over MCF10A, non-tumorigenic cells.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.2 no.2
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• pp.137-142
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• 2016

We have successfully synthesized of 4-cyano-1-(2,4-dichlorophenyl)-5-(4-[$^{11}C$]methoxyphenyl)-N-(piperidin-1-yl)-1H-pyrazole-3-carboxzmide ([$^{11}C$]OMAR), which has been shown a progressing candidate to human brain PET study, from fully automated loop method using ethanol as the only solvent for the entire manufacturing process. The radiochemical yield of [$^{11}C$]OMAR was observed in $4.1{\pm}0.2%$ with $4990{\pm}384Ci/mmol$ of the specific activity and total synthesis time was about 45 minutes after HPLC purification (n = 3, decay corrected) from ethanolic loop system, which was exhibited to better results compared with conventional methods. Ethanolic loop chemistry is favorable and efficient method by simplifies manufacturing procedures as well as satisfied suitable for human administration.

• Ceramist
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• v.22 no.3
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• pp.230-242
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• 2019

Functional ceramics are widely utilized in a variety of application fields such as structural materials, sensors, energy devices, purification filter and etc due to their high strength, stability and chemical activity. With the breakthrough development of nanotechnology, many researchers have studied new types of nanomaterials including nanoparticle, nanorod, nanowire and nanoplate to realize high-performance ceramics. Especially several groups have focused on the 3D nanostructured ceramics because of their large surface area, efficient load transfer, ultra-fast ion diffusion and superior electrical (or thermal) conductivity. In this review, we introduce the reported fabrication strategies of the 3D nanostructured and functional ceramics, also summarized the 3D nanostructured ceramic based high-performance applications containing photocatalysts, structural materials, energy harvesting and storage devices.

• Journal of Microbiology and Biotechnology
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• v.5 no.4
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• pp.238-240
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• 1995

A novel and simple purification method for microbial $poly-{\beta}-hydroxybutyrate$ (PHS) was developed. Sodium hydroxide was found to be efficient for digesting cell materials. Initial biomass concentration, NaOH concentation, digestion time, and incubation temperature were optimized. When 40 g/l of biomass was incubated in 0.1 N NaOH at $30^{\circ}C$ for 1 h, PHB purity of 88.4% with a weight average molecular weight ($M_w$) of 770,000 and a polydispersity index (PI) of 2.4 was recovered with a yield of 90.8% from the biomass which initially contained PHB of a $M_w$ of 780,000 and a PI of 2.3.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.500-503
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• 2002

In order to improve the physical properties of food such as texture and food self-life. Transglutaminase(mTG) from Streptoverticillum morbarense was prepared. In the preliminary experiments, presence of proteases in the crude enzyme did not improve the texture of dough, which mean the inteference of mTG reaction by the proteases. Among the cation exchange resins tested for the removal of proteases, Monoplus S 100(Bayer, Germany) was the most efficient resin with 20 fold increase in the mTG/protease activity ratio. By further purification steps with a quaternary ammonia salt resin and a gel permeation chromatography, proteases were effectively removed from the preparation. Therefore, the improvement of flour texture was shown by adding the protease-free mTG.

• Journal of Microbiology and Biotechnology
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• v.20 no.11
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• pp.1481-1483
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• 2010

We constructed an efficient T-vector, pTQEST216T that employed an engineered esterase as an indicator for direct cloning of PCR products. After ligation of the XcmI-digested vector with PCR products, this cloning system could easily discriminate positive clones owing to insertional inactivation of the esterase reporter. Additionally, PCR products were efficiently cloned into this vector without the gel purification steps, owing to the well-designed multi-cloning site that was in-frame fused at the circularly permutated gap of the reporter.

• Microbiology and Biotechnology Letters
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• v.41 no.1
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• pp.79-87
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• 2013

An unstable yet efficient phenanthrene-degrading bacterium strain Ph-3 was isolated from a petroleum-contaminated site at the Mathura Oil Refinery, India. The strain was identified as Pseudomonas sp. using a polyphasic approach. An analysis of the intermediates and assays of the degradative enzymes from a crude extract of phenanthrene-grown cells showed a novel and previously unreported pattern of 1, 2-dihydroxy naphthalene and salicylic acid production. While strain Ph-3 lost its phenanthrene- degrading potential during successive transfers on a rich medium, it maintained this trait in oligotrophic soil conditions under the stress of the pollutant and degraded phenanthrene efficiently in soil microcosms. Although the maintenance and in vitro study of unstable phenotypes are difficult and such strains are often missed during isolation, purification, and screening, these bacteria constitute a substantial fraction of the microbial community at contaminated sites and play an important role in pollutant degradation during biostimulation or monitored natural attenuation.

• Journal of the Korean Chemical Society
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• v.52 no.1
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• pp.52-56
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• 2008

facile and efficient method for the preparation of Z-aldoximes is improved by means of H3PMo12O40 catalyst in solvent-free media. The major advantages of this method are: operational simplicity, low catalyst loading, selectivity, mild reaction conditions, short reaction times and excellent yields. The recovered catalyst could be used in new attempts without any purification.

• Bulletin of the Korean Chemical Society
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• v.29 no.7
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• pp.1332-1334
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• 2008

Stable crystalline 1-Benzyl-4-aza-1-azonia-bicyclo[2.2.2]octane tribromide (BABOT), can be readily synthesized from the reaction of the corresponding bromide with $HNO_3$ and aqueous KBr. Selective Oxidation of a variety of dialkyl and alkyl Aryl sulfides to the corresponding sulfoxides in high yield was achieved by this reagent in solution ($CH_3CN/H_2O$) and solvent free conditions. The reaction proceeds under neutral and mild conditions and can be carried out easily at room temperature with regeneration of BABOT. In this method purification of products is straightforward and no over oxidation to sulfone was noted.