• Journal of Life Science
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• v.30 no.8
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• pp.701-707
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• 2020

Gene editing technology using the clustered regularly interspaced short palindromic repeat (CRISPR) and the CRISPR associated protein (Cas)9 has been highly anticipated in developing breeding techniques. In this study, we discuss gene scissors as a tool for silkworm molecular breeding through analysis of Bombyx mori Kynurenine 3-Monooxygenase (BmKMO) gene editing using the CRISPR/Cas9 system and analysis of generational transmission through mutagenesis and selective crossing. The nucleotide sequence of the BmKMO gene was analyzed, and three guide RNAs (gRNAs) were prepared. Each synthesized gRNA was combined with Cas9 protein and then analyzed by T7 endonuclease I after introduction into the BM-N silkworm cell line. To edit the silkworm gene, K1P gRNA and Cas9 complexes were subsequently microinjected into the silkworm embryos; the hatching rate was 18% and the incidence of mutation was 60%. The gene mutation was verified in the heterozygous G0 generation, but no phenotypic change was observed. In homozygotes generated by self-crossing, a mutant phenotype was observed. These results suggest that silkworm molecular breeding using the CRISPR/Cas9 system is possible and could be an effective way of shortening the time required.

• Journal of Intelligence and Information Systems
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• v.19 no.4
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• pp.97-121
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• 2013

With the growing importance of knowledge for sustainable competitive advantages and innovation in a volatile environment, many researches on knowledge sharing have been conducted. However, previous researches have mostly relied on the questionnaire survey which has inherent perceptive errors of respondents. The current research has drawn the relationship among primary participant behaviors towards the participation level in knowledge sharing, basically from online user behaviors on Wikipedia, a representative community for online knowledge collaboration. Without users' participation in knowledge sharing, knowledge collaboration for creating knowledge cannot be successful. By the way, the editing patterns of Wikipedia users are diverse, resulting in different revisiting periods for the same number of edits, and thus varying results of shared knowledge. Therefore, we illuminated the participation level of knowledge sharing from two different angles of number of edits and revisiting period. The behavioral dimensions affecting the level of participation in knowledge sharing includes the article talk for public discussion and user talk for private messaging, and community registration, which are observable on Wiki platform. Public discussion is being progressed on article talk pages arranged for exchanging ideas about each article topic. An article talk page is often divided into several sections which mainly address specific type of issues raised during the article development procedure. From the diverse opinions about the relatively trivial things such as what text, link, or images should be added or removed and how they should be restructured to the profound professional insights are shared, negotiated, and improved over the course of discussion. Wikipedia also provides personal user talk pages as a private messaging tool. On these pages, diverse personal messages such as casual greetings, stories about activities on Wikipedia, and ordinary affairs of life are exchanged. If anyone wants to communicate with another person, he or she visits the person's user talk page and leaves a message. Wikipedia articles are assessed according to seven quality grades, of which the featured article level is the highest. The dataset includes participants' behavioral data related with 2,978 articles, which have reached the featured article level, with editing histories of articles, their article talk histories, and user talk histories extracted from user talk pages for each article. The time period for analysis is from the initiation of articles until their promotion to the featured article level. The number of edits represents the total number of participation in the editing of an article, and the revisiting period is the time difference between the first and last edits. At first, the participation levels of each user category classified according to behavioral dimensions have been analyzed and compared. And then, robust regressions have been conducted on the relationships among independent variables reflecting the degree of behavioral characteristics and the dependent variable representing the participation level. Especially, through adopting a motivational theory adequate for online environment in setting up research hypotheses, this work suggests a theoretical framework for the participation level of online knowledge sharing. Consequently, this work reached the following practical behavioral results besides some theoretical implications. First, both public discussion and private messaging positively affect the participation level in knowledge sharing. Second, public discussion exerts greater influence than private messaging on the participation level. Third, a synergy effect of public discussion and private messaging on the number of edits was found, whereas a pretty weak negative interaction effect of them on the revisiting period was observed. Fourth, community registration has a significant impact on the revisiting period, whereas being insignificant on the number of edits. Fifth, when it comes to the relation generated from private messaging, the frequency or depth of relation is shown to be more critical than the scope of relation for the participation level.

• Spatial Information Research
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• v.13 no.3 s.34
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• pp.309-321
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• 2005

To analyze travel demand fur transportation policy and transportation planning, it is important to construct realistic and reliable traffic data. And it needs a user friendly system to demonstrate transportation problems in the transportation planning and transportation management aspect. Generally, to construct network for analysis and collection about social and economical data is a core of transportation planning model. However, it takes a lot of time and effect. To overcome this problem GIS is more effective and efficient in data processing, such as selecting, editing and visualizing, etc. However, it is an early stage to use CIS in the transportation problems. This paper shows a new GIS-T system. The system can give traffic information and plan transportation planning using GIS which has ability as spatial representation and spatial analysis. To build this system, we design interfaces that are able to communicate transportation package for analysis with GIS and manage network efficiently, such as editing and examination. And we also develop a module for traffic information processing to handle spatial data and add it on the system. The proposed system shows more realistic transportation network modeling because the system presents more effective conditions to analyze network. And it can be a tool that can analyze various transportation problems.

• Cartoon and Animation Studies
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• s.14
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• pp.37-51
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• 2008

The computer has become an indispensable tool for animation works. However if you don't understand the characteristics of the computer and its software, you might not have the result satisfying your efforts. The incorrect understanding of image format sometimes causes it. Habitually image formats are selected usually for most of works but there is a distinct difference among those image formats while the efficient usages of them are different from each other. For your more efficient work therefore, you need to identify the characteristics of various kinds of image format used mostly for animation works. First I took a look at the theories of the lossy compression and lossless compression, which are two types of data compression widely used in the whole parts of computer world and the difference between bitmap method and vector method, which are respectably different in terms of the way of expressing images and finally the 24 bit true color and 8 bits alpha channel. Based on those characteristics, I have analyzed the functional difference among image formats used between various types of animation works such as 2D, 3D, composing and editing and also the benefits and weakness of them. Additionally I've proved it is wrong that the JPEG files consume a small space in computer work. In conclusion, I suggest the TIF format as the most efficient format for whatever it is editing, composing, 3D and 2D in considering capacity, function and image quality and also I'd like to recommend PSD format which has compatibility and excellent function, since the Adobe educational programs are used a lot for the school education. I hope this treatise to contribute to your right choice of image format in school education and practical works.

• Journal of the Korean Society for information Management
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• v.19 no.4
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• pp.96-111
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• 2002

A lot of accumulated data of many quantity exist within a institution or an organization, but most data is remained in form of standardization as each institution or organization. There are difficulty in exchange and share of information. New concept of knowledge information resource management to overcome this disadvantage was introduced, and the digitization of knowledge information resources to share and manage accumulated data is been doing. Specially, in science technic or education scholarship it, the tendency that importing XML to process necessary data to exchange and share of knowledge information resources structurally, and limitation of back for search and indexing or reusability is happened according as expression of great many mathematics used inside electron document of these sphere is processed to nonstructural data of image or text and so on. There is interest converged in processing of mathematics that use MathML to overcome this, and we require the solution to be able to process MathML easily and efficiently on structural document. In this paper, designed and implemented of XML document editing system which easy structural process of electronic document for knowledge information resources, and create and express MathML easily on structural document without expert knowledge about MathML.

• Journal of the Korean Association of Geographic Information Studies
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• v.9 no.3
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• pp.25-35
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• 2006

Standardization or standard-related study are regarded as main issues in GIS applications. Though several GIS standards and specifications have been released, there are a few actual application cases adapting those. In this study, we designed and implemented a geo-spatial information processing system with editing, storing, and disseminating functions, in which standard GIS transportation data model by TTA linked with OGC-GML, XML-based geographic features encoding standard. The system developed in this study enables us to transfer and edit transportation entities based on TTA standards to GML, importing ESRI shapefile. In web-based system, GML-based databases are transformed to SVG file, for the purpose of web publishing. TTA GIS transportation data model is used in this study, and tested; however, standard data models from other application fields also can be easily applied because this system basically provides data importing and editing functions. This system as practical tools can be utilized for applicability test of GIS standard data model and practical operation of standard specification.

• Journal of The Korean Association For Science Education
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• v.37 no.2
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• pp.313-322
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• 2017

This study aims to analyze the content and methodological aspects of converging scientific technology and humanities in 233 elementary school STEAM programs developed and publicized from 2012 to 2015, to provide the basic materials and implications for editing and supplementing the development of future STEAM programs or policies, or on-site applications of developed programs. The analysis results show that among the Liberal Arts courses of the STEAM program, including Korean, Social Studies, and Ethics, the level of convergence was lowest in Ethics. This seems to be due to the fact that convergence between scientific technology and humanities tended to emphasize a cross-studies convergence over a content convergence done with a specific purpose. In terms of methodological convergence between scientific technology and humanities, the level of convergence of humanities at a normative level was relatively lower than that at a descriptive level. Although a quantitative comparison of the two methods was not significant, it is rather concerning that due to simply converging descriptive humanities, students may not be so aware of the double-sidedness of scientific technology, despite the discovery of some programs that need re-evaluations of their ethical or normative directions. Moreover, the results showed that the cases of converging scientific technology and humanities at a normative level that were analyzed in the STEAM program can be divided into 'complementation of humanities to utilizing scientific technology' and 'complementation of humanities to research in scientific technology and development.' Based on such research results, the study discusses the development of STEAM programs and policies and directions for editing and supplementing programs on-site.

• Korean journal of communication and information
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• v.26
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• pp.83-104
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• 2004

Most studies of Korean Newspaper in historical view have focused on the news writing form and editorial practice. Those studies have much rely on the memories of ex-journalist or the impression of scholars. So this study aims to give the concrete figures of news writing forms and editing practices in 1920-30's by investigating Kim Dong-Seong's activities as a journalist. He was a first journalist who studies journalism. He studied journalism during his stay in the Ohio State University as an english department student. After he came back to Seoul, he worked at the Dong-A Il Bo as an one of the first publish members. His activities as a journalist have much important meanings because of his varied works and careers. He also wrote a practical affair book for reporters which was the first book in Korea. As a result of research about Kim Dong-Seong's activities in 1920-30's, the feature of edit practice in 1920's had much emphasis not only on the headline but on the relation between type and print, and at the same time the combination of news or the change of typography was one of methods which make the editing more variety. News materials were collected varied news sources and legworks by reporter. These results show us that such a news reporting practice in 1920-30's is similar co the contemporary.

• Journal of the Korea Computer Graphics Society
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• v.20 no.3
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• pp.1-8
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• 2014

We propose a method to compose a foreground object into a background image, where the foreground object is a part (or a region) of an image taken by a front-facing camera and the background image is a whole image taken by a back-facing camera in a smart phone at the same time. Recent high-end cell-phones have two cameras and provide users with preview video before taking photos. We extract the foreground object that is moving along with the front-facing camera using the optical flow during the preview. We compose the extracted foreground object into a background image using a simple image composition technique. For better-looking result in the composed image, we apply a border smoothing technique using a weighted-border mask to blend transparency from background to foreground. Since constructing and grouping pixel-level dense optical flow are quite slow even in high-end cell-phones, we compute a mask to extract the foreground object in low-resolution image, which reduces the computational cost greatly. Experimental result shows the effectiveness of our extraction and composition techniques, with much less computational time in extracting the foreground object and better composition quality compared with Poisson image editing technique which is widely used in image composition. The proposed method can improve limitedly the color bleeding artifacts observed in Poisson image editing using weighted-border blending.

• Molecules and Cells
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• v.41 no.11
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• pp.953-963
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• 2018

The stepwise development of T cells from a multipotent precursor is guided by diverse mechanisms, including interactions among lineage-specific transcription factors (TFs) and epigenetic changes, such as DNA methylation and hydroxymethylation, which play crucial roles in mammalian development and lineage commitment. To elucidate the transcriptional networks and epigenetic mechanisms underlying T-cell lineage commitment, we investigated genome-wide changes in gene expression, DNA methylation and hydroxymethylation among populations representing five successive stages of T-cell development (DN3, DN4, DP, $CD4^+$, and $CD8^+$) by performing RNA-seq, MBD-seq and hMeDIP-seq, respectively. The most significant changes in the transcriptomes and epigenomes occurred during the DN4 to DP transition. During the DP stage, many genes involved in chromatin modification were up-regulated and exhibited dramatic changes in DNA hydroxymethylation. We also observed 436 alternative splicing events, and approximately 57% (252) of these events occurred during the DP stage. Many stage-specific, differentially methylated regions were observed near the stage-specific, differentially expressed genes. The dynamic changes in DNA methylation and hydroxymethylation were associated with the recruitment of stage-specific TFs. We elucidated interactive networks comprising TFs, chromatin modifiers, and DNA methylation and hope that this study provides a framework for the understanding of the molecular networks underlying T-cell lineage commitment.