• Journal of Information Processing Systems
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• v.19 no.1
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• pp.67-79
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• 2023

In the image field, data augmentation refers to increasing the amount of data through an editing method such as rotating or cropping a photo. In this study, a generative adversarial network (GAN) image was created using CycleGAN, and various colors of dogs were reflected through data augmentation. In particular, dog data from the Stanford Dogs Dataset and Oxford-IIIT Pet Dataset were used, and 10 breeds of dog, corresponding to 300 images each, were selected. Subsequently, a GAN image was generated using CycleGAN, and four learning groups were established: 2,000 original photos (group I); 2,000 original photos + 1,000 GAN images (group II); 3,000 original photos (group III); and 3,000 original photos + 1,000 GAN images (group IV). The amount of data in each learning group was augmented using existing data augmentation methods such as rotating, cropping, erasing, and distorting. The augmented photo data were used to train the MobileNet_v3_Large, ResNet-152, InceptionResNet_v2, and NASNet_Large frameworks to evaluate the classification accuracy and loss. The top-3 accuracy for each deep neural network model was as follows: MobileNet_v3_Large of 86.4% (group I), 85.4% (group II), 90.4% (group III), and 89.2% (group IV); ResNet-152 of 82.4% (group I), 83.7% (group II), 84.7% (group III), and 84.9% (group IV); InceptionResNet_v2 of 90.7% (group I), 88.4% (group II), 93.3% (group III), and 93.1% (group IV); and NASNet_Large of 85% (group I), 88.1% (group II), 91.8% (group III), and 92% (group IV). The InceptionResNet_v2 model exhibited the highest image classification accuracy, and the NASNet_Large model exhibited the highest increase in the accuracy owing to data augmentation.

• The Journal of the Convergence on Culture Technology
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• v.9 no.1
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• pp.571-580
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• 2023

The purpose of this study is to evaluate the accessibility of the Cakewalk SI-Drum for the screen reader users. An evaluation was performed by testing the accessibility of the [Virtual Instrument Editing] that is a part of MIDI Production of the NCS(National Competency Standards) by using the SI-Drum and the Sense Reader. The results of this study are as follows. The SI-Drum has almost no basic accessibility for the screen users to do an above task. But the screen reader users can perform the tasks by using Sense Reader's Mouse Pointer function and Position Memory function. To use SI-Drum more efficiently, SI-Drum's basic accessibility have to be provided fully. Several suggestions are proposed to develop a fully accessible SI-Drum for the screen users.

• Animal Bioscience
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• v.36 no.6
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• pp.973-979
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• 2023

Objective: The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, which is the most efficient and reliable tool for precisely targeted modification of the genome of living cells, has generated considerable excitement for industrial applications as well as scientific research. In this study, we developed a gene-editing and detection system for chick embryo sexing during the embryonic stage. Methods: By combining the CRISPR/Cas9 technical platform and germ cell-mediated germline transmission, we not only generated Z chromosome-targeted knockin chickens but also developed a detection system for fluorescence-positive male chicks in the embryonic stage. Results: We targeted a green fluorescent protein (GFP) transgene into a specific locus on the Z chromosome of chicken primordial germ cells (PGCs), resulting in the production of Z^GFP-knockin chickens. By mating Z^GFP-knockin females (Z^GFP/W) with wild males (Z/Z) and using a GFP detection system, we could identify chick sex, as the GFP transgene was expressed on the Z chromosome only in male offspring (Z^GFP/Z) even before hatching. Conclusion: Our results demonstrate that the CRISPR/Cas9 technical platform with chicken PGCs facilitates the production of specific genome-edited chickens for basic research as well as practical applications.

• Journal of the Korea Society of Computer and Information
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• v.14 no.1
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• pp.191-203
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• 2009

To provide dynamic services for users in ubiquitous computing environments by considering context in real-time, in our previous work we proposed Metaservice concept, the description specification and the process for building a Metaservice library. However, our previous process generates separated models - UML, OWL, OWL-S based models - from each step, so it did not provide the established method for translation between models. Moreover, it premises aid of experts in various ontology languages, ontology editing tools and the proposed Metaservice specification. In this paper, we design the translation process from domain ontology in OWL to Metaservice Library in OWL-S and develop a visual tool in order to enable non-experts to generate consistent models and to construct a Metaservice library. The purpose of the Metaservice Library translation process is to maintain consistency in all models and to automatically generate OWL-S code for Metaservice library by integrating existing OWL model and Metaservice model.

• Korea Science and Art Forum
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• v.34
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• pp.133-148
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• 2018

Korea's housing structure is predicted that one-person housing will be the most common type of housing in Korea. Therefore, this study intends to derive contents for designing a one-person housing space considering the life of a rapidly increasing one-person householder. For this purpose, this study objectively derives the social, economic and cultural influencing factors of one-person households through big data analysis, and analyzed the correlation between contents using social network analysis methodology. In this paper, 60 core contents related to one person housing space were derived by applying big data analysis methodology. And through social network analysis, the most influential contents were derived from the space editing and space composition categories. This means that the residential space is an important part of the design idea that can flexibly respond to changes in the user's life. Based on this study, future research will focus on the concept and design methodology of one-person housing space.

• Journal of Genetic Medicine
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• v.20 no.1
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• pp.6-14
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• 2023

Fabry disease (FD), a rare X-linked lysosomal storage disorder, is caused by mutations in the α-galactosidase A gene gene encoding α-galactosidase A (α-Gal A). The functional deficiency of α-Gal A results in progressive accumulation of neutral glycosphingolipids, causing multi-organ damages including cardiac, renal, cerebrovascular systems. The current treatment is comprised of enzyme replacement therapy (ERT), oral pharmacological chaperone therapy and adjunctive supportive therapy. ERT has been introduced 20 years ago, changing the outcome of FD patients with proven effectiveness. However, FD patients have many unmet needs. ERT needs a life-long intravenous therapy, inefficient bio-distribution, and generation of anti-drug antibodies. Migalastat, a pharmacological chaperone, augmenting α-Gal A enzyme activity only in patients with mutations amenable to the therapy, is now available for clinical practice. Furthermore, these therapies should be initiated before the organ damage becomes irreversible. Development of novel drugs aim at improving the clinical effectiveness and convenience of therapy. Clinical trial of next generation ERT is underway. Polyethylene glycolylated enzyme has a longer half-life and potentially reduced antigenicity, compared with standard preparations with longer dosing interval. Moss-derived enzyme has a higher affinity for mannose receptors, and seems to have more efficient access to podocytes of kidney which is relatively resistant to reach by conventional ERT. Substrate reduction therapy is currently under clinical trial. Gene therapy has now been started in several clinical trials using in vivo and ex vivo technologies. Early results are emerging. Other strategic approaches at preclinical research level are stem cell-based therapy with genome editing and systemic mRNA therapy.

• Fashion & Textile Research Journal
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• v.25 no.3
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• pp.366-378
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• 2023

Recently, the use of one-person broadcast content for special makeup videos has gained momentum. This study aims to analyze the factors preferred by various age groups as the total number of clicks increase on special makeup videos. It also aims to provide beauty creators with useful data to help them create videos that generate more clicks based on the derived elements. We analyzed previous studies and divided the subjective indicators into place & accessories, editing, model (Y/N), contents and field, and characteristics of the creators. We analyzed top 5 videos from among 257 videos uploaded before July 1, 2020. The subjective indicators were analyzed through a questionnaire survey attempted by 60 respondents in their 10-30s from Jeollanam-do and Gwangju between July 20 to August 31, 2020. The questions majorly focused on intimacy, attractiveness, professionalism, informativity, and playfulness. We analyzed the collected data using SPSS 21.0, and obtained the following results: Informativity, professionalism, attractiveness, and playfulness were considered to be more influential by those aged 10 to 30 years. In particular, factors like visual elements, linguistic characteristics, experts, background music and sound effects, celebrities, product information, and knowhow were most preferred. In fact, it was easy to make videos using these elements. The above results confirmed the utility of such data for beauty-creators-to-be in creating videos that generate more clicks.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.3
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• pp.168-176
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• 2023

Silicon (Si) has the potential to improve plant growth and stress tolerance. The study aimed to explore Si-involving plant responses and molecular characterization of different Si-responsive genes in alfalfa. In this study, the exogenous supplementation of Si enhanced plant growth, and biomass yield. Si-acquisition in alfalfa root and shoot was higher in Si-supplemented compared to silicon deficient (-Si) plants, implying Si-acquisition has beneficial on alfalfa plants. As a consequence, the quantum efficiency of photosystem II (Fv/Fm) was significantly increased in silicon-sufficient (+Si) plants. The quantitative gene expression analysis exhibited a significant upregulation of the Lsi1, Lsi2, Lsi3, NIP5;1, and NIP6;1 genes in alfalfa roots, while BOR1, BOR4, NIP2, and NIP3 showed no significant variation in their expression. The MEME results further noticed the association of four motifs related to the major intrinsic protein (MIP). The interaction analysis revealed that NIP5;1 and Lsi1 showed a shared gene network with NIP2, BOR1, and BOR4, and Lsi2, Lsi3 and NIP3-1, respectively. These results suggest that members of the major intrinsic proteins (MIPs) family especially Lsi1, Lsi2, Lsi3, NIP5;1, and NIP6;1 genes helped to pass water and other neutral solutes through the cell membrane and those played significant roles in Si uptake and transport in plants. Together, these insights might be useful for alfalfa breeding and genome editing approaches for alfalfa improvement.

• The Journal of the Convergence on Culture Technology
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• v.9 no.5
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• pp.591-596
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• 2023

This study developed a program that can produce videos easily and conveniently, focusing on teenage media producers. Through user research, we identified the needs and problems of teenage producers, and implemented a character customization function desired by users and an emotion and action recommendation system using GPT. In the rendering process, the final image was created by combining audio and video using OpenCV and FFmpeg. Teenage users who do not have expertise in video production can customize web drama characters through a simple interface and receive recommendations for emotions and actions with the help of GPT. The program of this study is expected to be a tool that can help teenage users who do not have expertise in editing and directing to produce high-quality videos, lower the entry barrier to video production, and contribute to the development of the one-person media industry. do. In the future, we plan to provide a video production environment considering mobile or vertical resolution versions.

• Journal of Microbiology and Biotechnology
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• v.33 no.9
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• pp.1228-1237
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• 2023

The CRISPR-Cas system has emerged as the most efficient genome editing technique for a wide range of cells. Delivery of the Cas9-sgRNA ribonucleoprotein complex (Cas9 RNP) has gained popularity. The objective of this study was to develop a quantitative polymerase chain reaction (qPCR)-based assay to quantify the double-strand break reaction mediated by Cas9 RNP. To accomplish this, the dextransucrase gene (dsr) from Leuconostoc citreum was selected as the target DNA. The Cas9 protein was produced using recombinant Escherichia coli BL21, and two sgRNAs were synthesized through in vitro transcription to facilitate binding with the dsr gene. Under optimized in vitro conditions, the 2.6 kb dsr DNA was specifically cleaved into 1.1 and 1.5 kb fragments by both Cas9-sgRNA365 and Cas9-sgRNA433. By monitoring changes in dsr concentration using qPCR, the endonuclease activities of the two Cas9 RNPs were measured, and their efficiencies were compared. Specifically, the specific activities of dsr365RNP and dsr433RNP were 28.74 and 34.48 (unit/㎍ RNP), respectively. The versatility of this method was also verified using different target genes, uracil phosphoribosyl transferase (upp) gene, of Bifidobacterium bifidum and specific sgRNAs. The assay method was also utilized to determine the impact of high electrical field on Cas9 RNP activity during an efficient electroporation process. Overall, the results demonstrated that the qPCR-based method is an effective tool for measuring the endonuclease activity of Cas9 RNP.