• Title/Summary/Keyword: Effective competition

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Field Studios of In-situ Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbons

  • Semprini, Lewts
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.3-4
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    • 2004
  • Results will be presented from two field studies that evaluated the in-situ treatment of chlorinated aliphatic hydrocarbons (CAHs) using aerobic cometabolism. In the first study, a cometabolic air sparging (CAS) demonstration was conducted at McClellan Air Force Base (AFB), California, to treat chlorinated aliphatic hydrocarbons (CAHs) in groundwater using propane as the cometabolic substrate. A propane-biostimulated zone was sparged with a propane/air mixture and a control zone was sparged with air alone. Propane-utilizers were effectively stimulated in the saturated zone with repeated intermediate sparging of propane and air. Propane delivery, however, was not uniform, with propane mainly observed in down-gradient observation wells. Trichloroethene (TCE), cis-1, 2-dichloroethene (c-DCE), and dissolved oxygen (DO) concentration levels decreased in proportion with propane usage, with c-DCE decreasing more rapidly than TCE. The more rapid removal of c-DCE indicated biotransformation and not just physical removal by stripping. Propane utilization rates and rates of CAH removal slowed after three to four months of repeated propane additions, which coincided with tile depletion of nitrogen (as nitrate). Ammonia was then added to the propane/air mixture as a nitrogen source. After a six-month period between propane additions, rapid propane-utilization was observed. Nitrate was present due to groundwater flow into the treatment zone and/or by the oxidation of tile previously injected ammonia. In the propane-stimulated zone, c-DCE concentrations decreased below tile detection limit (1 $\mu$g/L), and TCE concentrations ranged from less than 5 $\mu$g/L to 30 $\mu$g/L, representing removals of 90 to 97%. In the air sparged control zone, TCE was removed at only two monitoring locations nearest the sparge-well, to concentrations of 15 $\mu$g/L and 60 $\mu$g/L. The responses indicate that stripping as well as biological treatment were responsible for the removal of contaminants in the biostimulated zone, with biostimulation enhancing removals to lower contaminant levels. As part of that study bacterial population shifts that occurred in the groundwater during CAS and air sparging control were evaluated by length heterogeneity polymerase chain reaction (LH-PCR) fragment analysis. The results showed that an organism(5) that had a fragment size of 385 base pairs (385 bp) was positively correlated with propane removal rates. The 385 bp fragment consisted of up to 83% of the total fragments in the analysis when propane removal rates peaked. A 16S rRNA clone library made from the bacteria sampled in propane sparged groundwater included clones of a TM7 division bacterium that had a 385bp LH-PCR fragment; no other bacterial species with this fragment size were detected. Both propane removal rates and the 385bp LH-PCR fragment decreased as nitrate levels in the groundwater decreased. In the second study the potential for bioaugmentation of a butane culture was evaluated in a series of field tests conducted at the Moffett Field Air Station in California. A butane-utilizing mixed culture that was effective in transforming 1, 1-dichloroethene (1, 1-DCE), 1, 1, 1-trichloroethane (1, 1, 1-TCA), and 1, 1-dichloroethane (1, 1-DCA) was added to the saturated zone at the test site. This mixture of contaminants was evaluated since they are often present as together as the result of 1, 1, 1-TCA contamination and the abiotic and biotic transformation of 1, 1, 1-TCA to 1, 1-DCE and 1, 1-DCA. Model simulations were performed prior to the initiation of the field study. The simulations were performed with a transport code that included processes for in-situ cometabolism, including microbial growth and decay, substrate and oxygen utilization, and the cometabolism of dual contaminants (1, 1-DCE and 1, 1, 1-TCA). Based on the results of detailed kinetic studies with the culture, cometabolic transformation kinetics were incorporated that butane mixed-inhibition on 1, 1-DCE and 1, 1, 1-TCA transformation, and competitive inhibition of 1, 1-DCE and 1, 1, 1-TCA on butane utilization. A transformation capacity term was also included in the model formation that results in cell loss due to contaminant transformation. Parameters for the model simulations were determined independently in kinetic studies with the butane-utilizing culture and through batch microcosm tests with groundwater and aquifer solids from the field test zone with the butane-utilizing culture added. In microcosm tests, the model simulated well the repetitive utilization of butane and cometabolism of 1.1, 1-TCA and 1, 1-DCE, as well as the transformation of 1, 1-DCE as it was repeatedly transformed at increased aqueous concentrations. Model simulations were then performed under the transport conditions of the field test to explore the effects of the bioaugmentation dose and the response of the system to tile biostimulation with alternating pulses of dissolved butane and oxygen in the presence of 1, 1-DCE (50 $\mu$g/L) and 1, 1, 1-TCA (250 $\mu$g/L). A uniform aquifer bioaugmentation dose of 0.5 mg/L of cells resulted in complete utilization of the butane 2-meters downgradient of the injection well within 200-hrs of bioaugmentation and butane addition. 1, 1-DCE was much more rapidly transformed than 1, 1, 1-TCA, and efficient 1, 1, 1-TCA removal occurred only after 1, 1-DCE and butane were decreased in concentration. The simulations demonstrated the strong inhibition of both 1, 1-DCE and butane on 1, 1, 1-TCA transformation, and the more rapid 1, 1-DCE transformation kinetics. Results of tile field demonstration indicated that bioaugmentation was successfully implemented; however it was difficult to maintain effective treatment for long periods of time (50 days or more). The demonstration showed that the bioaugmented experimental leg effectively transformed 1, 1-DCE and 1, 1-DCA, and was somewhat effective in transforming 1, 1, 1-TCA. The indigenous experimental leg treated in the same way as the bioaugmented leg was much less effective in treating the contaminant mixture. The best operating performance was achieved in the bioaugmented leg with about over 90%, 80%, 60 % removal for 1, 1-DCE, 1, 1-DCA, and 1, 1, 1-TCA, respectively. Molecular methods were used to track and enumerate the bioaugmented culture in the test zone. Real Time PCR analysis was used to on enumerate the bioaugmented culture. The results show higher numbers of the bioaugmented microorganisms were present in the treatment zone groundwater when the contaminants were being effective transformed. A decrease in these numbers was associated with a reduction in treatment performance. The results of the field tests indicated that although bioaugmentation can be successfully implemented, competition for the growth substrate (butane) by the indigenous microorganisms likely lead to the decrease in long-term performance.

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Application of Montmorillonite as Capping Material for Blocking of Phosphate Release from Contaminated Marine Sediment (해양오염퇴적물 내 인산염 용출차단을 위한 피복소재로서의 몬모릴로나이트 적용)

  • Kang, Ku;Kim, Young-Kee;Hong, Seong-Gu;Kim, Han-Joong;Park, Seong-Jik
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.8
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    • pp.554-560
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    • 2014
  • To investigate the applicability of montmorillonite to capping material for the remediation of contaminated marine sediment, adsorption characteristics of $PO{_4}{^{3-}}$ onto montmorillonite were studied in a batch system with respect to changes in contact time, initial concentration, pH, adsorbent dose amount, competing anions, adsorbent mixture, and seawater. Sorption equilibrium reached in 1 h at 50 mg/L but 3 h was required to reach sorption equilibrium at 300 mg/L. Freundlich model was more suitable to describe equilibrium sorption data than Langmuir model. The $PO{_4}{^{3-}}$ adsorption decreased as pH increased, due to the $PO{_4}{^{3-}}$ competition for favorable adsorption site with OH- at higher pH. The presence of anions such as nitrate, sulfate, and bicarbonate had no significant effect on the $PO{_4}{^{3-}}$ adsorption onto the montmorillonite. The use of the montmorillonite alone was more effective for the removal of the $PO{_4}{^{3-}}$ than mixing the montmorillonite with red mud and steel slag. The $PO{_4}{^{3-}}$ adsorption capacity of the montmorillonite was higher in seawater than deionized water, resulting from the presence of calcium ion in seawater. The water tank elution experiments showed that montmorillonite capping blocked well the elution of $PO{_4}{^{3-}}$, which was not measured up to 14 days. It was concluded that the montmirillonite has a potential capping material for the removal of the $PO{_4}{^{3-}}$ from the aqueous solutions.

Implementation of TRL and TRA tools to Korean Construction and Transportation R&D Evaluation for Improving Practical Use (국가 건설교통 R&D 사업의 실용화 향상을 위한 기술성숙도평가 기반 R&D 평가모델 수립에 관한 연구)

  • Kim, Nam-Gyun;An, Byung-Ho;Lee, Hyun-Seok;Choi, Jae-Hong;Park, Sang-Hyuk;Kim, Yea-Sang
    • Korean Journal of Construction Engineering and Management
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    • v.13 no.4
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    • pp.110-119
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    • 2012
  • Recently as the advent of knowledge-based competition, the major industrialized countries including Korea, are expanding their R&D investment and promoting policies for diffusing of R&D performance. For this, NASA, DoD and such agencies in the United States define Technology Readiness Level (TRL) as a quantified indicator for performance management, and also impose Technology Readiness Assessment (TRA) as a evaluation system, that evaluates technology maturity using TRL. These tools are judged to be very effective in R&D projects especially with the object of practical use. When this concept is applied to the domestic national construction and transportation R&D project for the purpose of practical use, it is expected that the evaluation system overcomes its weakness of focusing only on academic results and improves significantly on intent of achieving practical use. Thus in this study, TRL/TRA implementation model of construction and transportation R&D project for achieving purpose of practical use is presented through an analysis of TRL/TRA concept and domestic national construction and transportation R&D project's characteristics and current evaluation/management system.

Seeding of the Woody Plants for the Quick-coverage of the Slopes (비탈면 조기수림화를 위한 녹화용 식물의 활용에 관한 연구)

  • Kim, Namchoon;Yoon, Jungseo;Bae, Sunwoo;Son, Won-Ju;Jung, Sungchul
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.5 no.6
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    • pp.72-85
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    • 2002
  • This study was conducted to investigate the germination rates and ground coverage rates of the 16 native wild flower, herbaceous, shrub and woody plants according to temperature and seeding timing for the revegetation and rehabilitation of the roadside slopes. Also, this study was conducted to suggest design criteria to select revegetation plants and to decide proper seed mixture for ecological restoration of the disturbed manmade slopes as a environmentally friendly construction. The results are summarized as follows. 1. Most of the plants germinated after 10~14 days. Woody plants germinated more slowly than wild flowers and herbaceous plants because of the hard seed. 2. Most of the plants showed the highest percentage of the germination rates under $15/25^{\circ}C$ temperature. The next was in order of under the $20/30^{\circ}C$ temperature, normal temperature(October) and $25/35^{\circ}C$ temperature. 3. At the chamber experiment, the Sorbaria sorbifolia var. stellipila showed the highest germination rates. Most of the native wild flowers germinated well and showed high germination rates under the various temperature. 4. As the field germination experiments, the seeding at August shows higher germination rates than that of the seeding at September, but showed lower surviving percentage of the germinated seedlings and lower ground coverage percentage than those of the seeding at September. After one year, Chionanthus retusus, Acer palmatum, Albizzia julibrissin are germinated and showed 10% coverage rates. So, they can be used as revegetation plants for the restoration works. 5. As seed mixture experiment, it was not effective only to increase the proportion of the ratio of the tree seeds, relatively high price, for making woody vegetation. It would be more successful to make vegetation structure by natural competition among wild flowers, shrubs and trees. 6. The excessive dominance of trees in revegetation works may destroy the lower layer of vegetation and it will be undesirable on the species diversity. It is more important to recover the species diversity of the plant community by seed mixture with the considering the germination, the growth characteristics and the correlation effect among revegetation plants. 7. To recover the manmade slopes quickly, increasing the ratio of the wild flower was recommended.

Definition of the peptide mimotope of cellular receptor for hepatitis C virus E2 protein using random peptide library (Random peptide library를 이용한 C형 간염바이러스 E2 단백질 세포막 수용체의 peptide mimotope 규명)

  • Lee, In-Hee;Paik, Jae-Eun;Seol, Sang-Yong;Seog, Dae-Hyun;Park, Sae-Gwang;Choi, In-Hak
    • IMMUNE NETWORK
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    • v.1 no.1
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    • pp.77-86
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    • 2001
  • Background: Hepatitis C virus(HCV), a family of Flaviviridae, has a host cell-derived envelope containing a positive-stranded RNA genome, and has been known as the maj or etiological agent for chronic hepatitis, hepatic cirrhosis, and hepatocellular carcinoma. There remains a need to dissect a molecular mechanism of pathogenesis for the development of therapeutic and effective preventive measure for HCV. Identification of cellular receptor is of central importance not only to understand the viral pathogenesis, but also to exploit strategies for prevention of HCV. This study was aimed at identifying peptide mimotopes inhibiting the binding of E2 protein of HCV to MOLT-4 cell. Methods: In this study, phage peptide library displaying a random peptides consisting of 7 or 12 random peptides was employed in order to pan against E2 protein. Free HCV particles were separated from the immune complex forms by immunoprecipitation using anti-human IgG antibody, and used for HCV-capture ELISA. To identify the peptides inhibiting E2-binding to MOLT-4 cells, E2 protein was subj ect to bind to MOLT-4 cells under the competition with phage peptides. Results: Several phage peptides were selected for their specific binding to E2 protein, which showed the conserved sequence of SHFWRAP from 3 different peptide sequences. They were also able to recognize the HCV particles in the sera of HCV patients captured by monoclonal antibody against E2 protein. Two of them, showing peptide sequence of HLGPWMSHWFQR and WAPPLERSSLFY respectively, were revealed to inhibit the binding of E2 protein to MOLT-4 cell efficiently in dose dependent mode. However, few membrane-associated receptor candidates were seen using Fasta3 programe for homology search with these peptides. Conclusion: Phage peptides containing HLGPWMSHWFQR and WAPPLERSSLFY respectively, showed the inhibition of E2-binding to MOLT-4 cells. However, they did not reveal any homologues to cellular receptors from GenBank database. In further study, cellular receptor could be identified through the screening of cDNA library from MOLT-4 or hepatocytes using antibodies against these peptide mimotopes.

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Exploring the Effects of Grouping by Learning Style of Gifted-Student in Science on the Verbal Interaction (과학 영재들의 학습양식에 따른 소집단 구성이 언어적 상호작용에 미치는 영향 탐색)

  • Lee, Eun-Kyung;Yoon, Jihyun;Kang, Seong-Joo
    • Journal of the Korean Chemical Society
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    • v.58 no.4
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    • pp.406-417
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    • 2014
  • The importance of small group activity has recently been emphasized in the gifted education in science because of the increased needs to foster the human resources that could explore through the communication and collaboration. In this study, we investigated the characteristics of the verbal interaction according to the learning styles of the gifted-students in science in the small group activity and examined how learning style affected the interaction within a group in order to seek an effective grouping strategy. The competition, cooperative, and dependent students with learning style in the small group 1 and the cooperative, cooperative, dependent students in the small group 2 were assigned by the 6 science high school students. The analyses of the results revealed that the small group 1 showed the asymmetric interaction of the low level, whereas the small group 2 showed the symmetric interaction of the high level. In other words, the frequencies of in-depth interaction in the small group 2 were higher than those in the small group 1, and also students in the small group 2 were equally involved in the activity rather than the small group 1. These results suggested that the grouping by the students' learning styles in the small group activity should affect significantly the participation decision in activity and the level of verbal interaction. Educational implications of theses findings were discussed.

An analysis of the operational efficiency of the major airports worldwide using DEA and Malmquist productivity indices (세계 주요 공항 운영 효율성 분석: DEA와 Malmquist 생산성 지수 분석을 중심으로)

  • Kim, Hong-Seop;Park, Jeong-Rim
    • Journal of Distribution Science
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    • v.11 no.8
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    • pp.5-14
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    • 2013
  • Purpose - We live in a world of constant change and competition. Many airports have specific competitiveness goals and strategies for achieving and maintaining them. The global economic recession, financial crises, and rising oil prices have resulted in an increasingly important role for facility investment and renewal and the implementation of appropriate policies in ensuring the competitive advantage for airports. It is thus important to analyze the factors that enhance efficiency and productivity for an airport. This study aims to determine the efficiency levels of 20 major airports in East Asia, Europe, and North America. Further, this study also suggests suitable policies and strategies for their development. Research design, data, and methodology - This paper employs the DEA-CCR, DEA-BCC, and DEA-Malmquist production index analysis models to determine airport efficiency. The study uses data on the efficiency and productivity of the world's leading airports between 2006 and 2010. The input variables include the airport size, the number of runways, the size of passenger terminals, and the size of cargo terminals. The output variables include the annual number of passengers and the annual cargo volume. The study uses basic data from the 2010 World Airport Traffic Report (ACI). The world's top 20 airports (as rated by the ACI report) are investigated. The study uses the expanded DEA Model and the Super Efficiency Model to identify the most effective airports among the top 20. The Malmquist productivity index analysis is used to measure airport effectiveness. Results - This study analyzes longitudinal and cross-sectional data on the world's top 20 airports covering 2006 to 2010. A CCR analysis shows that the most efficient airports in 2010 were Gatwick Airport (LGW), Zurich Airport (ZRH), Vienna Airport (VIE), Leonardo da Vinci Fiumicino Airport (FCO), Los Angeles International Airport (LAX), Seattle-Tacoma Airport (SEA), San Francisco Airport (SFO), HongKong Airport (HKG), Beijing Capital International Airport (PEK), and Shanghai Pudong Airport (PVG). We find that changes in airport productivity are affected more by technical factors than by airport efficiency. Conclusions - Based on the study results, we offer four airport development proposals. First, a benchmark airport needs to be identified. Second, inefficiency must be reduced and high-cost factors need to be managed. Third, airport operations should be enhanced through technical innovation. Finally, scientific demand forecasting and facility preparation must become the focus of attention. This paper has some limitations. Because the Malmquist productivity index is based on the hypothesis of the, the identified production change could be over- or under-estimated. Further, as DEA estimates the relative efficiency. It also cannot generalize to include all airport conditions because the variables are limited. To measure airport productivity more accurately, other input variables and environmental variables such as financial and policy factors should be included.

User Perspective Website Clustering for Site Portfolio Construction (사이트 포트폴리오 구성을 위한 사용자 관점의 웹사이트 클러스터링)

  • Kim, Mingyu;Kim, Namgyu
    • Journal of Internet Computing and Services
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    • v.16 no.3
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    • pp.59-69
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    • 2015
  • Many users visit websites every day to perform information retrieval, shopping, and community activities. On the other hand, there is intense competition among sites which attempt to profit from the Internet users. Thus, the owners or marketing officers of each site try to design a variety of marketing strategies including cooperation with other sites. Through such cooperation, a site can share customers' information, mileage points, and hyperlinks with other sites. To create effective cooperation, it is crucial to choose an appropriate partner site that may have many potential customers. Unfortunately, it is exceedingly difficult to identify such an appropriate partner among the vast number of sites. In this paper, therefore, we devise a new methodology for recommending appropriate partner sites to each site. For this purpose, we perform site clustering from the perspective of visitors' similarities, and then identify a group of sites that has a number of common customers. We then analyze the potential for the practical use of the proposed methodology through its application to approximately 140 million actual site browsing histories.

A Proposal for the Improvement Method of Order Production System in the Display Industry (디스플레이산업에서 수주생산방식의 개선 및 효율화 제고 방안)

  • Cho, Myong Ho;Cho, Jin Hyung
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.39 no.4
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    • pp.106-116
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    • 2016
  • MTO (Make to Order) is a manufacturing process in which manufacturing starts only after a customer's order is received. Manufacturing after receiving customer's orders means to start a pull-type supply chain operation because manufacturing is performed when demand is confirmed, i.e. being pulled by demand (The opposite business model is to manufacture products for stock MTS (Make to Stock), which is push-type production). There are also BTO (Build to Order) and ATO (Assemble To Order) in which assembly starts according to demand. Lean manufacturing by MTO is very efficient system. Nevertheless, the process industry, generally, which has a high fixed cost burden due to large-scale investment is suitable for mass production of small pieces or 'mass customization' defined recently. The process industry produces large quantities at one time because of the lack of manufacturing flexibility due to long time for model change or job change, and high loss during line-down (shutdown). As a result, it has a lot of inventory and costs are increased. In order to reduce the cost due to the characteristics of the process industry, which has a high fixed cost per hour, it operates a stock production system in which it is made and sold regardless of the order of the customer. Therefore, in a business environment where the external environment changes greatly, the inventory is not sold and it becomes obsolete. As a result, the company's costs increase, profits fall, and it make more difficult to survive in the competition. Based on the customer's order, we have built a new method for order system to meet the characteristics of the process industry by producing it as a high-profitable model. The design elements are designed by deriving the functions to satisfy the Y by collecting the internal and external VOC (voice of customer), and the design elements are verified through the conversion function. And the Y is satisfied through the pilot test verified and supplemented. By operating this make to order system, we have reduced bad inventories, lowered costs, and improved lead time in terms of delivery competitiveness. Make to order system in the process industry is effective for the display glass industry, for example, B and C groups which are non-flagship models, have confirmed that the line is down when there is no order, and A group which is flagship model, have confirmed stock production when there is no order.

A study on the development of simulation program for the small naturally aspirated four-stroke diesel engine (소형 4행정사이클 무과급 디이젤 기관의 성능 시뮤레이션 전산프로그램의 개발에 관한 연구)

  • 백태주;전효중
    • Journal of Advanced Marine Engineering and Technology
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    • v.8 no.1
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    • pp.17-36
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    • 1984
  • Since 1973, the competition on the development of fuel saving type internal combustion engines has become severe by the two times oil shock, and new type engines are reported every several months. Whenever these new type engines are developed, new designs are required and they will be offered in the market after performing the endurance test for a long time. But the engine market is faced with a heavy burden of finance, as the developing of a new engine requires tremendous expenses. For this reason, the computer simulation method has been lately developed to cope with it. The computer simulation method can be available to perform the reasonable research works by the theoretical analysis before carrying out practical experiments. With these processes, the developing expenses are cut down and the period of development is curtailed. The object of this study is the development of simulation computer program for the small naturally aspirated four-stroke diesel engine which is intended to product by the original design of our country. The process of simulation is firstly investigated for the ideal engine cycle, and secondly for the real engine cycle. In the ideal engine cycle, each step of the cycle is simulated by the energy balance according to the first law of thermodynamics, and then the engine performance is calculated. In the real cycle imulation program, the injection rate, the preparation rate and the combustion rate of fuel and the heat transfer through the wall of combustion chamber are considered. In this case, the injection rate is supposed as constant through the crank angle interval of injection and the combustion rate is calculated by the Whitehouse-Way equation and the heat transfer is calculated by the Annand's equation. The simulated values are compared with measured values of the YANMAR NS90(C) engine and Mitsubishi 4D30 engine, and the following conclusions are drawn. 1. The heat loss by the exhaust gas is well agree with each other in the lower load, but the measured value is greater than the calculated value in the higher load. The maximum error rate is about 15% in the full load. 2. The calculated quantity of heat transfer to the cooling water is greater than the measured value. The maximum error rate is about 11.8%. 3. The mean effective pressure, the fuel consumption, the power and the torque are well agree with each other. The maximum error is occurred in the fuel consumption, and its error rate is about 7%. From the above remarks, it may be concluded that the prediction of the engine performance is possibly by using the developed program, although the program needs to reform by adding the simulation of intake and exhaust process and assumping more reliable mechanical efficiency, volumetric efficiency, preparation rate and combustion rate.

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