• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.533-538
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• 2003

To establish an effective and reliable technique of mutation by protoplast regeneration in doxorubicin overproducing industrial strain, it is essential to optimize the conditions for protoplast regeneration. $CaCO_3$ as buffer, the negative effect of glucose was still evident without significant changes in pH, ruling out acidity as responsible for the suppression of anthracycline production and suggesting a direct effect of glucose on antibiotic biosynthesis. Production of doxorubicin was improved in doxorubicin overproducing industrial strain (BR-Dox) when protoplast regenerated. BR-Dox4 and BR-Dox6 of BR-Dox derivatives improved doxorubicin production by 25.2 % and 12.2 %, respectively.

• Journal of Embryo Transfer
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• v.14 no.1
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• pp.39-46
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• 1999

To establish the optimal culture systems for production of transferable embryos in Korean Cattle, pregnancy rates of IVF-derived blastocysts according to different culture media, culture method and culture duration were compared. Development of IVF-derived embryos to blastocysts was most effective in YS medium group co-cultre with cumulus cells. Blastocysts cultured for 6 to 8 d in vitro showed higher hatching rate and good quality. Pregnancy rates after transfer of IVF-derived blastocysts cultured for 7 or 8 d were high. Through our experiments, it is considered that improvement of culture media and culture method is necessary for mass production of blastocysts with excellent of good quality in Korean Cattle.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2003.05a
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• pp.409-418
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• 2003

The dies & molds are a very economical production tool and a high value-added product because of its mass production capability compared to other production methods. Due to the very stiff international competitiveness, the industries meet many obstacles. At this moment, measured the status, the international trends and try to find the vision and strategy to promotion of the industry. In Korea, large portion of dies and molds are still low value-added and make large number of tools. In order to advance this industry to the high-tech area and gain competitiveness in the golbal marketplaces, effective means of resource investment and strategy should be properly provided.

• Korean Journal of Microbiology
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• v.26 no.2
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• pp.143-147
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• 1988

The effect of ginseng saponin on aflatoxin(AF) production by Aspergillus parasiticus NRRL2999 and the mutagenicity of produced aflatoxin. The production of aflatoxin were decreased by the addition of ginseng saponin and the most effective concentration was 0.05%. The ratio of aflatoxin $B_{1}$ and aflatoxin $G_{1}$ were not changed by the addition of ginseng saponin. For the nutagenicity test, Ames method were adopted. Mutagenicity of mycelial aflatoxin was decreased by the addition of ginseng saponin on TA98, but not changed on TA100. Mutagenicity of excreted aflatoxin to broth was slightly increased by the saponin on TA98, but decreased on TA100.

• Journal of Microbiology and Biotechnology
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• v.1 no.1
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• pp.79-83
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• 1991

The effects of phosphate concentration in the medium, feeding of biosynthetic precursor, and the addition of exogenous berberine on cell growth and berberine production were studied in cell suspension cultures of Thalictrum rugosum. The depletion of phosphate in the medium enhanced the specific productivity up to twofold with significant release of berberine into the medium. Extracellular berberine was 19% of the total in the culture without phosphate while it was 2-5% of total berberine in the culture with even low amounts of phosphate. Precursor feeding was not effective in enhancing alkaloid formation. Initial presence of exogenous berberine did not have much effect on cell growth and alkaloid production. It was found that the cells have the capacity to take up large quantities of berberine. When $500{\;}mg{\cdot}l^{-1}$ of berberine was added exogenously at the beginning, 81% of total berberine was found in the cells.

• The Journal of Korean Medicine
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• v.21 no.4
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• pp.248-252
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• 2000

The subject was a 70-year-old man who was suffering from productive cough after stroke. His symptoms were night cough, thick sputum production, reddened tongue and night sweat. The subject was diagnosed as having a deficiency of Yin, and was treated with Chungsangboha-tang(Qingshangbuxia-tang). A decrease in the severity of the subject's cough and sputum were observed, and $O_2$ saturation increased. In this case Chungsangboha-tang(Qingshangbuxia-tang) was effective on the patient with night cough, thick sputum production, reddened tongue and night sweat due to deficiency of Yin after stroke.

• Journal of Microbiology and Biotechnology
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• v.26 no.7
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• pp.1285-1289
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• 2016

A split-column photobioreactor (SC-PBR), consisting of two bubble columns with different sizes, was developed to enhance the photon utilization efficiency in an astaxanthin production process from Haematococcus lacustris. Among the two columns, only the smaller column of SC-PBR was illuminated. Astaxanthin productivities and photon efficiencies of the SC-PBRs were compared with a standard bubble-column PBR (BC-PBR). Astaxanthin productivity of SC-PBR was improved by 28%, and the photon utilization efficiencies were 28-366% higher than the original BC-PBR. The results clearly show that the effective light regime of SC-PBR could enhance the production of astaxanthin.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1997.04a
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• pp.957-961
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• 1997

In this paper, an assessment has been proceeded about component technology that stamping car panel can be designed and manufactured efficiently. Also this study investigated srandard model of CAD/CAM system in production process of automobile dies. Recently, motor companies are doing their best to increase productivity and to reduce production time. So to develop user-friendly and effective standard model of CAD/CAM system is very important. The obtained results will lead to the reductions in lead time and man-hour required for the design and manufacture of the automobile dies.

• Journal of Animal Environmental Science
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• v.4 no.2
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• pp.113-126
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• 1998

This study was conducted to develop pig-housings based on the forecasting models of swine production, the weather conditions, and so on in Korea. The Korean pig-housings were developed according to the following basis : 1. They should be suitable to domestic weather conditions. 2. They should be designed based on the forecasting models of swine production of farrow to finish operation among the forecasting models of swine production in Korea. 3. Proper environments should be offered to pigs according to the growth. 4. The environmental control, the treatment of swine wastewater, and so on should be interrelated. 5. Manual energy should be saved by effective arrangements of pig-housings. In the future, performance test of the Korean pig-housings and development of facility automation systems which are suitable to these should be accomplished.

• 제어로봇시스템학회:학술대회논문집
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• 1992.10b
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• pp.306-311
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• 1992

We propose software algorithms which provide the characteristics of acceleration/deceleration essential to high dynamic performance at the transient state where industrial robots or CNC machine tools start and stop. The path error, which is one of the most significant factors in performance evaluation of industrial robots and CNC machine tools, is analyzed for linear, exponential, and parabolic acceleration/deceleration algorithms in case of circular interpolation. The analysis shows that the path error depends on the acceleration/deceleration routine and the servo control system. In experiments, the entire control algorithm including the proposed acceleration/deceleration algorithms is executed on the motion control system with a floating point digital signal processor(DSP) TMS320C30 as a CPU. The experimental results demonstrate that the proposed algorithms are very effective in controlling axes of motion of industrial robots or CNC machine tools with the desired characteristics.