• 환경정책연구
• /
• 제13권3호
• /
• pp.3-19
• /
• 2014

급속한 경제성장으로 인한 대규모의 개발들은 산림, 농지 등 넓은 면적의 야생 동식물의 서식지의 훼손 및 단절을 유발하였다. 이와 같은 문제를 해결하기 위해 국토의 자연생태계를 하나의 유기체로서 인식하고, 생태적으로 우수한 서식지의 지속적인 관리를 통하여 자연생태계를 보전해야 한다. 특히 지자체에서는 생태적 연결성뿐만 아니라 환경생태적 우수성까지 고려한 광역생태네트워크를 구축하여 지자체 개발관련 계획에 적극 반영해야 한다. 이를 위해 국토의 생태적 연결성을 평가한 광역생태축과 우리나라의 환경생태적 가치를 정량적으로 분석하고 평가하기 위한 평가지도인 국토환경성평가지도의 환경생태적 평가 결과를 활용하여 지자체 광역생태네트워크 구축 방안을 제시하였다. 그리고 경상남도 사례로 지자체 광역생태네트워크를 구축하였으며, 구축 결과 기존에 구축되었던 광역생태축의 핵심지역 $2,986km^2$과 완충지역 $2,940km^2$가 확장되어 핵심지역은 $4,049km^2$, 완충지역은 $3,006km^2$으로 결과가 도출되었다. 개발계획 시 이러한 광역생태네워크의 참조는 지자체 생물다양성 증진과 생태계 보호를 포함한 지자체 통합적 환경관리에 크게 기여할 수 있을 것으로 판단된다.

• Entomological Research
• /
• 제48권6호
• /
• pp.533-539
• /
• 2018

The RNA interference (RNAi) has been considered as an important genetic tool and applied to develop a new living modified (LM) crop trait which is an improvement of nutrient quality or pest management. The RNAi of DvSnf7 has been used for resistance to LM maize and the Western Corn Rootworm which is a major agricultural pest for the US Corn Belt. Most of the environmental risk assessments (ERA) of double strand RNA (dsRNA) have been performed using in vitro transcript products, and not in vivo expressed product. A large amount of dsRNA was required for the acute toxicity assay of water fleas. Therefore development of massive dsRNA purification techniques is critical. Daphnia, a freshwater microcrustacean, is a model organism for studying cellular and molecular mechanism involved in life history traits and ecotoxicology. In this study, we established the massive dsRNA purification method using Escherichia coli and implemented acute toxicity assays to Daphnia magna. As a result, the present RNase A and DNase I, dsRNA was efficiently purified without any special techniques or equipment. Even though purified dsRNA existed during the acute toxicity test, lethality or abnormal behavior were not observed in D. magna. These results indicated that GFP and DvSnf7 dsRNA were not significantly affected to D. magna due to their lack of sequence matching in its genome. The purification method of dsRNA and the acute toxicity assay of water fleas using purified dsRNA would be suitable for the toxicological studies of LMOs to aquatic non-target organisms.

• Journal of Ginseng Research
• /
• 제43권4호
• /
• pp.600-605
• /
• 2019

Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositions of the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years, the aerial parts of members of the Panax genus have received great attention from natural product chemists as producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation of novel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro. Methods: Various chromatographic techniques were applied to obtain pure individual compounds, and their structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry, as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated on lipopolysaccharide-stimulated RAW 264.7 cells. Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside $LS_1$ (1) and 5,6-didehydroginsenoside $Rg_3$ (2), were isolated from the leaves of P. ginseng. The isolated compounds 1 and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 cells, and Compound 2 showed a significant inhibitory effect with $IC_{50}$ of $37.38{\mu}M$ compared with that of NG-monomethyl-L-arginine ($IC_{50}=90.76{\mu}M$). Moreover, Compound 2 significantly decreased secretion of cytokines such as prostaglandin $E_2$ and tumor necrosis factor-${\alpha}$. In addition, Compound 2 significantly suppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food, and the mechanism is warranted for further exploration.

• 한국조경학회지
• /
• 제26권1호
• /
• pp.96-112
• /
• 1998

This study aims to evaluate the potential of pro-environmental application(PEP) in streams conceptualized ecological conservation and recreational use to be in harmony with. The main content of research are to clarify the PEP, to establish the evaluation model, and to evaluate 3 streams(Dongchon, Seokhyunchon, Okchon) in Sunchon city. Researchers introduced 12 evaluation items(water quality, water quantity, vegetation-water area, vegetation conservation, streamscape, neighborhood landscape, stream width, optimum area, nearby landuse, facility in stream, distance from user, obstacle to acces) by 5 scales to evaluate the characteristics of natural and artificial factors in stream area and nearby area. Also to decide the weight of items, researchers surveyed the opinion of 22 landscape architects experienced stream-plan through delphi method. Lastly the pro-environmental potential on streams were calculated by the ecological potential and recreational potential indices to be standardized and indicidual sections in streams were divided 5 grades on the basis of PEP. The result of this study are as follows; 1) The evaluation model of PEP was constructed by 4 steps, such as the decision of weight, the measurement of scale, the calculation of potential indices, the gradation of individual sections in streams. 2) The ecological potential were highly influenced by natural factor such as water quality, vegetation conservation, vegetation-water area, but on the other hand the recreational potential were influenced by optimum area, distance from user, water quantity, obstacle to access. 3) The factors such as vegetation conservation, optimum area, nearby landuse, distance from user were function as discernment factors to evaluate relatively ecological and recreational potential. and water quality, water quantity, vegetation -water area, neighborhood landscape were acted as important items to decide PEP.

• 한국환경복원기술학회지
• /
• 제22권6호
• /
• pp.97-114
• /
• 2019

This study aims to analyze the effect of urban ecosystem restoration projects by evaluating the short-term restoration performance of the project sites, from both qualitative and quantitative evaluations. In this study, for the qualitative evaluation, we derived the evaluation frame from previous studies and literature. For the quantitative evaluation, the changes in ecological connectivity after the restoration project were described using landscape permeability and network analysis. In addition, changes in habitat quality after the restoration project were evaluated by using InVEST Habitat Quality Model. These evaluations were applied to the three natural madang (ecological restoration) projects and two ecosystem conservation cooperation projects. As a result, three categories, 10 indicators, and 13 sub-indicators were derived from literature as the evaluation frame for this study. In the case of quantitative evaluation of restoration performance, habitat quality increased by 45% and ecological connectivity by 37% in natural-madang, and habitat quality by about 12% and ecological connectivity by about 19% in ecosystem conservation cooperation projects. This implies that the ecological restoration project can increase the ecological connectivity and the habitat quality of degraded sites even in a short period of time by improving the land-cover and land use. The results by applying the evaluation frame indicated that ecological and environmental factors and the ecological functions were improved by the restoration works, even though the magnitude of performances were diverse depending on the specific evaluation items, project type, and site characteristics. This study clarified that the success of ecological restoration project should be assessed by both of the short-term and long-term goals, which can be achieved by the maintenance and sustainable management, respectively.

• Journal of Plant Biotechnology
• /
• 제43권1호
• /
• pp.91-98
• /
• 2016

면화(cotton)는 섬유를 수확하고, 면실유는 식용으로 가공 후 남은 것은 다시 사료로 이용되어 다방면으로 다양하게 활용되는 작물이다. LM 면화는 옥수수, 대두, 캐놀라와 달리 아시아권(중국, 인도 등)에서 가장 많이 재배되고 있으며, 우리나라는 2013년까지 세계 1위의 LM 면실(사료용) 수입국이며, 세계 4위의 면실박 수입국으로 해마다 증가하는 LM 면화의 수입량과 맞물려 유통 및 소비과정에서의 비의도적으로 유출 가능성이 증가함에 따라 LM 면화의 자연생태계 위해성 평가 및 안전관리가 요구된다. 본 연구에서는 국내 수입 승인 LM 면화 6개 이벤트(MON15985, MON531, GHB614, LLCOTTON25, MON88913, MON1445)의 동시증폭 검출법(multiplex PCR)을 개발하여 보다 신속하고 명확한 검출기법을 확립하고자 하였다. 최적 multiplex PCR 반응 조건은 2개 LM 면화 이벤트 MON15985 (214 bp), MON531 (270 bp)와 4개 LM 면화 이벤트 GHB614 (119 bp), LLCOTTON25 (164 bp), MON88913 (276 bp), MON1445 (389 bp)가 한번의 반응에 명확하게 검출되는 최적 반응 조건 및 primer 반응 농도의 조절을 통해 서로 다른 생성물 크기로 명확히 구분되도록 하였고, 최적 primer 농도는 반응액 최종농도 0.2~0.66 pmol로 primer 쌍 마다 각각 다른 최적 농도 조합의 cocktail을 만들어 활용하였다. Duplex PCR 반응 조건은 초기 $95^{\circ}C$ 5분 반응 후, $95^{\circ}C$ 15초, $55^{\circ}C$ 20초 15회 반응하고, 다시 $95^{\circ}C$ 15초, $60^{\circ}C$ 20초 25회 반응하였을 때 최적 검출이 이루어졌고, tetraplex PCR 반응 조건은 $95^{\circ}C$ 5분 반응 후, $95^{\circ}C$ 15초, $60^{\circ}C$ 20초 50회 반응하였을 때 최적 검출이 이루어졌다. 본 연구에서 개발된 multiplex PCR 검출법은 국내 수입 유통 LM 면화의 자연환경 모니터링에 활용함에 있어 요구되는 연구인력, 시간 및 비용을 보다 효율적으로 개선하는데 적용될 수 있을 것으로 사료된다.

• 환경영향평가
• /
• 제18권1호
• /
• pp.51-57
• /
• 2009

Corylopsis coreana Uyeki is endemic species in the Korean peninsula and is designated a Category Endangered Plant Species by the Wildlife Protection Act of South Korea. We developed the plan and cooperative model within the local community for the species conservation. In order to carry out this plan we first investigated the ecological characteristics of the species. The species shows patterns of discontinuous distribution and is coupled with the unusual feature of only growing on northern exposed slopes. Although Corylopsis coreana is cut the stem every year, many new sprouts are still grown from the root. Natural germination of the seed occurs only on north-facing slopes, but not on south-facing slopes at spring. That is, the species is highly influenced by soil moisture until the seedling stage has been reached. This factor limits the distribution of the species. When saplings are planted on south-facing slopes, they grow well. The information we gathered greatly helped with efforts to draw up conservation plans. In addition, when the information was shared with the local community, builders and residents showed great interest and displayed a will to help with conservation efforts. Therefore, a cooperative model within the local community was drawn up for the conservation of the species. Accordingly this model could be applied at mitigation measure at environment impact assessment.

• 한국환경복원기술학회지
• /
• 제9권5호
• /
• pp.41-49
• /
• 2006

Private land within national parks has raised acute questions for impact assessment practitioners as to whether it is ecologically resourceful enough to be conserved or whether it should be removed from national parks and compensated accordingly. The purpose of this study is to provide a framework for spatial decision support through assessing ecological resource of private land within national parks. In order to assess the ecological resource, private land within national parks was analyzed and quantified based upon topography, flora and fauna. National parks, according to the ecological resource assessment, have been divided into three groups : the parks in need of careful attention for conservation; the parks needed to be retained as buffers, and; the parks that can be released to private land. According to the analysis, part of Mountain Joowang is the third-tier land that does not require conservation efforts. This case study intends to help policy-makers decide whether some private land within national parks can be released and, after the release, what should be done to prevent reckless development of the released land.

• 환경영향평가
• /
• 제17권2호
• /
• pp.133-141
• /
• 2008

The loss, alteration, and fragmentation of habitat have led to a reduction of biodiversity. The growing awareness of the negative effects of habitat fragmentation on natural systems has resulted in conservation strategy that is concerned with not only population and habitat level but also ecosystem and landscape level. Especially, ecological network to link core areas or major habitat patches is one of the most important issues. Recently, landscape connectivity is increasingly used in decision making for fragmented landscape management in order to conserve the biodiversity in the regional scale. The objective of this study was to find potential forest as a ecological corridor in Go-yang city, Gyung-gi province using cost-distance modelling method that can measure connectivity based on animal movement. 'Least cost-distance' modelling based on functional connectivity can be useful to establish ecological network and biodiversity conservation plan. This method calculates the distance modified with the cost to move between habitat patches based on detailed geographical information on the landscape as well as behavioural aspects of the animal movement. The least cost-distance models are based on two biologically assumptions: (1) dispersers have complete knowledge of their surroundings, and (2) they do select the least cost route from this information. As a result of this study, we can find wildlife moving route for biodiversity conservation. The result is very useful for long-term aspect of biodiversity conservation plan in regional scale, because this is reflection of geographical information and behavioural aspects of the animal movement.

• 한국환경복원기술학회지
• /
• 제22권2호
• /
• pp.73-92
• /
• 2019

The purpose of this study is to establish the conservation and restoration areas for sustainable stream management by reflecting the ecological health, cultural characteristics, and the citizens' needs for stream uses. Therefore, we extended spatial ranges of stream evaluation to riparian areas in addition to stream sections. The evaluation indexes are stream naturalness, availability, biota assessment, and riparian characteristics such as land uses and legally protected areas. The grading system was unified with five classes. The spatial evaluation units for stream section are classified as right and left for laterally and 500m for longitudinally. For riparian areas, 30m and 100m of distance from the edge of stream are applied as spatial evaluation units. The six types of stream sections for management are conservation sections(priority/general) and restoration sections(priority/general) for ecological landscape and core and general sections for cultural landscape. The established system for evaluation and designating areas for stream management was applied to Jeonjucheon and Samcheon in Jeonju-si, and the conservation and restoration areas were designated reflecting the characteristics of urban and non-urban areas and left and right of stream sections. The results of this study will provide detailed guidelines for designating stream sections and practical management strategies for sustainable urban stream management.