• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.16 no.1
• /
• pp.227-244
• /
• 2013

The vascular plants in the trail from Jangansan to Palgongsan were listed 389 taxa (8.0% of all 4,881 taxa of Korean vascular plants); 84 families, 226 genera, 325 species, 1 subspecies, 55 varieties and 8 forms. Furthermore, hemicryptophytes (H) were 110 taxa (28.3%), geophytes (G) were 77 taxa (19.8%), megaphanerophytes (MM) were 48 taxa (12.3%), nanophanerophytes (N) were 47 taxa (12.1%) showed high proportional ratio in life form. So, resource plants of 237 taxa of edible (60.9%), 278 taxa of medicinal (71.5%), 229 taxa of ornamental (58.9%) and 178 taxa of the others (45.8%). Based on the list of rare plants, 7 taxa (1.8% of all 389 taxa of flora and 1.2% of all 571 taxa of rare plants); Paeonia japonica (VU), Viola albida, Viola diamantica, Lilium distichum, Disporum ovale, Tricyrtis dilatata, Iris ensata var. spontanea (LC), etc. Based on the list of endemic plants, 7 taxa (1.8% of all 389 taxa of flora and 2.1% of all 328 taxa of endemic plants); Salix koriyanagi, Aconitum pseudolaeve var. erectum, Thalictrum actaefolium var. brevistylum, Philadelphus schrenckii, Asperula lasiantha, Weigela subsessilis, Carex okamotoi, etc. Based on the list of specific plants, 46 taxa (11.8% of all 389 taxa of flora and 4.3% of all 1,071 taxa of specific plants); 2 taxa (Wisteria floribunda for. floribunda, Cardamine yezoensis, etc.) in class IV, 6 taxa (Acer palmatum, Ulmus davidiana, etc.) in class III, 14 taxa (Ligularia fischeri, Cymopterus melanotilingia, etc.) in class II, 24 taxa (Ilex macropoda, Fraxinus mandshurica, etc.) in class I. Based on the list of naturalized plants, 6 families, 16 genera, 18 taxa (Phytolacca americana, Robinia pseudoacacia, Oenothera odorata, Erechtites hieracifolia, Festuca arundinacea, etc.) and invasive alien plants were 3 taxa; Rumex acetocella, Ambrosia artemisiifolia, Aster pilosus. Naturalization rate (NR) was 4.6% of all 389 taxa of vascular plants and urbanization index (UI) was 5.6% of all 321 taxa of naturalized plants.

• Mycobiology
• /
• v.43 no.3
• /
• pp.327-332
• /
• 2015

Phenylalanine ammonia-lyase (PAL) gene is known to be expressed in plants, and is involved in the differentiation, growth and synthesis of secondary metabolites. However, its expression in fungi remains to be explored. To understand its expression in mushroom fungi, the PAL gene of the edible mushroom Flammulina velutipes (Fvpal) was cloned and characterized. The cloned Fvpal consists of 2,175 bp, coding for a polypeptide containing 724 amino acids and having 11 introns. The translated amino acid sequence of Fvpal shares a high identity (66%) with that of ectomycorrhizal fungus Tricholoma matsutake. Distinctively, the Fvpal expression in the mycelium was higher in minimal medium supplemented with L-tyrosine than with other aromatic amino acids. During cultivation of the mushroom on sawdust medium, Fvpal expression in the fruit body correspondingly increased as the mushroom grew. In the fruiting body, Fvpal was expressed more in the stipe than in the pileus. These results suggest that F. velutipes PAL activity differs in the different organs of the mushroom. Overall, this is first report to show that the PAL gene expression is associated with mushroom growth in fungi.

• Korean Journal of Plant Resources
• /
• v.21 no.3
• /
• pp.196-203
• /
• 2008

Phytotoxicity, antioxidant activity, and cytotoxicity of the aqueous or methanol extracts from the young sprouts of the six Compositae medicinal plants were determined. Aqueous leachates at 40g dry tissue $L^{-1}$ (g $L^{-1}$) Cirsium japonicum and Aster yomena showed the highest inhibitory effect on alfalfa (Medicago sativa L.). Total phenolic content showed the highest amount in methanol extracts from Ixeris dentata, and followed by A. yomena, and Cephalonoplos segetum. Methanol extracts of C. segetum and I. dentata at 25${\mu}g$ m$L^{-1}$ exhibited the highest DPPH radical scavenging activity by 87.2, and 52.8%, respectively. By means of HPLC analysis, MeOH extracts of C. segetum had the highest amount of antioxidant chlorogenic acid. Based on MTT assay, the methanol extracts from Y. sonchifolia ($IC_{50}$ = 65.7${\mu}g$ $mL^{-1}$) showed the highest cytotoxicity against Calu-6. These results suggest that plant extracts had a dose-dependent biological potentials including phytotoxicity, antioxidant activity, and anticancer activity, and that their activities exhibited differently depending on plant species.

• Food Science and Biotechnology
• /
• v.17 no.5
• /
• pp.1097-1101
• /
• 2008

The objective of this study was to identify and quantify glucosinolates (GSLs) in Brassica napus cv. Hanakkori and its parents and to evaluate its potential bitter taste. 'Hanakkori' materials were cultivated with commercial chemical nutrients (20 kg/ha, N-P-K: 16-10-10) at the field. GSLs were isolated by means of extraction with 70%(v/v) boiling methanol (MeOH) followed by desulfation from those plants by reversed-phase high performance liquid chromatography (HPLC) and identified by electronic spray ionization-mass spectrometry (ESI-MS) analysis. In 'Hanakkori', 11 GSLs were identified as progoitrin, glucoraphanin, glucoalyssin, gluconapoleiferin, gluconapin, 1-methylpropyl, glucobrassicanapin, glucobrassicin, 4-methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin. The total GSL contents were 109 and 36.1 mmol/kg dry weights (d.w.) for the seeds and edible parts, respectively. The major GSLs (>5 mmol/kg d.w.) in the seeds were progoitrin (78.8), gluconapin (10.7), and glucobrassicanapin (7.81), whereas they in the edible parts were progoitrin (16.1) and glucobrassicanapin (8.58). In addition, the bitter taste in the edible parts was presumably related with the presence of progoitrin (>45% to the total GSL).

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.226-226
• /
• 2017

This study was to evaluate methods to high quality food ramie rice cake, thereby increasing farm income. This study investigated the effects of different covering material on stable winter survival management with edible leaf in Ramie(Boehmeria nivea L.). The method of winter survival with covering material were conducted under three condition compose to Non covering, Rice straw cutting covered with 500kg.10a-1, Rice husks covered with 1,000kg.10a-1(covered 4~5 cm thickness in the soil surface). Method of application were standard application(N-P-K-Compost applied at 27-9-27-600kg.10a-1. Compost and fused phosphate applied at 100% of basal fertilizer in March 25. 20% of top dressing were four times application in March 25 - October 5. Planting year were March 15, 2011. Plants were spaced 60 cm apart in rows 25 cm apart with open cultivation. According to non covering < Rice husks covered with 1,000kg.10a-1 < Rice straw cutting covered with 500kg.10a-1 cultivation this order, aerial part as a result were plenty amount of growth. Sprout time and winter survival rates was uncovering control plot compared to 2 - 5 days quickly, 45-57% highly by rice husks and rice straw covering. Green leaf yields is untreated control plot (12,44 kg.10a-1) compared to rice husks covering 7% higher, and rice straw covering increased to 18% of the most.

• Mycobiology
• /
• v.28 no.1
• /
• pp.27-32
• /
• 2000

The ectomycorrhizal roots were collected from the soils around the bases of basidiocarps of the four edible mushrooms in the stands of Pinus densiflora or Querus acutissima communities (Mt. Wol-Ak in Eastern Chung-Puk): The basidiocarps of Tricholoma matsutake (TM), Sarcodon asparatus (SA), S. imbricatum (SI), and Polyozellus multiplex (PM) are usually collected. The ectomycorrhizal roots of TM, PM and SI were related to the roots of P. densiflora, but the other to the roots of Q. acutissima in Korea. Particularly, the basidiocarps of PM were collected in the mixed stand of both P. densiflora and Q. acutissima. The morphologies of the ectomycorrhizal roots were observed to be the yellowish brown coral (dichromatous) or pyramid types in the roots of the pine, but dark brown un-branched sticks (roots) in the ends of ectomycorrhizal roots of Querus plants. The un-branched roots were covered with the dark mycelia (rhizomorph) around them. Therefore, the ectomyorrhizal roots of PM were observed to have two kinds of types; The single blackish un-branched roots were observed to be attached to the yellowish coral type roots. The bundles of TM mycelia were filled with cortical cells (in the roots of P. densiflora), but the mycelia of the other fungi (Aphylloporales) were massed between the cortical cells of P. densiflora or Q. acutissima. Their anatomical and gross features were considered to be simailar but very important in the ectomycorrhizal roots for these edible mushrooms.

• 한국균학회소식:학술대회논문집
• /
• 2014.10a
• /
• pp.19-19
• /
• 2014

Pleurotus ostreatus, P. eryngii, and Flammulina velutipes are major edible mushrooms that account for over 89% of total mushroom production in Korea. Recently, Agrocybe cylindracea, Hypsizygus marmoreus, and Hericium erinaceu are increasingly being cultivated in mushroom farms. In Korea, the production of edible mushrooms was estimated to be 614,224 ton in 2013. Generally, about 5 kg of mushroom substrate is needed to produce 1 kg of mushroom, and consequently about 25 million tons of spent mushroom substrate (SMS) is produced each year in Korea. Because this massive amount of SMC is unsuitable for reuse in mushroom production, it is either used as garden fertilizer or deposited in landfills, which pollutes the environment. It is reasonably assumed that SMS includes different secondary metabolites and extracellular enzymes produced from mycelia on substrate. Three major groups of enzymes such as cellulases, xylanases, and lignin degrading enzymes are involved in breaking down mushroom substrates. Cellulase and xylanase have been used as the industrial enzymes involving the saccharification of biomass to produce biofuel. In addition, lignin degrading enzymes such as laccases have been used to decolorize the industrial synthetic dyes and remove environmental pollutions such as phenolic compounds. Basidiomycetes produce a large number of biologically active compounds that show antibacterial, antifungal, antiviral, cytotoxic or hallucinogenic activities. However, most previous researches have focused on therapeutics and less on the control of plant diseases. SMS can be considered as an easily available source of active compounds to protect plants from fungal and bacterial infections, helping alleviate the waste disposal problem in the mushroom industry and creating an environmentally friendly method to reduce plant pathogens. We describe extraction of lignocellulytic enzymes and antimicrobial substance from SMSs of different edible mushrooms and their potential applications.

• Korean Journal of Food Science and Technology
• /
• v.26 no.4
• /
• pp.359-364
• /
• 1994

Volatile flavor components from various edible portions of Calystegia japonica $(T_{HUNB})\;C_{HOIS}$ were collected by simultaneous steam distillation-extraction(SDE) method using diethyl ether as solvent. Essential oils were analyzed by gas chromatography(GC) and combined gas chromatography-mass spectrometry(GC-MS). Thirty nine volatile flavor components, including 21 hydrocarbons, 1 aldehyde, 4 ketones, 7 alcohols, 4 esters, 1 acid and 1 miscellaneous one were confirmed in leaves. Twenty six components, including 16 hydrocarbons, 2 aldehydes, 3 ketones and 5 alcohols were confirmed in stems, and 52 components, including 26 hydrocarbons, 2 aldehydes, 5 ketones, 13 alcohols, 1 ester, 1 acid and 4 miscellaneous ones were confirmed in roots. The kinds and amounts of volatile flavor components revealed different patterns depending upon various edible portions. Relatively greater numbers of volatile flavor components were identified in roots compared with other portions of this wild plant.

• The Journal of the Convergence on Culture Technology
• /
• v.9 no.3
• /
• pp.601-606
• /
• 2023

The edible sweetener aspartame is formed from the synthesis of asparagine, one of the amino acids, and phenylalanine. It is a protein ingredient that produces sweetness 200 times higher than sucrose, and is used as a diet product because it is a substance that can replace sugar. Although the effects on animals and humans have been well studied, no studies have been conducted on plant metabolism. In this study, the effect of aspartame metabolism was tested using germinated onion, bean sprouts, and Kalanchoe. The three types of plants with germinated roots showed rapid growth in the early stage of treatment with 1mM aspartame, and showed a nutrient supply effect due to the effect of amino acids, which are constituents. However, when treated for 4 weeks, symptoms similar to lateral roots appear in the form of lignification in onions, the effect of quickly withering is seen in bean sprouts, and senescence of roots appears in 12-week-old Kalanchoe. All of them show accelerated aging after the growth phase. This shows that, among the two amino acids, asparagine is related to auxin generation that induces rapid growth, and it is thought to be the result of lignification due to the effect of phenylalanine.

• Korean Journal of Plant Resources
• /
• v.29 no.2
• /
• pp.204-224
• /
• 2016

This study was carried out to investigate the vascular plants of the protected area for forest genetic resource conservation in the Korea national Baekdudaegan arboretum. The vascular plants collected 12 times (from April 2007 to August 2014) were consisted of total 569 taxa; 89 families, 289 genera, 489 species, 5 subspecies, 66 varieties and 9 forms respectively. The 20 taxa of Korean endemic plants and 21 taxa of Korean rare plants were investigated. The floristic regional indicator plants found in this area were 112 taxa comprising 2 taxa of grade V, 8 taxa of grade IV, 23 taxa of grade III, 40 taxa of grade II, 39 taxa of grade I. The introduced and naturalized plants were counted as 22 taxa, therefore naturalized ratio was 3.8%. 577 taxa listed consists of 223 taxa (39.2%) of edible plants, 168 taxa (29.5%) of medicinal plants, 202 taxa (35.5%) of pasture plants, 58 taxa (10.2%) of ornamental plants, 26 taxa (4.6%) of timber plants, 5 taxa (0.9%) of fiber plants and 4 taxa (0.7%) of industrial plants.