• Journal of the Korean Society of Earth Science Education
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• v.13 no.3
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• pp.211-225
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• 2020

The purpose of this study was to investigate and analyze pre-service teachers' eye-movement in science textbooks in the learning process, whether they perceived "arrow" presented in textbooks, and changes in eye-movement by arrows. For the study, an eye-tracker, a eye-movement tracking device, was used, and 10 pre-service teachers attending a teacher training college were selected and conducted. The science textbook unit used in the research was the "Weather and Our Life" unit, one of the areas of earth science. As a result of the study, first, it was investigated that pre-service teachers devote more time to texts rather than illustrations in the learning process of science textbooks. Second, it was analyzed that pre-service teachers did not pay attention to the "arrow" presented in science textbooks. Third, it was confirmed that in order for "affordance of the eye-movement by arrow" to occur, sufficient concentration on "arrow" should be made. These findings suggest the importance of the learner's visual attention to learning elements in science textbooks such as "arrow". In addition, it suggests the importance of developing a textbook editing design that can induce visual attention to learning elements in textbooks through eye-movement research data for effective learning.

• Biomolecules & Therapeutics
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• v.29 no.5
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• pp.551-561
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• 2021

Thyroid cancer is the most common endocrine malignancy. Patients with well-differentiated thyroid cancers, such as papillary and follicular cancers, have a favorable prognosis. However, poorly differentiated thyroid cancers, such as medullary, squamous and anaplastic advanced thyroid cancers, are very aggressive and insensitive to radioiodine treatment. Thus, novel therapies that attenuate metastasis are urgently needed. We found that both PDGFC and PDGFRA are predominantly expressed in thyroid cancers and that the survival rate is significantly lower in patients with high PDGFRA expression. This finding indicates the important role of PDGF/PDGFR signaling in thyroid cancer development. Next, we established a SW579 squamous thyroid cancer cell line with 95.6% PDGFRA gene insertion and deletions (indels) through CRISPR/Cas9. Protein and invasion analysis showed a dramatic loss in EMT marker expression and metastatic ability. Furthermore, xenograft tumors derived from PDGFRA geneedited SW579 cells exhibited a minor decrease in tumor growth. However, distant lung metastasis was completely abolished upon PDGFRA gene editing, implying that PDGFRA could be an effective target to inhibit distant metastasis in advanced thyroid cancers. To translate this finding to the clinic, we used the most relevant multikinase inhibitor, imatinib, to inhibit PDGFRA signaling. The results showed that imatinib significantly suppressed cell growth, induced cell cycle arrest and cell death in SW579 cells. Our developed noninvasive apoptosis detection sensor (NIADS) indicated that imatinib induced cell apoptosis through caspase-3 activation. In conclusion, we believe that developing a specific and selective targeted therapy for PDGFRA would effectively suppress PDGFRA-mediated cancer aggressiveness in advanced thyroid cancers.

• The Journal of the Convergence on Culture Technology
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• v.8 no.1
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• pp.239-244
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• 2022

Neural Machine Translation (NMT) is gradually breaking down the boundary between human and machine translation. We look at actual cases of human and machine translation and discuss why machine translation needs a human touch. In this paper, we raise three driving questions: Can humans be replaced by machines?; How human translators can remain successful in a NMT-driven world?; Is it possible to eliminate language barrier in the era of NMT and World Englishes? The answers to these questions are all negative. We suggest that machine translation is a useful tool with rapidity, accuracy, and low cost productivity. However, the machine translation is limited in the areas of culture, borrowing, ambiguity, new words and (national) dialects. The machines cannot imitate the emotional and intellectual abilities of human translators since machines are based on machine learning, while humans are on intuition. The machine translation will be a useful tool that does not cause moral problems when using methods such as back translation and human post-editing. To conclude, we propose the blended approach that machine translation cannot be completed without the touch of human translation.

• Animal Bioscience
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• v.35 no.1
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• pp.126-137
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• 2022

Objective: Efficient gene editing technology is critical for successful knock-in in domestic animals. RAD51 recombinase (RAD51) gene plays an important role in strand invasion during homologous recombination (HR) in mammals, and is regulated by checkpoint kinase 1 (CHK1) and CHK2 genes, which are upstream elements of RAD51 recombinase (RAD51). In addition, mismatch repair (MMR) system is inextricably linked to HR-related pathways and regulates HR via heteroduplex rejection. Thus, the aim of this study was to investigate whether clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9)-mediated knock-in efficiency of human lactoferrin (hLF) knock-in vector in the bovine β-casein gene locus can be increased by suppressing DNA MMR-related genes (MSH2, MSH3, MSH6, MLH1, and PMS2) and overexpressing DNA double-strand break (DSB) repair-related genes (RAD51, CHK1, CHK2). Methods: Bovine mammary epithelial (MAC-T) cells were transfected with a knock-in vector, RAD51, CHK1, or CHK2 overexpression vector and CRISPR/sgRNA expression vector to target the bovine β-casein gene locus, followed by treatment of the cells with CdCl₂ for 24 hours. After 3 days of CdCl₂ treatment, the knock-in efficiency was confirmed by polymerase chain reaction (PCR). The mRNA expression levels of DNA MMR-related and DNA DSB repair-related genes were assessed by quantitative real-time PCR (RT-qPCR). Results: Treatment with CdCl₂ decreased the mRNA expression of RAD51 and MMRrelated genes but did not increase the knock-in efficiency in MAC-T cells. Also, the overexpression of DNA DSB repair-related genes in MAC-T cells did not significantly affect the mRNA expression of MMR-related genes and failed to increase the knock-in efficiency. Conclusion: Treatment with CdCl₂ inhibited the mRNA levels of RAD51 and DNA MMR-related genes in MAC-T cells. However, the function of MMR pathway in relation to HR may differ in various cell types or species.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.415-420
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• 2021

In a situation where the medical care field is developing from various angles, medical promotion video analysis has an important meaning. It is important as a matter of improving competitiveness, and in the era of acceleration of AI systems, medical care is also the leading field. Accordingly, the importance of videos on publicity, advertisements, and explanations is very important, and it is also an important direction to change the image of a company. In this study, the design characteristics and differences in the video were compared, focusing on the comparative analysis of professional videos of AI medical brands, with two foreign major companies (Stryker and Hill-rom) and one domestic leading company (Nine Bell), and detailed part analysis and section analysis were performed accordingly. As a technical partial analysis of image editing, the transition method and infographic graphics were considered. In an in-depth comparison, we found that AI medical imaging Points such as differences in image tone and image color harmony were analyzed and compared. For a detailed analysis in the video image determination part, we compared and studied the differentiated elements appearing in the promotional design and specific scenes of the video intro part and the product description video part of each video.

• Journal of Korea Entertainment Industry Association
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• v.15 no.4
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• pp.73-87
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• 2021

Special theaters were created to provide a sense of immersion and spectacles due to differentiated screens, sound, seating facilities, and advanced services, and also expanded screens. The purpose of this study is to perform comparative analysis of the technical characteristics formats shown in special theaters(3D film, 4DX, IMAX, ScreenX, and VR) in order to identify and find ways to overcome the technological limitations in production. The various formats show differences in field of view depending on the exhibition technology and these differences affect the mise-en-scene, narrative, and editing of the film and consequently result in changes in the production environment and process. Therefore, directors and creators must understand the technological features and limitations of the new formats before making their approach. However, a new format encounters limitations on production sets due to the decline of technical education and succession. In situations where shooting with a special camera is essential, the particular characteristics of each format should be carefully considered from the planning stage but financial problems arise due to increase in production period and cost. To overcome these various obstacles, it is essential to first identify problems and present alternatives through in-depth research on the production set of each format. Finally, this research aims to explore the prototype of each format and analyze the current state of production technology with formats that have not been adapted to the market trends by combining with the other formats and showing that they can survive in new ways.

• Journal of Korea Entertainment Industry Association
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• v.15 no.6
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• pp.1-13
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• 2021

This study explored the mechanism by which Trot gained a superior position in the broadcasting contents market after the TV Chosun audition program was broadcast. We analyzed the narrative structure of the program to determine what differentiation and popularization strategy the trot audition program took from the existing audition program, and analyzed in-depth interviews with music experts and interviews with the production team. appealed to viewers with a strategy that reversed the success strategy of existing audition programs. First, the strong/non-competent participants did not compete with each other, but rather the strong/skilled players competed against each other. This trot audition set the singing ability as a new 'discourse on sincerity'. Second, we broke away from the 'demon editing', which was considered essential for audition programs, took a strategy of excluding villains. Third, we broke the practice of audition programs that were supposed to show expertise in specific genres, such as idol music, hip-hop, and bands, and combined trot with various genres. Fourth, unlike previous audition programs that mainly targeted specific generations or genders, the strategy was to expand the audience by targeting various age groups. Fifth, it has formed a middle-aged fandom with a 'subtitle strategy' that uses subtitles well to arouse viewers' interest and help empathize.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.39 no.6
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• pp.483-490
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• 2021

Due to the recent increase in various problems from underground development in urbanized areas, accurate underground facility information management is highly needed. Therefore, in this study, in order to utilize the Integration Map of Underground Goespatial Information in real time on-site, the function of comparing the mutual location of the GPR (Ground Penetration Radar) sensing data and the Integration Map of Underground Goespatial Information, and function of analyze underground facilities, and function of converting surveying data into a shape file through position correction & attribute editing in a 3D space, and the function of submitting the shape file to the Integration Map of Underground Goespatial Information mobile center was defined and developed as a program. In addition, for the on-site application test of the development program, scenarios used at the underground facility real-time survey site and GPR exploration site were derived, and four sites in Seoul were tested to confirm that the use scenario worked properly. Through this, the on-site utilization of the program developed in this study could be confirmed, and it would contribute to the confirmation of the quality of Shape-file and the "update automation" of "Integration Map of Underground Goespatial Information". In addition, it is expected that the development program will be further applied to the Underground Facility Map's Accuracy Improvement Diffusion Project' promoted by the MOLIT (Ministry of Land, Infrastructure, and Transport).

• Education of Primary School Mathematics
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• v.25 no.4
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• pp.411-431
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• 2022

In this paper, implications for future curriculum compilation were sought by analyzing the process and results of compiling books for elementary school mathematics textbooks government published according to the 2015 revised curriculum. The 2015 revised elementary mathematics textbooks government published was operated with a systematic compilation system so that academia and school field experts across the country could demonstrate their expertise. As improvements in content, the unit and time to strengthen basic computational skills were increased, and the mathematical concept and principle introduction method and algorithm presentation method were improved, and the internal connection between contents was strengthened. The learning period was adjusted, such as moving and arranging contents that are difficult for students to understand to the upper semester or the upper grade. In the 1st and 2nd graders, the amount of reading was drastically reduced to suit the students' level of Korean, and sentences and vocabulary were improved, and instructions were briefly revised. As for editing and design improvements, illustrations of each unit's introduction and contextual pictures were presented in detail, and the characters in the textbook were consistently presented across all grades, giving children characters a role to actively participate in learning in the textbook. In the process of compiling, the media, the National Assembly, and civic groups raised opinions that sentences and vocabulary in first-year textbooks are more difficult than students' level of Hangeul education, that reducing textbooks makes it difficult for students to understand. Accordingly, efforts to improve textbook compilation and the results were viewed. Through the overall analysis as above, for future compilation of state-authored textbooks and certified textbooks, a plan to improve textbook compilation for students and teachers and a plan to operate compilation was proposed.

• Molecules and Cells
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• v.45 no.10
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• pp.718-728
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• 2022

Splicing factor B subunit 4 (SF3B4), a component of the U2-pre-mRNA spliceosomal complex, contributes to tumorigenesis in several types of tumors. However, the oncogenic potential of SF3B4 in lung cancer has not yet been determined. The in vivo expression profiles of SF3B4 in non-small cell lung cancer (NSCLC) from publicly available data revealed a significant increase in SF3B4 expression in tumor tissues compared to that in normal tissues. The impact of SF3B4 deletion on the growth of NSCLC cells was determined using a siRNA strategy in A549 lung adenocarcinoma cells. SF3B4 silencing resulted in marked retardation of the A549 cell proliferation, accompanied by the accumulation of cells at the G0/G1 phase and increased expression of p27, p21, and p53. Double knockdown of SF3B4 and p53 resulted in the restoration of p21 expression and partial recovery of cell proliferation, indicating that the p53/p21 axis is involved, at least in part, in the SF3B4-mediated regulation of A549 cell proliferation. We also provided ubiquitination factor E4B (UBE4B) is essential for p53 accumulation after SF3B4 depletion based on followings. First, co-immunoprecipitation showed that SF3B4 interacts with UBE4B. Furthermore, UBE4B levels were decreased by SF3B4 depletion. UBE4B depletion, in turn, reproduced the outcome of SF3B4 depletion, including reduction of polyubiquitinated p53 levels, subsequent induction of p53/p21 and p27, and proliferation retardation. Collectively, our findings indicate the important role of SF3B4 in the regulation of A549 cell proliferation through the UBE4B/p53/p21 axis and p27, implicating the therapeutic strategies for NSCLC targeting SF3B4 and UBE4B.