• 한국인터넷방송통신학회논문지
• /
• 제17권4호
• /
• pp.163-172
• /
• 2017

최근 방송영상분야에서 영상편집 방법으로 비선형편집(Non-Linear Editing; NLE)을 주로 사용하고 있다. 기존의 선형편집에 비해 비선형편집은 컷(Cut)의 삽입과 삭제가 용이하고, 영상편집 시 원하는 위치의 영상에 바로 접근할 수 있다. 또한, 타이틀과 효과, 장면전환 효과를 적용할 수 있고 출력 전에 미리보기를 통해 적용한 타이틀과 효과를 확인하고 수정하는 것이 용이한 장점이 있다. 그러나, NLE편집을 처음 접하는 학생들이 그것을 배우는 것은 쉽지 않다. 본 논문에서는 비선형편집을 처음 접하는 학생들이 쉽게 배울 수 있는 기존의 상호동료교수법(Reciprocal Peer Tutoring; RPT)를 보완한 새로운 RPT 학습모형을 제시한다. 제안하는 교수학습모형을 적용한 실험집단과 적용하지 않는 비교집단으로 나누어 실험을 실시한다. 두 집단의 전체 평균, 성적 하위 집단의 학업 성취도, 표준편차, T검정과 함께 설문조사를 통한 만족도를 실시한다. 제안하는 학습모형을 적용한 실험집단이 통제집단에 비해 지표와 만족도에서 우월함을 보인다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.194-194
• /
• 2022

Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.200-200
• /
• 2022

Recently, food-induced allergies have emerged as major global concerns. In the past ten years, it has doubled in western nations, and it has also increased in Asia and Africa. In many cases of food allergy, peanut allergy is prevalent, typically permanent, and frequently life-threatening. Therefore, we utilized gene editing techniques on the three major allergen genes in peanuts, Ara h 1, Ara h 2, and Ara h 3. Using gibson assembly and golden gate assembly, we created two vectors, the gRNA-tRNA array CRISPR-Cas9 system and Prime-editing. Using LBA4404 strain and agrobacterium-mediated transformation, the vectors were transferred to two elite Korean peanut lines. After co-cultivation and tissue culture, we extracted the tissue cultured peanut DNA amplified the hygromycin resistance gene and Cas9 gene in the T-DNA region. The integration of the T-DNA region into the host genome was demonstrated by the presence of a specific band in some samples. There have only been a few reported peanut gene editing studies. So, this study will contribute to peanut allergy and gene editing research.

• BMB Reports
• /
• 제57권1호
• /
• pp.19-29
• /
• 2024

Mitochondrial DNA (mtDNA), a multicopy genome found in mitochondria, is crucial for oxidative phosphorylation. Mutations in mtDNA can lead to severe mitochondrial dysfunction in tissues and organs with high energy demand. MtDNA mutations are closely associated with mitochondrial and age-related disease. To better understand the functional role of mtDNA and work toward developing therapeutics, it is essential to advance technology that is capable of manipulating the mitochondrial genome. This review discusses ongoing efforts in mitochondrial genome editing with mtDNA nucleases and base editors, including the tools, delivery strategies, and applications. Future advances in mitochondrial genome editing to address challenges regarding their efficiency and specificity can achieve the promise of therapeutic genome editing.

• International Journal of Stem Cells
• /
• 제17권1호
• /
• pp.1-14
• /
• 2024

The clustered regularly interspaced short palindromic repeats (CRISPR) system, a rapidly advancing genome editing technology, allows DNA alterations into the genome of organisms. Gene editing using the CRISPR system enables more precise and diverse editing, such as single nucleotide conversion, precise knock-in of target sequences or genes, chromosomal rearrangement, or gene disruption by simple cutting. Moreover, CRISPR systems comprising transcriptional activators/repressors can be used for epigenetic regulation without DNA damage. Stem cell DNA engineering based on gene editing tools has enormous potential to provide clues regarding the pathogenesis of diseases and to study the mechanisms and treatments of incurable diseases. Here, we review the latest trends in stem cell research using various CRISPR/Cas technologies and discuss their future prospects in treating various diseases.

• 공학논문집
• /
• 제3권1호
• /
• pp.21-27
• /
• 1998

본 논문에서는 SGML DTD(Document Type Definition)의 문서 구조정보를 이용하여 SGML 실례문서를 편집하기 위한 시스템을 설계 및 구현하였다. 이를 위해 문서의 논리구조 표현을 위한 구조 창을 이용하여 SGML 문서를 편집할 수 있어 SGML에 대해 모르는 사용자도 편집오류 없이 문서를 생성할 수 있고 엘리먼트(element)와 속성(attribute), 엔티티(entity)를 지원하는 도구를 이용하여 엘리먼트 등을 손쉽게 수정 가능하고, 생성된 문서를 SGML 파서(parser)를 이용하여 검증할 수 있도록 시스템을 설계하였다. 또한 본 시스템은KS 5601코드를 사용하여 한글과 영문 텍스트를 모두 지원한다. 본 논문에서 설계한 SGML 문서 편집 시스템은 윈도우 사용자 인터페이스를 위해 윈도우95 시스템 환경 하에서 구현하였다.

• Molecules and Cells
• /
• 제41권10호
• /
• pp.917-922
• /
• 2018

The CRISPR-Cas system is a well-established RNA-guided DNA editing technique widely used to modify genomic DNA sequences. I used the CRISPR-Cas9 system to change the second and third nucleotides of the triplet $T{\underline{CT}}$ of human TNSFSF9 in HepG2 cells to $T{\underline{AG}}$ to create an amber stop codon. The $T{\underline{CT}}$ triplet is the codon for Ser at the $172^{nd}$ position of TNSFSF9. The two substituted nucleotides, AG, were confirmed by DNA sequencing of the PCR product followed by PCR amplification of the genomic TNFSF9 gene. Interestingly, sequencing of the cDNA of transcripts of the edited TNFSF9 gene revealed that the $T{\underline{AG}}$ had been re-edited to the wild type triplet $T{\underline{CT}}$, and 1 or 2 bases just before the triplet had been deleted. These observations indicate that CRISPR-Cas9-mediated editing of bases in target genomic DNA can be followed by spontaneous re-editing (correcting) of the bases during transcription.

• 생명과학회지
• /
• 제8권2호
• /
• pp.203-207
• /
• 1998

본 연구는 옥수수에서 분리한 미토콘드리아에서 NADH-dehydrogenase 유전자 (subunit 4)의 cDNA를 RT-PCR의 방법을 사용하여 조제 한 ㅜ 염기서열 수행한 경과 특이한 점을 감지 할 수 있었다. 일반적인 RNA cditing은 C에서 U로 또는 U에서 C로 치환되는 현장으로 옥수수의 NAD4유전자에서도 이러한 editing 형상이 일어나는 것을 발견하였다. 또는 T가 G로 그리고 G 가 A로 변화되는 특이한 부분들이 생성되는 것을 관찰하였다. 이러한 RNA ediring은 주로 exon 1과 exon 4 에 많이 일어나며, 염기 치환되는 부분들은 에서늬 NAD4유전자의 RNA edting site들과 일피하지 않은 점으로 미루어 보아 RNA editing 현상은 무작의로 생성된다고 본다.된다고 본다.

• Bulletin of the Korean Chemical Society
• /
• 제28권2호
• /
• pp.207-210
• /
• 2007

To identify structural or functional common subunit(s) in the CP1 (editing) domains of class Ia tRNA synthetases, five available structures were compared and analyzed. Through the sequence alignments and structural overlapping of the CP1 domains, three conserved regions were identified near the amino acid binding site in the editing domain. Structural overlapping of the three subunits clearly showed the existence of three common structural subunits in all of the five editing RS structures. Based on the established experimental results and our modeling results, it is proposed that subunits 1 and 3 accommodate the incoming amino acid binding, while subunit 2 contributes to the interactions with the adenosine ring of the A76 to stabilize the overall tRNA binding. Since these subunits are critical for the editing reaction, we expect that these key structures should be conserved through the most class Ia editing RSs.

• Molecules and Cells
• /
• 제44권12호
• /
• pp.911-919
• /
• 2021

The virus-induced genome editing (VIGE) system aims to induce targeted mutations in seeds without requiring any tissue culture. Here, we show that tobacco rattle virus (TRV) harboring guide RNA (gRNA) edits germ cells in a wild tobacco, Nicotiana attenuata, that expresses Streptococcus pyogenes Cas9 (SpCas9). We first generated N. attenuata transgenic plants expressing SpCas9 under the control of 35S promoter and infected rosette leaves with TRV carrying gRNA. Gene-edited seeds were not found in the progeny of the infected N. attenuata. Next, the N. attenuata ribosomal protein S5 A (RPS5A) promoter fused to SpCas9 was employed to induce the heritable gene editing with TRV. The RPS5A promoter-driven SpCas9 successfully produced monoallelic mutations at three target genes in N. attenuata seeds with TRV-delivered guide RNA. These monoallelic mutations were found in 2%-6% seeds among M₁ progenies. This editing method provides an alternative way to increase the heritable editing efficacy of VIGE.