• Journal of Microbiology and Biotechnology
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• 제28권5호
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• pp.796-808
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• 2018

The intracellular bacterium Wolbachia pipientis is widespread in arthropods. Recently, possibilities of novel Wolbachia-mediated hosts, their distribution, and natural rate have been anticipated, and the coconut leaf beetle Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae), which has garnered attention as a serious pest of palms, was subjected to this interrogation. By adopting Wolbachia surface protein (wsp) and multilocus sequence type (MLST) genotypic systems, we determined the Wolbachia infection density within host developmental stages, body parts, and tissues, and the results revealed that all the tested samples of B. longissima were infected with the same Wolbachia strain (wLog), suggesting complete vertical transmission. The MLST profile elucidated two new alleles (ftsZ-234 and coxA-266) that define a new sequence type (ST-483), which indicates the particular genotypic association of B. longissima and Wolbachia. The quantitative real-time PCR analysis revealed a higher infection density in the eggs and adult stage, followed by the abdomen and reproductive tissues, respectively. However, no significant differences were observed in the infection density between sexes. Moreover, the wsp and concatenated MLST alignment analysis of this study with other known Wolbachia-mediated arthropods revealed similar clustering with distinct monophyletic supergroup B. This is the first comprehensive report on the prevalence, infection dynamics, and phylogeny of the Wolbachia endosymbiont in B. longissima, which demonstrated that Wolbachia is ubiquitous across all developmental stages and distributed in the entire body of B. longissima. Understanding the Wolbachia infection dynamics would provide useful insight to build a framework for future investigations, understand its impacts on host physiology, and exploit it as a potential biocontrol agent.

• 한국환경과학회지
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• 제31권5호
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• pp.389-404
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• 2022

The purpose of this study is to evaluate the vegetation recovery status of Mudeungsan National Park Jungmeorijae, Jeungsimsa district restoration site, and the Shimwon Valley ecological landscape restoration site in Jirisan National Park. Compared to the control plots, the Jungmeorijae restoration site was analyzed to have height growth of about 73.5%, the average species diversity index of about 75.2%. and the average similarity index was recovered to 7.75%. In the case of the restoration site in Jeungsimsa district, the height growth compared to the control plots was about 69.2%, the average species diversity index was about 55.0%. and the average similarity index was recovered to 25.65%. In the case of the Shimwon Valley ecological landscape restoration area, the height growth compared to the control plots was about 32.6%, the average species diversity index about 176.7%. and the average similarity index was recovered to 0.85%. The restoration site of the Jeungsimsa district was planted with relatively large trees during restoration work, and it took a relatively long time(20 years). Also, the site had less limiting factors due to the low elevation, allowing the degree of vegetation recovery to be higher than that of other sites.

• 한국산림과학회지
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• 제103권3호
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• pp.510-518
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• 2014

멧돼지는 농작물 피해, 도심 출몰 등으로 인해 인간과 갈등이 증가하고 있다. 유해야생동물에 대하여 주로 개체수 감소에 대한 노력이 진행되며, 생태적 가치에 대한 연구는 미미하다. 따라서 본 연구는 멧돼지의 비빔목 선택 및 생태학적 역할을 알아보기 위해 2013년 7월부터 10월까지 경남 거창군내 혼효림에서 실시되었다. 멧돼지 비빔목은 리기다소나무(Pinus rigida)가 가장 많이 발견되었으며, 침엽수와 활엽수 모두 해발 200~600 m에서 가장 많이 발견되었다. 흉고직경(DBH)에 있어 비빔목과 대조구 사이 유의한 차이가 나타나지 않았으며, 활엽수보다는 침엽수가 이용강도가 높은 것으로 나타났다. 비빔목 생태학적 특성을 파악하기 위해 반경 2.5 m 이내의 목본식물과 피도량을 대조구와 비교하였다. 총 25과 1,332종의 목본식물이 발견되었다. 각 층별 피도량에서 하층피도량과 중층피도량에서 나무 그룹별 통계적 유의성이 나타났고, 중상층과 상층에서는 나타나지 않았다. 또한 관목과 치수의 수에서 그룹별 유의한 차이가 나타났으며, 교목의 수에서는 나타나지 않았다. 총 개체수, 종수, 종다양도지수(H')에서는 모두 통계적 유의성이 나타났으며, 이용강도가 높은 비빔목에서 가장 높게 나타났다. 따라서 멧돼지는 주로 침엽수를 비빔목으로 선택하며, 이용강도가 높은 비빔목에서는 피도량 등 물리적 환경을 변화시켜 종다양도를 증가시키는 등 여러 가지 긍정적 영향을 끼치는 것으로 나타났다. 멧돼지의 산림생태학적 관리를 위해서는 매년 지속적인 포획보다는 3-4년을 주기로 강도 높은 개체수 감소 후 보전 정책이 필요할 것으로 판단된다.

• Animal Bioscience
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• 제37권8호
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• pp.1474-1482
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• 2024

Objective: The experiment aimed to determine the standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in 10 brown rice samples fed to pigs, and to construct predictive models for SID of CP and AA based on the physical characteristics and chemical composition of brown rice. Methods: Twenty-two cannulated pigs (initial body weight: 42.0±1.2 kg) were assigned to a replicated 11×3 incomplete Latin square design, including an N-free diet and 10 brown rice diets. Each period included 5 d adaptation and 2 d ileal digesta collection. Chromic oxide was added at 0.3% to all the diets as an indigestible marker for calculating the ileal CP and AA digestibility. Results: The coefficients of variation of all detected indices for physical characteristics and chemical composition, except for bulk weight, dry matter (DM) and gross energy, in 10 brown rice samples were greater than 10%. The SID of CP, lysine (Lys), methionine, threonine (Thr), and tryptophan (Trp) in brown rice was 77.2% (62.6% to 85.5%), 87.5% (80.3% to 94.3%), 89.2% (78.9% to 98.9%), 55.4% (46.1% to 67.6%) and 92.5% (86.3% to 96.3%), respectively. The best prediction equations for the SID of CP, Lys, Thr, and Trp were as following, SID_CP = -664.181+8.484×DM (R² = 0.40), SID_Lys = 53.126+6.031×ether extract (EE)+0.893×thousand-kernel volume (R² = 0.66), SID_Thr = 39.916+7.843×EE (R² = 0.41), and SID_Trp = -361.588+4.891×DM+0.387×total starch (R² = 0.85). Conclusion: Overall, a great variation exists among 10 sources of brown rice, and the thousand-grain volume, DM, EE, and total starch can be used as the key predictors for SID of CP and AA.

• 보존과학연구
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• 통권19호
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• pp.133-158
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• 1998

There are about 2,000 species of termite in the World, but one species of termite inhabits in the southern part of Korean peninsula. Termites are social insects that live in colonies. The colonies are composed of king, queen, soldiers and workers. Termite food consists of cellulose obtained from wood. Protozoa in their digestive tracts convert the cellulose into usable food. Korean termite is a subspecies of Reticulitermes speratus Kolbe, Rhinotermitidae. It's subterranean termite and the scientic name is R. speratus kyushensis Morimoto. The subterranean termite must nest in the soil in order to survival, and infest soft-wood which contact with the soil near the nest. There are several ways which subterranean termite infestations can be noticed. Atcertain times of the year during daylight hours, king and queen termites emerge from the colonies. The propose of these flight is to establish new colonies. The termite is a decomposer of biological ecosystem, but an invader in the preservation of cultural properties as like wood buildings. There are serveral control methods for the prevention of wood building from termite's damages. Those are biological control, ecological control, physical control and chemical control. Ecological and Physical control are the best methods in the new constructing wood-building. Fumigation which is a method of chemical control, is the best method for the building damaged by the termite. After the fumigation, we have totake wood & soil treatments for the building and nearby in order not to be reinvaded by the termites.

• Journal of Applied Biological Chemistry
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• 제55권1호
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• pp.27-34
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• 2012

Soy protein isolate (SPI)-based edible coating, with and without carboxymethyl cellulose (CMC), were used to reduce oxidative degradation of cut pork stored at $4^{\circ}C$ for 5 days. The SPI coating reduced (p<0.05) thiobarbituric acid-reactive substances (TBARS) and peroxide value (PV), compared with controls. The inhibition of TBARS and PV for SPI-coated porks with and without CMC, compared with the control was 19.1 and 23.9, and 25.7 and 37.7%, respectively. The SPI coating prevented loss of $L^*$ and $a^*$ values of porks compared to the control. The ability of the SPI coating to provide a moisture barrier for the porks was reduced (p<0.05). The SPI-coated porks with and without CMC reduced moisture loss by 37.3 and 44.6%, respectively, over the control. However, SPI coating of porks did not inhibit the growth of either total plate counts or L. monocytogenes. The result revealed that SPI can effectively be used as a natural antioxidative coating to extend quality and shelf life of pork.

• 지역사회간호학회지
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• 제23권3호
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• pp.327-337
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• 2012

Purpose: This study examined the effectiveness of an adolescent school -based smoking prevention program. Methods: The study was done with a nonequivalent control group pretest-posttest design. The subjects of the present study were 399 non-smoking high school students in Kyongbuk province. The subjects were divided into 184 experimental group members in G city who participated in a school-based smoking prevention program and 216 control group members in K city who did not participate. Data were collected from June to November 2011. collected data were analyzed using SPSS/WIN 18.0 through $x^2$-test, independent t-test and ANCOVA. Results: The experimental group had significantly increased knowledge of smoking, knowledge of smoking prevention program, attitude of smoking, skill of smoking prevention in comparison to the control group. Conclusion: The school-based smoking prevention program delivered to non-smoking adolescents is an effective method of encouraging smoking prevention and can be utilized as an effective nursing intervention for non-smoking adolescents.

• Journal of Ecology and Environment
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• 제40권1호
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• pp.5-11
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• 2016

Background: As the decomposition of lignocellulosic compounds is a rate-limiting stage in the nutrient mineralization from organic matters, elucidation of the changes in soil enzyme activity can provide insight into the nutrient dynamics and ecosystem functioning. The current study aimed to assess the effect of thinning intensities on soil conditions. Un-thinned control, 20 % thinning, and 30 % thinning treatments were applied to a Larix kaempferi forest, and total carbon and nitrogen, total carbon to total nitrogen ratio, extractable nutrients (inorganic nitrogen, phosphorus, calcium, magnesium, potassium), and enzyme activities (acid phosphatase, ${\beta}$-glucosidase, ${\beta}$-xylosidase, ${\beta}$-glucosaminidase) were investigated. Results: Total carbon and nitrogen concentrations were significantly increased in the 30 % thinning treatment, whereas both the 20 and 30 % thinning treatments did not change total carbon to total nitrogen ratio. Inorganic nitrogen and extractable calcium and magnesium concentrations were significantly increased in the 20 % thinning treatment; however, no significant changes were found for extractable phosphorus and potassium concentrations either in the 20 or the 30 % thinning treatment. However, the applied thinning intensities had no significant influences on acid phosphatase, ${\beta}$-glucosidase, ${\beta}$-xylosidase, and ${\beta}$-glucosaminidase activities. Conclusions: These results indicated that thinning can elevate soil organic matter quantity and nutrient availability, and different thinning intensities may affect extractable soil nutrients inconsistently. The results also demonstrated that such inconsistent patterns in extractable nutrient concentrations after thinning might not be fully explained by the shifts in the enzyme-mediated nutrient mineralization.

• 한국환경복원기술학회지
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• 제16권4호
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• pp.99-114
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• 2013

In this study, constructability of ecologocal stream restoration process was evaluated and improved approaches for habitat were suggested. The study site is Gucheon River in Geoje city where conducted ecologocal restoration work within maintaining its flood control function. Application of ecological design factors and constructability in the process from planning to construction was analyzed and its results are as follows: In the process of planning and designing, it was focused more on the naturality of Gucheon River rather than the human convinience and ecological design factors were applied within the ecological capacity of the site. First, the indexes for constructability evaluation is selected. It was classified into three major categories as construction quality, design quality and construction administration system. Each index has details, so there are twenty sub indexes for contractibility evaluation. Second, the evaluation results shows that the index most in need of improvement was plumbing construction, followed by Stone construction and pavement construction. design concept was evaluated as appropriate in entire categories. Finally it is suggested that it can be improved in both process of design to enhance the technology and process of construction to enhance the quality management. The constructability of ecological stream needs adaptive management and it must be discussed with its designing which is at the stage before construction. Also it needs discussion with its designer constantly and feed-back process.

• Animal cells and systems
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• 제16권4호
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• pp.329-336
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• 2012

This study evaluated ecological health, using various biomarkers and bioindicators, of pale chub (Zacco platypus) as a sentinel species, in Daejeon Stream, South Korea, during AprilMay 2011. The biomarkers and bioindicators were compared among three sites of control: Reference ($C_z$), transition ($T_z$), and the urban zones ($U_z$); and the 7-Ethoxyresorufin-O-deethylase (EROD) activity, DNA damage, acetylcholinesterase (AChE) activity, and vitellogenin (VTG) concentrations were more significantly increased in the $U_z$ than in the $C_z$. Also, physiological markers such as condition factor, liver somatic index, visceral somatic index, and gonad somatic index were significantly increased in the $U_z$ than in the $C_z$. For the health assessments, three categorized parameters of blood chemistry, molecular biomarkers, and physiological bioindicators were standardized and calculated as a star-plot, representing values of Integrated Health Response (IHR). Values of IHR had more significant (P<0.05) increases in the $U_z$ than any other zones, indicating an impairment of ecological health by organic matter, nutrients (N, P), and toxic chemicals. This study is based on low levels of biological organization approach of molecular and physiological biomarkers and bioindicators, so further study of high-levels of biological organization approach such as community and population is required for overall range of health assessments. The approach of IHR values, however, may be useful in providing early warning of future impacts on ecological health.